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The map label for this gene is sucB [H]

Identifier: 91782998

GI number: 91782998

Start: 1837830

End: 1839113

Strand: Direct

Name: sucB [H]

Synonym: Bxe_A2811

Alternate gene names: 91782998

Gene position: 1837830-1839113 (Clockwise)

Preceding gene: 91782997

Following gene: 91782999

Centisome position: 37.54

GC content: 63.55

Gene sequence:

>1284_bases
ATGGCTATTGTTGAAGTCAAGGTCCCCCAGCTTTCCGAGTCGGTTTCGGAAGCCACCATGCTGCAGTGGAAGAAGAAGCC
CGGCGAGGCTGTTGCTCAAGACGAAATTCTCATCGAAATCGAGACCGACAAGGTCGTGCTCGAAGTGCCGGCACCCTCGG
CCGGCGTGCTCGCGCAAGTCATCGCGAACGACGGCGACACCGTCACGGCTGATCAGGTGATCGCCAAGATCGACACCGAA
GGCACGGCAAGCGCCGCTGCTGTCGAAGCCGAAGTCAAGCCCGCTCCGCAAGCCGCACCGGCACCCGCCGCTGCACCGGC
TCCGGCGGCTCAAGCCGCATCCGCAACGGGTGCGAACACCGCTGCTTCGCCGGCCGCCGGCAAGCTGATGGCCGAGAAGG
GTCTGGGCGCGGGCGACGTCGCCGGCACGGGCCGCGACGGCCGCATCACCAAGGGCGACGTGCTGACCGCGGGCGCACCG
GCTGCGAAGGCTGCCGCCCCGGCTCCGGCCGCCGCACCGAAGGCAGCGAAGCCGTCGCTGCCGGACGTCAAGGCGCCGGC
CTCGGCTGAGCAATGGCTGAAGGACCGTCCGGAACAACGCGTGCCGATGTCGCGTCTGCGTGCGCGTATCGCCGAACGTC
TGCTCGAGTCGCAGCAAACCAACGCCATTCTCACCACGTTCAACGAAGTGAACATGGCGCCGGTCATGGACCTGCGCAAC
AAGTACAAAGACAAGTTCGAAAAGGAACATGGCGTGAAGCTCGGCTTCATGTCGTTCTTCGTGAAGGCGGCGGTCCACGC
GCTGAAGAAGTTCCCGCTGGTGAACGCGTCGATCGACGGTAACGATATCGTCTATCACGGCTACTTCGACATCGGTATCG
CTGTCGGTTCGCCGCGCGGTCTGGTGGTGCCGATCCTGCGCAACGCGGATCAGCTGAGCCTCGCTGAAATCGAGAAGAAG
ATTGCTGAATTCGGCCAGAAGGCGAAAGACGGCAAGCTGTCGATCGAAGAAATGACCGGCGGCACGTTCTCGATCTCGAA
CGGTGGCGTGTTCGGTTCGATGCTGTCGACCCCGATCATCAACCCGCCGCAATCCGCGATTCTGGGCGTGCACGCCACGA
AGGAACGCGCCGTGGTCGAAAACGGCCAGATCGTGATCCGCCCGATGAACTATCTGGCGCTGTCGTACGACCACCGCATC
ATCGACGGTCGCGAAGCGGTGCTGTCGCTGGTCGCCATGAAGGATGCGCTGGAAGATCCGGCTCGCCTGCTGCTCGACCT
GTAA

Upstream 100 bases:

>100_bases
CCGCATCCGGAAAGATCAGGTGCGGCATTCGAGGTTCGCAGGCGGTCGTAGGGTCCCCAGATCGCGCGCCGTCACCCGAT
ACGTATTCAGGATATTCATA

Downstream 100 bases:

>100_bases
GCGCCGGTTCGGATGGTGCCGGCTTGTCATCGTGCAAGCCGGCACCATATAAGCAACGGGTAAGCATCACGTCTTTCGCA
TTCCGCAGTACAGGAACGGC

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLAQVIANDGDTVTADQVIAKIDTE
GTASAAAVEAEVKPAPQAAPAPAAAPAPAAQAASATGANTAASPAAGKLMAEKGLGAGDVAGTGRDGRITKGDVLTAGAP
AAKAAAPAPAAAPKAAKPSLPDVKAPASAEQWLKDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMDLRN
KYKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKK
IAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERAVVENGQIVIRPMNYLALSYDHRI
IDGREAVLSLVAMKDALEDPARLLLDL

