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The map label for this gene is virB8 [H]

Identifier: 91784044

GI number: 91784044

Start: 2967900

End: 2968619

Strand: Reverse

Name: virB8 [H]

Synonym: Bxe_A1759

Alternate gene names: 91784044

Gene position: 2968619-2967900 (Counterclockwise)

Preceding gene: 91784045

Following gene: 91784043

Centisome position: 60.64

GC content: 65.42

Gene sequence:

>720_bases
ATGAGCGCGCAGCACAACTACGAGCGTGTGCTCGACATCGAGGCGTCGCTGACCTACCTGCAGGAACGTTCCGAGCGGCG
CGCGTGGCATGTCGCCTATGCCGCGGTCGGTGTGGCCGTGCTGAGTGTTGCCGCTCTCGCGGTGATGGTGCCGTTCTACC
GCGTGGTGCCCTTGCCGATCGAGGTCGATCGCCTGACGGGCGAGTCCCAGGTCATCGATGTACTCGATGCACGCCATGTG
CATACGAAGGAGATCCAGGACAAGCACTGGGTCGAGACCTATGTGCGCGAGCGCGAACGCTACGACTGGGGCCTGCTGCA
GATGGACTACGATAGCGTGCTCGCGATGAGCGATGACGTGGTCGCACGCGACTACCGCGGCATCTATAGCGGCTCCGATG
CACTCGACCGGCAACTCGGGGCGGGCACCGAGCGGCGCGTGCGGATTCTCTCGGTCACGCTGCCGCCCGATGAACCGGGG
CACGCGGTCGTGCATGTCGAGCGCACGACGTTCAGGAACGGCCAGGCCGATGCCGAGCCGACGCAGCGTTTCGTGATCAC
GCTGGCCTATACCTACAGGCCGCCTGTGTTTACGCGCGAGAGCGTGGCCATCGCCAATCCGTTCGGCTTCAAGGTCACGG
CCTACAGCCGCGATCCCGAATATACGCCGCCGGCATCGGCTGCGGCATCCGCGACCACGACTGCGGGAGGCACGCCATGA

Upstream 100 bases:

>100_bases
CCGACGGCCTGCATCGCGTGCCGGTCAACCGGGCGCCTCCTGTGTCGGAAAGCGAGCCTGTTCCTGACAGGCCAGCTATA
CCGGATAGCGGAGACCGGTC

Downstream 100 bases:

>100_bases
AAACGCTTCTTTCCGCGCGTCTGTTCACGATGGCGCTCGTGGGCCTGCTGTTTTCGGGCATGGTCCACGCGGTCGACCTG
CCGGGCTGGAACGACGATTC

Product: putative type IV secretion system protein VirB8/TraJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSAQHNYERVLDIEASLTYLQERSERRAWHVAYAAVGVAVLSVAALAVMVPFYRVVPLPIEVDRLTGESQVIDVLDARHV
HTKEIQDKHWVETYVRERERYDWGLLQMDYDSVLAMSDDVVARDYRGIYSGSDALDRQLGAGTERRVRILSVTLPPDEPG
HAVVHVERTTFRNGQADAEPTQRFVITLAYTYRPPVFTRESVAIANPFGFKVTAYSRDPEYTPPASAAASATTTAGGTP

Sequences:

>Translated_239_residues
MSAQHNYERVLDIEASLTYLQERSERRAWHVAYAAVGVAVLSVAALAVMVPFYRVVPLPIEVDRLTGESQVIDVLDARHV
HTKEIQDKHWVETYVRERERYDWGLLQMDYDSVLAMSDDVVARDYRGIYSGSDALDRQLGAGTERRVRILSVTLPPDEPG
HAVVHVERTTFRNGQADAEPTQRFVITLAYTYRPPVFTRESVAIANPFGFKVTAYSRDPEYTPPASAAASATTTAGGTP
>Mature_238_residues
SAQHNYERVLDIEASLTYLQERSERRAWHVAYAAVGVAVLSVAALAVMVPFYRVVPLPIEVDRLTGESQVIDVLDARHVH
TKEIQDKHWVETYVRERERYDWGLLQMDYDSVLAMSDDVVARDYRGIYSGSDALDRQLGAGTERRVRILSVTLPPDEPGH
AVVHVERTTFRNGQADAEPTQRFVITLAYTYRPPVFTRESVAIANPFGFKVTAYSRDPEYTPPASAAASATTTAGGTP

Specific function: The virB operon is essential for intracellular survival and is not involved in the invasion process. Constitutes a major determinant of virulence in mice [H]

COG id: COG3736

COG function: function code U; Type IV secretory pathway, component VirB8

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the virB8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007430 [H]

Pfam domain/function: PF04335 VirB8 [H]

EC number: NA

Molecular weight: Translated: 26726; Mature: 26595

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQHNYERVLDIEASLTYLQERSERRAWHVAYAAVGVAVLSVAALAVMVPFYRVVPLPI
CCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EVDRLTGESQVIDVLDARHVHTKEIQDKHWVETYVRERERYDWGLLQMDYDSVLAMSDDV
EEECCCCCHHHHHHHHCCCCHHHHHCHHHHHHHHHHHHHCCCCCEEEECCHHHEECCCCH
VARDYRGIYSGSDALDRQLGAGTERRVRILSVTLPPDEPGHAVVHVERTTFRNGQADAEP
HHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEEEEEECCCCCCCCCC
TQRFVITLAYTYRPPVFTRESVAIANPFGFKVTAYSRDPEYTPPASAAASATTTAGGTP
CEEEEEEEEEEECCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCHHCCCCCCCCCCC
>Mature Secondary Structure 
SAQHNYERVLDIEASLTYLQERSERRAWHVAYAAVGVAVLSVAALAVMVPFYRVVPLPI
CCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EVDRLTGESQVIDVLDARHVHTKEIQDKHWVETYVRERERYDWGLLQMDYDSVLAMSDDV
EEECCCCCHHHHHHHHCCCCHHHHHCHHHHHHHHHHHHHCCCCCEEEECCHHHEECCCCH
VARDYRGIYSGSDALDRQLGAGTERRVRILSVTLPPDEPGHAVVHVERTTFRNGQADAEP
HHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEEEEEECCCCCCCCCC
TQRFVITLAYTYRPPVFTRESVAIANPFGFKVTAYSRDPEYTPPASAAASATTTAGGTP
CEEEEEEEEEEECCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10940027 [H]