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The map label for this gene is hyuA [H]

Identifier: 91784327

GI number: 91784327

Start: 3289468

End: 3291531

Strand: Reverse

Name: hyuA [H]

Synonym: Bxe_A1473

Alternate gene names: 91784327

Gene position: 3291531-3289468 (Counterclockwise)

Preceding gene: 91784329

Following gene: 91784326

Centisome position: 67.23

GC content: 65.55

Gene sequence:

>2064_bases
ATGCTTAAGATTGGCGTTGATATTGGCGGTACGTTCACCGATTTCGCGGCCTGGCGAGAAGGCGGCGAGGGGCGCGTGGA
AACGTTGAAAGTGCCGTCCACACCTCCGGATTTCGCCGAAGCCTTCAAGTCGGGCTGCAATGAAATACTGAAACGGATGG
AGCCGCACACGCACGAGGAAATCGTGGTCGTGCACGGCACGACAGTGGCCACCAACACCGTGATCGAGCGCAATGGTCCC
TCTATTGCACTGTTCACGACGGAGGGCTTTCGCGACATCCTCGAATTGCAGCGACTGCGTTTGCGCCGCGCGATCGATCT
GTTCGGCAACCGGGTGGACCCGCTGATTCCGCGCGAACTCGTGTTTGAAATTCCGGAGCGGAGCGGATCGGATGGACGTA
TGCGCACGCGGCTCGACCTGGACGCGGTCAAGCATGCGGCGCACGCAGCGAAAGAGGCGGGAGTGGAAGGCATCGCGATT
GCCTTCCTGCATAGCTTTCGCAATCCGTCGCATGAAATCGCGGCACGCAAGGCCGTGATCGAAGCGACGGGCTTGCGCAA
CGTGTCGATCTCCAGCGAGATCTGGCCAAAGATCGGCGAGTACGAACGGGCGATCGTCGCGGTGCTCAACACATACGTGA
AGCCGCGTATGAGCGCTTACATCGGCGAGATCGAACGTTGGCTGGCCGCGCGTCTGCCGAATGCGAAGCTCTTCATCATG
CAGTCGAACGGCGGCGCACTCTCTGCCGACGAGGCGCGCGAATTGCCCGTCCATACGCTGCTGTCGGGCCCGGCCTCCGG
CGTGACGGCGGCGCAAAGCCTTGGTGCCGCGCTGAACGAGCGCTGCATGTTGACGATGGACATGGGCGGTACGAGCACCG
ATCTCTCGCTGATTCAGGACAACGAGCCGTCGATCACCGGCGACGCCGAAGTAGGCGACTTCCCGCTGATGATGCCGGTC
ACCGGCATCGAAGCGATCGGCGCGGGCGGCGGCTCGGTCTGCTGGATCGACGGCGGAGTGCTGCGGGTCGGCCCGAAAAG
CGCGGGTGCGCGTCCGGGTCCGGCATGCTTCGGCCACGGCGGCACGCTGCCGACGGTGACCGACGCATACCTGCTGTGCG
GTTTCATCGATCCGCTGCATTTTCTCGGCGGACGCATGACGCTCGACGTCGACGCCGCACATGCCGCAATGCAGCCCGTG
GCCGACGCATTGAAGATGGACGTCGTGTCCGCGGCGCAGGCCTGCCTCACGGTGGCGACGTCGAACATGGTGGCGAGCGT
GCTGCCGTATCTGGCGCGCTATGGCCTCGATCCCGCCGACGTGACGCTGGTGGTCTACGGCGGCGCGGGCTCGCTGCACG
GCCCGCTGCTGGCGACGGAACTGGGCATCGGGCGCGTGCTGGTGCCGGGCATGCCTTCGGTGTTCTGCGCATATGGCGGC
CTGGTCGCCGGCCTCACGCACGACGCCGTGAAGAGCGTGCAGGGCGTGACAGTGGACGGCGCCCTGATCCAGCGCGAATT
CGCCGGCCTCGAACAGAGTGCGCGTGAATGGCTCGCGAGCCAGGACGTCGGCGTGAGTCTGCGCGAGGTCGAGATCGAGC
AGCGCGCGGAAGCGCGATATCGCGGCCAGTCATTCCAGCTGATAGTGAACGTACCGCCGGTGGCCATCGCCCGTGGCGAC
GTCGCGGCGATCGAAGACGAATTCCACCGCCAGCACGAGCGTCTCTATGCGCACGCCGATCCGGCGGCGCCGGTGGAATT
GACCGAATTGCGCCTGCGTATTCGCGGCAAGCTGCCGATGCCGTCTGCCGGCGTATCCGGCACGCAGCAAAGCGCACCGG
TCGCAGGCGCCGCCGACGGAGCCCGCAGCGGCGCACGGACGTTGCATATAGCGGGCGCCACGCAGGCCGCGTGCGGCGTC
TATGCCCGCGAGCGACTGCGCACCGGCGACACGCTCGTGGGCGTCGCGATCATCGAACAGCACGACACGACGATTCTGGT
GCCACCACACTACCGGGCGACCGTCGATGCCCACGGCAACATCCTGCTTGAGAAGGAGTCTTGA