Sequences:

>Translated_427_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLAQVIANDGDTVTADQVIAKIDTE
GTASAAAVEAEVKPAPQAAPAPAAAPAPAAQAASATGANTAASPAAGKLMAEKGLGAGDVAGTGRDGRITKGDVLTAGAP
AAKAAAPAPAAAPKAAKPSLPDVKAPASAEQWLKDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMDLRN
KYKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKK
IAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERAVVENGQIVIRPMNYLALSYDHRI
IDGREAVLSLVAMKDALEDPARLLLDL
>Mature_426_residues
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLAQVIANDGDTVTADQVIAKIDTEG
TASAAAVEAEVKPAPQAAPAPAAAPAPAAQAASATGANTAASPAAGKLMAEKGLGAGDVAGTGRDGRITKGDVLTAGAPA
AKAAAPAPAAAPKAAKPSLPDVKAPASAEQWLKDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMDLRNK
YKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKKI
AEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERAVVENGQIVIRPMNYLALSYDHRII
DGREAVLSLVAMKDALEDPARLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=55.982905982906, Blast_Score=281, Evalue=8e-76,
Organism=Homo sapiens, GI31711992, Length=450, Percent_Identity=31.1111111111111, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI110671329, Length=440, Percent_Identity=28.8636363636364, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI203098753, Length=460, Percent_Identity=30.2173913043478, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI203098816, Length=460, Percent_Identity=30.2173913043478, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.2670807453416, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786946, Length=427, Percent_Identity=52.9274004683841, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1786305, Length=430, Percent_Identity=32.5581395348837, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=430, Percent_Identity=43.2558139534884, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17560088, Length=454, Percent_Identity=31.057268722467, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=448, Percent_Identity=26.5625, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=32.4675324675325, Blast_Score=135, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6320352, Length=422, Percent_Identity=42.1800947867299, Blast_Score=330, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6324258, Length=462, Percent_Identity=30.5194805194805, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24645909, Length=241, Percent_Identity=55.6016597510373, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI18859875, Length=440, Percent_Identity=27.9545454545455, Blast_Score=147, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=34.2105263157895, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=34.2105263157895, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44675; Mature: 44544

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLAQV
CEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHH
IANDGDTVTADQVIAKIDTEGTASAAAVEAEVKPAPQAAPAPAAAPAPAAQAASATGANT
HHCCCCEEEHHHHHHCCCCCCCCCHHEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCC
AASPAAGKLMAEKGLGAGDVAGTGRDGRITKGDVLTAGAPAAKAAAPAPAAAPKAAKPSL
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHCCCCCCCCCCCCCCCCC
PDVKAPASAEQWLKDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMDLRN
CCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
KYKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRG
HHHHHHHHHHCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCC
LVVPILRNADQLSLAEIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPII
EEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCCCCC
NPPQSAILGVHATKERAVVENGQIVIRPMNYLALSYDHRIIDGREAVLSLVAMKDALEDP
CCCHHHEEEEECCCCCCEEECCCEEEEEHHHEEEECCCEEECCHHHHHHHHHHHHHHCCH
ARLLLDL
HHHHHCC
>Mature Secondary Structure 
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLAQV
EEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHH
IANDGDTVTADQVIAKIDTEGTASAAAVEAEVKPAPQAAPAPAAAPAPAAQAASATGANT
HHCCCCEEEHHHHHHCCCCCCCCCHHEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCC
AASPAAGKLMAEKGLGAGDVAGTGRDGRITKGDVLTAGAPAAKAAAPAPAAAPKAAKPSL
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHCCCCCCCCCCCCCCCCC
PDVKAPASAEQWLKDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMDLRN
CCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
KYKDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRG
HHHHHHHHHHCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCC
LVVPILRNADQLSLAEIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPII
EEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCCCCC
NPPQSAILGVHATKERAVVENGQIVIRPMNYLALSYDHRIIDGREAVLSLVAMKDALEDP
CCCHHHEEEEECCCCCCEEECCCEEEEEHHHEEEECCCEEECCHHHHHHHHHHHHHHCCH
ARLLLDL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]