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The map label for this gene is emrA [H]

Identifier: 91784637

GI number: 91784637

Start: 3627006

End: 3628040

Strand: Reverse

Name: emrA [H]

Synonym: Bxe_A1157

Alternate gene names: 91784637

Gene position: 3628040-3627006 (Counterclockwise)

Preceding gene: 91784638

Following gene: 91784636

Centisome position: 74.1

GC content: 62.9

Gene sequence:

>1035_bases
ATGGCCATGCCGAAAAAAGCCAAGATCACCAGTGCCGTGCTGCTCCTCGCAGTGGCAGTGGGCTGTGTCATTTATCTGAA
TCGCCCCGAATCCAGCGCATCGACCCAATCCACGGACGACGCCTATGTACAGGCCGATTTCACCCTCGTCGCCCCCCAGG
TGTCGGGAGTGATCGGCAAGGTGCTGGTCGAAGAAAACCAACCAGTGAAAGCCGGGGATCTGCTTGCGGCCATCGACGAT
CGAGACTTCATCGTTGCGGTGGACGCGGCAAAGGCACAAGTCGCCGCCGCGAAGGCCAGCGTCGCGGGGCTTGCCGCGCG
CGTGGCGCAGCAGGAGACCGCCATACGTCAGGCTCAGGCGACAGTCGCGGTGGACGACGCAGAACTCAAATTGGCAGAGG
CCAACCAAAGGCGTTATCGGAACCTGGCTTCAGACGGATCAGGTTCGATCCAAGCGCGCCAACAGGCCGAGGCGCAGTTG
GCCATTCGGCTGGCCAGCCGAGACAGGAACCGCGCGGGCCTGCAAGCGGCACGGCAACAGACGGACATCCTCAAAGCCGA
TCTTGAAAAAGCCAAGGCTGCACTTTCCCAAGCGCAGGCGGCGCAGGACGCGGCGGAGTTGAAGCTCTCCTATACCCAAA
TCACAGCGCCCATCAGCGGCCTCATCGGTCAGAAGTCAGTGCGCGTCGGCGCATTCGTGAATGCTGGCAAGCCGCTGGTG
GCGGTGATCCCGCTCGATGCGGTCTACATCACGGCCAACTTCCGCGAAACGCAATTGGCACGTGTGCAGCCAGGGCAGTC
GGTGGACATCAAGGTGGACGCCTTGCCGGGGGCCGTACTGAAGGGCACCGTGGAGAGCCTCGGACCGGCCAGTGGCGTCA
GCTACTCGGCCGTCGCGCCACACAACGCCACGGGCAACTTCACCAAGATCGTCCAGCGCCTACCCGTGCGCATTCGCATT
GACCCCGACCAGCCTGGTGCCGCGAATTTGCGCGTCGGTATGTCCGTGACGCCGAGGATCCACATCGGTGAATAG

Upstream 100 bases:

>100_bases
GTGATGGTCCCCTTCGTATTGCGACTGACCTACATTCCCGCTCCCATTTTGCAGGCCCCCGCTTCGCAATCCACATCGTC
GTCCCAAGGATAGTTCCGCC

Downstream 100 bases:

>100_bases
CGCCATTCGTCCAGCATGAGGAGCCATACAAATGAGGCAATACCGATATCGCGTAACTGTGGAGCAGTTGACTGATTCGC
ATGGCATCCCCAGCGATCGC

Product: major facilitator superfamily multidrug efflux pump membrane fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MAMPKKAKITSAVLLLAVAVGCVIYLNRPESSASTQSTDDAYVQADFTLVAPQVSGVIGKVLVEENQPVKAGDLLAAIDD
RDFIVAVDAAKAQVAAAKASVAGLAARVAQQETAIRQAQATVAVDDAELKLAEANQRRYRNLASDGSGSIQARQQAEAQL
AIRLASRDRNRAGLQAARQQTDILKADLEKAKAALSQAQAAQDAAELKLSYTQITAPISGLIGQKSVRVGAFVNAGKPLV
AVIPLDAVYITANFRETQLARVQPGQSVDIKVDALPGAVLKGTVESLGPASGVSYSAVAPHNATGNFTKIVQRLPVRIRI
DPDQPGAANLRVGMSVTPRIHIGE

Sequences:

>Translated_344_residues
MAMPKKAKITSAVLLLAVAVGCVIYLNRPESSASTQSTDDAYVQADFTLVAPQVSGVIGKVLVEENQPVKAGDLLAAIDD
RDFIVAVDAAKAQVAAAKASVAGLAARVAQQETAIRQAQATVAVDDAELKLAEANQRRYRNLASDGSGSIQARQQAEAQL
AIRLASRDRNRAGLQAARQQTDILKADLEKAKAALSQAQAAQDAAELKLSYTQITAPISGLIGQKSVRVGAFVNAGKPLV
AVIPLDAVYITANFRETQLARVQPGQSVDIKVDALPGAVLKGTVESLGPASGVSYSAVAPHNATGNFTKIVQRLPVRIRI
DPDQPGAANLRVGMSVTPRIHIGE
>Mature_343_residues
AMPKKAKITSAVLLLAVAVGCVIYLNRPESSASTQSTDDAYVQADFTLVAPQVSGVIGKVLVEENQPVKAGDLLAAIDDR
DFIVAVDAAKAQVAAAKASVAGLAARVAQQETAIRQAQATVAVDDAELKLAEANQRRYRNLASDGSGSIQARQQAEAQLA
IRLASRDRNRAGLQAARQQTDILKADLEKAKAALSQAQAAQDAAELKLSYTQITAPISGLIGQKSVRVGAFVNAGKPLVA
VIPLDAVYITANFRETQLARVQPGQSVDIKVDALPGAVLKGTVESLGPASGVSYSAVAPHNATGNFTKIVQRLPVRIRID
PDQPGAANLRVGMSVTPRIHIGE

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=306, Percent_Identity=32.0261437908497, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1789041, Length=333, Percent_Identity=31.5315315315315, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1790519, Length=307, Percent_Identity=30.9446254071661, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789637, Length=322, Percent_Identity=28.8819875776398, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081951, Length=339, Percent_Identity=27.1386430678466, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790024, Length=274, Percent_Identity=27.3722627737226, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790012, Length=249, Percent_Identity=29.3172690763052, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789900, Length=352, Percent_Identity=25.5681818181818, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 35978; Mature: 35847

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMPKKAKITSAVLLLAVAVGCVIYLNRPESSASTQSTDDAYVQADFTLVAPQVSGVIGK
CCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEEEEEECCHHHHHHHH
VLVEENQPVKAGDLLAAIDDRDFIVAVDAAKAQVAAAKASVAGLAARVAQQETAIRQAQA
EEECCCCCCCCCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
TVAVDDAELKLAEANQRRYRNLASDGSGSIQARQQAEAQLAIRLASRDRNRAGLQAARQQ
EEEECCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHH
TDILKADLEKAKAALSQAQAAQDAAELKLSYTQITAPISGLIGQKSVRVGAFVNAGKPLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHEECCCHHHHCCCCCEEEEEEEECCCCEE
AVIPLDAVYITANFRETQLARVQPGQSVDIKVDALPGAVLKGTVESLGPASGVSYSAVAP
EEEECCEEEEEECCCCCEEEECCCCCCEEEEEECCCCHHHHCCHHHCCCCCCCCEEEECC
HNATGNFTKIVQRLPVRIRIDPDQPGAANLRVGMSVTPRIHIGE
CCCCCCHHHHHHHCCEEEEECCCCCCCCCEEECCEECCEEEECC
>Mature Secondary Structure 
AMPKKAKITSAVLLLAVAVGCVIYLNRPESSASTQSTDDAYVQADFTLVAPQVSGVIGK
CCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEEEEEECCHHHHHHHH
VLVEENQPVKAGDLLAAIDDRDFIVAVDAAKAQVAAAKASVAGLAARVAQQETAIRQAQA
EEECCCCCCCCCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
TVAVDDAELKLAEANQRRYRNLASDGSGSIQARQQAEAQLAIRLASRDRNRAGLQAARQQ
EEEECCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHH
TDILKADLEKAKAALSQAQAAQDAAELKLSYTQITAPISGLIGQKSVRVGAFVNAGKPLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHEECCCHHHHCCCCCEEEEEEEECCCCEE
AVIPLDAVYITANFRETQLARVQPGQSVDIKVDALPGAVLKGTVESLGPASGVSYSAVAP
EEEECCEEEEEECCCCCEEEECCCCCCEEEEEECCCCHHHHCCHHHCCCCCCCCEEEECC
HNATGNFTKIVQRLPVRIRIDPDQPGAANLRVGMSVTPRIHIGE
CCCCCCHHHHHHHCCEEEEECCCCCCCCCEEECCEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]