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The map label for this gene is metG

Identifier: 91782348

GI number: 91782348

Start: 1081529

End: 1083661

Strand: Reverse

Name: metG

Synonym: Bxe_A3483

Alternate gene names: 91782348

Gene position: 1083661-1081529 (Counterclockwise)

Preceding gene: 91782349

Following gene: 91782345

Centisome position: 22.13

GC content: 62.4

Gene sequence:

>2133_bases
ATGTCAGCACCCGCAACCGACCTCTCCGCAGGCGCGCCGTCAGGCCGTCGCCAGATTCTCGTCACGTCGGCCCTTCCCTA
TGCGAACGGGCAAATCCATATTGGCCATCTGGTCGAATATATCCAGACGGACATCTGGGTCCGGACGTTGCGAATGCACG
GACACGAGGTCTACTACGTGGGCGCCGACGACACGCACGGCACGCCCGTCATGCTGCGCGCGGAGAAAGAAGGTCTCACG
CCGAAGCAGCTGATCGACCGCGTCTGGCAGGAACACAAGCGCGACTTCGACAGCTTCGGGATTTCGTTCGACAATTACTA
TTCGACCGACTCCGAAGAGAACCGCGTTCTCAGCGAAAACGTCTATCTGGCGCTAAAGGAAGCAGGCCTGATCGACGCGC
GCGACATCGAACAGGCGTATGACCCAGTCAAGGAAATGTTCCTGCCGGATCGCTTCATCAAGGGCGAATGTCCGAAATGC
GGCGCGAAAGACCAGTACGGCGACAGCTGCGAAGTGTGCGGTTCAACGTATCTGCCCACCGAGCTGATCAATCCGTACTC
GGTCGTCTCGGGCGCCACGCCGGTTCGCAAGACCTCGACACACTATTTCTTCCGTCTCTCCGATCCGCGTTGCGAAAATT
TCCTGCGCGCATGGGTGGGCGGTCTGGCGCAGCCCGAAGCCACCAACAAGATGCGCGAATGGCTCGGCGACGCCGGCGAA
GCCAAGCTCGCCGACTGGGACATCTCGCGCGACGCGCCGTATTTCGGTTTCGAAATTCCGGGCGCGCCGGGCAAGTATTT
CTATGTGTGGCTGGACGCGCCGGTCGGCTACTACGCGAGCTTCAAGAACCTCGCCGAAAAACGCGGCCTCGATTTCGACG
CGTGGGTTCGCAAGGGTTCGAAGGCCGAGCAGTATCACTTCATCGGCAAGGACATTCTGTATTTCCACACGCTGTTCTGG
CCGGCCATGCTCGAGTTTTCGGGCCACCGCACGCCGACCAACGTGTTCGCGCACGGTTTCCTGACCGTGGACGGCGCGAA
GATGTCGAAGTCGCGCGGCACCTTCATCACCGCGCAGAGCGTGATCGAGACGGGCCTGAATCCGGAATGGCTGCGCTATT
ACTTCGCGGCCAAGCTGAACAGCACGATGGAAGACCTCGACCTGAACCTCGACGACTTCCAGGCGCGCGTCAATAGCGAT
CTGGTCGGCAAATACGTGAACATCGCGAGCCGCGCGGCCGGCTTCCTGATCAAGCGCTTCGACGGCCGCGTGCAGGACAG
CGCGATGCAGCATCCGCTGCTCGCCTCGCTGCGCGCCGCCGTGCCGCAAATCGCCGCGAACTACGAAGCCCGCGAGTACA
ACCGCGCGCTGCGCCAGACCATGGAACTGGCCGACGCGGTGAACGCATACGTCGACACCGCCAAGCCGTGGGATCAGGCC
AAAGACCCGGCGAACGGCGTCGCGCTGCATGAAACGTGCAGCGTGAGCATCGAGGCGTTCCGCCTGCTGTCGCTGGCGCT
GAAGCCAGTGCTGCCGAAGCTCGCCGAAGCGGTCGAAGGCTTCCTCGCCATCCAACCGCTGGTGTGGGCGGACGCCAACG
TGCCGCTCAGTTCCACCCGGCCAATCAACGCGTACAAGCATCTGATGACACGCGTCGATCCGAAACAGATCGAAGCGCTG
CTCGCCGCCAATCGCGACTCGCTGCAAGCCACGCCGGAAGCAGCCGCGCCGGCCGACGCCAAGGGCAAGTCGAAGGCAGC
GAAAGCCGCCGCCGGCAAGGACGAAACACCCGGCATCATCTCGATCGACGACTTCGCGAAGATCGATTTGCGCATTGCGA
AGATCGTCGACTGCAAGGCGGTGGAAGGTTCGGACAAGCTGCTGCAACTCACGCTCGACGTCGGCGAAGAGAAAACCCGC
AACGTGTTTTCCGGTATCAAGTCGGCGTATCAGCCGGAGCAGCTGGTCGGCAAGCTGACGGTCATGGTCGCCAACCTCGC
GCCGCGCAAGATGAAGTTCGGCCTGTCCGAAGGCATGGTGCTGGCCGCCTCCGCCACCGACGAGAAAGCCGAACCGGGCC
TTTACGTGCTCGAACCGGACAGCGGCGCGAAGCCGGGCATGCGCGTGAAGTAA

Upstream 100 bases:

>100_bases
CCGCAACGACTCGCGCCGCGCCCCACGCGGCCCGCCCCGCGCCGGGCGCCTGCCCTGCTAGAATCAACGTTTCGTCCCAC
CGCAGGCTCCCACCATCCTT

Downstream 100 bases:

>100_bases
AGCGCGCGGCGGCTTGCGCTGCAGGCGCGAATAAAAACGGCACGCTCTTAAAAGTCGTGCCGTTTTTTTTGCTGTGCCGT
TTTCTGCCGCGCTTTTTTTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MSAPATDLSAGAPSGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYVGADDTHGTPVMLRAEKEGLT
PKQLIDRVWQEHKRDFDSFGISFDNYYSTDSEENRVLSENVYLALKEAGLIDARDIEQAYDPVKEMFLPDRFIKGECPKC
GAKDQYGDSCEVCGSTYLPTELINPYSVVSGATPVRKTSTHYFFRLSDPRCENFLRAWVGGLAQPEATNKMREWLGDAGE
AKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLAEKRGLDFDAWVRKGSKAEQYHFIGKDILYFHTLFW
PAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSVIETGLNPEWLRYYFAAKLNSTMEDLDLNLDDFQARVNSD
LVGKYVNIASRAAGFLIKRFDGRVQDSAMQHPLLASLRAAVPQIAANYEAREYNRALRQTMELADAVNAYVDTAKPWDQA
KDPANGVALHETCSVSIEAFRLLSLALKPVLPKLAEAVEGFLAIQPLVWADANVPLSSTRPINAYKHLMTRVDPKQIEAL
LAANRDSLQATPEAAAPADAKGKSKAAKAAAGKDETPGIISIDDFAKIDLRIAKIVDCKAVEGSDKLLQLTLDVGEEKTR
NVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYVLEPDSGAKPGMRVK

Sequences:

>Translated_710_residues
MSAPATDLSAGAPSGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYVGADDTHGTPVMLRAEKEGLT
PKQLIDRVWQEHKRDFDSFGISFDNYYSTDSEENRVLSENVYLALKEAGLIDARDIEQAYDPVKEMFLPDRFIKGECPKC
GAKDQYGDSCEVCGSTYLPTELINPYSVVSGATPVRKTSTHYFFRLSDPRCENFLRAWVGGLAQPEATNKMREWLGDAGE
AKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLAEKRGLDFDAWVRKGSKAEQYHFIGKDILYFHTLFW
PAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSVIETGLNPEWLRYYFAAKLNSTMEDLDLNLDDFQARVNSD
LVGKYVNIASRAAGFLIKRFDGRVQDSAMQHPLLASLRAAVPQIAANYEAREYNRALRQTMELADAVNAYVDTAKPWDQA
KDPANGVALHETCSVSIEAFRLLSLALKPVLPKLAEAVEGFLAIQPLVWADANVPLSSTRPINAYKHLMTRVDPKQIEAL
LAANRDSLQATPEAAAPADAKGKSKAAKAAAGKDETPGIISIDDFAKIDLRIAKIVDCKAVEGSDKLLQLTLDVGEEKTR
NVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYVLEPDSGAKPGMRVK
>Mature_709_residues
SAPATDLSAGAPSGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYVGADDTHGTPVMLRAEKEGLTP
KQLIDRVWQEHKRDFDSFGISFDNYYSTDSEENRVLSENVYLALKEAGLIDARDIEQAYDPVKEMFLPDRFIKGECPKCG
AKDQYGDSCEVCGSTYLPTELINPYSVVSGATPVRKTSTHYFFRLSDPRCENFLRAWVGGLAQPEATNKMREWLGDAGEA
KLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLAEKRGLDFDAWVRKGSKAEQYHFIGKDILYFHTLFWP
AMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSVIETGLNPEWLRYYFAAKLNSTMEDLDLNLDDFQARVNSDL
VGKYVNIASRAAGFLIKRFDGRVQDSAMQHPLLASLRAAVPQIAANYEAREYNRALRQTMELADAVNAYVDTAKPWDQAK
DPANGVALHETCSVSIEAFRLLSLALKPVLPKLAEAVEGFLAIQPLVWADANVPLSSTRPINAYKHLMTRVDPKQIEALL
AANRDSLQATPEAAAPADAKGKSKAAKAAAGKDETPGIISIDDFAKIDLRIAKIVDCKAVEGSDKLLQLTLDVGEEKTRN
VFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYVLEPDSGAKPGMRVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=565, Percent_Identity=26.9026548672566, Blast_Score=219, Evalue=8e-57,
Organism=Homo sapiens, GI24308436, Length=547, Percent_Identity=26.508226691042, Blast_Score=153, Evalue=7e-37,
Organism=Homo sapiens, GI4507947, Length=148, Percent_Identity=33.1081081081081, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788432, Length=701, Percent_Identity=54.7788873038516, Blast_Score=751, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=110, Percent_Identity=37.2727272727273, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17540794, Length=656, Percent_Identity=27.1341463414634, Blast_Score=230, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI212645096, Length=535, Percent_Identity=23.5514018691589, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71992781, Length=275, Percent_Identity=26.1818181818182, Blast_Score=100, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6321704, Length=566, Percent_Identity=27.208480565371, Blast_Score=217, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6321610, Length=548, Percent_Identity=24.2700729927007, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24655311, Length=568, Percent_Identity=29.4014084507042, Blast_Score=227, Evalue=3e-59,
Organism=Drosophila melanogaster, GI21358483, Length=535, Percent_Identity=27.1028037383178, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_BURXL (Q143I7)

