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The map label for this gene is iciA [H]

Identifier: 91784058

GI number: 91784058

Start: 2980137

End: 2981090

Strand: Reverse

Name: iciA [H]

Synonym: Bxe_A1745

Alternate gene names: 91784058

Gene position: 2981090-2980137 (Counterclockwise)

Preceding gene: 91784059

Following gene: 91784054

Centisome position: 60.89

GC content: 56.92

Gene sequence:

>954_bases
ATGAATTTTGATCGACAACAGTTGGAGGCGTTTTCCGCCATTGTGGAGACGCGGCATTTCGGACGTGCCGCCAAGTTGTT
GAGTATCACTCGTGGTGCGGTGTCTCTTCGGCTCAAGGCACTTGAGGAGGAGTTCGGTACTCCACTGGTTGTGCGAGATG
GAAATGTCCCCACGCCAGCAGGCGAGGTCCTGCTGAGGCATATCCGTGTGCTCAAGATGCTCGAGGCGGAAACGCAGCAG
CAGATTAAACCAACGGACGGCATGCACCCACGCGTGGCTATCGCTGTGAATGCGGATTCCCTCGCAACGTGGTTCGAGGC
TGTCGCCTGGGAAATTGCGGAAAGAAACGTAGCTCTCGAACTGATCGTTGACGATCAGGATTACACCCTACCACTACTGA
CCAAGGGAGAGGCGATGGGCTGTGTTTCGACCTCCAGCAATCCCCCGACGGGTTTTCTCGCTGAACCCATTGGCATGATG
GAGTATGAGTGTGTCGCGAACGTGGAGTTCGCCGCAAAGTTCTTCCCGCAAGGCCTGGGGTTGCATGGCGTCCTTGCCGC
GCCTGCGGTGCTCTACAACCGGAAGGATGGATTGCACACGCTGTTTCTCGAGCGACTTCTGGACCTTGAGGTACGGAACT
ACGCGACGCATTACTTTCCGTCGCCGTCGGCATTGCTGGCTGCGATCGAGAAGGGGATAGGGTACGGACTCGTTCCCTGT
CTGCAAGCCAGCTCTCTGATCGAAGCGGGCAAGCTGGTGCGCCTCGCGCCTCAGCATAAGGTGGAAGTCAACCTCTATTG
GCATCATTGGGAAACTGCCCCGTCGAATGCCCAGGCGATTAGCGAACTTGTGGTGCGTCATGCGCACCGGGCGCTCATTC
AGGCGGGTGATCATGCTATGTCGTTCAGCGATGGTGATGAGTCTGTTGACACTGATCATCCCAGTGAGCGTTGA

Upstream 100 bases:

>100_bases
TCCGCACCGCGTTGGCCAAACGCCTCGCCGTGCTGGAGCCGCAGAAGACCGTCGCGCTTTCCGTGATGGAGCGCGCATAA
CGAGCGCGCAGAATCTGGTC

Downstream 100 bases:

>100_bases
TGCGGTTCTGCCGGAGTTAGGCACTGAAATCTTCTCTGGCTGATTGGGGCGCGAAATCGCAGGGATGCAGCGAACGCAAG
GCCATGCCGGTATTAGCGGC

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MNFDRQQLEAFSAIVETRHFGRAAKLLSITRGAVSLRLKALEEEFGTPLVVRDGNVPTPAGEVLLRHIRVLKMLEAETQQ
QIKPTDGMHPRVAIAVNADSLATWFEAVAWEIAERNVALELIVDDQDYTLPLLTKGEAMGCVSTSSNPPTGFLAEPIGMM
EYECVANVEFAAKFFPQGLGLHGVLAAPAVLYNRKDGLHTLFLERLLDLEVRNYATHYFPSPSALLAAIEKGIGYGLVPC
LQASSLIEAGKLVRLAPQHKVEVNLYWHHWETAPSNAQAISELVVRHAHRALIQAGDHAMSFSDGDESVDTDHPSER

Sequences:

>Translated_317_residues
MNFDRQQLEAFSAIVETRHFGRAAKLLSITRGAVSLRLKALEEEFGTPLVVRDGNVPTPAGEVLLRHIRVLKMLEAETQQ
QIKPTDGMHPRVAIAVNADSLATWFEAVAWEIAERNVALELIVDDQDYTLPLLTKGEAMGCVSTSSNPPTGFLAEPIGMM
EYECVANVEFAAKFFPQGLGLHGVLAAPAVLYNRKDGLHTLFLERLLDLEVRNYATHYFPSPSALLAAIEKGIGYGLVPC
LQASSLIEAGKLVRLAPQHKVEVNLYWHHWETAPSNAQAISELVVRHAHRALIQAGDHAMSFSDGDESVDTDHPSER
>Mature_317_residues
MNFDRQQLEAFSAIVETRHFGRAAKLLSITRGAVSLRLKALEEEFGTPLVVRDGNVPTPAGEVLLRHIRVLKMLEAETQQ
QIKPTDGMHPRVAIAVNADSLATWFEAVAWEIAERNVALELIVDDQDYTLPLLTKGEAMGCVSTSSNPPTGFLAEPIGMM
EYECVANVEFAAKFFPQGLGLHGVLAAPAVLYNRKDGLHTLFLERLLDLEVRNYATHYFPSPSALLAAIEKGIGYGLVPC
LQASSLIEAGKLVRLAPQHKVEVNLYWHHWETAPSNAQAISELVVRHAHRALIQAGDHAMSFSDGDESVDTDHPSER

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=295, Percent_Identity=33.2203389830508, Blast_Score=150, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34877; Mature: 34877

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFDRQQLEAFSAIVETRHFGRAAKLLSITRGAVSLRLKALEEEFGTPLVVRDGNVPTPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCH
GEVLLRHIRVLKMLEAETQQQIKPTDGMHPRVAIAVNADSLATWFEAVAWEIAERNVALE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEE
LIVDDQDYTLPLLTKGEAMGCVSTSSNPPTGFLAEPIGMMEYECVANVEFAAKFFPQGLG
EEECCCCCEEEEEECCCCCEEEECCCCCCCCCHHCCCCCHHHHHHCCHHHHHHHCCCCCC
LHGVLAAPAVLYNRKDGLHTLFLERLLDLEVRNYATHYFPSPSALLAAIEKGIGYGLVPC
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHH
LQASSLIEAGKLVRLAPQHKVEVNLYWHHWETAPSNAQAISELVVRHAHRALIQAGDHAM
HHHHHHHHCCCEEEECCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
SFSDGDESVDTDHPSER
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNFDRQQLEAFSAIVETRHFGRAAKLLSITRGAVSLRLKALEEEFGTPLVVRDGNVPTPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCH
GEVLLRHIRVLKMLEAETQQQIKPTDGMHPRVAIAVNADSLATWFEAVAWEIAERNVALE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEE
LIVDDQDYTLPLLTKGEAMGCVSTSSNPPTGFLAEPIGMMEYECVANVEFAAKFFPQGLG
EEECCCCCEEEEEECCCCCEEEECCCCCCCCCHHCCCCCHHHHHHCCHHHHHHHCCCCCC
LHGVLAAPAVLYNRKDGLHTLFLERLLDLEVRNYATHYFPSPSALLAAIEKGIGYGLVPC
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHH
LQASSLIEAGKLVRLAPQHKVEVNLYWHHWETAPSNAQAISELVVRHAHRALIQAGDHAM
HHHHHHHHCCCEEEECCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
SFSDGDESVDTDHPSER
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9286976 [H]