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The map label for this gene is oprM [H]

Identifier: 91784639

GI number: 91784639

Start: 3629619

End: 3631154

Strand: Reverse

Name: oprM [H]

Synonym: Bxe_A1155

Alternate gene names: 91784639

Gene position: 3631154-3629619 (Counterclockwise)

Preceding gene: 91784640

Following gene: 91784638

Centisome position: 74.17

GC content: 65.17

Gene sequence:

>1536_bases
ATGACGTCACAACCGCTGCTCTCACAACTGCTGCGACAGGCAGGCACGGCACTCTGCACGATCTCCCTGGTCGGTTGCGC
GGTTGGTCCTGACTTCGTGAAACCCGACGACAGGCTCGCAGCCGTTCAACTGTCGCCCAGGGCGGACTACGCGCAGGCTC
AGGAGACATCCGCCGCGAACTTCCCGTCTTCATGGTGGACGTTGTTCAACGATGACGTACTGGCGGATCTGCAAGCCCGT
GCGCAGGCGGGCAACCTGAACCTGCAGATGGCTTCCGAGCGCATCGAGCAAAGTCGGGCGCAGTTGGGGATTGCCTCCTC
GCTGCTGCTGCCCAGTGTGGGTGCTTCGGCGGGCTATTCCCGCGAGGCCATCAGCGAGCACGGCAAGTTTGCCGCGTTGG
GTGCTCCGACGAGCGCGAGCGACTTCTGGCAGTTGGGATTCGACGCCAGTTGGGAACTCGACCTATGGGGCCGCGCGCGT
CGATCGCGCGAAAGCGCCGCGGCCGCCTTCGAGGCCACGGTGTATGAGCGCGAGGCGGCACGCGTGGCCCTGTCCGCCGA
GGTGGCCCGTACCTATCTCCAATTGCGCGGCACGCAAGCGCAACTGGACATCACCCGGCAGAACCTGGAGGTTGCCGATC
GCACGCTCCGCCTTGCCGAAAGCCGTGAGCGCAATGGCGTCGCCACACGTTTTGAAACCGCTTCGGCGCGCGCGCAACTG
GCAACGGTCGAGGCGCTGGTACCCGAATTGGTCCAGCGCCGCAACACGCTGATGAACGTCCTGGCATTGCTGCTGGGAGA
GCAGCCGCGCGCCCTCGATGCGCAGTTGCTTGAAGCGATGCCTCTGCCGTCGCTTCCTTCCAATGTGCCGGTGGGCCTGC
CCTCCGAATTGGCGCACAGGCGGCCGGACATACAGCGCGCCGAAGCCCAGCTCCATGCTGCGACGGCAGCCATCGGCGTC
GCCAAAGCCGATTTCTACCCGCGAATCGGCCTCAAAGGCAGGGTCGGCGTCGAAGCCTTCGAGTTCAACGACCTCGACAG
TTGGGATTCCCGGTTCTTCTCCGTCGGCCCCACGGTCTATCTTCCCATCTTCCAGGGCGGGCGTCTGAAGCAGCGCCTGG
CGCTGAACGAGGCACGGCAAAGAACTGCCGCCATCGGCTACCGGCAGACGGTGCTGCAAGCCTGGCATGAAGTGGACAAC
GCCCTGGACGCCTGGGCAGCCCAGCAGAACCGCCATGCCGAGTTGGTGGTTTCGTACGAGCAGAACCAGCAGGCGCTGCA
TGCTGCCGAGCGCGGCTATCAGGAAGGCGCTGCCGACTACCTGAACGTCCTGGCCGCACAGCGCGGTGTCCTCGCAAGCC
AGACCAGCCTCAATGCCAGCGCGACCAACGCCGCGCTCACCCTGGTCAATCTCTACAAATCCCTGGGCGGTGGCTGGGAC
CCCGGCGCATTCTCTTCCGAGCAGCAGACCGGCGGCGACGCGTCGCTGACGACCGCTGTGTCGTCGGCCACCTCGCAATC
GAAGGTTGACCAATGA

Upstream 100 bases:

>100_bases
AAGTTATGAGGATGCTCGGAATTTATTTGAACTGCTGGTTAACTTTTCTTTTATGTCAAACCGAGCGGTTCGGTACTATT
CGTATATTGGGTGAACTTTC

Downstream 100 bases:

>100_bases
GCGCCGCAGCGTCGCAGTCGTCGACGGCGCCGCCGCCCGCGGCAACCGCTGCGGCAGCCCCTCCGAAGCGGGCGATTGCG
GGACTGGTCGGCATCCTCAT

Product: MFS efflux system, outer membrane porin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MTSQPLLSQLLRQAGTALCTISLVGCAVGPDFVKPDDRLAAVQLSPRADYAQAQETSAANFPSSWWTLFNDDVLADLQAR
AQAGNLNLQMASERIEQSRAQLGIASSLLLPSVGASAGYSREAISEHGKFAALGAPTSASDFWQLGFDASWELDLWGRAR
RSRESAAAAFEATVYEREAARVALSAEVARTYLQLRGTQAQLDITRQNLEVADRTLRLAESRERNGVATRFETASARAQL
ATVEALVPELVQRRNTLMNVLALLLGEQPRALDAQLLEAMPLPSLPSNVPVGLPSELAHRRPDIQRAEAQLHAATAAIGV
AKADFYPRIGLKGRVGVEAFEFNDLDSWDSRFFSVGPTVYLPIFQGGRLKQRLALNEARQRTAAIGYRQTVLQAWHEVDN
ALDAWAAQQNRHAELVVSYEQNQQALHAAERGYQEGAADYLNVLAAQRGVLASQTSLNASATNAALTLVNLYKSLGGGWD
PGAFSSEQQTGGDASLTTAVSSATSQSKVDQ

