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The map label for this gene is sspA [H]

Identifier: 91782385

GI number: 91782385

Start: 1124279

End: 1124938

Strand: Reverse

Name: sspA [H]

Synonym: Bxe_A3446

Alternate gene names: 91782385

Gene position: 1124938-1124279 (Counterclockwise)

Preceding gene: 91782386

Following gene: 91782384

Centisome position: 22.98

GC content: 66.67

Gene sequence:

>660_bases
ATGCCCCTGACGCTCTATGCCCATCCGTTCTCCTCTTACTGCCAGAAGGTCCTGACCGCGCTCTACGAGAACGGCACACC
ATTCGAGCTACGCCTGCTGGCGCACGACGACCCGCAAGTCATGGCCGAATTCGCCGCGCTCTGGCCGATCAAGCGGTTTC
CGCTGCTGGTGGACAGCGGCCGGACCGTGTTGGAGTCGAGCATCATCATCGAATATCTGGGCCTCTATCACCCCGGGCCG
GTACCGCTGCTGCCGGCCGACGCCCGCGCCGCGCTCGAAGTGCGCGGCATGGACCGCTTCTTCGACAACTACATCTCGAC
TCCGCAGCAAAAGATCGTCTACGACAGCATGCGCCCCGAGCCGCAACGGGACCCGCACGCCGTAGCCGACGCGCGCGCCA
TGCTCGACACCGCGTATGGCTGGCTGGACAAGGTGATGGCAGAACGCGAATGGGCCGCCGGCGACACCTTCAGCCTCGCC
GACTGCGGCGCCGCGCCGTTCCTTTTCTATGCCGACTGGACGCACGCCATCGGCGCGGCCTTTCCGCATGTGCTGGCTTA
CCGCAAGCGGCTGTTGGCGCGGCCGTCGTTCGCACGCGCGGTGGACGAAGCGCGCCCCTATCGCGCGTTTTTCCCGCTCG
GCGCGCCGGACCGGGATTGA

Upstream 100 bases:

>100_bases
CGTCGCTGTGTCGATTCGCGCCGCCGCCATTCGTCGTGAGGTTGACGGCCCCGCGCCGCCGTCGCGCACCCAACCGCCAC
AATCGACCAAGGTGACCACG

Downstream 100 bases:

>100_bases
GCGCAGCCGCACGGCCGCGGCTGGGACCGCGGCGGCTGCTGCGGCGGCCACGGGCAAACCGCAACACGAGCGCTGGCAAG
CCGCCCATTCTTACCCTTTG

Product: putative glutathione S-transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MPLTLYAHPFSSYCQKVLTALYENGTPFELRLLAHDDPQVMAEFAALWPIKRFPLLVDSGRTVLESSIIIEYLGLYHPGP
VPLLPADARAALEVRGMDRFFDNYISTPQQKIVYDSMRPEPQRDPHAVADARAMLDTAYGWLDKVMAEREWAAGDTFSLA
DCGAAPFLFYADWTHAIGAAFPHVLAYRKRLLARPSFARAVDEARPYRAFFPLGAPDRD

Sequences:

>Translated_219_residues
MPLTLYAHPFSSYCQKVLTALYENGTPFELRLLAHDDPQVMAEFAALWPIKRFPLLVDSGRTVLESSIIIEYLGLYHPGP
VPLLPADARAALEVRGMDRFFDNYISTPQQKIVYDSMRPEPQRDPHAVADARAMLDTAYGWLDKVMAEREWAAGDTFSLA
DCGAAPFLFYADWTHAIGAAFPHVLAYRKRLLARPSFARAVDEARPYRAFFPLGAPDRD
>Mature_218_residues
PLTLYAHPFSSYCQKVLTALYENGTPFELRLLAHDDPQVMAEFAALWPIKRFPLLVDSGRTVLESSIIIEYLGLYHPGPV
PLLPADARAALEVRGMDRFFDNYISTPQQKIVYDSMRPEPQRDPHAVADARAMLDTAYGWLDKVMAEREWAAGDTFSLAD
CGAAPFLFYADWTHAIGAAFPHVLAYRKRLLARPSFARAVDEARPYRAFFPLGAPDRD

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1789624, Length=204, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17864598, Length=165, Percent_Identity=29.0909090909091, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 24678; Mature: 24547

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTLYAHPFSSYCQKVLTALYENGTPFELRLLAHDDPQVMAEFAALWPIKRFPLLVDSG
CCEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCHHHCCEEECCC
RTVLESSIIIEYLGLYHPGPVPLLPADARAALEVRGMDRFFDNYISTPQQKIVYDSMRPE
CHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
PQRDPHAVADARAMLDTAYGWLDKVMAEREWAAGDTFSLADCGAAPFLFYADWTHAIGAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEEEECCHHHHHHHH
FPHVLAYRKRLLARPSFARAVDEARPYRAFFPLGAPDRD
HHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCCCCC
>Mature Secondary Structure 
PLTLYAHPFSSYCQKVLTALYENGTPFELRLLAHDDPQVMAEFAALWPIKRFPLLVDSG
CEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCHHHCCEEECCC
RTVLESSIIIEYLGLYHPGPVPLLPADARAALEVRGMDRFFDNYISTPQQKIVYDSMRPE
CHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
PQRDPHAVADARAMLDTAYGWLDKVMAEREWAAGDTFSLADCGAAPFLFYADWTHAIGAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEEEECCHHHHHHHH
FPHVLAYRKRLLARPSFARAVDEARPYRAFFPLGAPDRD
HHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]