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The map label for this gene is mhpD [H]

Identifier: 91782286

GI number: 91782286

Start: 1012862

End: 1013650

Strand: Direct

Name: mhpD [H]

Synonym: Bxe_A3548

Alternate gene names: 91782286

Gene position: 1012862-1013650 (Clockwise)

Preceding gene: 91782285

Following gene: 91782287

Centisome position: 20.69

GC content: 63.75

Gene sequence:

>789_bases
ATGAGCGTAGAGAACGAAATCGCCGAGCTGCTGTGGGACGCCCAGCAGCGCAAGGAGCCCTGTGATCCTGTACGGGGGCG
CTTTGAGAACAGGAGCGAGCAGGAGCAGGTGGCACTTGCCTATTCGGTTCAGCAGATCAACATCCAGCGCCAGGTGCAGG
GTGGCAGGCGGATCGTTGGCCGGAAGATCGGCCTGACGTCTCCTGCTGTCCAGAAGCAGCTTGGGGTCGACCGTCCGGAT
TTCGGCACGCTGCTGGACGACATGGCGGTCGTTGACGGCGAACCGATTGACACGTCCCGGCTGCTGCAGCCGAAGGTTGA
AGCCGAGATCGCGCTCGTACTTGATAAGGACCTCACGTATGAGCGGCACACCGTGGCCGATCTGATCGGCGCCACCGCCT
ATGCGCTGGCCGCCATCGAGGTGGTCGGCAGCCGCATTGCAGGGTGGAATATCCGTTTCGTGGATACCGTTGCCGACAAC
GCGTCATCGGGACTGTTCGTTCTTGGCGCACAGCCGGTTGCGCTGTCGAAGCTCGACCTGGCCGGCGTATCGATGCGGAT
GACGCGCGGCGAGGAGACCGTCTCGCAAGGTGCAGGCGCGGCGTGTCTGGGCAATCCGCTGAACGCGGCGCGCTGGCTTG
CCGACACGCTCGTCCAGGTTGGGACGCCAATGCGCGCTGGCGACGTGATTCTGACTGGTGCCCTCGGCCCGATGGTTGCC
GTGCAGGCCAGCCAGACCTACGTCGCTCACATTGACGGGTTTGCCCCTGTACGGGCGATTTTCTCCTGA

Upstream 100 bases:

>100_bases
GGCAACGCAGCCCACTGGCCCCAGTATGAAGCGATCGATGAGTTCAATGACGTTGCATTGAAATTCTTCAGGACCGGTAC
GGTCGGGTAAGGAGCCGGAC

Downstream 100 bases:

>100_bases
TGAGGTAATGCACATGAGTTCCAATGACGAAAAGCACGCGGTTGCCATCATCGGCTCGGGAAACATCGGCACCGACCTGA
TGGTGAAGATCCTGCGGCAC

Product: 2-hydroxypenta-2,4-dienoate hydratase (CmtF)

Products: NA

Alternate protein names: 2-hydroxypentadienoic acid hydratase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSVENEIAELLWDAQQRKEPCDPVRGRFENRSEQEQVALAYSVQQINIQRQVQGGRRIVGRKIGLTSPAVQKQLGVDRPD
FGTLLDDMAVVDGEPIDTSRLLQPKVEAEIALVLDKDLTYERHTVADLIGATAYALAAIEVVGSRIAGWNIRFVDTVADN
ASSGLFVLGAQPVALSKLDLAGVSMRMTRGEETVSQGAGAACLGNPLNAARWLADTLVQVGTPMRAGDVILTGALGPMVA
VQASQTYVAHIDGFAPVRAIFS

Sequences:

>Translated_262_residues
MSVENEIAELLWDAQQRKEPCDPVRGRFENRSEQEQVALAYSVQQINIQRQVQGGRRIVGRKIGLTSPAVQKQLGVDRPD
FGTLLDDMAVVDGEPIDTSRLLQPKVEAEIALVLDKDLTYERHTVADLIGATAYALAAIEVVGSRIAGWNIRFVDTVADN
ASSGLFVLGAQPVALSKLDLAGVSMRMTRGEETVSQGAGAACLGNPLNAARWLADTLVQVGTPMRAGDVILTGALGPMVA
VQASQTYVAHIDGFAPVRAIFS
>Mature_261_residues
SVENEIAELLWDAQQRKEPCDPVRGRFENRSEQEQVALAYSVQQINIQRQVQGGRRIVGRKIGLTSPAVQKQLGVDRPDF
GTLLDDMAVVDGEPIDTSRLLQPKVEAEIALVLDKDLTYERHTVADLIGATAYALAAIEVVGSRIAGWNIRFVDTVADNA
SSGLFVLGAQPVALSKLDLAGVSMRMTRGEETVSQGAGAACLGNPLNAARWLADTLVQVGTPMRAGDVILTGALGPMVAV
QASQTYVAHIDGFAPVRAIFS

Specific function: Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family. MhpD subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=223, Percent_Identity=56.9506726457399, Blast_Score=255, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =4.2.1.80 [H]

Molecular weight: Translated: 28063; Mature: 27932

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVENEIAELLWDAQQRKEPCDPVRGRFENRSEQEQVALAYSVQQINIQRQVQGGRRIVG
CCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHH
RKIGLTSPAVQKQLGVDRPDFGTLLDDMAVVDGEPIDTSRLLQPKVEAEIALVLDKDLTY
HCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEEECCCCC
ERHTVADLIGATAYALAAIEVVGSRIAGWNIRFVDTVADNASSGLFVLGAQPVALSKLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEECCCCEEHHCCC
AGVSMRMTRGEETVSQGAGAACLGNPLNAARWLADTLVQVGTPMRAGDVILTGALGPMVA
CCCEEEECCCHHHHHHCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEE
VQASQTYVAHIDGFAPVRAIFS
EECCCEEEEEECCCHHHHHHCC
>Mature Secondary Structure 
SVENEIAELLWDAQQRKEPCDPVRGRFENRSEQEQVALAYSVQQINIQRQVQGGRRIVG
CCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHH
RKIGLTSPAVQKQLGVDRPDFGTLLDDMAVVDGEPIDTSRLLQPKVEAEIALVLDKDLTY
HCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEEECCCCC
ERHTVADLIGATAYALAAIEVVGSRIAGWNIRFVDTVADNASSGLFVLGAQPVALSKLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEECCCCEEHHCCC
AGVSMRMTRGEETVSQGAGAACLGNPLNAARWLADTLVQVGTPMRAGDVILTGALGPMVA
CCCEEEECCCHHHHHHCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEE
VQASQTYVAHIDGFAPVRAIFS
EECCCEEEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA