| Definition | Burkholderia xenovorans LB400 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_007951 |
| Length | 4,895,836 |
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The map label for this gene is rlmL [H]
Identifier: 91781842
GI number: 91781842
Start: 512576
End: 513868
Strand: Reverse
Name: rlmL [H]
Synonym: Bxe_A4003
Alternate gene names: 91781842
Gene position: 513868-512576 (Counterclockwise)
Preceding gene: 91781843
Following gene: 91781841
Centisome position: 10.5
GC content: 66.43
Gene sequence:
>1293_bases ATGTCCTTCGATTTCTTCCTCCCTTGCCCACGCGGCCTTGAAGCCTCGCTCGCGGCCGAACTCGCCGAAATTGCCGCCCA GCACCTGAACGGCGCGCCGTTTACAGCCGGCGCACAAGTGCCGGGCGGCGTGCATTTCCGCGGAGGCTGGGCCGCCGGCA TGGCCGCCAACCTGCACTCGCGCCTCGCGAGCCGCGTGCTGCTGAAAGTCGCGCATCGTCCGTATCGCAGCGAGCAGGAC ATTTACGCGCTCGCGGTCGAACAGCGTTGGGAACAATGGTTCTCGGCAAACGAAACGCTGCGGGTGGACGTCACCGCGAT CAAATCGCCGCTGCGCAGCCTGGAATTCACCACACTGCGCGTGAAGGACGCGATCTGCGACCGTCTGCGCGAAGTCAGCG GCGCGCGTCCGAGTATCGACACCGCCATGCCCGACGTGCGCGTGTTCGCGTTTCTCACCGCCACCGACTGCACGCTGTAT CTCGACACCTCGGGCGACCCGCTCTTCAAGCGCGGCTGGCGCCTCGACAAGGGTGCGGCGCCGCTGCGCGAGAATCTGGC CGCGGGCATTCTGCGTCTGACCGGCTGGACCGCCGGCACGCCCCTGTACGACCCCATGTGCGGCAGCGGCACGTTCCTCG CGGAAGCCGCGCAGGTGGCGCTGAATATCGCGCCGGGCGCGGAACGCCGCTTCGGCTTCGAGAAGCTCAAGCAATACGAG GCCAAAACCTGGCAAACGCTCAAGGCCGCCGCGCTCGACGCGAAACACGCCGCGCGTAATTCACGCGCCGATCTGCAGAT CTTCGGCAGCGATATTTCCGGCGACATGCTCGACAAGGCGCGCGCCAACTTCCAGCGCGCCGGCTTGCCGTCCATTCCGC TCAAGCAGCTCGACGCGCGCAACATGACGCCGCCCTCATCCGCGGCGGGCATTCTCGTCGCGAATCCGCCCTATGGCGAA CGGATCGAAGTGCGCGGACGCAATGCGCGCGGCGAGCTTCGTGAAGGCCGCGGCAATCGTGACAGCCGCGAAGACGACGG CTTTCGCCGCGCCCAGGAGGAAACGCCGGACAGCGAATTCTTCCAGTCGCTCGGCGACGCGCTCAAGCAACGCTTCACCG GTTGGCATGCGTTCATCCTGACATCGGACCGCAAGCTGCCCGGCCAGTTGCGTCTGCGTGAATCGACCAAGACACCGCTC TTTAACGGCGCGCTCGAATGCCGGCTGTTCCGCTTCGATCTGATCGCGGGCAGCGTGCGGCAGCGGCCGCAAACCGATAC GCCGGCGGCTTGA
Upstream 100 bases:
>100_bases CGCGCCCAGCCGGCCGCGGCTTGCCGCCAGGCAATCGCGCAGGGCCGCCCACCCCGACCGCGTACAATAGCGCCTTATCC AACGCCTTCCGGCAACCTCA
Downstream 100 bases:
>100_bases CGCACAGCGCAGCGCTTTCGGCGCTGCCGCTGCGAGGCGATAAGAACCGCGAGCCGCGAACCGGCCTCGCGGTTTTTTTC GCCTGCCGCCTGCTTCGGCC
Product: hypothetical protein
Products: NA
Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]
Number of amino acids: Translated: 430; Mature: 429
Protein sequence:
>430_residues MSFDFFLPCPRGLEASLAAELAEIAAQHLNGAPFTAGAQVPGGVHFRGGWAAGMAANLHSRLASRVLLKVAHRPYRSEQD IYALAVEQRWEQWFSANETLRVDVTAIKSPLRSLEFTTLRVKDAICDRLREVSGARPSIDTAMPDVRVFAFLTATDCTLY LDTSGDPLFKRGWRLDKGAAPLRENLAAGILRLTGWTAGTPLYDPMCGSGTFLAEAAQVALNIAPGAERRFGFEKLKQYE AKTWQTLKAAALDAKHAARNSRADLQIFGSDISGDMLDKARANFQRAGLPSIPLKQLDARNMTPPSSAAGILVANPPYGE RIEVRGRNARGELREGRGNRDSREDDGFRRAQEETPDSEFFQSLGDALKQRFTGWHAFILTSDRKLPGQLRLRESTKTPL FNGALECRLFRFDLIAGSVRQRPQTDTPAA
Sequences:
>Translated_430_residues MSFDFFLPCPRGLEASLAAELAEIAAQHLNGAPFTAGAQVPGGVHFRGGWAAGMAANLHSRLASRVLLKVAHRPYRSEQD IYALAVEQRWEQWFSANETLRVDVTAIKSPLRSLEFTTLRVKDAICDRLREVSGARPSIDTAMPDVRVFAFLTATDCTLY LDTSGDPLFKRGWRLDKGAAPLRENLAAGILRLTGWTAGTPLYDPMCGSGTFLAEAAQVALNIAPGAERRFGFEKLKQYE AKTWQTLKAAALDAKHAARNSRADLQIFGSDISGDMLDKARANFQRAGLPSIPLKQLDARNMTPPSSAAGILVANPPYGE RIEVRGRNARGELREGRGNRDSREDDGFRRAQEETPDSEFFQSLGDALKQRFTGWHAFILTSDRKLPGQLRLRESTKTPL FNGALECRLFRFDLIAGSVRQRPQTDTPAA >Mature_429_residues SFDFFLPCPRGLEASLAAELAEIAAQHLNGAPFTAGAQVPGGVHFRGGWAAGMAANLHSRLASRVLLKVAHRPYRSEQDI YALAVEQRWEQWFSANETLRVDVTAIKSPLRSLEFTTLRVKDAICDRLREVSGARPSIDTAMPDVRVFAFLTATDCTLYL DTSGDPLFKRGWRLDKGAAPLRENLAAGILRLTGWTAGTPLYDPMCGSGTFLAEAAQVALNIAPGAERRFGFEKLKQYEA KTWQTLKAAALDAKHAARNSRADLQIFGSDISGDMLDKARANFQRAGLPSIPLKQLDARNMTPPSSAAGILVANPPYGER IEVRGRNARGELREGRGNRDSREDDGFRRAQEETPDSEFFQSLGDALKQRFTGWHAFILTSDRKLPGQLRLRESTKTPLF NGALECRLFRFDLIAGSVRQRPQTDTPAA
Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]
COG id: COG0116
COG function: function code L; Predicted N6-adenine-specific DNA methylase
Gene ontology:
Cell location: Cytoplasm (Potential) [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 THUMP domain [H]
Homologues:
Organism=Escherichia coli, GI1787181, Length=374, Percent_Identity=32.8877005347594, Blast_Score=191, Evalue=6e-50,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002052 - InterPro: IPR000241 - InterPro: IPR017244 - InterPro: IPR019614 - InterPro: IPR004114 [H]
Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]
EC number: =2.1.1.173 [H]
Molecular weight: Translated: 47388; Mature: 47257
Theoretical pI: Translated: 9.61; Mature: 9.61
Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 1.4 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 2.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSFDFFLPCPRGLEASLAAELAEIAAQHLNGAPFTAGAQVPGGVHFRGGWAAGMAANLHS CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHH RLASRVLLKVAHRPYRSEQDIYALAVEQRWEQWFSANETLRVDVTAIKSPLRSLEFTTLR HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCHHEEH VKDAICDRLREVSGARPSIDTAMPDVRVFAFLTATDCTLYLDTSGDPLFKRGWRLDKGAA HHHHHHHHHHHHCCCCCCCHHCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCCCCCC PLRENLAAGILRLTGWTAGTPLYDPMCGSGTFLAEAAQVALNIAPGAERRFGFEKLKQYE HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCHHHHCHHHHHHHH AKTWQTLKAAALDAKHAARNSRADLQIFGSDISGDMLDKARANFQRAGLPSIPLKQLDAR HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCC NMTPPSSAAGILVANPPYGERIEVRGRNARGELREGRGNRDSREDDGFRRAQEETPDSEF CCCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHCCCCCCCCCCCHHHHHHHHCCCHHHH FQSLGDALKQRFTGWHAFILTSDRKLPGQLRLRESTKTPLFNGALECRLFRFDLIAGSVR HHHHHHHHHHHHCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCCEEEEEEHHHHHHHHH QRPQTDTPAA CCCCCCCCCC >Mature Secondary Structure SFDFFLPCPRGLEASLAAELAEIAAQHLNGAPFTAGAQVPGGVHFRGGWAAGMAANLHS CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHH RLASRVLLKVAHRPYRSEQDIYALAVEQRWEQWFSANETLRVDVTAIKSPLRSLEFTTLR HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCHHEEH VKDAICDRLREVSGARPSIDTAMPDVRVFAFLTATDCTLYLDTSGDPLFKRGWRLDKGAA HHHHHHHHHHHHCCCCCCCHHCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCCCCCC PLRENLAAGILRLTGWTAGTPLYDPMCGSGTFLAEAAQVALNIAPGAERRFGFEKLKQYE HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCHHHHCHHHHHHHH AKTWQTLKAAALDAKHAARNSRADLQIFGSDISGDMLDKARANFQRAGLPSIPLKQLDAR HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCC NMTPPSSAAGILVANPPYGERIEVRGRNARGELREGRGNRDSREDDGFRRAQEETPDSEF CCCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHCCCCCCCCCCCHHHHHHHHCCCHHHH FQSLGDALKQRFTGWHAFILTSDRKLPGQLRLRESTKTPLFNGALECRLFRFDLIAGSVR HHHHHHHHHHHHCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCCEEEEEEHHHHHHHHH QRPQTDTPAA CCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA