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The map label for this gene is rimO [H]

Identifier: 91783479

GI number: 91783479

Start: 2324396

End: 2325781

Strand: Direct

Name: rimO [H]

Synonym: Bxe_A2337

Alternate gene names: 91783479

Gene position: 2324396-2325781 (Clockwise)

Preceding gene: 91783478

Following gene: 91783480

Centisome position: 47.48

GC content: 62.19

Gene sequence:

>1386_bases
ATGTCTGCCACCCCACCGATTGCCCCGATTGCCACGCCGAAAGTCGGCTTTGTCTCGCTCGGGTGCCCGAAAGCCCTGGT
CGATTCCGAGCAGATCATCACCCAATTGCGCGCCGAAGGTTACGAGATTTCCGGCACGTACGACGGCGCGGACCTCGTGG
TCGTCAACACCTGCGGTTTCATCGACGAAGCAGTGCAGGAGAGCCTCGACGCGATCGGCGAAGCGCTCAACGAAAATGGC
AAGGTGATCGTGACCGGCTGCCTCGGCGCCAAAAAGAGCGCGAGCGGTTCGGGGCTGATCGAAGAAGTGCATCCCAAGGT
GCTGGCCGTGACCGGCCCGCACGCGCTCGGCGAAGTCATGCAGCACGTGCATATGCATCTGCCGAAGCCGCACGATCCGT
TCGTCGACCTCGTGCCGGCCGCGGGGGTGAAACTCACGCCGCGCCATTACGCGTATCTGAAGATTTCCGAAGGGTGTAAT
CATCGTTGCACGTTCTGCATCATCCCGTCCATGCGCGGTGACCTGGTGTCGCGTCCGGTCGCCGACGTCATGCTCGAAGC
GGAAAATCTGTTCAAGTCCGGCGTGAAGGAACTGCTGGTGATTTCGCAGGACACCAGCGCGTACGGCGTGGACGTCAAGT
ACCGCACCGGATTCTGGAACGGCAAGCCGATCAAGACGCGCATGACCGATCTGGTCGGCGCGCTCGGCGAACTCGCCGCG
CAATACGGCGCATGGGTGCGTCTGCATTACGTGTATCCGTATCCGAGTGTGGACGAAGTGATTCCGATGATGGCCGAAGG
TCCGTTCAAGGGCCACGTGCTGCCGTATCTGGACGTGCCGTTCCAGCACGCGCATCCGGAAGTGCTGAAGCGCATGAAGC
GCCCGGCCAACGCCGAGAAGGTGATGGAGCGCGTGAAGAAGTGGCGCGAGATGTGCCCGGATCTGACCATTCGCAGCACG
TTTATCGCCGGCTTCCCAGGCGAGACTGAAGAGCAGTTCCAGACGCTGCTCGATTTCATTCGCGAGGCGGAACTGGACCG
GGTCGGCTGCTTCGCCTATTCGCCGGTCGAAGGCGCGACGGCCAACGAACTCGACGGCGCGTTGCCCGACGAAGTGCGTG
AAGAGCGTCGCGCGCGTTTCATGGAAGTTGCCGAAGAAGTATCGGCGAAGCGTATCGCGAAGAAGGTCGGCAAGACGCTG
AAGGTGCTGGTCGACGAGATCAACGCCGACGGCGGCATCGGCCGCACGGCGGCCGACGCGCCTGAGATCGACGGCGTGGT
GTATATCGCGCCGGCTGTAAAGGCGTCCAAACGTTACAAGGTCGGCGATTTCGTGTCGGTGAAGATCACCGGCGCCGACG
GTCACGACCTGTGGGGCGAGGTATAG

Upstream 100 bases:

>100_bases
GGACCTCGCAGCCGGTTGAAGGCGAAAATTCAAAGACTTACGAGCGGCAGCCCTGGCGAACCGCTACAATTCAGGGTTTA
ACGTCTTCAAGCGGTTCCCC

Downstream 100 bases:

>100_bases
GCAATGGCGGCGAGCATGAGCGCAACGCCGGAGGTCCAGCGTCCGGACATCCTCGCGCTCGGCGAGGCGATGATCGAATT
CAACCAGTCGGTCAAAGACG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSATPPIAPIATPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALNENG
KVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGPHALGEVMQHVHMHLPKPHDPFVDLVPAAGVKLTPRHYAYLKISEGCN
HRCTFCIIPSMRGDLVSRPVADVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVGALGELAA
QYGAWVRLHYVYPYPSVDEVIPMMAEGPFKGHVLPYLDVPFQHAHPEVLKRMKRPANAEKVMERVKKWREMCPDLTIRST
FIAGFPGETEEQFQTLLDFIREAELDRVGCFAYSPVEGATANELDGALPDEVREERRARFMEVAEEVSAKRIAKKVGKTL
KVLVDEINADGGIGRTAADAPEIDGVVYIAPAVKASKRYKVGDFVSVKITGADGHDLWGEV

