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The map label for this gene is rfbB [H]

Identifier: 91782108

GI number: 91782108

Start: 819997

End: 821058

Strand: Direct

Name: rfbB [H]

Synonym: Bxe_A3727

Alternate gene names: 91782108

Gene position: 819997-821058 (Clockwise)

Preceding gene: 91782107

Following gene: 91782109

Centisome position: 16.75

GC content: 62.15

Gene sequence:

>1062_bases
ATGATCCTGGTGACAGGCGGCGCCGGTTTTATCGGCGCCAATTTCGTGCTCGACTGGCTGCGGCTCCATGACGAACCGCT
AGTGAATGTCGACAAGCTGACTTATGCGGGCAATCTCGGTACGCTGAAATCTCGGCAGCACGATCCATTGCATATTTTCG
TGCGCGCGGATATCTGCGATCGCAGCGCACTCGACGCGTTGTTTGCCGAGCACAAGCCGCGTGCCGTGCTGCACTTTGCC
GCGGAAAGCCACGTGGACCGTTCGATTCATGGCCCGGCTGATTTCGTGCAAACGAACGTGGTCGGCACCTTCACGCTGCT
CGAGGCAACCCGTCAGTACTGGAACGCGCTCGGCGAAGCCGGCAAGGCCGCATTCCGCTTCCTGCACGTTTCGACCGACG
AAGTATTCGGCTCGCTGTCCGCTACCGATCCGCAGTTCTCCGAAACCACGCCGTATGCGCCGAACAGTCCTTACTCGGCC
ACCAAGGCCGGTTCCGACCATCTGGTGCGCGCCTACCATCATACGTATGGGCTGCCGGTGCTGACCACGAACTGCTCGAA
CAACTACGGTCCGTACCAGTTCCCCGAAAAGCTGATTCCGCTGATGATCGCCAGTGCGCTCGCCGGCAAGGCGCTGCCGG
TCTACGGCGACGGCCAGAACGTGCGCGACTGGCTGTACGTGGGCGACCACTGCAGCGCGATCCGCGAGGTGCTCGCGCGC
GGCACACCCGGCGAGACGTACAACGTGGGCGGCTGGAACGAGAAGAAGAACCTCGAAGTGGTCCACACGCTGTGCGACCT
GCTCGACCGGTTGCGTCCGAAGGCGGGCGCGTCGTACCACGACCAGATCACGTATGTGAAAGATCGTCCGGGCCACGACC
GCCGTTATGCGATCGACGCGCGCAAGCTCGAACGTGAACTTGGGTGGAAGCCCGCGGAAACGTTCGAAACGGGTCTCGCG
AAGACGGTCCGGTGGTATCTGGACAATCAGGCGTGGTCGAACGAAGTCGCCTCGGGCGAGTACCGCAAGTGGGTTGAGAC
CAACTACGCGCAGCGCGCGTAA

Upstream 100 bases:

>100_bases
TGCTTAGCGACATAACGTGGATGTGTCGTCCACGTCAGGTCAGCCGGACTCCAATAGACCTTCGGCGTACTTGCGCGAAG
GTGGCTTCCAGAGGGCTTCC

Downstream 100 bases:

>100_bases
GGGCAAGGCAATGGCGCGTAAAGGCATTATTCTCGCGGGCGGCTCGGGCACGCGCCTGTATCCGATCACGCACGTCGTCT
CCAAGCAGTTGCTGCCGGTG

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MILVTGGAGFIGANFVLDWLRLHDEPLVNVDKLTYAGNLGTLKSRQHDPLHIFVRADICDRSALDALFAEHKPRAVLHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEATRQYWNALGEAGKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIASALAGKALPVYGDGQNVRDWLYVGDHCSAIREVLAR
GTPGETYNVGGWNEKKNLEVVHTLCDLLDRLRPKAGASYHDQITYVKDRPGHDRRYAIDARKLERELGWKPAETFETGLA
KTVRWYLDNQAWSNEVASGEYRKWVETNYAQRA

Sequences:

