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The map label for this gene is hsaD [H]

Identifier: 91782285

GI number: 91782285

Start: 1011946

End: 1012851

Strand: Direct

Name: hsaD [H]

Synonym: Bxe_A3549

Alternate gene names: 91782285

Gene position: 1011946-1012851 (Clockwise)

Preceding gene: 91782284

Following gene: 91782286

Centisome position: 20.67

GC content: 57.28

Gene sequence:

>906_bases
GTGGCGTTGTCGTATCTGCATCTTTTCAAGGACAAATCCATGAGTCTCTGGCTTGATCTGCTCGGCGCAGAAATCCGTTA
TGTCGACACCAAGAACTTTGGCACGATCCGCATTGCCGAGGCCGGGAAAGGCAATCCCGAAACGCTCATCCTCCAGCATG
GCATCAACGGTCATCTGGAAGCTTACGCGAAGAACATCGTGCCACTGTCGAAGGATTTTCATGTCATCGCCTTCGACTAC
GTGGGTCAGGGTCTGTCCAGCAAGCCGGTGCGGGAATACAACCCGATCATGCTGGCTGAACAGCTTGGCGAACTGATGGA
CGCGCTTGGCATCGAAAAGGCGCATCTCTCGGGGGAGTCGCTTGGTGGCTGGGTTTCCGGACACTTTGCGGCCACGAACC
CCCACCGTGTCCTGCGCCTCATGCTGAACACGGCCGGCGGCATCCCCATTGTGACGGAGAAGGGCAAGCAGGACCTGGAG
ACATTTATCGGACTGAACAGGCGCAACATCGACAACACGCCGACCTACGAGTCGGTGCAGGCGCGCATGCACTGGCTGAT
GCATCCCAGCAATCGCCACCTTGTGGACGGCGAGCTGGTCGATCTGCGCCTGCGCATCTATCTGCGCCCGGAAAGCCGTG
CGGTTCTGCCGAAGCTCAACGAAATTCTCGCGCACCACGACGAGTACCTGATTCCGCTGGAGAAACTGCCGGAAGGGACG
CTTTTTCTGTGGACGCACGACAACCCGATTCACGATACCGAATCGGTGAAAGCGGCACAGGCAAAAGTTCCGGGCAGCAT
CCTGTACTTCATGAAGGGCAACGCAGCCCACTGGCCCCAGTATGAAGCGATCGATGAGTTCAATGACGTTGCATTGAAAT
TCTTCAGGACCGGTACGGTCGGGTAA

Upstream 100 bases:

>100_bases
CCTGAACGCTCCGTCACGGTAGCCAGTGGGCGCATCAGCGCGCGCCCTGCTTCAGGCTACGCTCCTCTCATTCGACGCCA
CCCCTGCGGAGTCCGCCGGG

Downstream 100 bases:

>100_bases
GGAGCCGGACATGAGCGTAGAGAACGAAATCGCCGAGCTGCTGTGGGACGCCCAGCAGCGCAAGGAGCCCTGTGATCCTG
TACGGGGGCGCTTTGAGAAC

Product: HOMODA hydrolase (CmtE)

Products: NA

Alternate protein names: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; HOPDA hydrolase; Meta-cleavage product hydrolase; MCP hydrolase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MALSYLHLFKDKSMSLWLDLLGAEIRYVDTKNFGTIRIAEAGKGNPETLILQHGINGHLEAYAKNIVPLSKDFHVIAFDY
VGQGLSSKPVREYNPIMLAEQLGELMDALGIEKAHLSGESLGGWVSGHFAATNPHRVLRLMLNTAGGIPIVTEKGKQDLE
TFIGLNRRNIDNTPTYESVQARMHWLMHPSNRHLVDGELVDLRLRIYLRPESRAVLPKLNEILAHHDEYLIPLEKLPEGT
LFLWTHDNPIHDTESVKAAQAKVPGSILYFMKGNAAHWPQYEAIDEFNDVALKFFRTGTVG

Sequences:

>Translated_301_residues
MALSYLHLFKDKSMSLWLDLLGAEIRYVDTKNFGTIRIAEAGKGNPETLILQHGINGHLEAYAKNIVPLSKDFHVIAFDY
VGQGLSSKPVREYNPIMLAEQLGELMDALGIEKAHLSGESLGGWVSGHFAATNPHRVLRLMLNTAGGIPIVTEKGKQDLE
TFIGLNRRNIDNTPTYESVQARMHWLMHPSNRHLVDGELVDLRLRIYLRPESRAVLPKLNEILAHHDEYLIPLEKLPEGT
LFLWTHDNPIHDTESVKAAQAKVPGSILYFMKGNAAHWPQYEAIDEFNDVALKFFRTGTVG
>Mature_300_residues
ALSYLHLFKDKSMSLWLDLLGAEIRYVDTKNFGTIRIAEAGKGNPETLILQHGINGHLEAYAKNIVPLSKDFHVIAFDYV
GQGLSSKPVREYNPIMLAEQLGELMDALGIEKAHLSGESLGGWVSGHFAATNPHRVLRLMLNTAGGIPIVTEKGKQDLET
FIGLNRRNIDNTPTYESVQARMHWLMHPSNRHLVDGELVDLRLRIYLRPESRAVLPKLNEILAHHDEYLIPLEKLPEGTL
FLWTHDNPIHDTESVKAAQAKVPGSILYFMKGNAAHWPQYEAIDEFNDVALKFFRTGTVG

Specific function: Catalyzes the hydrolysis of a carbon-carbon bond in 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA) to yield 9,17-dioxo-1,2,3,4,10,19- hexanorandrostan-5-oic acid (DOHNAA) and 2-hydroxy-hexa-2,4- dienoic acid (HHD). Al

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. HsaD family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=299, Percent_Identity=27.0903010033445, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.7.1.8 [H]

Molecular weight: Translated: 33835; Mature: 33704

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSYLHLFKDKSMSLWLDLLGAEIRYVDTKNFGTIRIAEAGKGNPETLILQHGINGHLE
CCCHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCCCEEEEECCCCCHHH
AYAKNIVPLSKDFHVIAFDYVGQGLSSKPVREYNPIMLAEQLGELMDALGIEKAHLSGES
HHHHHCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCC
LGGWVSGHFAATNPHRVLRLMLNTAGGIPIVTEKGKQDLETFIGLNRRNIDNTPTYESVQ
CCCEEECCEECCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHH
ARMHWLMHPSNRHLVDGELVDLRLRIYLRPESRAVLPKLNEILAHHDEYLIPLEKLPEGT
HHHHHEECCCCCEEECCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEHHHCCCCE
LFLWTHDNPIHDTESVKAAQAKVPGSILYFMKGNAAHWPQYEAIDEFNDVALKFFRTGTV
EEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
G
C
>Mature Secondary Structure 
ALSYLHLFKDKSMSLWLDLLGAEIRYVDTKNFGTIRIAEAGKGNPETLILQHGINGHLE
CCHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCCCEEEEECCCCCHHH
AYAKNIVPLSKDFHVIAFDYVGQGLSSKPVREYNPIMLAEQLGELMDALGIEKAHLSGES
HHHHHCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCC
LGGWVSGHFAATNPHRVLRLMLNTAGGIPIVTEKGKQDLETFIGLNRRNIDNTPTYESVQ
CCCEEECCEECCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHH
ARMHWLMHPSNRHLVDGELVDLRLRIYLRPESRAVLPKLNEILAHHDEYLIPLEKLPEGT
HHHHHEECCCCCEEECCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEHHHCCCCE
LFLWTHDNPIHDTESVKAAQAKVPGSILYFMKGNAAHWPQYEAIDEFNDVALKFFRTGTV
EEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA