Click here to switch to the map view.

The map label for this gene is glk

Identifier: 91782377

GI number: 91782377

Start: 1113850

End: 1115766

Strand: Reverse

Name: glk

Synonym: Bxe_A3454

Alternate gene names: 91782377

Gene position: 1115766-1113850 (Counterclockwise)

Preceding gene: 91782378

Following gene: 91782375

Centisome position: 22.79

GC content: 65.05

Gene sequence:

>1917_bases
ATGTCTACTGGTGTGCAAACTAAGGCTGCTCCGGGCGCGGGCCAGCACGCCGATGGACCCAGGCTGCTGGCCGATATCGG
CGGCACCAACGCGCGCTTCGCGCTGGAGACGAGTCCGGGCGAAATCGGTTCGGTGAAGGTCTACCCGTGCGCGGACTATC
CGGGTGTTGCCGAAGTCATCAAGAGGTATCTGAAGGACACTAAAATCGGCCGCGTGAATCACGCGGCGATTGCGATTGCG
AACCCGGTCGACGGCGATCAGGTGAGCATGACGAATCACGACTGGAGCTTTTCGATCGAAGCCACGCGCCGCGCGCTCGG
CTTCGACACGCTGCTGGTGGTGAACGACTTCACCGCGCTGGCTATGGCGCTGCCCGGCCTCACCGACGCGCAGCGCGTGC
AGGTGGGCGGCGGCACGCGCCGGCCGAACAGCGTGATCGGCCTGCTCGGGCCGGGCACCGGCATGGGCGTATCCGGCCTG
ATTCCCGCCGACGACCGCTGGATCGCGCTCGGCAGCGAAGGCGGCCACGCCACCTTCGCGCCGGCCGACGAACGCGAAGA
CATCGTGCTGCAATACGCGCGCAAGAAGTGGTCGCACGTGTCGTTCGAACGCGTGGCCGCGGGTCCCGGCATCGAAGTGA
TCTACCGTGCGCTCGCGGGCCGCGACAAGAAGCGCGTGGCGGCCAACGTCGACACGATCGAGATCGTCAAGCGCGCAATG
GAAGGCGAGCCGCTCGCGGCCGAATCCGTCGACGTGTTCTGCGGCATTCTCGGCACCTTCGCCGGCAATATCGCGGTGAC
GCTCGGCGCGCTGGGCGGCATCTATATCGGCGGCGGCGTGGTGCCGCGGCTCGGTGAGCTCTTCGCGCGCTCGTCGTTTC
GCAAGCGCTTCGAAGCCAAGGGCCGCTTCGAGGCCTACCTGCAGAATGTGCCGACATATGTGATTACCGCCGAATATCCC
GCGTTCCTCGGCGTCTCGGCAATTCTCGCCGAACAGTTGTCGAACCGCGCGGGCGGCAGTTCATCGGCAGTGTTCGAGCG
GATTCGCCAGATGCGCGATGCGTTGACGCCGGCCGAGCGCCGCGTGGCCGATCTCGCGCTGAACCATCCGCGTTCGATCA
TCAACGATCCGATCGTCGATATCGCGCGCAAGGCCGACGTGAGTCAGCCTACCGTGATCCGCTTCTGCCGTTCGCTCGGC
TGCCAGGGCCTGTCCGATTTCAAGCTGAAGCTGGCAACGGGCTTGACCGGCACGATTCCCGTGAGCCACAGCCAGGTGCA
TCTGGGCGACACGGCCACGGACTTCGGCGCGAAGGTGCTGGACAACACCGTGTCCGCGATCCTGCAGTTGCGCGAGCATC
TGAACTTCGAGCATGTCGAGCGGGCCATTGATCTGCTGAATGGCGCGCGGCGCATCGAGTTCTATGGCCTCGGCAATTCG
AACATCGTCGCGCAGGACGCGCACTACAAGTTCTTCCGCTTCGGCATTCCGACGATTGCGTATGGCGACCTGTACATGCA
GGCGGCGTCGGCGGCCTTGCTCGGCAAGGGCGATGTGATCGTGGCGGTGTCCAAGTCGGGGCGCGCGCCCGAACTGCTGC
GCGTGCTGGATGTGGCGATGCAGGCCGGCGCCAAGGTGATCGCGATCACGTCGAGCAATACGCCTTTGGCCAAGCGGGCC
ACGGTTGCGCTCGAAACCGATCACATTGAAATTCGCGAGTCGCAGTTGTCGATGATTTCGCGGATTCTGCATCTCGTGAT
GATCGATATTCTTGCTGTCGGGGTAGCGATCCGCCGAGCGGTGCCGAGCGCCGATGTGGCGGAGACGGTCGCCAAGGCGC
GCCAGGGCGCCGACGATGATGCGACTGCCGTGCTGGATTGGCTGAGCCACGGCGCGGCTTCTTCGGCGCGGGATTGA

Upstream 100 bases:

>100_bases
ATTGCGGGACCGCGCAAGATGGACGTGCTGAATGCCGCGTCCGCTTCGCTACAAAAAAATGCCATCTCACAGTTGGCAAA
CGACAAGGGAGTGAGACTCG

Downstream 100 bases:

>100_bases
TGTTGAACGGCCCGCGCCGTGCGGCGTGGTGGTTTGCCTGCGGTGCCGGCCTTTCCTTGACGTTACGGCGGCTCAAGCGT
GTTGCCCCGTGCGGGCTACG

Product: bifunctional glucokinase/RpiR family transcriptional regulator

Products: NA

Alternate protein names: Glucokinase; Glucose kinase; Putative HTH-type transcriptional regulator

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MSTGVQTKAAPGAGQHADGPRLLADIGGTNARFALETSPGEIGSVKVYPCADYPGVAEVIKRYLKDTKIGRVNHAAIAIA
NPVDGDQVSMTNHDWSFSIEATRRALGFDTLLVVNDFTALAMALPGLTDAQRVQVGGGTRRPNSVIGLLGPGTGMGVSGL
IPADDRWIALGSEGGHATFAPADEREDIVLQYARKKWSHVSFERVAAGPGIEVIYRALAGRDKKRVAANVDTIEIVKRAM
EGEPLAAESVDVFCGILGTFAGNIAVTLGALGGIYIGGGVVPRLGELFARSSFRKRFEAKGRFEAYLQNVPTYVITAEYP
AFLGVSAILAEQLSNRAGGSSSAVFERIRQMRDALTPAERRVADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLG
CQGLSDFKLKLATGLTGTIPVSHSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVERAIDLLNGARRIEFYGLGNS
NIVAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLDVAMQAGAKVIAITSSNTPLAKRA
TVALETDHIEIRESQLSMISRILHLVMIDILAVGVAIRRAVPSADVAETVAKARQGADDDATAVLDWLSHGAASSARD

Sequences:

>Translated_638_residues
MSTGVQTKAAPGAGQHADGPRLLADIGGTNARFALETSPGEIGSVKVYPCADYPGVAEVIKRYLKDTKIGRVNHAAIAIA
NPVDGDQVSMTNHDWSFSIEATRRALGFDTLLVVNDFTALAMALPGLTDAQRVQVGGGTRRPNSVIGLLGPGTGMGVSGL
IPADDRWIALGSEGGHATFAPADEREDIVLQYARKKWSHVSFERVAAGPGIEVIYRALAGRDKKRVAANVDTIEIVKRAM
EGEPLAAESVDVFCGILGTFAGNIAVTLGALGGIYIGGGVVPRLGELFARSSFRKRFEAKGRFEAYLQNVPTYVITAEYP
AFLGVSAILAEQLSNRAGGSSSAVFERIRQMRDALTPAERRVADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLG
CQGLSDFKLKLATGLTGTIPVSHSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVERAIDLLNGARRIEFYGLGNS
NIVAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLDVAMQAGAKVIAITSSNTPLAKRA
TVALETDHIEIRESQLSMISRILHLVMIDILAVGVAIRRAVPSADVAETVAKARQGADDDATAVLDWLSHGAASSARD
>Mature_637_residues
STGVQTKAAPGAGQHADGPRLLADIGGTNARFALETSPGEIGSVKVYPCADYPGVAEVIKRYLKDTKIGRVNHAAIAIAN
PVDGDQVSMTNHDWSFSIEATRRALGFDTLLVVNDFTALAMALPGLTDAQRVQVGGGTRRPNSVIGLLGPGTGMGVSGLI
PADDRWIALGSEGGHATFAPADEREDIVLQYARKKWSHVSFERVAAGPGIEVIYRALAGRDKKRVAANVDTIEIVKRAME
GEPLAAESVDVFCGILGTFAGNIAVTLGALGGIYIGGGVVPRLGELFARSSFRKRFEAKGRFEAYLQNVPTYVITAEYPA
FLGVSAILAEQLSNRAGGSSSAVFERIRQMRDALTPAERRVADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLGC
QGLSDFKLKLATGLTGTIPVSHSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVERAIDLLNGARRIEFYGLGNSN
IVAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLDVAMQAGAKVIAITSSNTPLAKRAT
VALETDHIEIRESQLSMISRILHLVMIDILAVGVAIRRAVPSADVAETVAKARQGADDDATAVLDWLSHGAASSARD

Specific function: Not Highly Important In E.Coli As Glucose Is Transported Into The Cell By The Pts System Already As Glucose 6-Phosphate. [C]

COG id: COG0837

COG function: function code G; Glucokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain

Homologues:

Organism=Escherichia coli, GI1788732, Length=316, Percent_Identity=43.3544303797468, Blast_Score=268, Evalue=9e-73,
Organism=Escherichia coli, GI1788159, Length=270, Percent_Identity=37.037037037037, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI87082369, Length=251, Percent_Identity=29.0836653386454, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788767, Length=249, Percent_Identity=26.5060240963855, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87082130, Length=226, Percent_Identity=28.7610619469027, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLK_BURXL (Q143F8)

Other databases:

- EMBL:   CP000270
- RefSeq:   YP_557583.1
- ProteinModelPortal:   Q143F8
- GeneID:   4001984
- GenomeReviews:   CP000270_GR
- KEGG:   bxe:Bxe_A3454
- HOGENOM:   HBG367942
- OMA:   HYLQNIP
- ProtClustDB:   PRK14101
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00524
- InterPro:   IPR003836
- InterPro:   IPR000843
- InterPro:   IPR000281
- InterPro:   IPR001347
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- TIGRFAMs:   TIGR00749

Pfam domain/function: PF02685 Glucokinase; PF01418 HTH_6; PF01380 SIS

EC number: =2.7.1.2

Molecular weight: Translated: 67858; Mature: 67727

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS00356 HTH_LACI_1; PS51071 HTH_RPIR; PS51464 SIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGVQTKAAPGAGQHADGPRLLADIGGTNARFALETSPGEIGSVKVYPCADYPGVAEVI
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCEEEEECCCCCCHHHHH
KRYLKDTKIGRVNHAAIAIANPVDGDQVSMTNHDWSFSIEATRRALGFDTLLVVNDFTAL
HHHHHHCCCCCCCCEEEEEECCCCCCEEEECCCCCEEEHHHHHHHHCCCEEEEHHHHHHH
AMALPGLTDAQRVQVGGGTRRPNSVIGLLGPGTGMGVSGLIPADDRWIALGSEGGHATFA
HHHCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
PADEREDIVLQYARKKWSHVSFERVAAGPGIEVIYRALAGRDKKRVAANVDTIEIVKRAM
CCCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHCCCHHHHHHHHHH
EGEPLAAESVDVFCGILGTFAGNIAVTLGALGGIYIGGGVVPRLGELFARSSFRKRFEAK
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHCC
GRFEAYLQNVPTYVITAEYPAFLGVSAILAEQLSNRAGGSSSAVFERIRQMRDALTPAER
CHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHH
RVADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLGCQGLSDFKLKLATGLTGTIP
HHHHHHHCCCHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCEEEE
VSHSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVERAIDLLNGARRIEFYGLGNS
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCC
NIVAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLDVAM
CEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
QAGAKVIAITSSNTPLAKRATVALETDHIEIRESQLSMISRILHLVMIDILAVGVAIRRA
HCCCEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VPSADVAETVAKARQGADDDATAVLDWLSHGAASSARD
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
STGVQTKAAPGAGQHADGPRLLADIGGTNARFALETSPGEIGSVKVYPCADYPGVAEVI
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCEEEEECCCCCCHHHHH
KRYLKDTKIGRVNHAAIAIANPVDGDQVSMTNHDWSFSIEATRRALGFDTLLVVNDFTAL
HHHHHHCCCCCCCCEEEEEECCCCCCEEEECCCCCEEEHHHHHHHHCCCEEEEHHHHHHH
AMALPGLTDAQRVQVGGGTRRPNSVIGLLGPGTGMGVSGLIPADDRWIALGSEGGHATFA
HHHCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
PADEREDIVLQYARKKWSHVSFERVAAGPGIEVIYRALAGRDKKRVAANVDTIEIVKRAM
CCCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHCCCHHHHHHHHHH
EGEPLAAESVDVFCGILGTFAGNIAVTLGALGGIYIGGGVVPRLGELFARSSFRKRFEAK
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHCC
GRFEAYLQNVPTYVITAEYPAFLGVSAILAEQLSNRAGGSSSAVFERIRQMRDALTPAER
CHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHH
RVADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLGCQGLSDFKLKLATGLTGTIP
HHHHHHHCCCHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCEEEE
VSHSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVERAIDLLNGARRIEFYGLGNS
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCC
NIVAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLDVAM
CEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
QAGAKVIAITSSNTPLAKRATVALETDHIEIRESQLSMISRILHLVMIDILAVGVAIRRA
HCCCEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VPSADVAETVAKARQGADDDATAVLDWLSHGAASSARD
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA