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The map label for this gene is virB8 [H]

Identifier: 91783153

GI number: 91783153

Start: 1999582

End: 2000286

Strand: Direct

Name: virB8 [H]

Synonym: Bxe_A2652

Alternate gene names: 91783153

Gene position: 1999582-2000286 (Clockwise)

Preceding gene: 91783152

Following gene: 91783154

Centisome position: 40.84

GC content: 64.26

Gene sequence:

>705_bases
ATGAGCGCGCCGCACGACTACGGGCGTGTGCTCGACATCGAGACGTCGCTCACTCACCTGCAGGAACGCTCCGAGCGGCG
GGCCTGGCACGTCGCCTATGCAGCGGTCGGCGTGGCCGTGCTCAGTACCGCAGCACTCGCGGTGATGGTGCCGTTCTATC
GCGTCGTGCCGCTGCCGATCGAGGTCGATCGCCTGACGGGTGAGTCTCAGGTCATCGATGTGCTCGATGCCCACCATGTG
CATACGAAGGAAATCCAGGACAGGCACTGGGTCGAGACCTATGTGCGTGCGCGCGAGCGTTACGACTGGGGCCTGCTGCA
GATGGACTACGACAACGTGCTCACCATGAGCGATGACGTGGTCGCGCGCGATTACCGCGGCACCTACAGCGGTCCCGACG
CGCTGGACCGGCAACTGGGGTCCGGCGCCGAGCGGCGTGTTCGCATTCTCTCAATCACGCTGCCGCCAGACGAACCGGGT
CACGCGGTCGTGCATATCGAGCGCACATCGTTCAGGAACGGCCAGGCCGACGCCGAGCCGACGCAGCGTTTCGTGATCAC
GTTGGCCTATACCTATCAGCCATCCGTGTTCACGCGCGAGAGCGTGGCCATTGCCAATCCGTTCGGATTCAAGGTCACGG
CCTACAGCCGCGATCCCGAATACACGCCGCAGGCGTCGGCGTCGAATGCCGGAGTTAAGCCATGA

Upstream 100 bases:

>100_bases
CACATCGTGTGCCAGTCAATCGCGTGCCGCCCGTACCTGATGTGCCGGACGTGCAGGTTGCGCCGAACCTGCTCGCTTCC
CCGGACGCGGGAGACCGGTC

Downstream 100 bases:

>100_bases
AAACGTTTCTCTCTGCGCTTCTTTCCGGTGTGGCACTGGTGAGCCTGCTGTTCTCGGGCGTGACCCATGCAGTCGACCTC
CCCGGATGGGACGACGACTC

Product: putative type IV secretion system protein VirB8/TraJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSAPHDYGRVLDIETSLTHLQERSERRAWHVAYAAVGVAVLSTAALAVMVPFYRVVPLPIEVDRLTGESQVIDVLDAHHV
HTKEIQDRHWVETYVRARERYDWGLLQMDYDNVLTMSDDVVARDYRGTYSGPDALDRQLGSGAERRVRILSITLPPDEPG
HAVVHIERTSFRNGQADAEPTQRFVITLAYTYQPSVFTRESVAIANPFGFKVTAYSRDPEYTPQASASNAGVKP

Sequences:

>Translated_234_residues
MSAPHDYGRVLDIETSLTHLQERSERRAWHVAYAAVGVAVLSTAALAVMVPFYRVVPLPIEVDRLTGESQVIDVLDAHHV
HTKEIQDRHWVETYVRARERYDWGLLQMDYDNVLTMSDDVVARDYRGTYSGPDALDRQLGSGAERRVRILSITLPPDEPG
HAVVHIERTSFRNGQADAEPTQRFVITLAYTYQPSVFTRESVAIANPFGFKVTAYSRDPEYTPQASASNAGVKP
>Mature_233_residues
SAPHDYGRVLDIETSLTHLQERSERRAWHVAYAAVGVAVLSTAALAVMVPFYRVVPLPIEVDRLTGESQVIDVLDAHHVH
TKEIQDRHWVETYVRARERYDWGLLQMDYDNVLTMSDDVVARDYRGTYSGPDALDRQLGSGAERRVRILSITLPPDEPGH
AVVHIERTSFRNGQADAEPTQRFVITLAYTYQPSVFTRESVAIANPFGFKVTAYSRDPEYTPQASASNAGVKP

Specific function: The virB operon is essential for intracellular survival and is not involved in the invasion process. Constitutes a major determinant of virulence in mice [H]

COG id: COG3736

COG function: function code U; Type IV secretory pathway, component VirB8

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the virB8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007430 [H]

Pfam domain/function: PF04335 VirB8 [H]

EC number: NA

Molecular weight: Translated: 26296; Mature: 26165

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPHDYGRVLDIETSLTHLQERSERRAWHVAYAAVGVAVLSTAALAVMVPFYRVVPLPI
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EVDRLTGESQVIDVLDAHHVHTKEIQDRHWVETYVRARERYDWGLLQMDYDNVLTMSDDV
EEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCE
VARDYRGTYSGPDALDRQLGSGAERRVRILSITLPPDEPGHAVVHIERTSFRNGQADAEP
EECCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCC
TQRFVITLAYTYQPSVFTRESVAIANPFGFKVTAYSRDPEYTPQASASNAGVKP
CEEEEEEEEEEECCCCEECCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAPHDYGRVLDIETSLTHLQERSERRAWHVAYAAVGVAVLSTAALAVMVPFYRVVPLPI
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EVDRLTGESQVIDVLDAHHVHTKEIQDRHWVETYVRARERYDWGLLQMDYDNVLTMSDDV
EEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCE
VARDYRGTYSGPDALDRQLGSGAERRVRILSITLPPDEPGHAVVHIERTSFRNGQADAEP
EECCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCC
TQRFVITLAYTYQPSVFTRESVAIANPFGFKVTAYSRDPEYTPQASASNAGVKP
CEEEEEEEEEEECCCCEECCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10940027 [H]