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The map label for this gene is ldhA [H]

Identifier: 91781949

GI number: 91781949

Start: 632795

End: 633793

Strand: Reverse

Name: ldhA [H]

Synonym: Bxe_A3894

Alternate gene names: 91781949

Gene position: 633793-632795 (Counterclockwise)

Preceding gene: 91781953

Following gene: 91781947

Centisome position: 12.95

GC content: 65.87

Gene sequence:

>999_bases
ATGCGCATCATTCTTTTCAGCAGCCGCCAGTACGACAGCGACACCTTTACCGAAGCCAATGCATCGCACCGCTACGAACT
GCATTTCCAGGAATCGCACCTGGATAGCGAGACTGCCGTTCTCGCCCAGGGCTATGAAGTGGTGTGCCCGTTCGTCAACG
ACCACGTCGATGCGGCGGTGCTCGAACGGCTTCACGCCGGCGGCACGCGGATGATCGCATTGCGCTCGGCGGGCTTCAAT
CATGTCGATCTGGACGCGGCCGCGCGGCTCGGCATCACGGTCGCGCGGGTGCCCGCGTATTCGCCGCATGCGGTGGCCGA
GCATGCGGTTGGTCTGATTCTGGCGCTGAACCGGCGCCTGCCGCGCGCCGCCGCACGCACGCGCGAAGGCGACTTTTCGC
TGCACGGGCTGCTCGGCTTCGATCTGCATGGCAAGACCGTGGGCGTGGTCGGCACCGGCATGATCGGTCGCGTGTTCGGT
CGCATCATGGCGGGCTTCGGCATGCAGGTGCTCGCGCACGATCCCGGCACGCCCGCCGACGACCTGCTCGCGCTAGGCGC
CCGCTACGTGCCGCTCGACACACTGCTCGCCGGGTCCGACATAGTCAGCCTGCATTGTCCGCTGGTGGCCGGCACCTACC
ATCTGATCGACGGTCCCGCGCTCGCCAGAATGAAGCGCGGCGCGATGCTGATCAATACGGGCCGCGGCGGCCTCGTCGAA
AGCAATGCGCTGATCGGCGCATTGAAGGACGGTCAGCTCGGCCACCTCGGGCTCGACGTGTACGAGGAAGAAGGCGGCCT
GTTCTTCGAGGATCACTCGAACCTGCCCTTGCAGGACGACGTACTGGCGCGTCTGCTGATGTTCCCGAACGTGATCGTCA
CCGCGCATCAGGCGTTCTTCACCCGTGAGGCGATGAGCGAGATCGCCCAGACCACGCTCGCGAACGTCGCGGCATGGCGC
AACGGCACACCGCGCAACGTGGTGCGCGCGCCCGTTTAG

Upstream 100 bases:

>100_bases
AGTTCGGTCAGACTGTCAATATTATTTTCAAATCCCGGCCGATTCGTCTTCGCGAAAAATGATCTTTTAGCGCCCCGTAT
GTATTACCTCCGGAGCTCAC

Downstream 100 bases:

>100_bases
ACGCCGCCGCCGCTGTTCAGCGAAGTCGCGCGGACCACCGTGCGCGCGTCGTCCGCCGCGCCGCAGAGTTCGCGCAGCAA
CAGCGCCTCGCGTTCGTGCA

Product: putative D-lactate dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MRIILFSSRQYDSDTFTEANASHRYELHFQESHLDSETAVLAQGYEVVCPFVNDHVDAAVLERLHAGGTRMIALRSAGFN
HVDLDAAARLGITVARVPAYSPHAVAEHAVGLILALNRRLPRAAARTREGDFSLHGLLGFDLHGKTVGVVGTGMIGRVFG
RIMAGFGMQVLAHDPGTPADDLLALGARYVPLDTLLAGSDIVSLHCPLVAGTYHLIDGPALARMKRGAMLINTGRGGLVE
SNALIGALKDGQLGHLGLDVYEEEGGLFFEDHSNLPLQDDVLARLLMFPNVIVTAHQAFFTREAMSEIAQTTLANVAAWR
NGTPRNVVRAPV

Sequences:

>Translated_332_residues
MRIILFSSRQYDSDTFTEANASHRYELHFQESHLDSETAVLAQGYEVVCPFVNDHVDAAVLERLHAGGTRMIALRSAGFN
HVDLDAAARLGITVARVPAYSPHAVAEHAVGLILALNRRLPRAAARTREGDFSLHGLLGFDLHGKTVGVVGTGMIGRVFG
RIMAGFGMQVLAHDPGTPADDLLALGARYVPLDTLLAGSDIVSLHCPLVAGTYHLIDGPALARMKRGAMLINTGRGGLVE
SNALIGALKDGQLGHLGLDVYEEEGGLFFEDHSNLPLQDDVLARLLMFPNVIVTAHQAFFTREAMSEIAQTTLANVAAWR
NGTPRNVVRAPV
>Mature_332_residues
MRIILFSSRQYDSDTFTEANASHRYELHFQESHLDSETAVLAQGYEVVCPFVNDHVDAAVLERLHAGGTRMIALRSAGFN
HVDLDAAARLGITVARVPAYSPHAVAEHAVGLILALNRRLPRAAARTREGDFSLHGLLGFDLHGKTVGVVGTGMIGRVFG
RIMAGFGMQVLAHDPGTPADDLLALGARYVPLDTLLAGSDIVSLHCPLVAGTYHLIDGPALARMKRGAMLINTGRGGLVE
SNALIGALKDGQLGHLGLDVYEEEGGLFFEDHSNLPLQDDVLARLLMFPNVIVTAHQAFFTREAMSEIAQTTLANVAAWR
NGTPRNVVRAPV

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=233, Percent_Identity=33.0472103004292, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI61743967, Length=258, Percent_Identity=35.2713178294574, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI4557497, Length=258, Percent_Identity=35.2713178294574, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI145580578, Length=264, Percent_Identity=33.7121212121212, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4557499, Length=264, Percent_Identity=33.7121212121212, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI145580575, Length=264, Percent_Identity=33.7121212121212, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI6912396, Length=294, Percent_Identity=29.5918367346939, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1787645, Length=322, Percent_Identity=51.8633540372671, Blast_Score=337, Evalue=9e-94,
Organism=Escherichia coli, GI87082289, Length=281, Percent_Identity=33.4519572953737, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789279, Length=284, Percent_Identity=32.3943661971831, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788660, Length=216, Percent_Identity=32.4074074074074, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=265, Percent_Identity=29.0566037735849, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25147481, Length=253, Percent_Identity=28.8537549407115, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320925, Length=311, Percent_Identity=31.5112540192926, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=246, Percent_Identity=35.3658536585366, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324055, Length=245, Percent_Identity=30.2040816326531, Blast_Score=108, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6324964, Length=212, Percent_Identity=30.188679245283, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=30.7142857142857, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24646446, Length=260, Percent_Identity=36.1538461538462, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646448, Length=260, Percent_Identity=36.1538461538462, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646452, Length=260, Percent_Identity=36.1538461538462, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646450, Length=260, Percent_Identity=36.1538461538462, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI62472511, Length=260, Percent_Identity=36.1538461538462, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI19921140, Length=260, Percent_Identity=27.6923076923077, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI28574286, Length=270, Percent_Identity=31.1111111111111, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45552429, Length=270, Percent_Identity=30, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585514, Length=270, Percent_Identity=30, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28574282, Length=270, Percent_Identity=30, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28574284, Length=270, Percent_Identity=30, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI45551003, Length=270, Percent_Identity=30, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI28571528, Length=289, Percent_Identity=30.1038062283737, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585516, Length=266, Percent_Identity=24.812030075188, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 35708; Mature: 35708

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIILFSSRQYDSDTFTEANASHRYELHFQESHLDSETAVLAQGYEVVCPFVNDHVDAAV
CEEEEEECCCCCCCCCCCCCCCCEEEEEEEHHCCCCHHHHHHCCCEEEECCCCCCHHHHH
LERLHAGGTRMIALRSAGFNHVDLDAAARLGITVARVPAYSPHAVAEHAVGLILALNRRL
HHHHHCCCEEEEEEECCCCCEEEHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCC
PRAAARTREGDFSLHGLLGFDLHGKTVGVVGTGMIGRVFGRIMAGFGMQVLAHDPGTPAD
CHHHHHCCCCCEEEEEEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCHH
DLLALGARYVPLDTLLAGSDIVSLHCPLVAGTYHLIDGPALARMKRGAMLINTGRGGLVE
HHHHHCCCCCCHHHHHCCCCEEEEECCHHCCEEEEECCHHHHHHHCCEEEEECCCCCEEE
SNALIGALKDGQLGHLGLDVYEEEGGLFFEDHSNLPLQDDVLARLLMFPNVIVTAHQAFF
CCCEEEEECCCCCCEECCEEEECCCCEEEECCCCCCCCHHHHHHHHHCCCCEEHHHHHHH
TREAMSEIAQTTLANVAAWRNGTPRNVVRAPV
HHHHHHHHHHHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure
MRIILFSSRQYDSDTFTEANASHRYELHFQESHLDSETAVLAQGYEVVCPFVNDHVDAAV
CEEEEEECCCCCCCCCCCCCCCCEEEEEEEHHCCCCHHHHHHCCCEEEECCCCCCHHHHH
LERLHAGGTRMIALRSAGFNHVDLDAAARLGITVARVPAYSPHAVAEHAVGLILALNRRL
HHHHHCCCEEEEEEECCCCCEEEHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCC
PRAAARTREGDFSLHGLLGFDLHGKTVGVVGTGMIGRVFGRIMAGFGMQVLAHDPGTPAD
CHHHHHCCCCCEEEEEEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCHH
DLLALGARYVPLDTLLAGSDIVSLHCPLVAGTYHLIDGPALARMKRGAMLINTGRGGLVE
HHHHHCCCCCCHHHHHCCCCEEEEECCHHCCEEEEECCHHHHHHHCCEEEEECCCCCEEE
SNALIGALKDGQLGHLGLDVYEEEGGLFFEDHSNLPLQDDVLARLLMFPNVIVTAHQAFF
CCCEEEEECCCCCCEECCEEEECCCCEEEECCCCCCCCHHHHHHHHHCCCCEEHHHHHHH
TREAMSEIAQTTLANVAAWRNGTPRNVVRAPV
HHHHHHHHHHHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]