Sequences:

>Translated_461_residues
MSATPPIAPIATPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALNENG
KVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGPHALGEVMQHVHMHLPKPHDPFVDLVPAAGVKLTPRHYAYLKISEGCN
HRCTFCIIPSMRGDLVSRPVADVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVGALGELAA
QYGAWVRLHYVYPYPSVDEVIPMMAEGPFKGHVLPYLDVPFQHAHPEVLKRMKRPANAEKVMERVKKWREMCPDLTIRST
FIAGFPGETEEQFQTLLDFIREAELDRVGCFAYSPVEGATANELDGALPDEVREERRARFMEVAEEVSAKRIAKKVGKTL
KVLVDEINADGGIGRTAADAPEIDGVVYIAPAVKASKRYKVGDFVSVKITGADGHDLWGEV
>Mature_460_residues
SATPPIAPIATPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALNENGK
VIVTGCLGAKKSASGSGLIEEVHPKVLAVTGPHALGEVMQHVHMHLPKPHDPFVDLVPAAGVKLTPRHYAYLKISEGCNH
RCTFCIIPSMRGDLVSRPVADVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVGALGELAAQ
YGAWVRLHYVYPYPSVDEVIPMMAEGPFKGHVLPYLDVPFQHAHPEVLKRMKRPANAEKVMERVKKWREMCPDLTIRSTF
IAGFPGETEEQFQTLLDFIREAELDRVGCFAYSPVEGATANELDGALPDEVREERRARFMEVAEEVSAKRIAKKVGKTLK
VLVDEINADGGIGRTAADAPEIDGVVYIAPAVKASKRYKVGDFVSVKITGADGHDLWGEV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=417, Percent_Identity=26.8585131894484, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI28872782, Length=493, Percent_Identity=25.9634888438134, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI28872784, Length=493, Percent_Identity=25.9634888438134, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1787057, Length=448, Percent_Identity=64.2857142857143, Blast_Score=610, Evalue=1e-176,
Organism=Escherichia coli, GI1786882, Length=465, Percent_Identity=26.0215053763441, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17553146, Length=491, Percent_Identity=23.6252545824847, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71996771, Length=302, Percent_Identity=27.1523178807947, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356207, Length=488, Percent_Identity=27.2540983606557, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19922432, Length=407, Percent_Identity=28.009828009828, Blast_Score=118, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50236; Mature: 50105

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATPPIAPIATPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGF
CCCCCCCCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCCEEEEECCCH
IDEAVQESLDAIGEALNENGKVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGPHALGEVM
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHCCCEEEEECCHHHHHHH
QHVHMHLPKPHDPFVDLVPAAGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPV
HHHHHCCCCCCCCHHHHHCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHCCH
ADVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVGALGELAA
HHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
QYGAWVRLHYVYPYPSVDEVIPMMAEGPFKGHVLPYLDVPFQHAHPEVLKRMKRPANAEK
HHCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCHHH
VMERVKKWREMCPDLTIRSTFIAGFPGETEEQFQTLLDFIREAELDRVGCFAYSPVEGAT
HHHHHHHHHHHCCCCEEEHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
ANELDGALPDEVREERRARFMEVAEEVSAKRIAKKVGKTLKVLVDEINADGGIGRTAADA
CHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PEIDGVVYIAPAVKASKRYKVGDFVSVKITGADGHDLWGEV
CCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SATPPIAPIATPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGF
CCCCCCCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCCEEEEECCCH
IDEAVQESLDAIGEALNENGKVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGPHALGEVM
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHCCCEEEEECCHHHHHHH
QHVHMHLPKPHDPFVDLVPAAGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPV
HHHHHCCCCCCCCHHHHHCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHCCH
ADVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVGALGELAA
HHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
QYGAWVRLHYVYPYPSVDEVIPMMAEGPFKGHVLPYLDVPFQHAHPEVLKRMKRPANAEK
HHCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCHHH
VMERVKKWREMCPDLTIRSTFIAGFPGETEEQFQTLLDFIREAELDRVGCFAYSPVEGAT
HHHHHHHHHHHCCCCEEEHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
ANELDGALPDEVREERRARFMEVAEEVSAKRIAKKVGKTLKVLVDEINADGGIGRTAADA
CHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PEIDGVVYIAPAVKASKRYKVGDFVSVKITGADGHDLWGEV
CCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA