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The map label for this gene is shiA [H]

Identifier: 91782821

GI number: 91782821

Start: 1590915

End: 1592228

Strand: Direct

Name: shiA [H]

Synonym: Bxe_A3004

Alternate gene names: 91782821

Gene position: 1590915-1592228 (Clockwise)

Preceding gene: 91782820

Following gene: 91782822

Centisome position: 32.5

GC content: 66.59

Gene sequence:

>1314_bases
ATGAATCGCGAGCCAGACGTCCACGCCGCAGGCCGGCAACCCACGCGCGCCGCAGCCGCGGCCTTTGTCGGCACCACGAT
CGAGTGGTACGACTTTTATATCTACGCCACGGCGTCCGCGCTGATCTTCGGCAAGCTGTTCTTTCCAGGCGGCGATCCGT
TCTTCGCCACGCTGGCTTCGTTCGGCACTTTCGCGGTCGGCTTCTTCGCGCGACCGTTCGGCGGGCTGGTGTTCGGCCAC
CTTGGCGACCGCATCGGCCGCAAGAAGGCGCTCGTGGCGACGCTCGCGATCATGGGCGTCGGCACCGTCGGCATCGGCTT
TCTGCCGACTTACGCGAGCGCCGGCGTGTGGGCGCCGGTGCTGCTCGTACTGCTGCGCGTCGCGCAGGGGATCGCGATCG
GCGGCGAGTGGGGCGGCGCGGTGCTGATGGCGAGCGAACATGCGCCGCAAGGGCGGCGAACCTTCTTCGCCTCGTTCGCG
CAACTCGGTAGTCCGGCGGGCCTGATCCTGTCGCTGCTCGCGTTCCGCGCGGTGGCCTCGATGGACAAGGACGCGTTTCT
CAGCTGGGGATGGCGTCTGCCGTTTCTCGCCAGCGCAGTGCTGCTGGTGGTCGGCCTGCTGATCCGCGCGGGTGTGGACG
AATCGCCGGAATTCAAGGCGCTCAAGGCGCAACGGCGAGTGGCCGCGCTACCCGTGGCCGAAGTCGTGCGCGATGCGTGG
CGCACGCTGTTGCTGTGCCTCGGCGCGAACGTGATCGGCGTGGCCGGCGCGTGGTTCATCAATACTTTCATGCTCAATTA
CACGACGCAGACGCTCGGCCTCGACCGGTCGTTGATTCTCGACTGCCTGTTCGTCGTAGCGTTCATTCAGTTGTTCACGC
AACTAGGTTCGGGATGGTTCGCGCAACGCATCGGCACGGGACGTTTCCTGAAGGGCGCGGCAGCGCTGGCAATGCTGTCG
CCGTATCCCATGTTCGCGCTCGTCTCGACCGGACGGCCGGTGGCGATCGTGATCGGCATTGCGCTGGCGGTGATGTGCAT
GTCGAGTTCGTATGCGGTGATGGCGGGGTTCATGTCGACCGCGTTCGCCGTGCGCGTCAGGTACTCGGCGATTTCGCTGT
CGTACCAGGTGTGCGCCGCGCTGGCCGGCGGCCTGACGCCGCTGATCGGAACGTTGCTCGCGCATCGGTATCCGGGCGCC
TGGTGGCCGCTTGCCGTGTTCTATAGTGTTCTGGCCGGGGTGTCGCTGGTGTGCATCAGTACGCTGGAGCGCCGCAAGCG
TGGCGCGGCGCATGGCGTGGCCGAAGCGGCCTAG

Upstream 100 bases:

>100_bases
CGTGCTGAAAGCGGGCGCACCTGAAGCATAAGCGCAGCAGTTCCCGGTGACGGCACGAAGAAGCCGCGCCATCCAGAAGA
ACGACCTTGGAGACACAGAC

Downstream 100 bases:

>100_bases
CACTCTGCGCGTGTGATTGTGCGCGTGCCGCCGACGCCCGGAAACGCGAGGAAATGTGATTTTCGTGGTTAACCCCACGT
CATAGTCCCCCGACATAATG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MNREPDVHAAGRQPTRAAAAAFVGTTIEWYDFYIYATASALIFGKLFFPGGDPFFATLASFGTFAVGFFARPFGGLVFGH
LGDRIGRKKALVATLAIMGVGTVGIGFLPTYASAGVWAPVLLVLLRVAQGIAIGGEWGGAVLMASEHAPQGRRTFFASFA
QLGSPAGLILSLLAFRAVASMDKDAFLSWGWRLPFLASAVLLVVGLLIRAGVDESPEFKALKAQRRVAALPVAEVVRDAW
RTLLLCLGANVIGVAGAWFINTFMLNYTTQTLGLDRSLILDCLFVVAFIQLFTQLGSGWFAQRIGTGRFLKGAAALAMLS
PYPMFALVSTGRPVAIVIGIALAVMCMSSSYAVMAGFMSTAFAVRVRYSAISLSYQVCAALAGGLTPLIGTLLAHRYPGA
WWPLAVFYSVLAGVSLVCISTLERRKRGAAHGVAEAA

Sequences:

>Translated_437_residues
MNREPDVHAAGRQPTRAAAAAFVGTTIEWYDFYIYATASALIFGKLFFPGGDPFFATLASFGTFAVGFFARPFGGLVFGH
LGDRIGRKKALVATLAIMGVGTVGIGFLPTYASAGVWAPVLLVLLRVAQGIAIGGEWGGAVLMASEHAPQGRRTFFASFA
QLGSPAGLILSLLAFRAVASMDKDAFLSWGWRLPFLASAVLLVVGLLIRAGVDESPEFKALKAQRRVAALPVAEVVRDAW
RTLLLCLGANVIGVAGAWFINTFMLNYTTQTLGLDRSLILDCLFVVAFIQLFTQLGSGWFAQRIGTGRFLKGAAALAMLS
PYPMFALVSTGRPVAIVIGIALAVMCMSSSYAVMAGFMSTAFAVRVRYSAISLSYQVCAALAGGLTPLIGTLLAHRYPGA
WWPLAVFYSVLAGVSLVCISTLERRKRGAAHGVAEAA
>Mature_437_residues
MNREPDVHAAGRQPTRAAAAAFVGTTIEWYDFYIYATASALIFGKLFFPGGDPFFATLASFGTFAVGFFARPFGGLVFGH
LGDRIGRKKALVATLAIMGVGTVGIGFLPTYASAGVWAPVLLVLLRVAQGIAIGGEWGGAVLMASEHAPQGRRTFFASFA
QLGSPAGLILSLLAFRAVASMDKDAFLSWGWRLPFLASAVLLVVGLLIRAGVDESPEFKALKAQRRVAALPVAEVVRDAW
RTLLLCLGANVIGVAGAWFINTFMLNYTTQTLGLDRSLILDCLFVVAFIQLFTQLGSGWFAQRIGTGRFLKGAAALAMLS
PYPMFALVSTGRPVAIVIGIALAVMCMSSSYAVMAGFMSTAFAVRVRYSAISLSYQVCAALAGGLTPLIGTLLAHRYPGA
WWPLAVFYSVLAGVSLVCISTLERRKRGAAHGVAEAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=411, Percent_Identity=37.4695863746959, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1790550, Length=433, Percent_Identity=32.1016166281755, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1789941, Length=424, Percent_Identity=35.1415094339623, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1788942, Length=423, Percent_Identity=31.2056737588652, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI87082231, Length=237, Percent_Identity=24.0506329113924, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46298; Mature: 46298

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREPDVHAAGRQPTRAAAAAFVGTTIEWYDFYIYATASALIFGKLFFPGGDPFFATLAS
CCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
FGTFAVGFFARPFGGLVFGHLGDRIGRKKALVATLAIMGVGTVGIGFLPTYASAGVWAPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LLVLLRVAQGIAIGGEWGGAVLMASEHAPQGRRTFFASFAQLGSPAGLILSLLAFRAVAS
HHHHHHHHCCCEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
MDKDAFLSWGWRLPFLASAVLLVVGLLIRAGVDESPEFKALKAQRRVAALPVAEVVRDAW
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
RTLLLCLGANVIGVAGAWFINTFMLNYTTQTLGLDRSLILDCLFVVAFIQLFTQLGSGWF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHH
AQRIGTGRFLKGAAALAMLSPYPMFALVSTGRPVAIVIGIALAVMCMSSSYAVMAGFMST
HHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
AFAVRVRYSAISLSYQVCAALAGGLTPLIGTLLAHRYPGAWWPLAVFYSVLAGVSLVCIS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
TLERRKRGAAHGVAEAA
HHHHHHCCCCCCHHCCC
>Mature Secondary Structure
MNREPDVHAAGRQPTRAAAAAFVGTTIEWYDFYIYATASALIFGKLFFPGGDPFFATLAS
CCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
FGTFAVGFFARPFGGLVFGHLGDRIGRKKALVATLAIMGVGTVGIGFLPTYASAGVWAPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LLVLLRVAQGIAIGGEWGGAVLMASEHAPQGRRTFFASFAQLGSPAGLILSLLAFRAVAS
HHHHHHHHCCCEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
MDKDAFLSWGWRLPFLASAVLLVVGLLIRAGVDESPEFKALKAQRRVAALPVAEVVRDAW
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
RTLLLCLGANVIGVAGAWFINTFMLNYTTQTLGLDRSLILDCLFVVAFIQLFTQLGSGWF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHH
AQRIGTGRFLKGAAALAMLSPYPMFALVSTGRPVAIVIGIALAVMCMSSSYAVMAGFMST
HHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
AFAVRVRYSAISLSYQVCAALAGGLTPLIGTLLAHRYPGAWWPLAVFYSVLAGVSLVCIS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
TLERRKRGAAHGVAEAA
HHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]