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The map label for this gene is maiA [H]

Identifier: 91783087

GI number: 91783087

Start: 1933494

End: 1934138

Strand: Direct

Name: maiA [H]

Synonym: Bxe_A2723

Alternate gene names: 91783087

Gene position: 1933494-1934138 (Clockwise)

Preceding gene: 91783086

Following gene: 91783089

Centisome position: 39.49

GC content: 61.86

Gene sequence:

>645_bases
ATGAAGCTCTACAGCTACTTTCGCAGCTCGGCCTCGTACCGGGTACGCATCGCACTGAACCTCAAGGGCCTCGACTACGA
GTATGTGCCGATCCACCTGCTGCGCAATGGAGGCGAGCAGCTAACCCCCGCGTATCGTGAACTGAATCCGGACGGACTCG
TACCGACACTCGTTGAGGGGGGCGCCCCCCTCAACCAGTCGCTCGCTATCCTCGAATATCTTGAGGAAACGCATCCGGAG
CCGCCGCTATTGCCGCGCGAGCCGCTTGCACGCGCTCAAGTGCGCGCCTTCGCGTTGCAGATTGCGTGCGAGATCCATCC
GATTAACAATTTGCGCGTGCTCCGGTATCTGACCAGCGAGCTCGGCGCAAGTGAGGGCGCGAAGAACGCGTGGTACCGCC
ACTGGATCCAGGCAGGCTTCGCTTCGCTCGAAGCGCAGCTCGTGCGAGGTCAGCACACGGGTACGTTCTGTTACGGCGAT
ACGCCGACATTCGCTGACCTGTGCCTCGTGCCGCAGGTGTTCAACGGCCAGCGCTTCGAAATCGATATACAGCACTATCC
GACGCTCCAGCGCATCTACGATGCCGCGATGCGACTCGACGCGTTCGCAAACGCAGCGCCGGCCCAGCAGCCCGACGCCG
AATAG

Upstream 100 bases:

>100_bases
GCGGTGATCATCCGTGGATGGTGCGAGAAGGCGGGTGCGGCACGCATCGGCTTCGGCGAGTGTCGTGGCGCGATTTTGCC
CGCGCGTGCGGTGTGACGCA

Downstream 100 bases:

>100_bases
ACTGTCGTTCGCATGATGATGCGCACAGACCTTTTTGCATGCGCCGACACGAGCGCTTCGTCAATCTCGAGTTCGGAACC
TTACGGGTACAACACCCTGC

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKLYSYFRSSASYRVRIALNLKGLDYEYVPIHLLRNGGEQLTPAYRELNPDGLVPTLVEGGAPLNQSLAILEYLEETHPE
PPLLPREPLARAQVRAFALQIACEIHPINNLRVLRYLTSELGASEGAKNAWYRHWIQAGFASLEAQLVRGQHTGTFCYGD
TPTFADLCLVPQVFNGQRFEIDIQHYPTLQRIYDAAMRLDAFANAAPAQQPDAE

Sequences:

>Translated_214_residues
MKLYSYFRSSASYRVRIALNLKGLDYEYVPIHLLRNGGEQLTPAYRELNPDGLVPTLVEGGAPLNQSLAILEYLEETHPE
PPLLPREPLARAQVRAFALQIACEIHPINNLRVLRYLTSELGASEGAKNAWYRHWIQAGFASLEAQLVRGQHTGTFCYGD
TPTFADLCLVPQVFNGQRFEIDIQHYPTLQRIYDAAMRLDAFANAAPAQQPDAE
>Mature_214_residues
MKLYSYFRSSASYRVRIALNLKGLDYEYVPIHLLRNGGEQLTPAYRELNPDGLVPTLVEGGAPLNQSLAILEYLEETHPE
PPLLPREPLARAQVRAFALQIACEIHPINNLRVLRYLTSELGASEGAKNAWYRHWIQAGFASLEAQLVRGQHTGTFCYGD
TPTFADLCLVPQVFNGQRFEIDIQHYPTLQRIYDAAMRLDAFANAAPAQQPDAE

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=212, Percent_Identity=48.5849056603774, Blast_Score=193, Evalue=9e-50,
Organism=Homo sapiens, GI194440732, Length=212, Percent_Identity=38.2075471698113, Blast_Score=134, Evalue=6e-32,
Organism=Homo sapiens, GI22202622, Length=161, Percent_Identity=44.7204968944099, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17551302, Length=210, Percent_Identity=42.8571428571429, Blast_Score=173, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17510461, Length=211, Percent_Identity=40.2843601895735, Blast_Score=157, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17556142, Length=212, Percent_Identity=40.5660377358491, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24645375, Length=211, Percent_Identity=45.4976303317536, Blast_Score=178, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21355859, Length=211, Percent_Identity=45.4976303317536, Blast_Score=178, Evalue=2e-45,
Organism=Drosophila melanogaster, GI45553325, Length=211, Percent_Identity=45.4976303317536, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21355857, Length=212, Percent_Identity=42.4528301886792, Blast_Score=170, Evalue=7e-43,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24070; Mature: 24070

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYSYFRSSASYRVRIALNLKGLDYEYVPIHLLRNGGEQLTPAYRELNPDGLVPTLVEG
CCHHHHHHCCCCEEEEEEEEECCCCEEEEEEEEEECCCCCCCCHHHHCCCCCCCHHHHCC
GAPLNQSLAILEYLEETHPEPPLLPREPLARAQVRAFALQIACEIHPINNLRVLRYLTSE
CCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHH
LGASEGAKNAWYRHWIQAGFASLEAQLVRGQHTGTFCYGDTPTFADLCLVPQVFNGQRFE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEE
IDIQHYPTLQRIYDAAMRLDAFANAAPAQQPDAE
EECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKLYSYFRSSASYRVRIALNLKGLDYEYVPIHLLRNGGEQLTPAYRELNPDGLVPTLVEG
CCHHHHHHCCCCEEEEEEEEECCCCEEEEEEEEEECCCCCCCCHHHHCCCCCCCHHHHCC
GAPLNQSLAILEYLEETHPEPPLLPREPLARAQVRAFALQIACEIHPINNLRVLRYLTSE
CCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHH
LGASEGAKNAWYRHWIQAGFASLEAQLVRGQHTGTFCYGDTPTFADLCLVPQVFNGQRFE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEE
IDIQHYPTLQRIYDAAMRLDAFANAAPAQQPDAE
EECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]