>Translated_353_residues
MILVTGGAGFIGANFVLDWLRLHDEPLVNVDKLTYAGNLGTLKSRQHDPLHIFVRADICDRSALDALFAEHKPRAVLHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEATRQYWNALGEAGKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIASALAGKALPVYGDGQNVRDWLYVGDHCSAIREVLAR
GTPGETYNVGGWNEKKNLEVVHTLCDLLDRLRPKAGASYHDQITYVKDRPGHDRRYAIDARKLERELGWKPAETFETGLA
KTVRWYLDNQAWSNEVASGEYRKWVETNYAQRA
>Mature_353_residues
MILVTGGAGFIGANFVLDWLRLHDEPLVNVDKLTYAGNLGTLKSRQHDPLHIFVRADICDRSALDALFAEHKPRAVLHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEATRQYWNALGEAGKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIASALAGKALPVYGDGQNVRDWLYVGDHCSAIREVLAR
GTPGETYNVGGWNEKKNLEVVHTLCDLLDRLRPKAGASYHDQITYVKDRPGHDRRYAIDARKLERELGWKPAETFETGLA
KTVRWYLDNQAWSNEVASGEYRKWVETNYAQRA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=35.0148367952522, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI42516563, Length=331, Percent_Identity=23.8670694864048, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI56237023, Length=360, Percent_Identity=25.8333333333333, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI56118217, Length=360, Percent_Identity=25.8333333333333, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI189083684, Length=360, Percent_Identity=25.8333333333333, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4504031, Length=277, Percent_Identity=25.9927797833935, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=60.4456824512535, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=62.2093023255814, Blast_Score=443, Evalue=1e-126,
Organism=Escherichia coli, GI1786974, Length=348, Percent_Identity=24.4252873563218, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788366, Length=292, Percent_Identity=27.7397260273973, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=333, Percent_Identity=36.9369369369369, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=33.8368580060423, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71982035, Length=362, Percent_Identity=25.9668508287293, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=364, Percent_Identity=25.8241758241758, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=23.2628398791541, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=278, Percent_Identity=25.8992805755396, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI133901786, Length=191, Percent_Identity=29.3193717277487, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=191, Percent_Identity=29.3193717277487, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=191, Percent_Identity=29.3193717277487, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=191, Percent_Identity=29.3193717277487, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=191, Percent_Identity=29.3193717277487, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=363, Percent_Identity=25.6198347107438, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19923002, Length=363, Percent_Identity=28.099173553719, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356223, Length=339, Percent_Identity=25.0737463126844, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24158427, Length=277, Percent_Identity=26.3537906137184, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39448; Mature: 39448

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGGAGFIGANFVLDWLRLHDEPLVNVDKLTYAGNLGTLKSRQHDPLHIFVRADICD
CEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCCCCCCCCEEEEEEECCCC
RSALDALFAEHKPRAVLHFAAESHVDRSIHGPADFVQTNVVGTFTLLEATRQYWNALGEA
HHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSATKAGSDHLVRAYHHTYGLPV
HHHHEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLMIASALAGKALPVYGDGQNVRDWLYVGDHCSAIREVLAR
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECHHHHHHHHHHHC
GTPGETYNVGGWNEKKNLEVVHTLCDLLDRLRPKAGASYHDQITYVKDRPGHDRRYAIDA
CCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEEHHH
RKLERELGWKPAETFETGLAKTVRWYLDNQAWSNEVASGEYRKWVETNYAQRA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MILVTGGAGFIGANFVLDWLRLHDEPLVNVDKLTYAGNLGTLKSRQHDPLHIFVRADICD
CEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCCCCCCCCEEEEEEECCCC
RSALDALFAEHKPRAVLHFAAESHVDRSIHGPADFVQTNVVGTFTLLEATRQYWNALGEA
HHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSATKAGSDHLVRAYHHTYGLPV
HHHHEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLMIASALAGKALPVYGDGQNVRDWLYVGDHCSAIREVLAR
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECHHHHHHHHHHHC
GTPGETYNVGGWNEKKNLEVVHTLCDLLDRLRPKAGASYHDQITYVKDRPGHDRRYAIDA
CCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEEHHH
RKLERELGWKPAETFETGLAKTVRWYLDNQAWSNEVASGEYRKWVETNYAQRA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]