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The map label for this gene is iscA [H]

Identifier: 91784246

GI number: 91784246

Start: 3193948

End: 3194283

Strand: Reverse

Name: iscA [H]

Synonym: Bxe_A1555

Alternate gene names: 91784246

Gene position: 3194283-3193948 (Counterclockwise)

Preceding gene: 91784247

Following gene: 91784245

Centisome position: 65.24

GC content: 56.85

Gene sequence:

>336_bases
ATGAGAAACGCTATGGCAATTACGTTGACCGAAAAGGCAGCACAGCACGTCCAGAAGTATCTGAACCGTCGCGGTAAAGG
CGTCGGCCTGCGCGTCGGCGTGCGCACCACCGGTTGCTCTGGCTTGGCCTACAAGCTCGAGTACGTGGATGAACTCGCGC
CCGAAGACGAAGTCTTCGAATGCAACGGCGTGAAGATCATTGTCGACCCGAAGAGCCTCGCCTATATCGACGGCACCGAA
CTCGACTTTGCGCGCGAAGGGTTGAACGAAGGCTTCAAGTTCAACAACCCGAACGTGAAGGACGAATGCGGTTGCGGCGA
ATCGTTCCGCGTATAG

Upstream 100 bases:

>100_bases
CGGCGCGAATTCAGACCAGCCGTCCGGCACGAGCTATATTTGATGCAGGCAGGGTAGCAGCGCGTGACCAGGCAGACGAA
CGGCCGGCGCGCACTGCGCA

Downstream 100 bases:

>100_bases
ATCCGGATATTTCCGCGTGCAGCGGATCAAAGGCGGCGCGGGCCGCCTTTTTAGTTTGAACCGCGCATTCGCCCATGCCG
CTATGCTCCGCTTTGTCGAG

Product: Iron-sulphur cluster assembly protein IscA

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 111; Mature: 111

Protein sequence:

>111_residues
MRNAMAITLTEKAAQHVQKYLNRRGKGVGLRVGVRTTGCSGLAYKLEYVDELAPEDEVFECNGVKIIVDPKSLAYIDGTE
LDFAREGLNEGFKFNNPNVKDECGCGESFRV

Sequences:

>Translated_111_residues
MRNAMAITLTEKAAQHVQKYLNRRGKGVGLRVGVRTTGCSGLAYKLEYVDELAPEDEVFECNGVKIIVDPKSLAYIDGTE
LDFAREGLNEGFKFNNPNVKDECGCGESFRV
>Mature_111_residues
MRNAMAITLTEKAAQHVQKYLNRRGKGVGLRVGVRTTGCSGLAYKLEYVDELAPEDEVFECNGVKIIVDPKSLAYIDGTE
LDFAREGLNEGFKFNNPNVKDECGCGESFRV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=42.4528301886792, Blast_Score=98, Evalue=1e-21,
Organism=Homo sapiens, GI160420328, Length=83, Percent_Identity=39.7590361445783, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=69.1588785046729, Blast_Score=159, Evalue=3e-41,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=48.5714285714286, Blast_Score=112, Evalue=4e-27,
Organism=Escherichia coli, GI1786351, Length=111, Percent_Identity=37.8378378378378, Blast_Score=72, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=39.622641509434, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=39.622641509434, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=46.6666666666667, Blast_Score=96, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24642263, Length=106, Percent_Identity=44.3396226415094, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 12277; Mature: 12277

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNAMAITLTEKAAQHVQKYLNRRGKGVGLRVGVRTTGCSGLAYKLEYVDELAPEDEVFE
CCCCEEEEECHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEHHHHHHHHCCCCCEEE
CNGVKIIVDPKSLAYIDGTELDFAREGLNEGFKFNNPNVKDECGCGESFRV
ECCEEEEECCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRNAMAITLTEKAAQHVQKYLNRRGKGVGLRVGVRTTGCSGLAYKLEYVDELAPEDEVFE
CCCCEEEEECHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEHHHHHHHHCCCCCEEE
CNGVKIIVDPKSLAYIDGTELDFAREGLNEGFKFNNPNVKDECGCGESFRV
ECCEEEEECCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA