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The map label for this gene is liaS [H]

Identifier: 91783994

GI number: 91783994

Start: 2906217

End: 2907596

Strand: Reverse

Name: liaS [H]

Synonym: Bxe_A1814

Alternate gene names: 91783994

Gene position: 2907596-2906217 (Counterclockwise)

Preceding gene: 91783995

Following gene: 91783993

Centisome position: 59.39

GC content: 63.7

Gene sequence:

>1380_bases
ATGGCCGGCCGCTGGCGTAACAGTCTCTTCTGGCGCTTTCTATGCGCGATGATGCTGGCGTCATGCGCCGGCGTGACCGT
CACAGTCGCGGGGAATGCGATACTCGACAACTGGCGTGTGTCGGAAGCGTTTTCGTCGCCGGCACTCACGCGCGCATTCG
AGCGTGAACTCGCGCCGAATGCGGCCCTTCTGGCGCAAGCGCGCCAATCGCCAGAGATTTGCCGACTGGTCCTGCACGAG
TTGTCCGCCAGAGTGCTGACCGACCGGCTGCCGGCTATCAAGGGCCCGAATCGCGTGAGTCGCGCGCTCGATGGTGGCCG
GGTGTTCGTCCGCTATCAATGGCCCGATGGGGTAACGTGCCGTTTCCCCAATGGAGTTGGCAGCGCCGCATCTTCTGATG
CGCAGGGCAACGGCAAGACCGTCACGCTGACGGTACGTTCGCCCGTGGAACCCGGCGCGACCCTGACGGTCGGCATGTTC
GTGCTGTCGCCGCTCGCATCCCTGCTGCATAGTGACGATGTGTCATGGACGGTCATCGGGCTATACGTACTCGTCATCAA
TTTCTGTTCCGTGCTGGTGCTGGTGCCGTTGCTGGTCAAACGGATCCGCAAGGCCGAACGCGCGGCATCCGCGTGGACGG
ACGGCGATCTGGCGGCGCGTATCGAAGACGGCGGCAAGGACGAATTCGGCCGGCTCACCACTAGTTTCGACGAGATGGCC
GATGCCCTGTCTTCCATGATCGAGATGAAACTGGCGCTGGCGGCTTCACAGGAACGTAACCGCCTCGCGCGCGACCTGCA
CGATACGGCGAAGCAGCGCGCTTTCGCGCTGGGACTGCAGCTCACGGCGTTGAAAAAGCATGGCGCGGCGAACGTGGATT
CGGCGAACCTTACGCGCGCGTCTCTGGCGCTGGTGTCGCATCTTCAGCAGGACCTGGCCGATGTCATCCGCCGGCTGTCG
GCGCCGACGATCGCGGAAATCGGCTTGCGGCGCGCGTTGAGCGACAGCATCGACGCGTTGCTCGCCGGTGCCCGCGCGCG
CTGGACCCTGCACCTGAGCCACGACGACGAACGCGCGCTCGAAGTCGTGCCGGACATTGCGCGTCAGTTGCTGCTGATCT
CGATCGAAGCCTGTGCGAATGTGCTGAAGCATGCCGCCGCGACCCATGTGGAGCTGGCGTTCAGCCGGCTCGACGATACG
TACACCTTGAAGATCGCCGACGACGGCCAGGGCTTTGACGCACGGCGCGTCGAAGCGTTGGGCATGGGACTGTCCAACAT
GCGCCTGAGGGCCAACAGTTTGCCGCACGGAGAACTTCACCTGGCGAGCAGCCCGGGAAAAGGCGCCGAAATCACTGTCT
CGTTCCAATTAACGCTTTGA

Upstream 100 bases:

>100_bases
GCAGCCTATACGTTCTGATGCAGCGGTCTTCCCATTTCTCTTTCACGGACGCTAATTCCGGGTGAACGACACGGCGCAGC
GCACATTTTCTCCCATCGTT

Downstream 100 bases:

>100_bases
GGGTTCGATGAAATTCACGGTCTTGCTGGTCGACGATCACGCCGTTGTGCGGCAGGGTCTGGTGAGTATCCTGACGATGA
CCGACGACTTCACACTGGTC

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MAGRWRNSLFWRFLCAMMLASCAGVTVTVAGNAILDNWRVSEAFSSPALTRAFERELAPNAALLAQARQSPEICRLVLHE
LSARVLTDRLPAIKGPNRVSRALDGGRVFVRYQWPDGVTCRFPNGVGSAASSDAQGNGKTVTLTVRSPVEPGATLTVGMF
VLSPLASLLHSDDVSWTVIGLYVLVINFCSVLVLVPLLVKRIRKAERAASAWTDGDLAARIEDGGKDEFGRLTTSFDEMA
DALSSMIEMKLALAASQERNRLARDLHDTAKQRAFALGLQLTALKKHGAANVDSANLTRASLALVSHLQQDLADVIRRLS
APTIAEIGLRRALSDSIDALLAGARARWTLHLSHDDERALEVVPDIARQLLLISIEACANVLKHAAATHVELAFSRLDDT
YTLKIADDGQGFDARRVEALGMGLSNMRLRANSLPHGELHLASSPGKGAEITVSFQLTL

Sequences:

>Translated_459_residues
MAGRWRNSLFWRFLCAMMLASCAGVTVTVAGNAILDNWRVSEAFSSPALTRAFERELAPNAALLAQARQSPEICRLVLHE
LSARVLTDRLPAIKGPNRVSRALDGGRVFVRYQWPDGVTCRFPNGVGSAASSDAQGNGKTVTLTVRSPVEPGATLTVGMF
VLSPLASLLHSDDVSWTVIGLYVLVINFCSVLVLVPLLVKRIRKAERAASAWTDGDLAARIEDGGKDEFGRLTTSFDEMA
DALSSMIEMKLALAASQERNRLARDLHDTAKQRAFALGLQLTALKKHGAANVDSANLTRASLALVSHLQQDLADVIRRLS
APTIAEIGLRRALSDSIDALLAGARARWTLHLSHDDERALEVVPDIARQLLLISIEACANVLKHAAATHVELAFSRLDDT
YTLKIADDGQGFDARRVEALGMGLSNMRLRANSLPHGELHLASSPGKGAEITVSFQLTL
>Mature_458_residues
AGRWRNSLFWRFLCAMMLASCAGVTVTVAGNAILDNWRVSEAFSSPALTRAFERELAPNAALLAQARQSPEICRLVLHEL
SARVLTDRLPAIKGPNRVSRALDGGRVFVRYQWPDGVTCRFPNGVGSAASSDAQGNGKTVTLTVRSPVEPGATLTVGMFV
LSPLASLLHSDDVSWTVIGLYVLVINFCSVLVLVPLLVKRIRKAERAASAWTDGDLAARIEDGGKDEFGRLTTSFDEMAD
ALSSMIEMKLALAASQERNRLARDLHDTAKQRAFALGLQLTALKKHGAANVDSANLTRASLALVSHLQQDLADVIRRLSA
PTIAEIGLRRALSDSIDALLAGARARWTLHLSHDDERALEVVPDIARQLLLISIEACANVLKHAAATHVELAFSRLDDTY
TLKIADDGQGFDARRVEALGMGLSNMRLRANSLPHGELHLASSPGKGAEITVSFQLTL

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=221, Percent_Identity=28.5067873303167, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49593; Mature: 49461

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRWRNSLFWRFLCAMMLASCAGVTVTVAGNAILDNWRVSEAFSSPALTRAFERELAPN
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHCCCCHHHHHCCCHHHHHHHHHHCCC
AALLAQARQSPEICRLVLHELSARVLTDRLPAIKGPNRVSRALDGGRVFVRYQWPDGVTC
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEEEECCCCCEE
RFPNGVGSAASSDAQGNGKTVTLTVRSPVEPGATLTVGMFVLSPLASLLHSDDVSWTVIG
ECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
LYVLVINFCSVLVLVPLLVKRIRKAERAASAWTDGDLAARIEDGGKDEFGRLTTSFDEMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHCCHHHHH
DALSSMIEMKLALAASQERNRLARDLHDTAKQRAFALGLQLTALKKHGAANVDSANLTRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
SLALVSHLQQDLADVIRRLSAPTIAEIGLRRALSDSIDALLAGARARWTLHLSHDDERAL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHH
EVVPDIARQLLLISIEACANVLKHAAATHVELAFSRLDDTYTLKIADDGQGFDARRVEAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHH
GMGLSNMRLRANSLPHGELHLASSPGKGAEITVSFQLTL
HCCHHHCEEECCCCCCCCEEEECCCCCCCEEEEEEEEEC
>Mature Secondary Structure 
AGRWRNSLFWRFLCAMMLASCAGVTVTVAGNAILDNWRVSEAFSSPALTRAFERELAPN
CCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHCCCCHHHHHCCCHHHHHHHHHHCCC
AALLAQARQSPEICRLVLHELSARVLTDRLPAIKGPNRVSRALDGGRVFVRYQWPDGVTC
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEEEECCCCCEE
RFPNGVGSAASSDAQGNGKTVTLTVRSPVEPGATLTVGMFVLSPLASLLHSDDVSWTVIG
ECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
LYVLVINFCSVLVLVPLLVKRIRKAERAASAWTDGDLAARIEDGGKDEFGRLTTSFDEMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHCCHHHHH
DALSSMIEMKLALAASQERNRLARDLHDTAKQRAFALGLQLTALKKHGAANVDSANLTRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
SLALVSHLQQDLADVIRRLSAPTIAEIGLRRALSDSIDALLAGARARWTLHLSHDDERAL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHH
EVVPDIARQLLLISIEACANVLKHAAATHVELAFSRLDDTYTLKIADDGQGFDARRVEAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHH
GMGLSNMRLRANSLPHGELHLASSPGKGAEITVSFQLTL
HCCHHHCEEECCCCCCCCEEEECCCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]