Click here to switch to the map view.

The map label for this gene is kgtP [H]

Identifier: 91783804

GI number: 91783804

Start: 2707606

End: 2708910

Strand: Reverse

Name: kgtP [H]

Synonym: Bxe_A2008

Alternate gene names: 91783804

Gene position: 2708910-2707606 (Counterclockwise)

Preceding gene: 91783805

Following gene: 91783800

Centisome position: 55.33

GC content: 64.29

Gene sequence:

>1305_bases
ATGACCGACCTAACCGACCAATCCGTGGCTTCGGCCCACGACACCCGGCGCCGCATCTTTGCGATCGTTGGCGCTTCATC
GGGCAATCTCGTCGAGTGGTTCGACTTTTACGTATATTCGTTCTGCGCGCTGTACTTCGCGCCGGCGTTCTTCCCGAGCG
GCAACCCGACCACGCAGCTGCTCAACACCGCGGGTGTGTTTGCCGCCGGCTTCCTGATGCGCCCGATCGGCGGCTGGTTC
TTCGGCCGCCTTGCCGACAAGCACGGGCGGCGCACCGCGATGATGGTGTCGGTGTTCATGATGTGCGGCGGCTCGCTGGT
GATCGCGGTGCTTCCTACTTACGCGCAGATCGGCGCGCTGGCGCCGGCGCTGCTGCTGCTCGCGCGGCTGTTCCAGGGGC
TGTCGGTGGGCGGCGAATACGGCACCAGTGCGACCTATATGAGTGAAGTCGCGCTCAAGGGGCGGCGTGGTTTCTTCGCG
TCGTTCCAGTACGTCACGCTGATTGGCGGGCAGTTGTGCGCGCTGCTGGTGCTGGTGGTGCTGCAACAGACGCTCTCCAC
CGAAGAGCTGAAGGCGTGGGGCTGGCGCGTGCCGTTCGTGATCGGCGCATTGGCGGCGTTGATCGCGCTGTATCTGCGTA
AATCGCTCGACGAAACCACCACGGCGGAGACGCGCCATCGCAAGGAAGCCGGCACGCTGCGCGGCCTCTGGCTGCACAAG
GGCGCGTTCATGACGGTGCTCGGCTTCACGGCCGGAGGCTCGCTGATTTTCTACACGTTCACCACCTACATGCAGAAGTA
CCTGGTCAACACGGCGGGCATGCACGCGAAGACGGCGAGCAACGTGATGACCGCGGCGCTGTTCGTCTACATGCTGATGC
AGCCGGCATTCGGCGCGTTGTCGGACCGCATCGGCCGGCGCCGCTCGATGCTGTTCTTCGGGTTCTTCGCAACCATCGGC
ACGGTGCCGCTGCTGCACGCGCTGAAAGACGTGACGAGCCCGTATGCAGCCTTTGGCCTGGTGGTGGCGGCGCTGGCGAT
CGTCAGTTTTTATACGTCGATCAGCGGCCTGATCAAGGCGGAGATGTTCCCGCCGGAAGTGCGCGCGCTCGGCGTGGGGT
TGTCGTATGCGGTGGCCAATGCGATTTTCGGCGGCTCGGCGGAATATGTCGCGCTGTGGCTGAAGCAGGCGGGCAGCGAG
TCCACGTTCTACTGGTATGTGACGGCCATGTGCGCGATCGCCGGTGTCGTAGCGTTCCTCATGCGTGACCCGTCGAAAGA
AGGGTATCTGCGGCACGCGCCGTGA

Upstream 100 bases:

>100_bases
CGCACGCGCGAGCCATGCCACCGGGGCACCTCGACGCGCGCGCTGTGAAGGCGGCGCCACGACCGCTAACTCCGTTTAAA
GACAAGGAATGAGAACAGGC

Downstream 100 bases:

>100_bases
GCGCTTGACGCACGACGACGAATGCGGTTCAGAAGCAGCGCCCCGATGTAACCGGCGCGCTACTTCTTCGCGGCCCGGGC
GATACGAGCGGGCCGCACGC

Product: major facilitator superfamily dicarboxlate/proton symporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTDLTDQSVASAHDTRRRIFAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPSGNPTTQLLNTAGVFAAGFLMRPIGGWF
FGRLADKHGRRTAMMVSVFMMCGGSLVIAVLPTYAQIGALAPALLLLARLFQGLSVGGEYGTSATYMSEVALKGRRGFFA
SFQYVTLIGGQLCALLVLVVLQQTLSTEELKAWGWRVPFVIGALAALIALYLRKSLDETTTAETRHRKEAGTLRGLWLHK
GAFMTVLGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTAALFVYMLMQPAFGALSDRIGRRRSMLFFGFFATIG
TVPLLHALKDVTSPYAAFGLVVAALAIVSFYTSISGLIKAEMFPPEVRALGVGLSYAVANAIFGGSAEYVALWLKQAGSE
STFYWYVTAMCAIAGVVAFLMRDPSKEGYLRHAP

Sequences:

>Translated_434_residues
MTDLTDQSVASAHDTRRRIFAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPSGNPTTQLLNTAGVFAAGFLMRPIGGWF
FGRLADKHGRRTAMMVSVFMMCGGSLVIAVLPTYAQIGALAPALLLLARLFQGLSVGGEYGTSATYMSEVALKGRRGFFA
SFQYVTLIGGQLCALLVLVVLQQTLSTEELKAWGWRVPFVIGALAALIALYLRKSLDETTTAETRHRKEAGTLRGLWLHK
GAFMTVLGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTAALFVYMLMQPAFGALSDRIGRRRSMLFFGFFATIG
TVPLLHALKDVTSPYAAFGLVVAALAIVSFYTSISGLIKAEMFPPEVRALGVGLSYAVANAIFGGSAEYVALWLKQAGSE
STFYWYVTAMCAIAGVVAFLMRDPSKEGYLRHAP
>Mature_433_residues
TDLTDQSVASAHDTRRRIFAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPSGNPTTQLLNTAGVFAAGFLMRPIGGWFF
GRLADKHGRRTAMMVSVFMMCGGSLVIAVLPTYAQIGALAPALLLLARLFQGLSVGGEYGTSATYMSEVALKGRRGFFAS
FQYVTLIGGQLCALLVLVVLQQTLSTEELKAWGWRVPFVIGALAALIALYLRKSLDETTTAETRHRKEAGTLRGLWLHKG
AFMTVLGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTAALFVYMLMQPAFGALSDRIGRRRSMLFFGFFATIGT
VPLLHALKDVTSPYAAFGLVVAALAIVSFYTSISGLIKAEMFPPEVRALGVGLSYAVANAIFGGSAEYVALWLKQAGSES
TFYWYVTAMCAIAGVVAFLMRDPSKEGYLRHAP

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=425, Percent_Identity=72.2352941176471, Blast_Score=586, Evalue=1e-168,
Organism=Escherichia coli, GI1790550, Length=437, Percent_Identity=29.7482837528604, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1788292, Length=430, Percent_Identity=29.5348837209302, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1789941, Length=439, Percent_Identity=31.2072892938497, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI87082231, Length=309, Percent_Identity=27.1844660194175, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082404, Length=315, Percent_Identity=26.3492063492064, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47096; Mature: 46964

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCCCCCCC
>Mature Secondary Structure 
TDLTDQSVASAHDTRRRIFAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPSGNPTTQL
CCCCCHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH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HCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]