Other databases:

- EMBL:   CP000270
- RefSeq:   YP_557554.1
- ProteinModelPortal:   Q143I7
- GeneID:   4002417
- GenomeReviews:   CP000270_GR
- KEGG:   bxe:Bxe_A3483
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 78510; Mature: 78379

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 350-350

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPATDLSAGAPSGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYV
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEE
GADDTHGTPVMLRAEKEGLTPKQLIDRVWQEHKRDFDSFGISFDNYYSTDSEENRVLSEN
ECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCEECCC
VYLALKEAGLIDARDIEQAYDPVKEMFLPDRFIKGECPKCGAKDQYGDSCEVCGSTYLPT
EEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHHCCCCCCCH
ELINPYSVVSGATPVRKTSTHYFFRLSDPRCENFLRAWVGGLAQPEATNKMREWLGDAGE
HHCCCHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
AKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLAEKRGLDFDAWVRKGS
CEEECCCCCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHCCC
KAEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCHHCCCCCCEEEHHH
VIETGLNPEWLRYYFAAKLNSTMEDLDLNLDDFQARVNSDLVGKYVNIASRAAGFLIKRF
HHHCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
DGRVQDSAMQHPLLASLRAAVPQIAANYEAREYNRALRQTMELADAVNAYVDTAKPWDQA
CCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHC
KDPANGVALHETCSVSIEAFRLLSLALKPVLPKLAEAVEGFLAIQPLVWADANVPLSSTR
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
PINAYKHLMTRVDPKQIEALLAANRDSLQATPEAAAPADAKGKSKAAKAAAGKDETPGII
CHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEE
SIDDFAKIDLRIAKIVDCKAVEGSDKLLQLTLDVGEEKTRNVFSGIKSAYQPEQLVGKLT
EECCCHHHHEEEEEEEECEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHH
VMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYVLEPDSGAKPGMRVK
HHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SAPATDLSAGAPSGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYV
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEE
GADDTHGTPVMLRAEKEGLTPKQLIDRVWQEHKRDFDSFGISFDNYYSTDSEENRVLSEN
ECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCEECCC
VYLALKEAGLIDARDIEQAYDPVKEMFLPDRFIKGECPKCGAKDQYGDSCEVCGSTYLPT
EEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHHCCCCCCCH
ELINPYSVVSGATPVRKTSTHYFFRLSDPRCENFLRAWVGGLAQPEATNKMREWLGDAGE
HHCCCHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
AKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLAEKRGLDFDAWVRKGS
CEEECCCCCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHCCC
KAEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCHHCCCCCCEEEHHH
VIETGLNPEWLRYYFAAKLNSTMEDLDLNLDDFQARVNSDLVGKYVNIASRAAGFLIKRF
HHHCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
DGRVQDSAMQHPLLASLRAAVPQIAANYEAREYNRALRQTMELADAVNAYVDTAKPWDQA
CCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHC
KDPANGVALHETCSVSIEAFRLLSLALKPVLPKLAEAVEGFLAIQPLVWADANVPLSSTR
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
PINAYKHLMTRVDPKQIEALLAANRDSLQATPEAAAPADAKGKSKAAKAAAGKDETPGII
CHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEE
SIDDFAKIDLRIAKIVDCKAVEGSDKLLQLTLDVGEEKTRNVFSGIKSAYQPEQLVGKLT
EECCCHHHHEEEEEEEECEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHH
VMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYVLEPDSGAKPGMRVK
HHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA