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The map label for this gene is leuD [H]

Identifier: 91783079

GI number: 91783079

Start: 1924517

End: 1925155

Strand: Direct

Name: leuD [H]

Synonym: Bxe_A2731

Alternate gene names: 91783079

Gene position: 1924517-1925155 (Clockwise)

Preceding gene: 91783078

Following gene: 91783080

Centisome position: 39.31

GC content: 58.22

Gene sequence:

>639_bases
GTGCAACCCTTTACGAAACTGGAGGCGCTGGTGGTGCCGCTCGACCGCGTGAATGTCGACACCGACGCGATCATCCCCAA
GCAGTTTATGAAATCGGTGCAACGTAACGGCTTCGGCATCAATCTGTTCGATGAATGGCGCTACTTCGATCACGGAGAGC
CGGGACAGGATCCGTCGACCCGTCGGTTGAATCCCGATTTCGTACTCAATCAACCACGTTTCGCGGGTGCGGAGATTCTC
CTCACGCGCGACAACTTCGGGTGTGGATCGAGTCGCGAGCATGCAGCGTGGGCGTTGTGGGACTTCGGTATCCGAGCGCT
GATCGCACCAGGCTTTGCCGACATCTTCTACGGCAACTGCTTCAAGAACGGCATGCTGCCGATCAAGCTCGATGAACGTA
TCGTTCAGCAGTTGTTCGATCTCGTTGGGCGTACTCCCGGCCTGAGGCTAGCAATCGATCTGGAGGCTCAGCGCATTCAA
CCACCAGCCGGTGATGCGATTCCGTTCGACGTCGAGCCCGAGCGCAAACGCCGCTTGCTCAATGGGCTGGACGACGTCGC
TCTGACATTGCAGCAAACCGATCAGATCCGTGCGTATGAACGCAATCGCCGCGCCCATGAGCCCTGGCTTTTCGCCTGA

Upstream 100 bases:

>100_bases
GTCAGGGGCCTGGTGGCCGCAGCCACCTCGTCAGTCCTGCGATGGCAGCCGCGGCAGCGATTGCAGGTCACTTCGTTGAC
GTTTCCCACGGAGGCTTCTA

Downstream 100 bases:

>100_bases
TGCCAGGCACGCGCGCACGAACCGCAATTTCAGGAGCTTTTGTTATGAGTGGTCAACCTACCTTTCCGACTTCACCCGCT
GCGCTGAACGGCGTACGAGT

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MQPFTKLEALVVPLDRVNVDTDAIIPKQFMKSVQRNGFGINLFDEWRYFDHGEPGQDPSTRRLNPDFVLNQPRFAGAEIL
LTRDNFGCGSSREHAAWALWDFGIRALIAPGFADIFYGNCFKNGMLPIKLDERIVQQLFDLVGRTPGLRLAIDLEAQRIQ
PPAGDAIPFDVEPERKRRLLNGLDDVALTLQQTDQIRAYERNRRAHEPWLFA

Sequences:

>Translated_212_residues
MQPFTKLEALVVPLDRVNVDTDAIIPKQFMKSVQRNGFGINLFDEWRYFDHGEPGQDPSTRRLNPDFVLNQPRFAGAEIL
LTRDNFGCGSSREHAAWALWDFGIRALIAPGFADIFYGNCFKNGMLPIKLDERIVQQLFDLVGRTPGLRLAIDLEAQRIQ
PPAGDAIPFDVEPERKRRLLNGLDDVALTLQQTDQIRAYERNRRAHEPWLFA
>Mature_212_residues
MQPFTKLEALVVPLDRVNVDTDAIIPKQFMKSVQRNGFGINLFDEWRYFDHGEPGQDPSTRRLNPDFVLNQPRFAGAEIL
LTRDNFGCGSSREHAAWALWDFGIRALIAPGFADIFYGNCFKNGMLPIKLDERIVQQLFDLVGRTPGLRLAIDLEAQRIQ
PPAGDAIPFDVEPERKRRLLNGLDDVALTLQQTDQIRAYERNRRAHEPWLFA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=205, Percent_Identity=54.1463414634146, Blast_Score=218, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321429, Length=214, Percent_Identity=50, Blast_Score=201, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 24248; Mature: 24248

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPFTKLEALVVPLDRVNVDTDAIIPKQFMKSVQRNGFGINLFDEWRYFDHGEPGQDPST
CCCHHHHHHEEECHHHCCCCCCCCCHHHHHHHHHHCCCEEEEHHCCCCCCCCCCCCCCCC
RRLNPDFVLNQPRFAGAEILLTRDNFGCGSSREHAAWALWDFGIRALIAPGFADIFYGNC
CCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHH
FKNGMLPIKLDERIVQQLFDLVGRTPGLRLAIDLEAQRIQPPAGDAIPFDVEPERKRRLL
HHCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCCCCCCCCCCHHHHHHHH
NGLDDVALTLQQTDQIRAYERNRRAHEPWLFA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQPFTKLEALVVPLDRVNVDTDAIIPKQFMKSVQRNGFGINLFDEWRYFDHGEPGQDPST
CCCHHHHHHEEECHHHCCCCCCCCCHHHHHHHHHHCCCEEEEHHCCCCCCCCCCCCCCCC
RRLNPDFVLNQPRFAGAEILLTRDNFGCGSSREHAAWALWDFGIRALIAPGFADIFYGNC
CCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHH
FKNGMLPIKLDERIVQQLFDLVGRTPGLRLAIDLEAQRIQPPAGDAIPFDVEPERKRRLL
HHCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCCCCCCCCCCHHHHHHHH
NGLDDVALTLQQTDQIRAYERNRRAHEPWLFA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA