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The map label for this gene is waaA [H]

Identifier: 91782137

GI number: 91782137

Start: 850366

End: 851685

Strand: Direct

Name: waaA [H]

Synonym: Bxe_A3698

Alternate gene names: 91782137

Gene position: 850366-851685 (Clockwise)

Preceding gene: 91782136

Following gene: 91782159

Centisome position: 17.37

GC content: 67.88

Gene sequence:

>1320_bases
ATGCTGAGGACCGTCTATCGCGCGCTCTGGTGGATCATCGCGCCGCTGGCCGTGCTGCGCCTCATGATCCGCTCGCGCAA
GGAGCGCGGCTATCGCGAGCATATCGGCGAGCGTTTCGGTTACTCGCGCGGCCGGCTGCCTGAAGACAATGCGCCGCTGA
TCTGGGTGCACGCGGTGTCGGTGGGCGAGACGCGCGCCGCGCAACCGCTGATCGAAGCGTTGATGAAGGCGCGTCCAGAC
GCACGCATTCTGTTGACCCACATGACGCCGAGCGGGCGCGCCACCGGCGAGCAGATTTTCGGCGACCGCGTTTTGCGCAG
CTATTTGCCGTACGACATGCCGCATGCCGTGCGGCGGTTTTTGCGGACATGGCGGCCGTCGCTCGGTCTGGTGATGGAAA
CCGAAGTGTGGCCGACGCTGATCGACGAATGCCGTCGCGCGGACGTGCCGCTGGTGCTGACCAATGCGCGGATGTCGGCG
CGCTCGTATAAGCGCGCGGCGAAGTTCGGCAGTGCGACCAAAGACGTGTTCGGCGGCTTTGCGCGTGTGCTGGCGCAGAG
TCCGTCGGATGCCGAGCGGCTGACCGCGCTCGGCGCGCGCAACGCGGCGGTGCTCGGCAATCTGAAATTCGACATGACCA
CGCCGCCGGAACTGGCCGCGCGCGGGCATGCGTGGCGCGCGGCGATCGGCGCGCGTCCGGTGTGGGTTGCGGCGAGCACG
CGCGAGGGCGAAGAGGAACTGGTGCTGCAGGCGTTCGCGGCGCTCGGCATCGACAACGCGCTGCTGATTCTGGTGCCGCG
TCATCCGCAACGTTTCAACGAAGTCGCGGGGCTGGTCGAGAAAGCCGGGCTGCGGCTCGAGCGGCGTTCGGCCTGGGCGC
CGGATGCGAAGGTTGCCTCGGCGGCGGTGTCCGCGAGCGGCGGTTTGCCGGCTTTGCCCGCACACGTGAACGTGCTGCTG
GGCGATTCGATGGGCGAGTTGGGCGCTTACTATGCGGCTTCGGATCTGGCGTTCATCGGCGGAAGTTTGCTGCCGTTGGG
CGGGCAGAATCTGATCGAAGCGTGCGCGGTCGGTGTGCCGGTGCTGATCGGGCCGCACGTGTTCAACTTCACGCAGGCCA
CTGCGGATGCCGTGGCGGCGGGCGCCGCGGTTCAGGTGCAGGATCCGGCCGATCTGGCGCGGGCATTACGCGAATTGTTC
GGCGACAAGGCCAGAAGACTGGCCATGAGCGGCGCGGCTTCGGCGTTCGCCGCGCGTCACCGCGGCGCGACCGCGCGGAC
CGTGGATGTGTTGATGGCGTTGTTGCCGGAGGTGGAGTGA

Upstream 100 bases:

>100_bases
CTCGAACGCCTCAAGGGCAGGGCGCTGGTTTGAGCGCCGCGGTCCGTTTTGCGGTTTCGTTCGGCGCGTCGGCTTCTCCG
TTCAGGCACGCGGAGTGCCG

Downstream 100 bases:

>100_bases
GGGCGGGGTGCAAAAGGCGTCATTGCACCCGGGCCTCAAACCTTCAACAACCCCTTCTTCTCGATAAACTTCACCACCAC
GTCCAGCCCGTCGAGCGCCT

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MLRTVYRALWWIIAPLAVLRLMIRSRKERGYREHIGERFGYSRGRLPEDNAPLIWVHAVSVGETRAAQPLIEALMKARPD
ARILLTHMTPSGRATGEQIFGDRVLRSYLPYDMPHAVRRFLRTWRPSLGLVMETEVWPTLIDECRRADVPLVLTNARMSA
RSYKRAAKFGSATKDVFGGFARVLAQSPSDAERLTALGARNAAVLGNLKFDMTTPPELAARGHAWRAAIGARPVWVAAST
REGEEELVLQAFAALGIDNALLILVPRHPQRFNEVAGLVEKAGLRLERRSAWAPDAKVASAAVSASGGLPALPAHVNVLL
GDSMGELGAYYAASDLAFIGGSLLPLGGQNLIEACAVGVPVLIGPHVFNFTQATADAVAAGAAVQVQDPADLARALRELF
GDKARRLAMSGAASAFAARHRGATARTVDVLMALLPEVE

Sequences:

>Translated_439_residues
MLRTVYRALWWIIAPLAVLRLMIRSRKERGYREHIGERFGYSRGRLPEDNAPLIWVHAVSVGETRAAQPLIEALMKARPD
ARILLTHMTPSGRATGEQIFGDRVLRSYLPYDMPHAVRRFLRTWRPSLGLVMETEVWPTLIDECRRADVPLVLTNARMSA
RSYKRAAKFGSATKDVFGGFARVLAQSPSDAERLTALGARNAAVLGNLKFDMTTPPELAARGHAWRAAIGARPVWVAAST
REGEEELVLQAFAALGIDNALLILVPRHPQRFNEVAGLVEKAGLRLERRSAWAPDAKVASAAVSASGGLPALPAHVNVLL
GDSMGELGAYYAASDLAFIGGSLLPLGGQNLIEACAVGVPVLIGPHVFNFTQATADAVAAGAAVQVQDPADLARALRELF
GDKARRLAMSGAASAFAARHRGATARTVDVLMALLPEVE
>Mature_439_residues
MLRTVYRALWWIIAPLAVLRLMIRSRKERGYREHIGERFGYSRGRLPEDNAPLIWVHAVSVGETRAAQPLIEALMKARPD
ARILLTHMTPSGRATGEQIFGDRVLRSYLPYDMPHAVRRFLRTWRPSLGLVMETEVWPTLIDECRRADVPLVLTNARMSA
RSYKRAAKFGSATKDVFGGFARVLAQSPSDAERLTALGARNAAVLGNLKFDMTTPPELAARGHAWRAAIGARPVWVAAST
REGEEELVLQAFAALGIDNALLILVPRHPQRFNEVAGLVEKAGLRLERRSAWAPDAKVASAAVSASGGLPALPAHVNVLL
GDSMGELGAYYAASDLAFIGGSLLPLGGQNLIEACAVGVPVLIGPHVFNFTQATADAVAAGAAVQVQDPADLARALRELF
GDKARRLAMSGAASAFAARHRGATARTVDVLMALLPEVE

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=441, Percent_Identity=41.7233560090703, Blast_Score=302, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 47419; Mature: 47419

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTVYRALWWIIAPLAVLRLMIRSRKERGYREHIGERFGYSRGRLPEDNAPLIWVHAVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEE
VGETRAAQPLIEALMKARPDARILLTHMTPSGRATGEQIFGDRVLRSYLPYDMPHAVRRF
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
LRTWRPSLGLVMETEVWPTLIDECRRADVPLVLTNARMSARSYKRAAKFGSATKDVFGGF
HHHHCCCCCEEEECHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHH
ARVLAQSPSDAERLTALGARNAAVLGNLKFDMTTPPELAARGHAWRAAIGARPVWVAAST
HHHHHCCCCHHHHHHHHCCCCCEEEECEEECCCCCHHHHHCCCHHHHHHCCCCEEEEECC
REGEEELVLQAFAALGIDNALLILVPRHPQRFNEVAGLVEKAGLRLERRSAWAPDAKVAS
CCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHH
AAVSASGGLPALPAHVNVLLGDSMGELGAYYAASDLAFIGGSLLPLGGQNLIEACAVGVP
HHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
VLIGPHVFNFTQATADAVAAGAAVQVQDPADLARALRELFGDKARRLAMSGAASAFAARH
CEECCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGATARTVDVLMALLPEVE
CCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLRTVYRALWWIIAPLAVLRLMIRSRKERGYREHIGERFGYSRGRLPEDNAPLIWVHAVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEE
VGETRAAQPLIEALMKARPDARILLTHMTPSGRATGEQIFGDRVLRSYLPYDMPHAVRRF
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
LRTWRPSLGLVMETEVWPTLIDECRRADVPLVLTNARMSARSYKRAAKFGSATKDVFGGF
HHHHCCCCCEEEECHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHH
ARVLAQSPSDAERLTALGARNAAVLGNLKFDMTTPPELAARGHAWRAAIGARPVWVAAST
HHHHHCCCCHHHHHHHHCCCCCEEEECEEECCCCCHHHHHCCCHHHHHHCCCCEEEEECC
REGEEELVLQAFAALGIDNALLILVPRHPQRFNEVAGLVEKAGLRLERRSAWAPDAKVAS
CCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHH
AAVSASGGLPALPAHVNVLLGDSMGELGAYYAASDLAFIGGSLLPLGGQNLIEACAVGVP
HHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
VLIGPHVFNFTQATADAVAAGAAVQVQDPADLARALRELFGDKARRLAMSGAASAFAARH
CEECCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGATARTVDVLMALLPEVE
CCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]