Sequences:

>Translated_511_residues
MTSQPLLSQLLRQAGTALCTISLVGCAVGPDFVKPDDRLAAVQLSPRADYAQAQETSAANFPSSWWTLFNDDVLADLQAR
AQAGNLNLQMASERIEQSRAQLGIASSLLLPSVGASAGYSREAISEHGKFAALGAPTSASDFWQLGFDASWELDLWGRAR
RSRESAAAAFEATVYEREAARVALSAEVARTYLQLRGTQAQLDITRQNLEVADRTLRLAESRERNGVATRFETASARAQL
ATVEALVPELVQRRNTLMNVLALLLGEQPRALDAQLLEAMPLPSLPSNVPVGLPSELAHRRPDIQRAEAQLHAATAAIGV
AKADFYPRIGLKGRVGVEAFEFNDLDSWDSRFFSVGPTVYLPIFQGGRLKQRLALNEARQRTAAIGYRQTVLQAWHEVDN
ALDAWAAQQNRHAELVVSYEQNQQALHAAERGYQEGAADYLNVLAAQRGVLASQTSLNASATNAALTLVNLYKSLGGGWD
PGAFSSEQQTGGDASLTTAVSSATSQSKVDQ
>Mature_510_residues
TSQPLLSQLLRQAGTALCTISLVGCAVGPDFVKPDDRLAAVQLSPRADYAQAQETSAANFPSSWWTLFNDDVLADLQARA
QAGNLNLQMASERIEQSRAQLGIASSLLLPSVGASAGYSREAISEHGKFAALGAPTSASDFWQLGFDASWELDLWGRARR
SRESAAAAFEATVYEREAARVALSAEVARTYLQLRGTQAQLDITRQNLEVADRTLRLAESRERNGVATRFETASARAQLA
TVEALVPELVQRRNTLMNVLALLLGEQPRALDAQLLEAMPLPSLPSNVPVGLPSELAHRRPDIQRAEAQLHAATAAIGVA
KADFYPRIGLKGRVGVEAFEFNDLDSWDSRFFSVGPTVYLPIFQGGRLKQRLALNEARQRTAAIGYRQTVLQAWHEVDNA
LDAWAAQQNRHAELVVSYEQNQQALHAAERGYQEGAADYLNVLAAQRGVLASQTSLNASATNAALTLVNLYKSLGGGWDP
GAFSSEQQTGGDASLTTAVSSATSQSKVDQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=475, Percent_Identity=28.6315789473684, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI2367348, Length=487, Percent_Identity=28.1314168377823, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55171; Mature: 55040

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQPLLSQLLRQAGTALCTISLVGCAVGPDFVKPDDRLAAVQLSPRADYAQAQETSAAN
CCCCHHHHHHHHHHCCCEEHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCC
FPSSWWTLFNDDVLADLQARAQAGNLNLQMASERIEQSRAQLGIASSLLLPSVGASAGYS
CCCHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
REAISEHGKFAALGAPTSASDFWQLGFDASWELDLWGRARRSRESAAAAFEATVYEREAA
HHHHHHCCCEEEECCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
RVALSAEVARTYLQLRGTQAQLDITRQNLEVADRTLRLAESRERNGVATRFETASARAQL
HHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
ATVEALVPELVQRRNTLMNVLALLLGEQPRALDAQLLEAMPLPSLPSNVPVGLPSELAHR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHC
RPDIQRAEAQLHAATAAIGVAKADFYPRIGLKGRVGVEAFEFNDLDSWDSRFFSVGPTVY
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHEECCCEEE
LPIFQGGRLKQRLALNEARQRTAAIGYRQTVLQAWHEVDNALDAWAAQQNRHAELVVSYE
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
QNQQALHAAERGYQEGAADYLNVLAAQRGVLASQTSLNASATNAALTLVNLYKSLGGGWD
CCHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCC
PGAFSSEQQTGGDASLTTAVSSATSQSKVDQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSQPLLSQLLRQAGTALCTISLVGCAVGPDFVKPDDRLAAVQLSPRADYAQAQETSAAN
CCCHHHHHHHHHHCCCEEHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCC
FPSSWWTLFNDDVLADLQARAQAGNLNLQMASERIEQSRAQLGIASSLLLPSVGASAGYS
CCCHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
REAISEHGKFAALGAPTSASDFWQLGFDASWELDLWGRARRSRESAAAAFEATVYEREAA
HHHHHHCCCEEEECCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
RVALSAEVARTYLQLRGTQAQLDITRQNLEVADRTLRLAESRERNGVATRFETASARAQL
HHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
ATVEALVPELVQRRNTLMNVLALLLGEQPRALDAQLLEAMPLPSLPSNVPVGLPSELAHR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHC
RPDIQRAEAQLHAATAAIGVAKADFYPRIGLKGRVGVEAFEFNDLDSWDSRFFSVGPTVY
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHEECCCEEE
LPIFQGGRLKQRLALNEARQRTAAIGYRQTVLQAWHEVDNALDAWAAQQNRHAELVVSYE
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
QNQQALHAAERGYQEGAADYLNVLAAQRGVLASQTSLNASATNAALTLVNLYKSLGGGWD
CCHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCC
PGAFSSEQQTGGDASLTTAVSSATSQSKVDQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]