Upstream 100 bases:

>100_bases
GAACGAGCCGGGGTTCATCACATTTGCAAATACCACATGCAATGCACGTTCGGAGTTGTCATGCAACAGCAGCCAGGCGG
TGGTGAGAGGCGGAGTCGCA

Downstream 100 bases:

>100_bases
GATGGACGCGGTCCGTACCGCAGTAATGAACAACCGTTTCACGGCAATCGTCGAGGAAGCTTCGGCGATCATGTACCGGA
CGGCGCACACGACCTTCGTC

Product: 5-oxoprolinase (ATP-hydrolyzing)

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 687; Mature: 687

Protein sequence:

>687_residues
MLKIGVDIGGTFTDFAAWREGGEGRVETLKVPSTPPDFAEAFKSGCNEILKRMEPHTHEEIVVVHGTTVATNTVIERNGP
SIALFTTEGFRDILELQRLRLRRAIDLFGNRVDPLIPRELVFEIPERSGSDGRMRTRLDLDAVKHAAHAAKEAGVEGIAI
AFLHSFRNPSHEIAARKAVIEATGLRNVSISSEIWPKIGEYERAIVAVLNTYVKPRMSAYIGEIERWLAARLPNAKLFIM
QSNGGALSADEARELPVHTLLSGPASGVTAAQSLGAALNERCMLTMDMGGTSTDLSLIQDNEPSITGDAEVGDFPLMMPV
TGIEAIGAGGGSVCWIDGGVLRVGPKSAGARPGPACFGHGGTLPTVTDAYLLCGFIDPLHFLGGRMTLDVDAAHAAMQPV
ADALKMDVVSAAQACLTVATSNMVASVLPYLARYGLDPADVTLVVYGGAGSLHGPLLATELGIGRVLVPGMPSVFCAYGG
LVAGLTHDAVKSVQGVTVDGALIQREFAGLEQSAREWLASQDVGVSLREVEIEQRAEARYRGQSFQLIVNVPPVAIARGD
VAAIEDEFHRQHERLYAHADPAAPVELTELRLRIRGKLPMPSAGVSGTQQSAPVAGAADGARSGARTLHIAGATQAACGV
YARERLRTGDTLVGVAIIEQHDTTILVPPHYRATVDAHGNILLEKES

Sequences:

>Translated_687_residues
MLKIGVDIGGTFTDFAAWREGGEGRVETLKVPSTPPDFAEAFKSGCNEILKRMEPHTHEEIVVVHGTTVATNTVIERNGP
SIALFTTEGFRDILELQRLRLRRAIDLFGNRVDPLIPRELVFEIPERSGSDGRMRTRLDLDAVKHAAHAAKEAGVEGIAI
AFLHSFRNPSHEIAARKAVIEATGLRNVSISSEIWPKIGEYERAIVAVLNTYVKPRMSAYIGEIERWLAARLPNAKLFIM
QSNGGALSADEARELPVHTLLSGPASGVTAAQSLGAALNERCMLTMDMGGTSTDLSLIQDNEPSITGDAEVGDFPLMMPV
TGIEAIGAGGGSVCWIDGGVLRVGPKSAGARPGPACFGHGGTLPTVTDAYLLCGFIDPLHFLGGRMTLDVDAAHAAMQPV
ADALKMDVVSAAQACLTVATSNMVASVLPYLARYGLDPADVTLVVYGGAGSLHGPLLATELGIGRVLVPGMPSVFCAYGG
LVAGLTHDAVKSVQGVTVDGALIQREFAGLEQSAREWLASQDVGVSLREVEIEQRAEARYRGQSFQLIVNVPPVAIARGD
VAAIEDEFHRQHERLYAHADPAAPVELTELRLRIRGKLPMPSAGVSGTQQSAPVAGAADGARSGARTLHIAGATQAACGV
YARERLRTGDTLVGVAIIEQHDTTILVPPHYRATVDAHGNILLEKES
>Mature_687_residues
MLKIGVDIGGTFTDFAAWREGGEGRVETLKVPSTPPDFAEAFKSGCNEILKRMEPHTHEEIVVVHGTTVATNTVIERNGP
SIALFTTEGFRDILELQRLRLRRAIDLFGNRVDPLIPRELVFEIPERSGSDGRMRTRLDLDAVKHAAHAAKEAGVEGIAI
AFLHSFRNPSHEIAARKAVIEATGLRNVSISSEIWPKIGEYERAIVAVLNTYVKPRMSAYIGEIERWLAARLPNAKLFIM
QSNGGALSADEARELPVHTLLSGPASGVTAAQSLGAALNERCMLTMDMGGTSTDLSLIQDNEPSITGDAEVGDFPLMMPV
TGIEAIGAGGGSVCWIDGGVLRVGPKSAGARPGPACFGHGGTLPTVTDAYLLCGFIDPLHFLGGRMTLDVDAAHAAMQPV
ADALKMDVVSAAQACLTVATSNMVASVLPYLARYGLDPADVTLVVYGGAGSLHGPLLATELGIGRVLVPGMPSVFCAYGG
LVAGLTHDAVKSVQGVTVDGALIQREFAGLEQSAREWLASQDVGVSLREVEIEQRAEARYRGQSFQLIVNVPPVAIARGD
VAAIEDEFHRQHERLYAHADPAAPVELTELRLRIRGKLPMPSAGVSGTQQSAPVAGAADGARSGARTLHIAGATQAACGV
YARERLRTGDTLVGVAIIEQHDTTILVPPHYRATVDAHGNILLEKES

