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The map label for this gene is leuC [H]

Identifier: 91783078

GI number: 91783078

Start: 1923084

End: 1924517

Strand: Direct

Name: leuC [H]

Synonym: Bxe_A2732

Alternate gene names: 91783078

Gene position: 1923084-1924517 (Clockwise)

Preceding gene: 91783077

Following gene: 91783079

Centisome position: 39.28

GC content: 63.25

Gene sequence:

>1434_bases
ATGGAACCTGCGACTTCACCCCGTACGCTGCTCGACAAGCTGTGGCAAAGCCACGTCGTCGCGGAAACGCCCAACGGCCC
AACGCTGCTTTATGTTGACCGCCATCTCGTTTACGAGGTGACAAGCCCACAAGCGTTCGAGGCGATTCGCCTGTCGGGCC
GCAAACCCTGGCGGCCTGAAACAGTACTCGCGGTCGCTGACCATAACGTACCGACGATCGCCGCGGAGCGCACGAGCATG
GACGCGATCGCTGACCCACTCTCGCGCATTCAGGTCGCGCAGCTCGACAAAAATTGCAAGGAGTTTGGTATCAAGTCGTA
CGGTATCCGGAACCCGCAGCAAGGAATCATCCACGTAGTCGGCCCGGAACTCGGGGCGACGTTGCCGGGCATGACGGTCG
TGGCGGGAGACTCGCACACGAGTACCCACGGTGCGTTCGCCGCACTGGCGTTCGGGGTGGGCACGTCGGAAGTCGAGCAC
GTGCTGGCAACCCAATGCCTGTCGGTTGGGAAAATGAAGTCGATGCTCGTGAACGTTGAGGGCGTACTGCCGGTGGGCGT
CACGGCCAAGGACGTGATTCTCGCGATCATCCGCAGGACCGGTACCGCCGGCGGCACCGGTTACGCAATGGAATTCGCGG
GCAGCACGATCCGCACGCTGTCGATGGAGGGTCGCATGACCCTGTGCAACATGGCGATCGAGGCGGGCGCCCGGGTGGGG
CTGATCGGCGTCGACGATGTCACGATCGACTATGTGAAGGGGCGTCCCTTCGCGCCCGCAGAAGCACACTGGGATGCGGC
GGTCGCCTATTGGCGCACGCTGGTCAGCGATGCGGACGCCCGGTTCGACAAGATCGTCAACATCGACGCAACGCAGCTAC
GGCCGATGGTTACCTGGGGCACGTCGCCCGAAATGGTGGTCACGGTCGACGACGCTGTACCGAATCCGTTGGACGATCCC
GATCCCGTTCGCCGCGCCACCATGGCCGGCGCGCTTACGTATATGGGACTTGAGCCAGGTACGTCGCTCAAGAGCATTTC
ACTCGACAAGATTTTCATCGGCTCCTGCACGAATGCACGCATCGAGGATCTGCGTGCAGCCGCCGCGATCGTCAAGGGCA
GGCACGTCGCGCCGACCGTCCAGCTTGCGCTGGTGGTGCCCGGCTCGGGTCTCGTCAAGGCACAGGCCGAGGCCGAGGGG
CTGGACGCCATATTCAAGGAGGCCGGCTTTGAATGGCGCGAACCGGGTTGTTCGATGTGCCTCGGCATGAACGACGACCG
CCTGCGTCCGGGGGAGCGGTGTGCGTCTACATCTAACCGAAATTTTGAAGGCCGTCAGGGGCCTGGTGGCCGCAGCCACC
TCGTCAGTCCTGCGATGGCAGCCGCGGCAGCGATTGCAGGTCACTTCGTTGACGTTTCCCACGGAGGCTTCTAG

Upstream 100 bases:

>100_bases
AGCATCGCGCGTGGACAATAGATGTCCCTATATCGCAGCGTCGCGGGCTGCGGTGATAGCAAAACCAGCATGCTCTCATC
CTGAATATGGAGGCTCTCCC

Downstream 100 bases:

