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The map label for this gene is glyS [H]

Identifier: 91781832

GI number: 91781832

Start: 500975

End: 503074

Strand: Reverse

Name: glyS [H]

Synonym: Bxe_A4013

Alternate gene names: 91781832

Gene position: 503074-500975 (Counterclockwise)

Preceding gene: 91781833

Following gene: 91781831

Centisome position: 10.28

GC content: 65.0

Gene sequence:

>2100_bases
ATGACTCAACCCCATCAAGCTACCCTGCTCGTCGAGCTGCTGACCGAAGAATTGCCGCCGAAAGCGCTCGCGCGTCTCGG
CGACGCCTTCGCTGAAGGCATTGCGCAGCGCCTCGCGGCGCGCGACCTGGTCGAAGGCGAATTGTCGTTCGAACGTTATG
CCACGCCGCGCCGCCTCGCCGTCACGATCAAGAACGTGCGCGCGGTCGCGCCGGAAAAACACGTGCGTGAAAAAGTGCTG
CCGGTTTCCGTCGCGTTGGATAAAGACGGCCAGCCCACCGCGCCGCTCGCCAAGAAACTCGCCGCGCTCGGCTTCCCCGA
TTTTTCGGTGAACGACCTGGAGCGCGCACAGGACGGCAAGGCTGAAGCGTTCTTCCTGCGCTATGCCGCGCCCGGCGCCA
CGCTCGCCGAAGGCCTGCAAAGCGCGCTCGACGAAACGCTCGCCAGGCTGCCCATTCCGAAGGTCATGACGTATCAGCGT
CCGGACGGCACCAACGTGCAATTCGTGCGTCCGGCGCATCGCCTCATCGCGCTGCATGGCGAACAGATCGTGCCGGTGAG
CGCGCTCGGCATCGACGCCGACGACACCACGCTCGGCCACCGTTTCCTCTCCGAAGGTTTCGTGCAGATCCAGCACGCGG
ATTCGTACGCCGAGACGCTGCTGCACAAAGGCCGCGTAGTGGCGAATTTCTCCGATCGAAAGGAGACCATCCGCACGCAT
CTGCTCGCGCAAGCAAACGGCGACCAGGTGGTGATGCCGGAATCGCTGCTGGACGAAGTGACGTCGCTGGTCGAATGGCC
GGTGGTTTATGCGTGCCGTTTCGAGGACGAATTCCTGCAAGTGCCGCAGGAATGCCTGATCCTCACCATGCAGACCAACC
AGAAATACTTCGCGCTGACCGATGCGAACGGCAAGCTGCGCTCGCGCTTCCTGATCGTGTCGAATATCGAAACGTCCACG
CCGGGCGATATCGTGGAAGGCAACGAGCGCGTGGTGCGTCCGCGTCTGGCCGACGCGAAGTTCTTCTTCGAGCAGGACAA
GAAGAAGACGCTCGCCGATCGCGTGCCGCTGCTCGCGAACGTCGTGTATCACAACAAGCTGGGCTCGGCACTGCAACGCG
TGGAGCGCGTCGAAGCGCTGGCCGGCGCGATTGCCGGACTGATCGGCGCGGACGTGGCGCTCGCGAAGCGCGCCGCGCGC
CTCGCCAAGGCCGATCTGATCACCGACATGGTCGGCGAATTCCCCGAGCTGCAAGGCACGATGGGCACCTACTACGCGCG
CCACGACGGCGAGCCGGAAGAAGTCGCGCTCGCGTGCTCGGAACACTATCAGCCGCGTTTCTCCGGCGACGCATTGCCCG
CTACCGCAACCGGCACCGTCGTCGCGCTGGCCGACAAGCTCGAAACGCTGGTCGGCATCTGGGGCATCGGCCTGCAACCC
ACCGGCGAGAAAGACCCGTTCGCGTTGCGCCGTCACGCGCTCGGCGTGCTGCGCATTCTGGTCGAGAAGCAACTGCCGGT
CGACTTCGTCGAACTGCTGCGCAACGCTTACGCGCAATTCGCCGCGGTGCCGAACGTCGTCGATTCGACGCAAGCCATTT
ACGAGTTCAGCATGGACCGGCTGCGCGGTCTGCTGCGCGAGCGCGGCTATGCGCCGGGCGAAATCGACGCGGTGCTGGCG
CTGAACCCCACGCGCCTGGACGACATCGTCGCGCGTCTGGATGCAGTGCGCGAATTCGCGTCGCTGGCTGAAGCGGCTTC
GCTCGCGGCGGCCAACAAGCGCATCTCGAACATCCTGAAGAAGTCGGAAGGCGCGGCGACCGGCGGTGTGCAGGTCACGC
TGCTCGTCGAAGCGGCGGAAAAAGCCCTGCATGCGCAACTCGAACAGGTCGCGCCGCGCGTGCAGTCGCAACTGGCCGCG
CGCGACTATACGGGTGCGCTGACCGCGCTCGCCGCGTTGCGCGAACCGGTCGACACGTTCTTCAACGACGTGATGGTCAA
CGCCGAAGATCCCGCGTTGCGCGCCAACCGTCTGGCCTTGCTCGGCGCACTGCATCAGCAGATGAACTGCGTCGCCGACA
TTTCCCGACTCGCCGCCTGA

