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The map label for this gene is yisK [H]

Identifier: 91782217

GI number: 91782217

Start: 936858

End: 937772

Strand: Direct

Name: yisK [H]

Synonym: Bxe_A3618

Alternate gene names: 91782217

Gene position: 936858-937772 (Clockwise)

Preceding gene: 91782216

Following gene: 91782218

Centisome position: 19.14

GC content: 60.0

Gene sequence:

>915_bases
ATGAAGTTCACCACTTTCGCCTGCGATGGTCAACCGTGCCTCGGCGTGGCAACAGAAAACGATACTGTATTGAGTCTGAC
GCACACATGGGGTGCTGGCCCGCTAATAGACGGCGCTGCGCCGCGCGATATCGGTCAATTGATCGAGCTGGGTGACAATG
CACTCAAGGTGGTGCGAACGATCATCGAGGATCAGGCGGGAAGTGAGGTGTATCGTCACGCTGCACACACTTTGCAGTTC
CTGCCGCCGCTCGGCGTGCCTCGCAAGAATGTGTTTTGCGTCGGCCGCAATTATCGCGAGCACATTATCGAAGGCAATCT
CGCGGCGGGGCGCGATCCGCACGATTTCCCCAAGGCGCTGGAACTTTTTACAAAGCCGCCAACCGCTATCGTCGGACATC
GTGCGCCTGTGAAGTTGCACGCTCACGTCACGAAACTGCTCGACTATGAAATCGAGCTCGGTATCGTGATCGGCAAACGC
GGAATCGACATTCCGCGCGAAGCCGCGCTCGATTACGTGTTCGGCTACACGATCGTCAACGACATCACGGCACGCGATCT
GCAGAAGCGCCACGGGCAATGGTTCAAAGGCAAGGCGCTGGATACGTCCTGCCCGGTGGGGCCTTATGTGCTGCATGGCT
CGGCGGTTCGCGATCCACAGGCGTTCGAACTCGACCTCGACGTGAACGGCGAACTGCGGCAACGCGCAAGCACGGCGTCG
ATGCTGTTTGGCGTAGCGGAGATCATCGAACAGCTGTCTGCCGGCCTGACACTGGAGCCGGGGGATCTGATCGCAACAGG
CACGCCGAGCGGTGTCGGCTTCGCGATGCAGCCGCCGCGCGCGCTCGCAGCAGGCGATACGGTTCGCGCGAGGATCGGGG
GAATCGGAGAGTTGAGCAACACGATCGTCGAATAA

Upstream 100 bases:

>100_bases
GCGCGGAGCCGGAGAGATGTGCATTGCTTTGCAAGCAGCATATCCGCGCGCTGTATGACCATATCTGCCACGAGCTCCAA
TAACAACAGCTTACTCTAAG

Downstream 100 bases:

>100_bases
CGGAACGCAGATTCGCGTGCCGAGGGATTTCGAGTCGCACACTAATCCTATGCCAGTCATTTAGTAGGACAAATCAGATA
CAGGAGCAAACGTGATCTAT

Product: putative hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKFTTFACDGQPCLGVATENDTVLSLTHTWGAGPLIDGAAPRDIGQLIELGDNALKVVRTIIEDQAGSEVYRHAAHTLQF
LPPLGVPRKNVFCVGRNYREHIIEGNLAAGRDPHDFPKALELFTKPPTAIVGHRAPVKLHAHVTKLLDYEIELGIVIGKR
GIDIPREAALDYVFGYTIVNDITARDLQKRHGQWFKGKALDTSCPVGPYVLHGSAVRDPQAFELDLDVNGELRQRASTAS
MLFGVAEIIEQLSAGLTLEPGDLIATGTPSGVGFAMQPPRALAAGDTVRARIGGIGELSNTIVE

Sequences:

>Translated_304_residues
MKFTTFACDGQPCLGVATENDTVLSLTHTWGAGPLIDGAAPRDIGQLIELGDNALKVVRTIIEDQAGSEVYRHAAHTLQF
LPPLGVPRKNVFCVGRNYREHIIEGNLAAGRDPHDFPKALELFTKPPTAIVGHRAPVKLHAHVTKLLDYEIELGIVIGKR
GIDIPREAALDYVFGYTIVNDITARDLQKRHGQWFKGKALDTSCPVGPYVLHGSAVRDPQAFELDLDVNGELRQRASTAS
MLFGVAEIIEQLSAGLTLEPGDLIATGTPSGVGFAMQPPRALAAGDTVRARIGGIGELSNTIVE
>Mature_304_residues
MKFTTFACDGQPCLGVATENDTVLSLTHTWGAGPLIDGAAPRDIGQLIELGDNALKVVRTIIEDQAGSEVYRHAAHTLQF
LPPLGVPRKNVFCVGRNYREHIIEGNLAAGRDPHDFPKALELFTKPPTAIVGHRAPVKLHAHVTKLLDYEIELGIVIGKR
GIDIPREAALDYVFGYTIVNDITARDLQKRHGQWFKGKALDTSCPVGPYVLHGSAVRDPQAFELDLDVNGELRQRASTAS
MLFGVAEIIEQLSAGLTLEPGDLIATGTPSGVGFAMQPPRALAAGDTVRARIGGIGELSNTIVE

Specific function: Unknown

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI40786394, Length=270, Percent_Identity=35.9259259259259, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI156231349, Length=270, Percent_Identity=35.1851851851852, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI215422413, Length=216, Percent_Identity=41.2037037037037, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI66348062, Length=189, Percent_Identity=44.4444444444444, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI13654274, Length=189, Percent_Identity=44.4444444444444, Blast_Score=146, Evalue=2e-35,
Organism=Escherichia coli, GI1787428, Length=194, Percent_Identity=37.1134020618557, Blast_Score=118, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17557057, Length=186, Percent_Identity=38.1720430107527, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324161, Length=241, Percent_Identity=29.045643153527, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28572127, Length=226, Percent_Identity=42.4778761061947, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24663695, Length=227, Percent_Identity=33.920704845815, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28571789, Length=215, Percent_Identity=33.4883720930233, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: NA

Molecular weight: Translated: 32709; Mature: 32709

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTTFACDGQPCLGVATENDTVLSLTHTWGAGPLIDGAAPRDIGQLIELGDNALKVVRT
CCEEEEEECCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHH
IIEDQAGSEVYRHAAHTLQFLPPLGVPRKNVFCVGRNYREHIIEGNLAAGRDPHDFPKAL
HHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCHHHHHHH
ELFTKPPTAIVGHRAPVKLHAHVTKLLDYEIELGIVIGKRGIDIPREAALDYVFGYTIVN
HHHCCCCCCEECCCCCEEHHHHHHHHHCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
DITARDLQKRHGQWFKGKALDTSCPVGPYVLHGSAVRDPQAFELDLDVNGELRQRASTAS
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCHHHHHHHHHH
MLFGVAEIIEQLSAGLTLEPGDLIATGTPSGVGFAMQPPRALAAGDTVRARIGGIGELSN
HHHHHHHHHHHHHCCCEECCCCEEEECCCCCCCCCCCCCCHHHCCCHHHHHHCCCHHHHH
TIVE
HHCC
>Mature Secondary Structure
MKFTTFACDGQPCLGVATENDTVLSLTHTWGAGPLIDGAAPRDIGQLIELGDNALKVVRT
CCEEEEEECCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHH
IIEDQAGSEVYRHAAHTLQFLPPLGVPRKNVFCVGRNYREHIIEGNLAAGRDPHDFPKAL
HHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCHHHHHHH
ELFTKPPTAIVGHRAPVKLHAHVTKLLDYEIELGIVIGKRGIDIPREAALDYVFGYTIVN
HHHCCCCCCEECCCCCEEHHHHHHHHHCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
DITARDLQKRHGQWFKGKALDTSCPVGPYVLHGSAVRDPQAFELDLDVNGELRQRASTAS
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCHHHHHHHHHH
MLFGVAEIIEQLSAGLTLEPGDLIATGTPSGVGFAMQPPRALAAGDTVRARIGGIGELSN
HHHHHHHHHHHHHCCCEECCCCEEEECCCCCCCCCCCCCCHHHCCCHHHHHHCCCHHHHH
TIVE
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]