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The map label for this gene is lutR [H]

Identifier: 91782299

GI number: 91782299

Start: 1028000

End: 1028746

Strand: Direct

Name: lutR [H]

Synonym: Bxe_A3534

Alternate gene names: 91782299

Gene position: 1028000-1028746 (Clockwise)

Preceding gene: 91782298

Following gene: 91782301

Centisome position: 21.0

GC content: 61.18

Gene sequence:

>747_bases
ATGTCCCCCGTTGTCCGCGAATCGACTTCACGCTCTGAACTCGTCGGGAAGATGCTGGCGTTTTTCAAGGAACGTGGCTA
CGAAAGCGGCGAGCGTTTGCCGTCCGAGCGCGCGCTCGCCGAGCGTTTCGGCGTGGGGCGCAACGCACTGCGGGAAGCGC
TCTCTACGCTCAGCGCCCTTCGCGTGATCGAATCGCGGCCGAATTCGGGCATCTATCTTCGCCGCATGTCGACAGACAGC
AGCTTCGAGACGATCGTGCTGCTGACCGAGATGGGCTCCGAGCCGTCGATGAAGGAGGTGAAGGAGACCCTCGAAGTGCG
CTGGGCACTCGAAATCCGTGGCGTGCAACTGGCATGCGAGCGCCGCACGGAAGCGGATCTCGACAGTCTCGCATCGGTGA
TCGACGCGACTCGGGCAGTCCTGTCGAACAAGGGCAACATTGCGGCTCAAGACACAGCGTTCCACATGGCGCTTGCACGT
TGCGCGCATAACGAGATGCTCGTGCGGCTACTCAACTCGTTCTACCGCATTTCCGAAAGCCGGCGTTTTGCGCTGTTCGC
CGATTCCGGTCGTGGCACGATTTCGGCCGCTCAGCACGAGAGCCTTTACGAAGCAGTGAAGAAGCGCGACGTGAAGAAGG
CGACTGCGTTGATGGAGACGCACTTCGCCAGTGCGGTCGAATCGTGGAGCGAGATCCTCGGCGAGGAAGCGCCCGTGGAA
ACCAAACCGGCGCGCAAGAAGAAATAG

Upstream 100 bases:

>100_bases
GAGGCCGGCCGCCGCGTCGCTGGACGGCCAGAGCGGTGGGGGCAATCTGTATAATCTGTGGATCCCGTTTTTATGATTTT
TCTTCAGTGATATGGAACTG

Downstream 100 bases:

>100_bases
CGCGACGGCGCCAATGTGAAAGGGTGGATTCCCTGAAACGAGAATCCACCCCTTTGTTTTTGCGTGCGCGGATCAGAAGG
TGTGCAGGATGCCGAGGCGC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSPVVRESTSRSELVGKMLAFFKERGYESGERLPSERALAERFGVGRNALREALSTLSALRVIESRPNSGIYLRRMSTDS
SFETIVLLTEMGSEPSMKEVKETLEVRWALEIRGVQLACERRTEADLDSLASVIDATRAVLSNKGNIAAQDTAFHMALAR
CAHNEMLVRLLNSFYRISESRRFALFADSGRGTISAAQHESLYEAVKKRDVKKATALMETHFASAVESWSEILGEEAPVE
TKPARKKK

Sequences:

>Translated_248_residues
MSPVVRESTSRSELVGKMLAFFKERGYESGERLPSERALAERFGVGRNALREALSTLSALRVIESRPNSGIYLRRMSTDS
SFETIVLLTEMGSEPSMKEVKETLEVRWALEIRGVQLACERRTEADLDSLASVIDATRAVLSNKGNIAAQDTAFHMALAR
CAHNEMLVRLLNSFYRISESRRFALFADSGRGTISAAQHESLYEAVKKRDVKKATALMETHFASAVESWSEILGEEAPVE
TKPARKKK
>Mature_247_residues
SPVVRESTSRSELVGKMLAFFKERGYESGERLPSERALAERFGVGRNALREALSTLSALRVIESRPNSGIYLRRMSTDSS
FETIVLLTEMGSEPSMKEVKETLEVRWALEIRGVQLACERRTEADLDSLASVIDATRAVLSNKGNIAAQDTAFHMALARC
AHNEMLVRLLNSFYRISESRRFALFADSGRGTISAAQHESLYEAVKKRDVKKATALMETHFASAVESWSEILGEEAPVET
KPARKKK

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=219, Percent_Identity=30.5936073059361, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1789353, Length=189, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790780, Length=220, Percent_Identity=28.1818181818182, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI48994955, Length=221, Percent_Identity=25.7918552036199, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1789621, Length=224, Percent_Identity=26.3392857142857, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27661; Mature: 27530

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPVVRESTSRSELVGKMLAFFKERGYESGERLPSERALAERFGVGRNALREALSTLSAL
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
RVIESRPNSGIYLRRMSTDSSFETIVLLTEMGSEPSMKEVKETLEVRWALEIRGVQLACE
HHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHEECCHHHHHH
RRTEADLDSLASVIDATRAVLSNKGNIAAQDTAFHMALARCAHNEMLVRLLNSFYRISES
HHHHCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RRFALFADSGRGTISAAQHESLYEAVKKRDVKKATALMETHFASAVESWSEILGEEAPVE
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TKPARKKK
CCCCCCCC
>Mature Secondary Structure 
SPVVRESTSRSELVGKMLAFFKERGYESGERLPSERALAERFGVGRNALREALSTLSAL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
RVIESRPNSGIYLRRMSTDSSFETIVLLTEMGSEPSMKEVKETLEVRWALEIRGVQLACE
HHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHEECCHHHHHH
RRTEADLDSLASVIDATRAVLSNKGNIAAQDTAFHMALARCAHNEMLVRLLNSFYRISES
HHHHCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RRFALFADSGRGTISAAQHESLYEAVKKRDVKKATALMETHFASAVESWSEILGEEAPVE
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TKPARKKK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]