Upstream 100 bases:

>100_bases
CGGCCTCACGACTGACGAACAGGACGCCGCCATGCCCGCATGGGCGCCGCCGCTGAAAGTCGAACGCAAGATCGACCAGG
ACTGACGAGAACATCAAGAA

Downstream 100 bases:

>100_bases
GCCCCGCGCCATGCCGACCAAAAAAGTGGTGATCCTCGACCGCGACGGCGTGATCAACGTCGACTCCGACGCGTTCATCA
AGTCGCCGGACGAATGGGTC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 699; Mature: 698

Protein sequence:

>699_residues
MTQPHQATLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLVEGELSFERYATPRRLAVTIKNVRAVAPEKHVREKVL
PVSVALDKDGQPTAPLAKKLAALGFPDFSVNDLERAQDGKAEAFFLRYAAPGATLAEGLQSALDETLARLPIPKVMTYQR
PDGTNVQFVRPAHRLIALHGEQIVPVSALGIDADDTTLGHRFLSEGFVQIQHADSYAETLLHKGRVVANFSDRKETIRTH
LLAQANGDQVVMPESLLDEVTSLVEWPVVYACRFEDEFLQVPQECLILTMQTNQKYFALTDANGKLRSRFLIVSNIETST
PGDIVEGNERVVRPRLADAKFFFEQDKKKTLADRVPLLANVVYHNKLGSALQRVERVEALAGAIAGLIGADVALAKRAAR
LAKADLITDMVGEFPELQGTMGTYYARHDGEPEEVALACSEHYQPRFSGDALPATATGTVVALADKLETLVGIWGIGLQP
TGEKDPFALRRHALGVLRILVEKQLPVDFVELLRNAYAQFAAVPNVVDSTQAIYEFSMDRLRGLLRERGYAPGEIDAVLA
LNPTRLDDIVARLDAVREFASLAEAASLAAANKRISNILKKSEGAATGGVQVTLLVEAAEKALHAQLEQVAPRVQSQLAA
RDYTGALTALAALREPVDTFFNDVMVNAEDPALRANRLALLGALHQQMNCVADISRLAA

Sequences:

>Translated_699_residues
MTQPHQATLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLVEGELSFERYATPRRLAVTIKNVRAVAPEKHVREKVL
PVSVALDKDGQPTAPLAKKLAALGFPDFSVNDLERAQDGKAEAFFLRYAAPGATLAEGLQSALDETLARLPIPKVMTYQR
PDGTNVQFVRPAHRLIALHGEQIVPVSALGIDADDTTLGHRFLSEGFVQIQHADSYAETLLHKGRVVANFSDRKETIRTH
LLAQANGDQVVMPESLLDEVTSLVEWPVVYACRFEDEFLQVPQECLILTMQTNQKYFALTDANGKLRSRFLIVSNIETST
PGDIVEGNERVVRPRLADAKFFFEQDKKKTLADRVPLLANVVYHNKLGSALQRVERVEALAGAIAGLIGADVALAKRAAR
LAKADLITDMVGEFPELQGTMGTYYARHDGEPEEVALACSEHYQPRFSGDALPATATGTVVALADKLETLVGIWGIGLQP
TGEKDPFALRRHALGVLRILVEKQLPVDFVELLRNAYAQFAAVPNVVDSTQAIYEFSMDRLRGLLRERGYAPGEIDAVLA
LNPTRLDDIVARLDAVREFASLAEAASLAAANKRISNILKKSEGAATGGVQVTLLVEAAEKALHAQLEQVAPRVQSQLAA
RDYTGALTALAALREPVDTFFNDVMVNAEDPALRANRLALLGALHQQMNCVADISRLAA
>Mature_698_residues
TQPHQATLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLVEGELSFERYATPRRLAVTIKNVRAVAPEKHVREKVLP
VSVALDKDGQPTAPLAKKLAALGFPDFSVNDLERAQDGKAEAFFLRYAAPGATLAEGLQSALDETLARLPIPKVMTYQRP
DGTNVQFVRPAHRLIALHGEQIVPVSALGIDADDTTLGHRFLSEGFVQIQHADSYAETLLHKGRVVANFSDRKETIRTHL
LAQANGDQVVMPESLLDEVTSLVEWPVVYACRFEDEFLQVPQECLILTMQTNQKYFALTDANGKLRSRFLIVSNIETSTP
GDIVEGNERVVRPRLADAKFFFEQDKKKTLADRVPLLANVVYHNKLGSALQRVERVEALAGAIAGLIGADVALAKRAARL
AKADLITDMVGEFPELQGTMGTYYARHDGEPEEVALACSEHYQPRFSGDALPATATGTVVALADKLETLVGIWGIGLQPT
GEKDPFALRRHALGVLRILVEKQLPVDFVELLRNAYAQFAAVPNVVDSTQAIYEFSMDRLRGLLRERGYAPGEIDAVLAL
NPTRLDDIVARLDAVREFASLAEAASLAAANKRISNILKKSEGAATGGVQVTLLVEAAEKALHAQLEQVAPRVQSQLAAR
DYTGALTALAALREPVDTFFNDVMVNAEDPALRANRLALLGALHQQMNCVADISRLAA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=697, Percent_Identity=43.6154949784792, Blast_Score=530, Evalue=1e-151,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 76157; Mature: 76026

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPHQATLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLVEGELSFERYATPRRLA
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEE
VTIKNVRAVAPEKHVREKVLPVSVALDKDGQPTAPLAKKLAALGFPDFSVNDLERAQDGK
EEEHHHHHCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCC
AEAFFLRYAAPGATLAEGLQSALDETLARLPIPKVMTYQRPDGTNVQFVRPAHRLIALHG
CEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCEEEECHHHHHHHCCC
EQIVPVSALGIDADDTTLGHRFLSEGFVQIQHADSYAETLLHKGRVVANFSDRKETIRTH
CCEEEEEEECCCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHCCCEEECCHHHHHHHHHH
LLAQANGDQVVMPESLLDEVTSLVEWPVVYACRFEDEFLQVPQECLILTMQTNQKYFALT
HHHCCCCCEEECCHHHHHHHHHHHHCCEEEEEECCHHHHHCCHHHEEEEEECCCEEEEEE
DANGKLRSRFLIVSNIETSTPGDIVEGNERVVRPRLADAKFFFEQDKKKTLADRVPLLAN
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
VVYHNKLGSALQRVERVEALAGAIAGLIGADVALAKRAARLAKADLITDMVGEFPELQGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
MGTYYARHDGEPEEVALACSEHYQPRFSGDALPATATGTVVALADKLETLVGIWGIGLQP
HHHEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
TGEKDPFALRRHALGVLRILVEKQLPVDFVELLRNAYAQFAAVPNVVDSTQAIYEFSMDR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LRGLLRERGYAPGEIDAVLALNPTRLDDIVARLDAVREFASLAEAASLAAANKRISNILK
HHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSEGAATGGVQVTLLVEAAEKALHAQLEQVAPRVQSQLAARDYTGALTALAALREPVDTF
HCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNDVMVNAEDPALRANRLALLGALHQQMNCVADISRLAA
HHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQPHQATLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLVEGELSFERYATPRRLA
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEE
VTIKNVRAVAPEKHVREKVLPVSVALDKDGQPTAPLAKKLAALGFPDFSVNDLERAQDGK
EEEHHHHHCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCC
AEAFFLRYAAPGATLAEGLQSALDETLARLPIPKVMTYQRPDGTNVQFVRPAHRLIALHG
CEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCEEEECHHHHHHHCCC
EQIVPVSALGIDADDTTLGHRFLSEGFVQIQHADSYAETLLHKGRVVANFSDRKETIRTH
CCEEEEEEECCCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHCCCEEECCHHHHHHHHHH
LLAQANGDQVVMPESLLDEVTSLVEWPVVYACRFEDEFLQVPQECLILTMQTNQKYFALT
HHHCCCCCEEECCHHHHHHHHHHHHCCEEEEEECCHHHHHCCHHHEEEEEECCCEEEEEE
DANGKLRSRFLIVSNIETSTPGDIVEGNERVVRPRLADAKFFFEQDKKKTLADRVPLLAN
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
VVYHNKLGSALQRVERVEALAGAIAGLIGADVALAKRAARLAKADLITDMVGEFPELQGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
MGTYYARHDGEPEEVALACSEHYQPRFSGDALPATATGTVVALADKLETLVGIWGIGLQP
HHHEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
TGEKDPFALRRHALGVLRILVEKQLPVDFVELLRNAYAQFAAVPNVVDSTQAIYEFSMDR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LRGLLRERGYAPGEIDAVLALNPTRLDDIVARLDAVREFASLAEAASLAAANKRISNILK
HHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSEGAATGGVQVTLLVEAAEKALHAQLEQVAPRVQSQLAARDYTGALTALAALREPVDTF
HCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNDVMVNAEDPALRANRLALLGALHQQMNCVADISRLAA
HHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA