| Definition | Burkholderia xenovorans LB400 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_007951 |
| Length | 4,895,836 |
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The map label for this gene is glnA [H]
Identifier: 91784278
GI number: 91784278
Start: 3234732
End: 3236147
Strand: Reverse
Name: glnA [H]
Synonym: Bxe_A1522
Alternate gene names: 91784278
Gene position: 3236147-3234732 (Counterclockwise)
Preceding gene: 91784279
Following gene: 91784277
Centisome position: 66.1
GC content: 60.73
Gene sequence:
>1416_bases ATGAGTAAATCCGTGGCCGACGTCGTTCAACTCGTCAAAGACGAAGACGTCAAGTTTGTCGACTTCCGTTTCACCGACAC GCGCGGCAAGGAGCAACACGTTTCGGTGCCGGTGTCGGCATTCGATGAAGACAAGTTCGAAAGCGGCCATGCGTTTGACG GTTCGTCGATTGCCGGCTGGAAAGGCATCGAAGCATCGGACATGTTGCTGGTCCCGGACGCGAACACGGCCTTCATCGAC CCGTTCTACGAAGAATCGACCCTCGTGCTGACCTGCGACGTCGTTGAACCGGCGGACGGCAAGGGCTACGAGCGCGACCC GCGCTCGCTGGCAAAGCGCGCCGAAGCGTACCTGAAGAGCACGGGCCTCGGCGACACGGCATTCTTCGGTCCGGAACCCG AATTCTTCATTTTCGACTCGGTCAAGTGGGGCACGGATCAGTCGGGCACGTTCGTCAAGATCGGTTCGGAAGAAGCACCG TGGTCGTCGGCACTCGACTTCGAAGGCGGCAACACCGGCCACCGTCCGGGCACGAAGGGCGGCTATTTCCCGGTCGCGCC GGTCGACACGTTCCAGGACATCCGTTCGGAAATGTGTCTGCTGCTCGAACAGATCGGCATTCCGGTCGAAGTGCATCACC ACGAAGTCGCGGGCCAGGGCCAGAATGAAATCGGCACGAAGTTCTCGACGCTGGTGCAACGCGCCGACTGGCTGCAGCAG ATGAAGTACATCATCCACAACGTCGCGCACACGTACGGCAAGACGGCAACGTTCATGCCGAAGCCGGTGGTCGGCGATAA CGGTTCGGGCATGCACGTTCACCAGTCGATCTGGAAAGACGGCACGAACCTGTTCGCAGGCAACGGTTACGCGGGTCTGT CGGAATTCGCGCTGTTCTACATCGGCGGCATCATCAAGCATGCTCGCGCGCTGAACGCGATCACGAACCCGTCGACCAAC TCGTACAAGCGCCTCGTGCCGCACTTCGAAGCACCGGTCAAGCTGGCTTACTCGGCTCGCAACCGCTCGGCCTCGATCCG TATTCCGCACGTGTCGAACCCGAAGGGCCGCCGTATCGAAACGCGCTTCCCGGATCCGATGGCGAATCCATACCTGTGCT TCTCCGCGCTGATGATGGCGGGTCTGGACGGCGTGCAGAACAAGATTCACCCGGGCGAAGCCGCCGACAAGAACCTGTAC GACCTGCCGCCGGAAGAGGATGCAAAGATCCCGACCGTGTGCGCCGGCCTCGACCAGGCTCTGGACTCGCTCGACGCGGA CCGCGAATTCCTGACGCGCGGTGGCGTGTTCACGGATTCGATGCTCGACGCGTACATCGAACTGAAGACGGGCGAGCTGC AACGCTATCGTCAGTCGGTGCACCCGATCGAATTCGAAATGTACTACTCGCTGTAA
Upstream 100 bases:
>100_bases TATCTTCGTCCTCACCCATTGAGGGGTGGACGTGCCGGGACGGGTCAGCTAGATTGGGCGGCGGCTGAATCCGCCGAATT CGTTAATCAGGAGATAGGTT
Downstream 100 bases:
>100_bases GCGGCAATGGCGCTTCGCCCTTTACACGGCGAAGTGCTTTGCCCGACGCTCGCGATCGGTCACGAAGGGACGGCGGCGCC GTCCCTTTTTCGTTAAGGCC
Product: L-glutamine synthetase
Products: NA
Alternate protein names: Glutamate--ammonia ligase [H]
Number of amino acids: Translated: 471; Mature: 470
Protein sequence:
>471_residues MSKSVADVVQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGWKGIEASDMLLVPDANTAFID PFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKSTGLGDTAFFGPEPEFFIFDSVKWGTDQSGTFVKIGSEEAP WSSALDFEGGNTGHRPGTKGGYFPVAPVDTFQDIRSEMCLLLEQIGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWLQQ MKYIIHNVAHTYGKTATFMPKPVVGDNGSGMHVHQSIWKDGTNLFAGNGYAGLSEFALFYIGGIIKHARALNAITNPSTN SYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIETRFPDPMANPYLCFSALMMAGLDGVQNKIHPGEAADKNLY DLPPEEDAKIPTVCAGLDQALDSLDADREFLTRGGVFTDSMLDAYIELKTGELQRYRQSVHPIEFEMYYSL
Sequences:
>Translated_471_residues MSKSVADVVQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGWKGIEASDMLLVPDANTAFID PFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKSTGLGDTAFFGPEPEFFIFDSVKWGTDQSGTFVKIGSEEAP WSSALDFEGGNTGHRPGTKGGYFPVAPVDTFQDIRSEMCLLLEQIGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWLQQ MKYIIHNVAHTYGKTATFMPKPVVGDNGSGMHVHQSIWKDGTNLFAGNGYAGLSEFALFYIGGIIKHARALNAITNPSTN SYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIETRFPDPMANPYLCFSALMMAGLDGVQNKIHPGEAADKNLY DLPPEEDAKIPTVCAGLDQALDSLDADREFLTRGGVFTDSMLDAYIELKTGELQRYRQSVHPIEFEMYYSL >Mature_470_residues SKSVADVVQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGWKGIEASDMLLVPDANTAFIDP FYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKSTGLGDTAFFGPEPEFFIFDSVKWGTDQSGTFVKIGSEEAPW SSALDFEGGNTGHRPGTKGGYFPVAPVDTFQDIRSEMCLLLEQIGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWLQQM KYIIHNVAHTYGKTATFMPKPVVGDNGSGMHVHQSIWKDGTNLFAGNGYAGLSEFALFYIGGIIKHARALNAITNPSTNS YKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIETRFPDPMANPYLCFSALMMAGLDGVQNKIHPGEAADKNLYD LPPEEDAKIPTVCAGLDQALDSLDADREFLTRGGVFTDSMLDAYIELKTGELQRYRQSVHPIEFEMYYSL
Specific function: Unknown
COG id: COG0174
COG function: function code E; Glutamine synthetase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glutamine synthetase family [H]
Homologues:
Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=64.7435897435898, Blast_Score=670, Evalue=0.0, Organism=Escherichia coli, GI87081870, Length=399, Percent_Identity=24.812030075188, Blast_Score=91, Evalue=2e-19,
Paralogues:
None
Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR008147 - InterPro: IPR014746 - InterPro: IPR008146 - InterPro: IPR004809 - InterPro: IPR001637 [H]
Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]
EC number: =6.3.1.2 [H]
Molecular weight: Translated: 52028; Mature: 51897
Theoretical pI: Translated: 5.04; Mature: 5.04
Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 3.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSKSVADVVQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGW CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCC KGIEASDMLLVPDANTAFIDPFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKS CCCCCCCEEEECCCCCEEECCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH TGLGDTAFFGPEPEFFIFDSVKWGTDQSGTFVKIGSEEAPWSSALDFEGGNTGHRPGTKG CCCCCCCCCCCCCCEEEEECEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC GYFPVAPVDTFQDIRSEMCLLLEQIGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWLQQ CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHH MKYIIHNVAHTYGKTATFMPKPVVGDNGSGMHVHQSIWKDGTNLFAGNGYAGLSEFALFY HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHH IGGIIKHARALNAITNPSTNSYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIE HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCEEE TRFPDPMANPYLCFSALMMAGLDGVQNKIHPGEAADKNLYDLPPEEDAKIPTVCAGLDQA CCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH LDSLDADREFLTRGGVFTDSMLDAYIELKTGELQRYRQSVHPIEFEMYYSL HHHHCCCHHHHHCCCCCHHHHHHHHHEECCHHHHHHHHHCCCEEEEEEECC >Mature Secondary Structure SKSVADVVQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGW CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCC KGIEASDMLLVPDANTAFIDPFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKS CCCCCCCEEEECCCCCEEECCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH TGLGDTAFFGPEPEFFIFDSVKWGTDQSGTFVKIGSEEAPWSSALDFEGGNTGHRPGTKG CCCCCCCCCCCCCCEEEEECEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC GYFPVAPVDTFQDIRSEMCLLLEQIGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWLQQ CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHH MKYIIHNVAHTYGKTATFMPKPVVGDNGSGMHVHQSIWKDGTNLFAGNGYAGLSEFALFY HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHH IGGIIKHARALNAITNPSTNSYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIE HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCEEE TRFPDPMANPYLCFSALMMAGLDGVQNKIHPGEAADKNLYDLPPEEDAKIPTVCAGLDQA CCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH LDSLDADREFLTRGGVFTDSMLDAYIELKTGELQRYRQSVHPIEFEMYYSL HHHHCCCHHHHHCCCCCHHHHHHHHHEECCHHHHHHHHHCCCEEEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 1969923 [H]