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The map label for this gene is glcF [H]

Identifier: 91781865

GI number: 91781865

Start: 539246

End: 540472

Strand: Reverse

Name: glcF [H]

Synonym: Bxe_A3980

Alternate gene names: 91781865

Gene position: 540472-539246 (Counterclockwise)

Preceding gene: 91781866

Following gene: 91781864

Centisome position: 11.04

GC content: 65.2

Gene sequence:

>1227_bases
ATGCAAACCAACCTCGCGGACTTCATGCGCAACACGCCCGATGGCGACGAAGCCGACGGCATTCTGCGCAACTGCGTGCA
TTGCGGTTTCTGCACGGCTACCTGTCCGACCTATCAGCTGCTCGGCGACGAACTCGACGGCCCGCGCGGACGGATCTATC
TGATCAAGCAGATGGTGGAAGGCGCACCGGTCACGCGCAGCACCCAGGTCCACCTCGACCGCTGCCTGACCTGCCGCAAC
TGCGAAACGACCTGCCCCTCCGGCGTGCAATATGGCCGGCTGGTGGAGATCGGCCGCAAGATCACCGAAGAAAAAGTCAC
GCGTCCGCTCGGCCAGAGGCTGATGCGCCGGATGCTCGCGAGCTTCGTGCCGAGCAGCGCGCTGTTCACGCCGACCATGC
GTATCGGTCAGCATTTTCGGCCGTTGCTGCCGAGGAAGCTGCGCGACAAGGTGCCCGCGCGGCAGCGTCCGCTGGAATGG
CCGACCGCGAAGCATCCGCGCAAGATGCTGCTGCTCGCGGGCTGCGTGCAACCGTCGATGATGCCGAACGTCAACGTCGC
GACCGCGCGCGTGCTCGACGCGCTCGGCATCGAAACGCTGATCGCGCCGGATGCCGGCTGCTGCGGCGCGATCCGTCTGC
ATCTGGGCTACAACGACGACGCCCTCGACGACCTGCGCGCCAACATCGACGCATGGTGGCCGTACGTCGAACAGGGCGTG
GAAGCGATCGTGATGAACGCGTCCGGCTGCGGCGCGACCGTCAAGGAATACGCGCACCTGTTGCGCGACGATCCGGCGTA
TGCGGAAAAAGCGCGCCGCGTGGTGGAACTGACGCGCGACATCGCGGAGATCCTGCCGGAGTTCGAAGAATCGCTGGTTT
CCGTCACGCGGCGCCGCTCGGTGCATACGGTCGCGTTTCATCCGCCCTGCACGCTGCAGCATGGCCAGCAGATACGCGGC
AAGGTCGAGCACCTGCTCGGCGCGCTGGGTGTCGAAGTGCGACTGCCCACCGACAATCATCTATGCTGCGGCTCGGCCGG
CACCTACTCGCTGACGCAGCCCAAGCTTTCGTATGCGTTGCGGGATCAGAAGCTCGAACGGCTGCAGGCGCAGGAGCCGC
AGGTGATCGTGTCGGCGAACGTCGGCTGCATTGCGCATCTGCAAAGCGGCACGTCCACGCCGGTTGCGCACTGGATCGAA
CTGGTCGAGCACATGCTGTCCGTATAA

Upstream 100 bases:

>100_bases
CCGCGCCGCTCATGAAAATCCATCGCGGCCTGAAAGCCGCTTTCGACCCGGCCCGCATCTTCAATCGCGGCCGTCTCTAC
CCCGACTTCTGAGCGACGCG

Downstream 100 bases:

>100_bases
TCGGGTAATCGACTTTCGCTGGACTTCCGGATCCGTCATGCCCGATCTGATTCACAACCTCGAAGCGGTGCGGCAGCGCA
TCGCCACGGCCGCGCACGTG

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQTNLADFMRNTPDGDEADGILRNCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVEGAPVTRSTQVHLDRCLTCRN
CETTCPSGVQYGRLVEIGRKITEEKVTRPLGQRLMRRMLASFVPSSALFTPTMRIGQHFRPLLPRKLRDKVPARQRPLEW
PTAKHPRKMLLLAGCVQPSMMPNVNVATARVLDALGIETLIAPDAGCCGAIRLHLGYNDDALDDLRANIDAWWPYVEQGV
EAIVMNASGCGATVKEYAHLLRDDPAYAEKARRVVELTRDIAEILPEFEESLVSVTRRRSVHTVAFHPPCTLQHGQQIRG
KVEHLLGALGVEVRLPTDNHLCCGSAGTYSLTQPKLSYALRDQKLERLQAQEPQVIVSANVGCIAHLQSGTSTPVAHWIE
LVEHMLSV

Sequences:

>Translated_408_residues
MQTNLADFMRNTPDGDEADGILRNCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVEGAPVTRSTQVHLDRCLTCRN
CETTCPSGVQYGRLVEIGRKITEEKVTRPLGQRLMRRMLASFVPSSALFTPTMRIGQHFRPLLPRKLRDKVPARQRPLEW
PTAKHPRKMLLLAGCVQPSMMPNVNVATARVLDALGIETLIAPDAGCCGAIRLHLGYNDDALDDLRANIDAWWPYVEQGV
EAIVMNASGCGATVKEYAHLLRDDPAYAEKARRVVELTRDIAEILPEFEESLVSVTRRRSVHTVAFHPPCTLQHGQQIRG
KVEHLLGALGVEVRLPTDNHLCCGSAGTYSLTQPKLSYALRDQKLERLQAQEPQVIVSANVGCIAHLQSGTSTPVAHWIE
LVEHMLSV
>Mature_408_residues
MQTNLADFMRNTPDGDEADGILRNCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVEGAPVTRSTQVHLDRCLTCRN
CETTCPSGVQYGRLVEIGRKITEEKVTRPLGQRLMRRMLASFVPSSALFTPTMRIGQHFRPLLPRKLRDKVPARQRPLEW
PTAKHPRKMLLLAGCVQPSMMPNVNVATARVLDALGIETLIAPDAGCCGAIRLHLGYNDDALDDLRANIDAWWPYVEQGV
EAIVMNASGCGATVKEYAHLLRDDPAYAEKARRVVELTRDIAEILPEFEESLVSVTRRRSVHTVAFHPPCTLQHGQQIRG
KVEHLLGALGVEVRLPTDNHLCCGSAGTYSLTQPKLSYALRDQKLERLQAQEPQVIVSANVGCIAHLQSGTSTPVAHWIE
LVEHMLSV

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=408, Percent_Identity=54.656862745098, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1788576, Length=417, Percent_Identity=22.7817745803357, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786497, Length=238, Percent_Identity=23.109243697479, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 45265; Mature: 45265

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNLADFMRNTPDGDEADGILRNCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVE
CCCCHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCHHHHHCCCCCCCCCCEEEHHHHHC
GAPVTRSTQVHLDRCLTCRNCETTCPSGVQYGRLVEIGRKITEEKVTRPLGQRLMRRMLA
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFVPSSALFTPTMRIGQHFRPLLPRKLRDKVPARQRPLEWPTAKHPRKMLLLAGCVQPSM
HHCCCCHHCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
MPNVNVATARVLDALGIETLIAPDAGCCGAIRLHLGYNDDALDDLRANIDAWWPYVEQGV
CCCCCHHHHHHHHHHCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHCHHHHHHHHHCCC
EAIVMNASGCGATVKEYAHLLRDDPAYAEKARRVVELTRDIAEILPEFEESLVSVTRRRS
CEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VHTVAFHPPCTLQHGQQIRGKVEHLLGALGVEVRLPTDNHLCCGSAGTYSLTQPKLSYAL
CEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCEECCCCCCCCCCCHHHHHHH
RDQKLERLQAQEPQVIVSANVGCIAHLQSGTSTPVAHWIELVEHMLSV
HHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTNLADFMRNTPDGDEADGILRNCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVE
CCCCHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCHHHHHCCCCCCCCCCEEEHHHHHC
GAPVTRSTQVHLDRCLTCRNCETTCPSGVQYGRLVEIGRKITEEKVTRPLGQRLMRRMLA
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFVPSSALFTPTMRIGQHFRPLLPRKLRDKVPARQRPLEWPTAKHPRKMLLLAGCVQPSM
HHCCCCHHCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
MPNVNVATARVLDALGIETLIAPDAGCCGAIRLHLGYNDDALDDLRANIDAWWPYVEQGV
CCCCCHHHHHHHHHHCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHCHHHHHHHHHCCC
EAIVMNASGCGATVKEYAHLLRDDPAYAEKARRVVELTRDIAEILPEFEESLVSVTRRRS
CEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VHTVAFHPPCTLQHGQQIRGKVEHLLGALGVEVRLPTDNHLCCGSAGTYSLTQPKLSYAL
CEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCEECCCCCCCCCCCHHHHHHH
RDQKLERLQAQEPQVIVSANVGCIAHLQSGTSTPVAHWIELVEHMLSV
HHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]