>100_bases
TGCAACCCTTTACGAAACTGGAGGCGCTGGTGGTGCCGCTCGACCGCGTGAATGTCGACACCGACGCGATCATCCCCAAG
CAGTTTATGAAATCGGTGCA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MEPATSPRTLLDKLWQSHVVAETPNGPTLLYVDRHLVYEVTSPQAFEAIRLSGRKPWRPETVLAVADHNVPTIAAERTSM
DAIADPLSRIQVAQLDKNCKEFGIKSYGIRNPQQGIIHVVGPELGATLPGMTVVAGDSHTSTHGAFAALAFGVGTSEVEH
VLATQCLSVGKMKSMLVNVEGVLPVGVTAKDVILAIIRRTGTAGGTGYAMEFAGSTIRTLSMEGRMTLCNMAIEAGARVG
LIGVDDVTIDYVKGRPFAPAEAHWDAAVAYWRTLVSDADARFDKIVNIDATQLRPMVTWGTSPEMVVTVDDAVPNPLDDP
DPVRRATMAGALTYMGLEPGTSLKSISLDKIFIGSCTNARIEDLRAAAAIVKGRHVAPTVQLALVVPGSGLVKAQAEAEG
LDAIFKEAGFEWREPGCSMCLGMNDDRLRPGERCASTSNRNFEGRQGPGGRSHLVSPAMAAAAAIAGHFVDVSHGGF

Sequences:

>Translated_477_residues
MEPATSPRTLLDKLWQSHVVAETPNGPTLLYVDRHLVYEVTSPQAFEAIRLSGRKPWRPETVLAVADHNVPTIAAERTSM
DAIADPLSRIQVAQLDKNCKEFGIKSYGIRNPQQGIIHVVGPELGATLPGMTVVAGDSHTSTHGAFAALAFGVGTSEVEH
VLATQCLSVGKMKSMLVNVEGVLPVGVTAKDVILAIIRRTGTAGGTGYAMEFAGSTIRTLSMEGRMTLCNMAIEAGARVG
LIGVDDVTIDYVKGRPFAPAEAHWDAAVAYWRTLVSDADARFDKIVNIDATQLRPMVTWGTSPEMVVTVDDAVPNPLDDP
DPVRRATMAGALTYMGLEPGTSLKSISLDKIFIGSCTNARIEDLRAAAAIVKGRHVAPTVQLALVVPGSGLVKAQAEAEG
LDAIFKEAGFEWREPGCSMCLGMNDDRLRPGERCASTSNRNFEGRQGPGGRSHLVSPAMAAAAAIAGHFVDVSHGGF
>Mature_477_residues
MEPATSPRTLLDKLWQSHVVAETPNGPTLLYVDRHLVYEVTSPQAFEAIRLSGRKPWRPETVLAVADHNVPTIAAERTSM
DAIADPLSRIQVAQLDKNCKEFGIKSYGIRNPQQGIIHVVGPELGATLPGMTVVAGDSHTSTHGAFAALAFGVGTSEVEH
VLATQCLSVGKMKSMLVNVEGVLPVGVTAKDVILAIIRRTGTAGGTGYAMEFAGSTIRTLSMEGRMTLCNMAIEAGARVG
LIGVDDVTIDYVKGRPFAPAEAHWDAAVAYWRTLVSDADARFDKIVNIDATQLRPMVTWGTSPEMVVTVDDAVPNPLDDP
DPVRRATMAGALTYMGLEPGTSLKSISLDKIFIGSCTNARIEDLRAAAAIVKGRHVAPTVQLALVVPGSGLVKAQAEAEG
LDAIFKEAGFEWREPGCSMCLGMNDDRLRPGERCASTSNRNFEGRQGPGGRSHLVSPAMAAAAAIAGHFVDVSHGGF

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI8659555, Length=454, Percent_Identity=26.8722466960352, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI4501867, Length=369, Percent_Identity=27.10027100271, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI41352693, Length=368, Percent_Identity=27.7173913043478, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1786259, Length=467, Percent_Identity=62.3126338329765, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1787531, Length=370, Percent_Identity=27.2972972972973, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI2367097, Length=369, Percent_Identity=25.2032520325203, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=371, Percent_Identity=29.3800539083558, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32564738, Length=371, Percent_Identity=29.3800539083558, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=452, Percent_Identity=27.6548672566372, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25149342, Length=302, Percent_Identity=28.8079470198675, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=60.1694915254237, Blast_Score=572, Evalue=1e-164,
Organism=Saccharomyces cerevisiae, GI6323335, Length=374, Percent_Identity=31.5508021390374, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322261, Length=384, Percent_Identity=28.3854166666667, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320440, Length=463, Percent_Identity=26.5658747300216, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI281365315, Length=384, Percent_Identity=28.125, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17864292, Length=384, Percent_Identity=28.125, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161076999, Length=384, Percent_Identity=28.125, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28571643, Length=480, Percent_Identity=26.25, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24645686, Length=442, Percent_Identity=28.0542986425339, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17137564, Length=485, Percent_Identity=27.4226804123711, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50642; Mature: 50642

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPATSPRTLLDKLWQSHVVAETPNGPTLLYVDRHLVYEVTSPQAFEAIRLSGRKPWRPE
CCCCCCHHHHHHHHHHCCEEEECCCCCEEEEEECEEEEEECCCHHHHHHHHCCCCCCCCC
TVLAVADHNVPTIAAERTSMDAIADPLSRIQVAQLDKNCKEFGIKSYGIRNPQQGIIHVV
EEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
GPELGATLPGMTVVAGDSHTSTHGAFAALAFGVGTSEVEHVLATQCLSVGKMKSMLVNVE
CCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEC
GVLPVGVTAKDVILAIIRRTGTAGGTGYAMEFAGSTIRTLSMEGRMTLCNMAIEAGARVG
CCEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCCCHHHHHHHHCCCEEE
LIGVDDVTIDYVKGRPFAPAEAHWDAAVAYWRTLVSDADARFDKIVNIDATQLRPMVTWG
EEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHEECCCHHHCCEEEEEC
TSPEMVVTVDDAVPNPLDDPDPVRRATMAGALTYMGLEPGTSLKSISLDKIFIGSCTNAR
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCCCEEEECEEEEECCCCCH
IEDLRAAAAIVKGRHVAPTVQLALVVPGSGLVKAQAEAEGLDAIFKEAGFEWREPGCSMC
HHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEECCHHHHHHHHHHHCCCCCCCCCCCEE
LGMNDDRLRPGERCASTSNRNFEGRQGPGGRSHLVSPAMAAAAAIAGHFVDVSHGGF
ECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHEEEECCCCC
>Mature Secondary Structure
MEPATSPRTLLDKLWQSHVVAETPNGPTLLYVDRHLVYEVTSPQAFEAIRLSGRKPWRPE
CCCCCCHHHHHHHHHHCCEEEECCCCCEEEEEECEEEEEECCCHHHHHHHHCCCCCCCCC
TVLAVADHNVPTIAAERTSMDAIADPLSRIQVAQLDKNCKEFGIKSYGIRNPQQGIIHVV
EEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
GPELGATLPGMTVVAGDSHTSTHGAFAALAFGVGTSEVEHVLATQCLSVGKMKSMLVNVE
CCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEC
GVLPVGVTAKDVILAIIRRTGTAGGTGYAMEFAGSTIRTLSMEGRMTLCNMAIEAGARVG
CCEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCCCHHHHHHHHCCCEEE
LIGVDDVTIDYVKGRPFAPAEAHWDAAVAYWRTLVSDADARFDKIVNIDATQLRPMVTWG
EEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHEECCCHHHCCEEEEEC
TSPEMVVTVDDAVPNPLDDPDPVRRATMAGALTYMGLEPGTSLKSISLDKIFIGSCTNAR
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCCCEEEECEEEEECCCCCH
IEDLRAAAAIVKGRHVAPTVQLALVVPGSGLVKAQAEAEGLDAIFKEAGFEWREPGCSMC
HHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEECCHHHHHHHHHHHCCCCCCCCCCCEE
LGMNDDRLRPGERCASTSNRNFEGRQGPGGRSHLVSPAMAAAAAIAGHFVDVSHGGF
ECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]