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The map label for this gene is nuoM [H]

Identifier: 91782624

GI number: 91782624

Start: 1394418

End: 1395908

Strand: Direct

Name: nuoM [H]

Synonym: Bxe_A3202

Alternate gene names: 91782624

Gene position: 1394418-1395908 (Clockwise)

Preceding gene: 91782623

Following gene: 91782625

Centisome position: 28.48

GC content: 60.3

Gene sequence:

>1491_bases
ATGCACGCTTATCCGATTCTCAGCATCGCAATCTGGTTACCGATTCTCGTCGGCCTGCTGGTCCTCGCTATCGGTTCGGA
CCGGAACCCGGCTCCCGCGCGCTGGATTGCGCTCATCGGCTCGGTCGTCAGCTTCCTCGTGACGATCCCGCTGATTACAG
GTTTTGATTCGAGCACCGCCGATCTGCAGTTCGTCGAAAAGGCGAACTGGATCGAGCGCTTCAACATTACGTACCACCTG
GGTGTCGACGGCATCTCGATGTGGTTCGTCGTGTTGACGGCCCTGATTACGGTGATCGTCGTGATCGCCGCGTGGGAAGT
GATCACGAAGAACGTGGCGCAGTACCTCGCCGCGTTCCTGATCCTCTCGGGCATCATGGTCGGCGTGTTCAGCGCGGCCG
ACGGCATGCTGTTCTACGTGTTCTTCGAAGCGACGCTGATTCCGATGTACATCATCATCGGCGTGTGGGGCGGGGCGAAC
CGCGTGTACGCGGCATTCAAGTTTTTCCTGTACACGCTGATGGGCTCGCTGCTGATGCTGGTCGCGCTGCTGTACCTGTA
CGTCCAGACCGGCACGTTCGACCTCGCCACGTGGCAGCATGCGCAGATCGCCATGACGCCTCAGGTGTTGCTGTTCATAG
CGTTCTTCATGGCGTTCGCCGTGAAGGTGCCGATGTGGCCGGTTCACACCTGGTTGCCCGACGCCCACGTGGAAGCGCCG
ACCGGCGGCTCGGTCGTGCTGGCCGCGATCATGCTGAAGCTGGGCGCATACGGTTTCCTGCGCTTCTCGTTGCCGATCAC
GCCGGACGCGAGTCACTTCCTCGCGCCGGTCGTCATCACGCTGTCGCTGATCGCGGTGATCTACATCGGCCTCGTCGCGA
TGGTGCAGGCGGACATGAAGAAGCTGGTCGCGTATTCGTCGATCGCGCACATGGGTTTCGTCACGCTCGGCTTCTTCATC
TTCAATCAGCTCGGTGTGGAAGGCGCAATCGTTCAGATGATCTCGCACGGCTTCGTGTCGGGCGCGATGTTCCTGAGCAT
CGGCGTGCTGTACGACCGTATGCATTCGCGCCAGATCGCCGATTACGGCGGTGTCGTCAACGTGATGCCGAAGTTCGCGG
CGTTCGTCATGCTGTTCTCCATGGCGAATTGCGGCCTGCCGGGCACCTCCGGTTTCGTCGGCGAGTTCATGGTGATTCTG
GCGGCAGTCCAGTACAACTTCTGGATCGCGGGCGGCGCGGCAGTCACGCTGATTCTCGGCGCGGCCTACACGTTGTGGAT
GTACAAGCGCGTGTACTTCGGCGCGATCGCCAACGATCACGTGAGGAGCCTGCTCGACATCAACCGTCGCGAGTTTTTCA
TGCTGGCAGTGCTCGCCGCACTGACGTTGTTCATGGGCCTGTATCCGAAGCCCTTTACCGATGTGATGCACGTATCCGTG
GAAAACCTCCTCTCCCACGTTGCGCAATCAAAGCTGCCGTTGCCTCAGTAA

Upstream 100 bases:

>100_bases
CCGGCTACATCTACCACTACGCGTTCGCCATGATTATCGGCATGTTGGGGCTCCTGACCCTGTTTGTAACGCTCGGCGGC
AAATAAGGCGAGGGACACTA

Downstream 100 bases:

>100_bases
CGCAGAGCGGAGGAATTTAAAGACCATGCAAAACGCCCCTATGACTGCTCTGTTGCCCGACGCGCTGGTGATGCTCGCCG
TTGTCGTCGCGTGGCTCAAC

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MHAYPILSIAIWLPILVGLLVLAIGSDRNPAPARWIALIGSVVSFLVTIPLITGFDSSTADLQFVEKANWIERFNITYHL
GVDGISMWFVVLTALITVIVVIAAWEVITKNVAQYLAAFLILSGIMVGVFSAADGMLFYVFFEATLIPMYIIIGVWGGAN
RVYAAFKFFLYTLMGSLLMLVALLYLYVQTGTFDLATWQHAQIAMTPQVLLFIAFFMAFAVKVPMWPVHTWLPDAHVEAP
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMKKLVAYSSIAHMGFVTLGFFI
FNQLGVEGAIVQMISHGFVSGAMFLSIGVLYDRMHSRQIADYGGVVNVMPKFAAFVMLFSMANCGLPGTSGFVGEFMVIL
AAVQYNFWIAGGAAVTLILGAAYTLWMYKRVYFGAIANDHVRSLLDINRREFFMLAVLAALTLFMGLYPKPFTDVMHVSV
ENLLSHVAQSKLPLPQ

Sequences:

>Translated_496_residues
MHAYPILSIAIWLPILVGLLVLAIGSDRNPAPARWIALIGSVVSFLVTIPLITGFDSSTADLQFVEKANWIERFNITYHL
GVDGISMWFVVLTALITVIVVIAAWEVITKNVAQYLAAFLILSGIMVGVFSAADGMLFYVFFEATLIPMYIIIGVWGGAN
RVYAAFKFFLYTLMGSLLMLVALLYLYVQTGTFDLATWQHAQIAMTPQVLLFIAFFMAFAVKVPMWPVHTWLPDAHVEAP
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMKKLVAYSSIAHMGFVTLGFFI
FNQLGVEGAIVQMISHGFVSGAMFLSIGVLYDRMHSRQIADYGGVVNVMPKFAAFVMLFSMANCGLPGTSGFVGEFMVIL
AAVQYNFWIAGGAAVTLILGAAYTLWMYKRVYFGAIANDHVRSLLDINRREFFMLAVLAALTLFMGLYPKPFTDVMHVSV
ENLLSHVAQSKLPLPQ
>Mature_496_residues
MHAYPILSIAIWLPILVGLLVLAIGSDRNPAPARWIALIGSVVSFLVTIPLITGFDSSTADLQFVEKANWIERFNITYHL
GVDGISMWFVVLTALITVIVVIAAWEVITKNVAQYLAAFLILSGIMVGVFSAADGMLFYVFFEATLIPMYIIIGVWGGAN
RVYAAFKFFLYTLMGSLLMLVALLYLYVQTGTFDLATWQHAQIAMTPQVLLFIAFFMAFAVKVPMWPVHTWLPDAHVEAP
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMKKLVAYSSIAHMGFVTLGFFI
FNQLGVEGAIVQMISHGFVSGAMFLSIGVLYDRMHSRQIADYGGVVNVMPKFAAFVMLFSMANCGLPGTSGFVGEFMVIL
AAVQYNFWIAGGAAVTLILGAAYTLWMYKRVYFGAIANDHVRSLLDINRREFFMLAVLAALTLFMGLYPKPFTDVMHVSV
ENLLSHVAQSKLPLPQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=363, Percent_Identity=34.1597796143251, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI251831117, Length=422, Percent_Identity=24.6445497630332, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1788613, Length=468, Percent_Identity=39.1025641025641, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI1788827, Length=379, Percent_Identity=23.2189973614776, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1788831, Length=316, Percent_Identity=24.6835443037975, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788829, Length=410, Percent_Identity=27.5609756097561, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI145693160, Length=450, Percent_Identity=23.7777777777778, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788614, Length=384, Percent_Identity=26.8229166666667, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI2367154, Length=244, Percent_Identity=23.7704918032787, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54386; Mature: 54386

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAYPILSIAIWLPILVGLLVLAIGSDRNPAPARWIALIGSVVSFLVTIPLITGFDSSTA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DLQFVEKANWIERFNITYHLGVDGISMWFVVLTALITVIVVIAAWEVITKNVAQYLAAFL
HHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILSGIMVGVFSAADGMLFYVFFEATLIPMYIIIGVWGGANRVYAAFKFFLYTLMGSLLML
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VALLYLYVQTGTFDLATWQHAQIAMTPQVLLFIAFFMAFAVKVPMWPVHTWLPDAHVEAP
HHHHHHHHHCCCEEECCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCC
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMK
CCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLVAYSSIAHMGFVTLGFFIFNQLGVEGAIVQMISHGFVSGAMFLSIGVLYDRMHSRQIA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYGGVVNVMPKFAAFVMLFSMANCGLPGTSGFVGEFMVILAAVQYNFWIAGGAAVTLILG
HHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEECHHHHHHHHH
AAYTLWMYKRVYFGAIANDHVRSLLDINRREFFMLAVLAALTLFMGLYPKPFTDVMHVSV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ENLLSHVAQSKLPLPQ
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHAYPILSIAIWLPILVGLLVLAIGSDRNPAPARWIALIGSVVSFLVTIPLITGFDSSTA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DLQFVEKANWIERFNITYHLGVDGISMWFVVLTALITVIVVIAAWEVITKNVAQYLAAFL
HHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILSGIMVGVFSAADGMLFYVFFEATLIPMYIIIGVWGGANRVYAAFKFFLYTLMGSLLML
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VALLYLYVQTGTFDLATWQHAQIAMTPQVLLFIAFFMAFAVKVPMWPVHTWLPDAHVEAP
HHHHHHHHHCCCEEECCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCC
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMK
CCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLVAYSSIAHMGFVTLGFFIFNQLGVEGAIVQMISHGFVSGAMFLSIGVLYDRMHSRQIA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYGGVVNVMPKFAAFVMLFSMANCGLPGTSGFVGEFMVILAAVQYNFWIAGGAAVTLILG
HHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEECHHHHHHHHH
AAYTLWMYKRVYFGAIANDHVRSLLDINRREFFMLAVLAALTLFMGLYPKPFTDVMHVSV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ENLLSHVAQSKLPLPQ
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]