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=739, Percent_Identity=28.4167794316644, Blast_Score=179, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI133901900, Length=722, Percent_Identity=24.792243767313, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322634, Length=599, Percent_Identity=28.7145242070117, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI45550492, Length=741, Percent_Identity=26.9905533063428, Blast_Score=202, Evalue=8e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 72916; Mature: 72916

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIGVDIGGTFTDFAAWREGGEGRVETLKVPSTPPDFAEAFKSGCNEILKRMEPHTHEE
CEEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
IVVVHGTTVATNTVIERNGPSIALFTTEGFRDILELQRLRLRRAIDLFGNRVDPLIPREL
EEEEECCEEEECEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VFEIPERSGSDGRMRTRLDLDAVKHAAHAAKEAGVEGIAIAFLHSFRNPSHEIAARKAVI
HHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHH
EATGLRNVSISSEIWPKIGEYERAIVAVLNTYVKPRMSAYIGEIERWLAARLPNAKLFIM
HHCCCCCEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
QSNGGALSADEARELPVHTLLSGPASGVTAAQSLGAALNERCMLTMDMGGTSTDLSLIQD
ECCCCCCCCCHHHCCCHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEC
NEPSITGDAEVGDFPLMMPVTGIEAIGAGGGSVCWIDGGVLRVGPKSAGARPGPACFGHG
CCCCCCCCCCCCCCEEEEECCCHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCEECCC
GTLPTVTDAYLLCGFIDPLHFLGGRMTLDVDAAHAAMQPVADALKMDVVSAAQACLTVAT
CCCCHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNMVASVLPYLARYGLDPADVTLVVYGGAGSLHGPLLATELGIGRVLVPGMPSVFCAYGG
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCEEECCCCCHHHHHHHH
LVAGLTHDAVKSVQGVTVDGALIQREFAGLEQSAREWLASQDVGVSLREVEIEQRAEARY
HHHHCCHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RGQSFQLIVNVPPVAIARGDVAAIEDEFHRQHERLYAHADPAAPVELTELRLRIRGKLPM
CCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHEEECCCCCCCCCCEEEHEEEECCCCC
PSAGVSGTQQSAPVAGAADGARSGARTLHIAGATQAACGVYARERLRTGDTLVGVAIIEQ
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEEEE
HDTTILVPPHYRATVDAHGNILLEKES
CCCEEEECCCCEEEEECCCCEEEEECC
>Mature Secondary Structure
MLKIGVDIGGTFTDFAAWREGGEGRVETLKVPSTPPDFAEAFKSGCNEILKRMEPHTHEE
CEEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
IVVVHGTTVATNTVIERNGPSIALFTTEGFRDILELQRLRLRRAIDLFGNRVDPLIPREL
EEEEECCEEEECEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VFEIPERSGSDGRMRTRLDLDAVKHAAHAAKEAGVEGIAIAFLHSFRNPSHEIAARKAVI
HHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHH
EATGLRNVSISSEIWPKIGEYERAIVAVLNTYVKPRMSAYIGEIERWLAARLPNAKLFIM
HHCCCCCEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
QSNGGALSADEARELPVHTLLSGPASGVTAAQSLGAALNERCMLTMDMGGTSTDLSLIQD
ECCCCCCCCCHHHCCCHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEC
NEPSITGDAEVGDFPLMMPVTGIEAIGAGGGSVCWIDGGVLRVGPKSAGARPGPACFGHG
CCCCCCCCCCCCCCEEEEECCCHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCEECCC
GTLPTVTDAYLLCGFIDPLHFLGGRMTLDVDAAHAAMQPVADALKMDVVSAAQACLTVAT
CCCCHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNMVASVLPYLARYGLDPADVTLVVYGGAGSLHGPLLATELGIGRVLVPGMPSVFCAYGG
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCEEECCCCCHHHHHHHH
LVAGLTHDAVKSVQGVTVDGALIQREFAGLEQSAREWLASQDVGVSLREVEIEQRAEARY
HHHHCCHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RGQSFQLIVNVPPVAIARGDVAAIEDEFHRQHERLYAHADPAAPVELTELRLRIRGKLPM
CCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHEEECCCCCCCCCCEEEHEEEECCCCC
PSAGVSGTQQSAPVAGAADGARSGARTLHIAGATQAACGVYARERLRTGDTLVGVAIIEQ
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEEEE
HDTTILVPPHYRATVDAHGNILLEKES
CCCEEEECCCCEEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]