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The map label for this gene is proP [H]

Identifier: 91782466

GI number: 91782466

Start: 1212919

End: 1214220

Strand: Direct

Name: proP [H]

Synonym: Bxe_A3365

Alternate gene names: 91782466

Gene position: 1212919-1214220 (Clockwise)

Preceding gene: 91782465

Following gene: 91782467

Centisome position: 24.77

GC content: 63.75

Gene sequence:

>1302_bases
ATGGAATCGATCGCTCTCGCTGTGAACGAAACGCAGCGTGTCGCGCAGCACAAGAAGGCGCTCGTTGCCGCTTGTGTCGG
CAACTTTATCGAGTACTACGACTTCGTGATCTACGGCTATTTTGCGTCCGTGATCGCGCGTCTGTTCTTTCCGGCCGACA
ACGAGGCCGCGAGCCTGCTGCTCACGTTCGCGGTGTTCGCCATCAGCTATGCGTCGCGACCGCTCGGCGGGCTGATCTTC
GGGCACCTCGGCGATCGCTACGGCCGCAAGACGCCGCTCGCCGTCGCGGTGATGCTGATCGCGTGCTCGACCACGGTGAT
CGGCCTGTTGCCCACCTATAGCGCGATCGGGATCGCGTCGCCGATCATTCTCACCGCCGCGCGGCTGGTGCAGGGCATTT
CTGTCGGCGGCGAGTATGGCGGCGCGACTTCGTTCATCGCCGAATATGCGCCGCCCGGCCGGCGCGGGTTCTATACCGGC
TGGCAGACCTTCACGATCGGCCTCGCGTTGCTGGTGGGCGGCGCGGTGGCGTCGATCATCACCGGAGCGCTGTCGGCCGA
AGACCTGCGCGCCTGGGGCTGGCGTCTGCCGTTTTTCGCGGGACTGCCGCTCGGTTTCGTCGGGCTCTATCTGCGGCTGA
AGCTGGAGGAAACGCCGCACTTCTCGTCGGTGCAACAGACTGCCGAGATCGAGCGCACGCCGCTCGTGACCGGATTGCGC
AGAGAGTGGAAGGCGATTCTGATCGGAATGGGGCTCATCGCCGCGCCCTCGGCGTGCATCTACATCTACTACATCTATTC
GCCGACGTATCTGTCCGTGGTGCTCGGTTTCAAGCTCGCGGACGCGCAGCGCGCCAACCTGTATAGCCTGATCTTCTATT
GCGCGCTACTGCCGGTCTTCGCGCACCTGAGCGACGTTGTGGGCCGTCGTCCGTTGATGCTCGTGAGTTCGCTCGCCATC
ATGCTCGTCACGTACCCGGCGTTTCATCTGCTCGATCCGCATGACTTCGGCAAGACGGTTGCGGCGCTATGCCTGATGAG
CCTCGCGTTCGCGCCGCATTCGGCCACGGCGCTATGCGCGATGGCCGAGATCATGCCGACCAGACTGCGCTACACCGGGC
TGTCGGTGAGCCTGAACATTCCGGTCACGCTGCTCGGCGGCACGGCGCCGTTCATTGCGACGTATCTCGTGTCGCGTACC
GGCGATCTGTATTCGCCGGCGTGGTTCGTGATCGGCGCAGGCCTGTGCACGTTGGTGGCCGCGTATGCGTATAAGGAGAC
GCTGCATAGTGGATTGCGGTGA

Upstream 100 bases:

>100_bases
GCGGGCAGCACCGTGGCGTGAACCTGAGCTGGCCTCGCCGTCAGAACAGGACGCCGGCCCACCCGACAACCGATAACAAA
CAAATATGGAGACTTGCGTG

Downstream 100 bases:

>100_bases
AGCGCCGGCGGCTTCAGCAGAGCTGTGAGAAATTGCCCATAATTGCGCGCAGTCTTCGAGCAACCGTTGGGACGTTTGAT
GAAAACAGCTTCTGTGGCAC

Product: major facilitator superfamily metabolite/H(+) symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MESIALAVNETQRVAQHKKALVAACVGNFIEYYDFVIYGYFASVIARLFFPADNEAASLLLTFAVFAISYASRPLGGLIF
GHLGDRYGRKTPLAVAVMLIACSTTVIGLLPTYSAIGIASPIILTAARLVQGISVGGEYGGATSFIAEYAPPGRRGFYTG
WQTFTIGLALLVGGAVASIITGALSAEDLRAWGWRLPFFAGLPLGFVGLYLRLKLEETPHFSSVQQTAEIERTPLVTGLR
REWKAILIGMGLIAAPSACIYIYYIYSPTYLSVVLGFKLADAQRANLYSLIFYCALLPVFAHLSDVVGRRPLMLVSSLAI
MLVTYPAFHLLDPHDFGKTVAALCLMSLAFAPHSATALCAMAEIMPTRLRYTGLSVSLNIPVTLLGGTAPFIATYLVSRT
GDLYSPAWFVIGAGLCTLVAAYAYKETLHSGLR

Sequences:

>Translated_433_residues
MESIALAVNETQRVAQHKKALVAACVGNFIEYYDFVIYGYFASVIARLFFPADNEAASLLLTFAVFAISYASRPLGGLIF
GHLGDRYGRKTPLAVAVMLIACSTTVIGLLPTYSAIGIASPIILTAARLVQGISVGGEYGGATSFIAEYAPPGRRGFYTG
WQTFTIGLALLVGGAVASIITGALSAEDLRAWGWRLPFFAGLPLGFVGLYLRLKLEETPHFSSVQQTAEIERTPLVTGLR
REWKAILIGMGLIAAPSACIYIYYIYSPTYLSVVLGFKLADAQRANLYSLIFYCALLPVFAHLSDVVGRRPLMLVSSLAI
MLVTYPAFHLLDPHDFGKTVAALCLMSLAFAPHSATALCAMAEIMPTRLRYTGLSVSLNIPVTLLGGTAPFIATYLVSRT
GDLYSPAWFVIGAGLCTLVAAYAYKETLHSGLR
>Mature_433_residues
MESIALAVNETQRVAQHKKALVAACVGNFIEYYDFVIYGYFASVIARLFFPADNEAASLLLTFAVFAISYASRPLGGLIF
GHLGDRYGRKTPLAVAVMLIACSTTVIGLLPTYSAIGIASPIILTAARLVQGISVGGEYGGATSFIAEYAPPGRRGFYTG
WQTFTIGLALLVGGAVASIITGALSAEDLRAWGWRLPFFAGLPLGFVGLYLRLKLEETPHFSSVQQTAEIERTPLVTGLR
REWKAILIGMGLIAAPSACIYIYYIYSPTYLSVVLGFKLADAQRANLYSLIFYCALLPVFAHLSDVVGRRPLMLVSSLAI
MLVTYPAFHLLDPHDFGKTVAALCLMSLAFAPHSATALCAMAEIMPTRLRYTGLSVSLNIPVTLLGGTAPFIATYLVSRT
GDLYSPAWFVIGAGLCTLVAAYAYKETLHSGLR

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=425, Percent_Identity=35.0588235294118, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1788292, Length=422, Percent_Identity=30.8056872037915, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1788942, Length=389, Percent_Identity=33.9331619537275, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1789941, Length=445, Percent_Identity=33.4831460674157, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI87082231, Length=238, Percent_Identity=28.9915966386555, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082404, Length=401, Percent_Identity=23.9401496259352, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46590; Mature: 46590

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIALAVNETQRVAQHKKALVAACVGNFIEYYDFVIYGYFASVIARLFFPADNEAASLL
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LTFAVFAISYASRPLGGLIFGHLGDRYGRKTPLAVAVMLIACSTTVIGLLPTYSAIGIAS
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIILTAARLVQGISVGGEYGGATSFIAEYAPPGRRGFYTGWQTFTIGLALLVGGAVASII
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TGALSAEDLRAWGWRLPFFAGLPLGFVGLYLRLKLEETPHFSSVQQTAEIERTPLVTGLR
HHHCCHHHHHHHCCCCCHHHCCHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCHHHHHH
REWKAILIGMGLIAAPSACIYIYYIYSPTYLSVVLGFKLADAQRANLYSLIFYCALLPVF
HHHHHHHHHHHHHHCCCEEEEEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AHLSDVVGRRPLMLVSSLAIMLVTYPAFHLLDPHDFGKTVAALCLMSLAFAPHSATALCA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
MAEIMPTRLRYTGLSVSLNIPVTLLGGTAPFIATYLVSRTGDLYSPAWFVIGAGLCTLVA
HHHHHHHHHEEECEEEEEECCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AYAYKETLHSGLR
HHHHHHHHHHCCC
>Mature Secondary Structure
MESIALAVNETQRVAQHKKALVAACVGNFIEYYDFVIYGYFASVIARLFFPADNEAASLL
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LTFAVFAISYASRPLGGLIFGHLGDRYGRKTPLAVAVMLIACSTTVIGLLPTYSAIGIAS
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIILTAARLVQGISVGGEYGGATSFIAEYAPPGRRGFYTGWQTFTIGLALLVGGAVASII
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TGALSAEDLRAWGWRLPFFAGLPLGFVGLYLRLKLEETPHFSSVQQTAEIERTPLVTGLR
HHHCCHHHHHHHCCCCCHHHCCHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCHHHHHH
REWKAILIGMGLIAAPSACIYIYYIYSPTYLSVVLGFKLADAQRANLYSLIFYCALLPVF
HHHHHHHHHHHHHHCCCEEEEEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AHLSDVVGRRPLMLVSSLAIMLVTYPAFHLLDPHDFGKTVAALCLMSLAFAPHSATALCA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
MAEIMPTRLRYTGLSVSLNIPVTLLGGTAPFIATYLVSRTGDLYSPAWFVIGAGLCTLVA
HHHHHHHHHEEECEEEEEECCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AYAYKETLHSGLR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]