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The map label for this gene is rlmN [H]

Identifier: 91784209

GI number: 91784209

Start: 3154527

End: 3155678

Strand: Reverse

Name: rlmN [H]

Synonym: Bxe_A1592

Alternate gene names: 91784209

Gene position: 3155678-3154527 (Counterclockwise)

Preceding gene: 91784210

Following gene: 91784208

Centisome position: 64.46

GC content: 62.41

Gene sequence:

>1152_bases
ATGACGAGCAGTCCCACCGTCAACCTTCTCGACCTCGACGCCCAAGGGCTTGTCGCCTACTGCGACAGCCTGGGCGAGAA
GCCGTTTCGCGCCAGGCAATTGCAGCGCTGGATTCACCAGTACAACGCCGCCGACTTCGACGGCATGACCGATCTGGCCA
AGTCCTTGCGCGAAAAGCTCAAGGGGCGCGCCACGATCTCGATGCCGGGCATCGTCAGCGACCATATTTCGACCGACGGC
ACACGCAAGTGGCTGATCGACGTCGGCAACAGCAACGCGGTCGAAACCGTCTACATTCCCGAGGAAACGCGCGGCACGCT
ATGTGTGTCGTCGCAGGCCGGGTGTGCGGTCAATTGCCGTTTCTGTTCGACCGGCAAACAGGGTTTCTCCCGCAATCTCA
CTACCGGCGAAATCATCGGCCAGTTGCGTATGGCCGAGTTCGCGCTGCGCGCGTCGCGCGGCGTGGACGGCGGCCGCGCC
ACGGGTGGTGACGGCAAGGGCGAGCGCGTCGTCACGAATGTCGTGATGATGGGCATGGGCGAGCCGCTGCTCAACTACGA
CGCGGTCGTGCCGGCCATGCGCCTGATGCTCGACGACAATGCCTACGGTTTGTCGCGCCGCCGTGTCACGCTGTCCACTT
CGGGTGTCGTGCCCATGATGGACCGGCTCGGCGCTGATCTGCCCGTGGCGCTCGCGGTATCCTTGCATGCCCCCAGCGAT
CCATTGCGTGACATGCTGGTGCCGTTGAACAAAAAGTACCCGCTGCGCGAATTGATGGCGGCTTGCCAGCGCTATCTGAA
GGTCGCGCCGCGCGACTTCATTACGTTTGAATATTGCATGCTCGACGGCGTGAACGACAGCGAAGCGCAAGCGCGCGAGT
TGCTGGCGGTTACGCGCGACGTGCCGTGCAAGTTCAACCTGATTCCGTTCAATCCGTTCCCCGAATCGGGCCTCATCCGC
TCGAAACCGGAACAGATCAAGCGGTTTGCACAAGTGTTGATGGACGCGGGCGTCATCACCACGGTGCGTAAAACACGCGG
CGATGATATCGACGCTGCCTGCGGTCAGTTGGCCGGTGCGGTGAAAGACCGCACCCGCCTCGCTGAGCGCACCGGGAAGG
CAGCGAAGATTATCGAGGTTCGTGCCGTGTAA

Upstream 100 bases:

>100_bases
AAGTAGTAAGGTAAAAGCAGCAGGAATGGGCGCGAACGGCATGGCGTTCATGCAGCTTGTTGCATGAACGTAGTCTCGCA
CTGAAATGGCAGGATTCGAT

Downstream 100 bases:

>100_bases
TCGTGCGGGGCGGCAGGGTGGCGGCGTTCGGAGAAAGCTCTGCAAACGCGCGAAAACCTGCGGTACCAGTGTCGATTGAA
AAGAAAGTTTGCAGAAGCGA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTSSPTVNLLDLDAQGLVAYCDSLGEKPFRARQLQRWIHQYNAADFDGMTDLAKSLREKLKGRATISMPGIVSDHISTDG
TRKWLIDVGNSNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLTTGEIIGQLRMAEFALRASRGVDGGRA
TGGDGKGERVVTNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGADLPVALAVSLHAPSD
PLRDMLVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDSEAQARELLAVTRDVPCKFNLIPFNPFPESGLIR
SKPEQIKRFAQVLMDAGVITTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGKAAKIIEVRAV

Sequences:

>Translated_383_residues
MTSSPTVNLLDLDAQGLVAYCDSLGEKPFRARQLQRWIHQYNAADFDGMTDLAKSLREKLKGRATISMPGIVSDHISTDG
TRKWLIDVGNSNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLTTGEIIGQLRMAEFALRASRGVDGGRA
TGGDGKGERVVTNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGADLPVALAVSLHAPSD
PLRDMLVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDSEAQARELLAVTRDVPCKFNLIPFNPFPESGLIR
SKPEQIKRFAQVLMDAGVITTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGKAAKIIEVRAV
>Mature_382_residues
TSSPTVNLLDLDAQGLVAYCDSLGEKPFRARQLQRWIHQYNAADFDGMTDLAKSLREKLKGRATISMPGIVSDHISTDGT
RKWLIDVGNSNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLTTGEIIGQLRMAEFALRASRGVDGGRAT
GGDGKGERVVTNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGADLPVALAVSLHAPSDP
LRDMLVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDSEAQARELLAVTRDVPCKFNLIPFNPFPESGLIRS
KPEQIKRFAQVLMDAGVITTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGKAAKIIEVRAV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=384, Percent_Identity=53.90625, Blast_Score=402, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41821; Mature: 41690

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSPTVNLLDLDAQGLVAYCDSLGEKPFRARQLQRWIHQYNAADFDGMTDLAKSLREKL
CCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRATISMPGIVSDHISTDGTRKWLIDVGNSNAVETVYIPEETRGTLCVSSQAGCAVNCR
CCCEEEECCCCHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLTTGEIIGQLRMAEFALRASRGVDGGRATGGDGKGERVVTNVVMMGMG
EECCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCC
EPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGADLPVALAVSLHAPSD
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEECCCCC
PLRDMLVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDSEAQARELLAVTRD
CHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHCC
VPCKFNLIPFNPFPESGLIRSKPEQIKRFAQVLMDAGVITTVRKTRGDDIDAACGQLAGA
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHH
VKDRTRLAERTGKAAKIIEVRAV
HHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure 
TSSPTVNLLDLDAQGLVAYCDSLGEKPFRARQLQRWIHQYNAADFDGMTDLAKSLREKL
CCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRATISMPGIVSDHISTDGTRKWLIDVGNSNAVETVYIPEETRGTLCVSSQAGCAVNCR
CCCEEEECCCCHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLTTGEIIGQLRMAEFALRASRGVDGGRATGGDGKGERVVTNVVMMGMG
EECCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCC
EPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGADLPVALAVSLHAPSD
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEECCCCC
PLRDMLVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDSEAQARELLAVTRD
CHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHCC
VPCKFNLIPFNPFPESGLIRSKPEQIKRFAQVLMDAGVITTVRKTRGDDIDAACGQLAGA
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHH
VKDRTRLAERTGKAAKIIEVRAV
HHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA