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The map label for this gene is gatA [H]

Identifier: 91783714

GI number: 91783714

Start: 2607706

End: 2608830

Strand: Direct

Name: gatA [H]

Synonym: Bxe_A2100

Alternate gene names: 91783714

Gene position: 2607706-2608830 (Clockwise)

Preceding gene: 91783713

Following gene: 91783715

Centisome position: 53.26

GC content: 67.73

Gene sequence:

>1125_bases
ATGAACGAGTTTATCCAGACCCTGTCGTTAGGCAGCAGCGGTCCGAGCATCGCGATCAAGGACACGATCGATATCGCCGG
CTACCCCACCACCGCCGCGAGCCGCGCGCTGGCGGATACGCCACCCGCGCGGCGGCATGCGCAGGTGGTTGAACGGTTGC
TGGCGGCGGGCTGGCATATCGTCGGCAAAACGAACATGCATGAACTGGCGTTCGGCATGACCGGCATCAACGACTACACC
GGCACGCCGCAGAATCCGCAGGACGCCACGCGCATTCCGGGCGGATCGTCGAGCGGATCGGCGGCCGCGGTCGGGCTGAA
ACTCGCCGACGCCGCGCTCGGCACCGACACCGGAGGTTCCATTCGCGGCCCCGCCGCATGCTGCGGCGTGATCGGATTGA
AGCCCACCTTCGGGCGCGTGTCGCGGCTCGGCGTGGCGCCGCGAGAATCCACGCTCGACTGTGTCGGGCCATTTGCGCGC
GACATGCGCATGCTCGCCGCCGCGATGCTCGCCATAACCGCGGATTTCGATGCGAACGCCGCGCACGCGTGGCAAGGCCC
GTGCAAGGTCGGTATCGTGCAGGCCGACGCCACGGCCGACATTCTCGCCGCCGTGGCACGCGCCGCCGACACGGCCAACT
GTACCGCGCATACGTTGCAACTCACGGGTCTCGCAGCCGCTTTCGACGCGGGCCTCACCGTCATCAACGCCGAGACTTCG
CGCGCGTTCGGGCATCTGGTGGCAGGCGGAAAGATCGGCGCGGATCTCGACGCGCGCCTGCGCGCGGCAGCCAACACCAC
CGCCGCGCAAGTCGATGCGGCGGAACAGGTGCGCCGCCAGTTCACGGCGGCCATCGACCACGCGCTCGACAACGCCGACG
TATTGATTCTGCCCACACTGCCCGCTCTGCCCATCACCCTCGAAGAAGCGCGCAGCGGCAAGTCCGTGATCGCGATGTCC
TCGCTGATCCGGCCGTTCAATCTGAGCGGGCATCCCGCGTTGAGTTTGCCGCTGCCGGTTGAAGGCTCGCCGCTCAAGGC
GGGGTTGCAGATCGTGGGACGCAAGGGGCATGACGAGCAGGTGTGCGCAATCGCGGCACGTTTCGAAGCCGCCCTTGCCG
CCTGA

Upstream 100 bases:

>100_bases
TCGCGCCTGCCGGCAATGGCCGCGACTGGCTCATCACGCACTTTCGCACGGAACGCCTGTTCGACGCGCCGTGGCAAGTC
AATGCAAGGAAACCGCAGAC

Downstream 100 bases:

>100_bases
CGCAGGCATCAAGTCATGCCGCCCGCGCGGCTGGCTGTTTTATCCGGAGCGCAAGCAATGCAGAAGAGAGTCGTACTCGT
CACCGGCGCCGCGCGTGGTC

Product: putative Asp-tRNA Asn/Glu-tRNAGln amidotransferase A subunit

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MNEFIQTLSLGSSGPSIAIKDTIDIAGYPTTAASRALADTPPARRHAQVVERLLAAGWHIVGKTNMHELAFGMTGINDYT
GTPQNPQDATRIPGGSSSGSAAAVGLKLADAALGTDTGGSIRGPAACCGVIGLKPTFGRVSRLGVAPRESTLDCVGPFAR
DMRMLAAAMLAITADFDANAAHAWQGPCKVGIVQADATADILAAVARAADTANCTAHTLQLTGLAAAFDAGLTVINAETS
RAFGHLVAGGKIGADLDARLRAAANTTAAQVDAAEQVRRQFTAAIDHALDNADVLILPTLPALPITLEEARSGKSVIAMS
SLIRPFNLSGHPALSLPLPVEGSPLKAGLQIVGRKGHDEQVCAIAARFEAALAA

Sequences:

>Translated_374_residues
MNEFIQTLSLGSSGPSIAIKDTIDIAGYPTTAASRALADTPPARRHAQVVERLLAAGWHIVGKTNMHELAFGMTGINDYT
GTPQNPQDATRIPGGSSSGSAAAVGLKLADAALGTDTGGSIRGPAACCGVIGLKPTFGRVSRLGVAPRESTLDCVGPFAR
DMRMLAAAMLAITADFDANAAHAWQGPCKVGIVQADATADILAAVARAADTANCTAHTLQLTGLAAAFDAGLTVINAETS
RAFGHLVAGGKIGADLDARLRAAANTTAAQVDAAEQVRRQFTAAIDHALDNADVLILPTLPALPITLEEARSGKSVIAMS
SLIRPFNLSGHPALSLPLPVEGSPLKAGLQIVGRKGHDEQVCAIAARFEAALAA
>Mature_374_residues
MNEFIQTLSLGSSGPSIAIKDTIDIAGYPTTAASRALADTPPARRHAQVVERLLAAGWHIVGKTNMHELAFGMTGINDYT
GTPQNPQDATRIPGGSSSGSAAAVGLKLADAALGTDTGGSIRGPAACCGVIGLKPTFGRVSRLGVAPRESTLDCVGPFAR
DMRMLAAAMLAITADFDANAAHAWQGPCKVGIVQADATADILAAVARAADTANCTAHTLQLTGLAAAFDAGLTVINAETS
RAFGHLVAGGKIGADLDARLRAAANTTAAQVDAAEQVRRQFTAAIDHALDNADVLILPTLPALPITLEEARSGKSVIAMS
SLIRPFNLSGHPALSLPLPVEGSPLKAGLQIVGRKGHDEQVCAIAARFEAALAA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=173, Percent_Identity=34.6820809248555, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI195972892, Length=165, Percent_Identity=32.7272727272727, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=405, Percent_Identity=29.6296296296296, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17537465, Length=132, Percent_Identity=37.1212121212121, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=216, Percent_Identity=30.5555555555556, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=171, Percent_Identity=29.8245614035088, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=415, Percent_Identity=29.6385542168675, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319685, Length=381, Percent_Identity=30.7086614173228, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648113, Length=427, Percent_Identity=29.03981264637, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI45550774, Length=408, Percent_Identity=25.2450980392157, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648435, Length=408, Percent_Identity=25.2450980392157, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648437, Length=408, Percent_Identity=25.2450980392157, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648441, Length=408, Percent_Identity=25.2450980392157, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648439, Length=408, Percent_Identity=25.2450980392157, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356731, Length=127, Percent_Identity=37.7952755905512, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24652985, Length=354, Percent_Identity=25.9887005649718, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19922090, Length=354, Percent_Identity=25.9887005649718, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24652981, Length=354, Percent_Identity=25.9887005649718, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24652983, Length=354, Percent_Identity=25.9887005649718, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 38170; Mature: 38170

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEFIQTLSLGSSGPSIAIKDTIDIAGYPTTAASRALADTPPARRHAQVVERLLAAGWHI
CCHHHHHHHCCCCCCCEEEEECEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCEEE
VGKTNMHELAFGMTGINDYTGTPQNPQDATRIPGGSSSGSAAAVGLKLADAALGTDTGGS
ECCCCHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEEEHHHHHCCCCCCCC
IRGPAACCGVIGLKPTFGRVSRLGVAPRESTLDCVGPFARDMRMLAAAMLAITADFDANA
CCCHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AHAWQGPCKVGIVQADATADILAAVARAADTANCTAHTLQLTGLAAAFDAGLTVINAETS
CCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHCCEEEEECCHH
RAFGHLVAGGKIGADLDARLRAAANTTAAQVDAAEQVRRQFTAAIDHALDNADVLILPTL
HHHHHEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
PALPITLEEARSGKSVIAMSSLIRPFNLSGHPALSLPLPVEGSPLKAGLQIVGRKGHDEQ
CCCCEEHHHHCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHHEECCCCCCHH
VCAIAARFEAALAA
HHHHHHHHHHHHCC
>Mature Secondary Structure
MNEFIQTLSLGSSGPSIAIKDTIDIAGYPTTAASRALADTPPARRHAQVVERLLAAGWHI
CCHHHHHHHCCCCCCCEEEEECEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCEEE
VGKTNMHELAFGMTGINDYTGTPQNPQDATRIPGGSSSGSAAAVGLKLADAALGTDTGGS
ECCCCHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEEEHHHHHCCCCCCCC
IRGPAACCGVIGLKPTFGRVSRLGVAPRESTLDCVGPFARDMRMLAAAMLAITADFDANA
CCCHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AHAWQGPCKVGIVQADATADILAAVARAADTANCTAHTLQLTGLAAAFDAGLTVINAETS
CCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHCCEEEEECCHH
RAFGHLVAGGKIGADLDARLRAAANTTAAQVDAAEQVRRQFTAAIDHALDNADVLILPTL
HHHHHEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
PALPITLEEARSGKSVIAMSSLIRPFNLSGHPALSLPLPVEGSPLKAGLQIVGRKGHDEQ
CCCCEEHHHHCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHHEECCCCCCHH
VCAIAARFEAALAA
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA