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The map label for this gene is ghrB [H]

Identifier: 91783830

GI number: 91783830

Start: 2737825

End: 2738790

Strand: Reverse

Name: ghrB [H]

Synonym: Bxe_A1982

Alternate gene names: 91783830

Gene position: 2738790-2737825 (Counterclockwise)

Preceding gene: 91783831

Following gene: 91783829

Centisome position: 55.94

GC content: 66.87

Gene sequence:

>966_bases
ATGAAAAAGATCGTCGCCTGGAAGTCGTTGCCCGAAGACGTGCTCGCGTATCTGCAGCAGCACGCGCAGGTCGTGCAGGT
CGACGCCGCGCAGCACGACGCGTTCGTGGCCGCGCTGAAAGACGCGGACGGCGGCATCGGTTCGAGCGTGAAGATCACGC
CGGCAATGCTCGAAGGCGCGACCCGGCTCAAGGCGCTGTCGACCATCTCGGTGGGCTTCGACCAGTTCGACGTGGCCGAC
CTCACGCGCCGCGGCATCGTGCTCGCGCACACGCCGGACGTGTTGACCGAATCCACCGCGGACACGGTGTTCTCGCTGAT
TCTCGCGTCGGCGCGGCGGGTGGTGGAACTCGCCGACTGGGTAAAGGCGGGGCACTGGCAGCACGGCATCGGGCCGGCGC
AGTTCGGCGTCGATGTGCAGGGCAAGACGCTCGGCATCGTCGGGCTCGGGCGGATTGGCGGCGCGGTGGCGCGGCGCGCG
GCGCTCGGCTTCAACATGAAGGTGCTGTACACCAACCGTAGCGCGAATCCGCAGGCGGAAGCGGCTTACGGTGCGCGGCG
CGTCGAGTTGGCGGAACTGCTCGCCACTGCGGATTTTGTCTGCCTGCAAGTGCCTTTGACGCCGGAGACGAAGTACCTGA
TCGGCGCGGCCGAACTCAGGTCGATGAAGAAGAGCGCGATCCTGATCAACGCCTCGCGCGGCGCGACCGTCGACGAAGCG
GCGCTGATCGAAGCCCTGCAGAACGGCACGATCCACGGCGCCGGTCTCGACGTGTTCGAGACCGAACCGCTGCCGGCCGA
CTCGCCGCTCCTGAAGCTCGCGAATGTGGTCGCGCTGCCGCACATCGGTTCGGCGACTCACGAAACCCGTTATGCGATGG
CGTGCAACGCGGCGGAAAACCTCGTCGCCGCGCTTAACGGCACGCTGGCCGTCAACATCGTCAACCGCGAAGTGCTCGCC
GGATGA

Upstream 100 bases:

>100_bases
CTCGTCGCCGCCGTGATTCTGACGCTGGTCGTCAAACCGCAGCCGCAAGCGCTGAACAATGCGCCGCCGCTCGCTACCCC
GTTGCAAGGAAAATAAGCTG

Downstream 100 bases:

>100_bases
ACCGGACGACAAGCCGAACCACAAGCCGAACCACAAGCCGCACCACAAGCCGAACCACGAGGAACCGATGAGCACGACGC
CGCAGCCCATGCCGCGCCGC

Product: putative 2-ketogluconate 6-phosphate reductase, TkrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKKIVAWKSLPEDVLAYLQQHAQVVQVDAAQHDAFVAALKDADGGIGSSVKITPAMLEGATRLKALSTISVGFDQFDVAD
LTRRGIVLAHTPDVLTESTADTVFSLILASARRVVELADWVKAGHWQHGIGPAQFGVDVQGKTLGIVGLGRIGGAVARRA
ALGFNMKVLYTNRSANPQAEAAYGARRVELAELLATADFVCLQVPLTPETKYLIGAAELRSMKKSAILINASRGATVDEA
ALIEALQNGTIHGAGLDVFETEPLPADSPLLKLANVVALPHIGSATHETRYAMACNAAENLVAALNGTLAVNIVNREVLA
G

Sequences:

>Translated_321_residues
MKKIVAWKSLPEDVLAYLQQHAQVVQVDAAQHDAFVAALKDADGGIGSSVKITPAMLEGATRLKALSTISVGFDQFDVAD
LTRRGIVLAHTPDVLTESTADTVFSLILASARRVVELADWVKAGHWQHGIGPAQFGVDVQGKTLGIVGLGRIGGAVARRA
ALGFNMKVLYTNRSANPQAEAAYGARRVELAELLATADFVCLQVPLTPETKYLIGAAELRSMKKSAILINASRGATVDEA
ALIEALQNGTIHGAGLDVFETEPLPADSPLLKLANVVALPHIGSATHETRYAMACNAAENLVAALNGTLAVNIVNREVLA
G
>Mature_321_residues
MKKIVAWKSLPEDVLAYLQQHAQVVQVDAAQHDAFVAALKDADGGIGSSVKITPAMLEGATRLKALSTISVGFDQFDVAD
LTRRGIVLAHTPDVLTESTADTVFSLILASARRVVELADWVKAGHWQHGIGPAQFGVDVQGKTLGIVGLGRIGGAVARRA
ALGFNMKVLYTNRSANPQAEAAYGARRVELAELLATADFVCLQVPLTPETKYLIGAAELRSMKKSAILINASRGATVDEA
ALIEALQNGTIHGAGLDVFETEPLPADSPLLKLANVVALPHIGSATHETRYAMACNAAENLVAALNGTLAVNIVNREVLA
G

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=245, Percent_Identity=44.4897959183673, Blast_Score=198, Evalue=5e-51,
Organism=Homo sapiens, GI23308577, Length=322, Percent_Identity=32.6086956521739, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI61743967, Length=281, Percent_Identity=33.8078291814947, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI4557497, Length=281, Percent_Identity=33.8078291814947, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI145580578, Length=280, Percent_Identity=31.7857142857143, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI4557499, Length=280, Percent_Identity=31.7857142857143, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI145580575, Length=280, Percent_Identity=31.7857142857143, Blast_Score=121, Evalue=8e-28,
Organism=Escherichia coli, GI87082289, Length=320, Percent_Identity=54.6875, Blast_Score=338, Evalue=3e-94,
Organism=Escherichia coli, GI1789279, Length=276, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787645, Length=204, Percent_Identity=30.3921568627451, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87081824, Length=285, Percent_Identity=28.0701754385965, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788660, Length=234, Percent_Identity=32.9059829059829, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17532191, Length=322, Percent_Identity=32.2981366459627, Blast_Score=140, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI25147481, Length=285, Percent_Identity=29.8245614035088, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324055, Length=240, Percent_Identity=38.75, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320925, Length=298, Percent_Identity=33.5570469798658, Blast_Score=132, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6322116, Length=298, Percent_Identity=33.2214765100671, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6325144, Length=178, Percent_Identity=30.8988764044944, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324964, Length=241, Percent_Identity=29.4605809128631, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321253, Length=176, Percent_Identity=30.6818181818182, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324980, Length=174, Percent_Identity=31.6091954022989, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI28571528, Length=288, Percent_Identity=42.0138888888889, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI45551003, Length=313, Percent_Identity=36.4217252396166, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI28574284, Length=313, Percent_Identity=36.4217252396166, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI45552429, Length=313, Percent_Identity=36.4217252396166, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24585514, Length=313, Percent_Identity=36.4217252396166, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI28574282, Length=313, Percent_Identity=36.4217252396166, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI28574286, Length=314, Percent_Identity=36.3057324840764, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24585516, Length=254, Percent_Identity=34.251968503937, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI19921140, Length=331, Percent_Identity=30.8157099697885, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24646446, Length=283, Percent_Identity=33.5689045936396, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24646448, Length=283, Percent_Identity=33.5689045936396, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24646452, Length=283, Percent_Identity=33.5689045936396, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24646450, Length=283, Percent_Identity=33.5689045936396, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI62472511, Length=283, Percent_Identity=33.5689045936396, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 33768; Mature: 33768

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVAWKSLPEDVLAYLQQHAQVVQVDAAQHDAFVAALKDADGGIGSSVKITPAMLEGA
CCCEECHHCCCHHHHHHHHHCCCEEEEECHHCCEEEEEEECCCCCCCCCEEECHHHHHHH
TRLKALSTISVGFDQFDVADLTRRGIVLAHTPDVLTESTADTVFSLILASARRVVELADW
HHHHHHHHEECCCCCCCHHHHHHCCEEEEECCCHHHCHHHHHHHHHHHHHHHHHHHHHHH
VKAGHWQHGIGPAQFGVDVQGKTLGIVGLGRIGGAVARRAALGFNMKVLYTNRSANPQAE
HHHCCCCCCCCHHHCCEEECCCEEEEEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCHH
AAYGARRVELAELLATADFVCLQVPLTPETKYLIGAAELRSMKKSAILINASRGATVDEA
HHHCHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEHHHHHHHCCCEEEEECCCCCCCHHH
ALIEALQNGTIHGAGLDVFETEPLPADSPLLKLANVVALPHIGSATHETRYAMACNAAEN
HHHHHHHCCCEEECCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHEEECCHHHH
LVAALNGTLAVNIVNREVLAG
HHHHHCCEEEEEEECCHHHCC
>Mature Secondary Structure
MKKIVAWKSLPEDVLAYLQQHAQVVQVDAAQHDAFVAALKDADGGIGSSVKITPAMLEGA
CCCEECHHCCCHHHHHHHHHCCCEEEEECHHCCEEEEEEECCCCCCCCCEEECHHHHHHH
TRLKALSTISVGFDQFDVADLTRRGIVLAHTPDVLTESTADTVFSLILASARRVVELADW
HHHHHHHHEECCCCCCCHHHHHHCCEEEEECCCHHHCHHHHHHHHHHHHHHHHHHHHHHH
VKAGHWQHGIGPAQFGVDVQGKTLGIVGLGRIGGAVARRAALGFNMKVLYTNRSANPQAE
HHHCCCCCCCCHHHCCEEECCCEEEEEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCHH
AAYGARRVELAELLATADFVCLQVPLTPETKYLIGAAELRSMKKSAILINASRGATVDEA
HHHCHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEHHHHHHHCCCEEEEECCCCCCCHHH
ALIEALQNGTIHGAGLDVFETEPLPADSPLLKLANVVALPHIGSATHETRYAMACNAAEN
HHHHHHHCCCEEECCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHEEECCHHHH
LVAALNGTLAVNIVNREVLAG
HHHHHCCEEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA