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The map label for this gene is paaF [C]

Identifier: 91781810

GI number: 91781810

Start: 476577

End: 477347

Strand: Direct

Name: paaF [C]

Synonym: Bxe_A4035

Alternate gene names: 91781810

Gene position: 476577-477347 (Clockwise)

Preceding gene: 91781809

Following gene: 91781812

Centisome position: 9.73

GC content: 65.89

Gene sequence:

>771_bases
ATGACGATGGATATTCTGGTCGAGCGCGCCGACGGCGTGCTGACGATTGCCTTCAACCGGCCCGACAAGAAAAACGCGAT
CACGGCCGCCATGTACCAGACGATGGCCGACGCGCTGGTTCAAGCGCACGGCGACGCCGCGGTGCGCGCCGTCCTGATTC
GCGGCAGCGCGGGCATTTTCAGCGCCGGCAACGATCTCGAAGATTTCATGAAGGCGCCGCCAATGGGCGAAAACGCGCCC
GTATTCCAGTTCCTGCGCGCGATCAGTTCGGCGGAGAAGCCGGTGGTGGCGTCGGTAGCGGGCCCGGCGGTGGGCATCGG
CACGACCTTGCTGCTGCATTGCGATCTGGTCTACGTGGCCGACACGGCGAGCTTTTCGCTGCCGTTCGCGCAGCTCGGCC
TGTGCCCTGAAGCGGCGTCGAGCCTGCTGTTGCAGCGGGTGAGCGGCTACCAGGCGGCGGCGGAGAAGCTGCTGCTCGGC
GAAGCGTTCGACGCCGCGGAGGCGCAACGCATGGGCTTCGTGAATCGCGTGCTGCCCGCCGCCGAGGTCGACGCGTTCGC
CGCTTCGCAAGCCGCGAAGCTGGCCGCGTTGCCGGCCTCGTCGCTGCGTGTGACGAAAAGCCTGATGAAGCGCGCGAGCT
ACCAGGAACTGCAAACGCAGATGTCGGAAGAAGCCGTGCATTTCGGCAAGATGCTGCTCGCGCCCGAAGCACGCGAGGCG
TTCAAGGCGTTCTTCGAGAAGCGCAAGCCGGACTTCCGGCAGTTCGACTGA

Upstream 100 bases:

>100_bases
TCGGGGCGCCCCCTTGGGGCGACCCGTTAGGCACGCGCAACGGTTCGCAGGCCATCCGGCTTGCGAACCGTTTTTACATT
CTGAGCAGGAGGACGAGGAG

Downstream 100 bases:

>100_bases
GCGGCGGCCCTGAGCGCTTGAACCACTTCGGGCGCGAGCGCTCGGGCGTAGAACGGACCGGCCGGCTTGGGCGGGCTCCA
GCTTTTTGCGCGGCTGGGGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTMDILVERADGVLTIAFNRPDKKNAITAAMYQTMADALVQAHGDAAVRAVLIRGSAGIFSAGNDLEDFMKAPPMGENAP
VFQFLRAISSAEKPVVASVAGPAVGIGTTLLLHCDLVYVADTASFSLPFAQLGLCPEAASSLLLQRVSGYQAAAEKLLLG
EAFDAAEAQRMGFVNRVLPAAEVDAFAASQAAKLAALPASSLRVTKSLMKRASYQELQTQMSEEAVHFGKMLLAPEAREA
FKAFFEKRKPDFRQFD

Sequences:

>Translated_256_residues
MTMDILVERADGVLTIAFNRPDKKNAITAAMYQTMADALVQAHGDAAVRAVLIRGSAGIFSAGNDLEDFMKAPPMGENAP
VFQFLRAISSAEKPVVASVAGPAVGIGTTLLLHCDLVYVADTASFSLPFAQLGLCPEAASSLLLQRVSGYQAAAEKLLLG
EAFDAAEAQRMGFVNRVLPAAEVDAFAASQAAKLAALPASSLRVTKSLMKRASYQELQTQMSEEAVHFGKMLLAPEAREA
FKAFFEKRKPDFRQFD
>Mature_255_residues
TMDILVERADGVLTIAFNRPDKKNAITAAMYQTMADALVQAHGDAAVRAVLIRGSAGIFSAGNDLEDFMKAPPMGENAPV
FQFLRAISSAEKPVVASVAGPAVGIGTTLLLHCDLVYVADTASFSLPFAQLGLCPEAASSLLLQRVSGYQAAAEKLLLGE
AFDAAEAQRMGFVNRVLPAAEVDAFAASQAAKLAALPASSLRVTKSLMKRASYQELQTQMSEEAVHFGKMLLAPEAREAF
KAFFEKRKPDFRQFD

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=251, Percent_Identity=33.8645418326693, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI260275230, Length=251, Percent_Identity=33.8645418326693, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI260274832, Length=251, Percent_Identity=33.8645418326693, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=27.2373540856031, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=27.2373540856031, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI25453481, Length=257, Percent_Identity=26.8482490272374, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI51538573, Length=257, Percent_Identity=26.8482490272374, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=27.1255060728745, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI4757966, Length=262, Percent_Identity=27.4809160305344, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI51538581, Length=262, Percent_Identity=27.4809160305344, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=26.5873015873016, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI70995211, Length=239, Percent_Identity=30.5439330543933, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI221136756, Length=259, Percent_Identity=23.1660231660232, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI221136753, Length=259, Percent_Identity=23.1660231660232, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI221307494, Length=259, Percent_Identity=23.1660231660232, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI62530384, Length=246, Percent_Identity=24.7967479674797, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI68989263, Length=197, Percent_Identity=30.9644670050761, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI116235487, Length=215, Percent_Identity=26.046511627907, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=27.6264591439689, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI295842266, Length=244, Percent_Identity=24.1803278688525, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=30.4347826086957, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=32.156862745098, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788597, Length=266, Percent_Identity=29.6992481203008, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI221142681, Length=268, Percent_Identity=32.8358208955224, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082183, Length=253, Percent_Identity=26.4822134387352, Blast_Score=84, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=34.2629482071713, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17549921, Length=232, Percent_Identity=34.4827586206897, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17554946, Length=256, Percent_Identity=32.03125, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=28.6290322580645, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=247, Percent_Identity=28.7449392712551, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=190, Percent_Identity=27.3684210526316, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=262, Percent_Identity=26.3358778625954, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24654903, Length=251, Percent_Identity=32.6693227091633, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=32.5757575757576, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI20129971, Length=242, Percent_Identity=28.5123966942149, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24653477, Length=242, Percent_Identity=28.5123966942149, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19922422, Length=265, Percent_Identity=26.4150943396226, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24650670, Length=208, Percent_Identity=28.8461538461538, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21357171, Length=257, Percent_Identity=25.2918287937743, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19920382, Length=185, Percent_Identity=28.1081081081081, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27376; Mature: 27245

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMDILVERADGVLTIAFNRPDKKNAITAAMYQTMADALVQAHGDAAVRAVLIRGSAGIF
CCHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCC
SAGNDLEDFMKAPPMGENAPVFQFLRAISSAEKPVVASVAGPAVGIGTTLLLHCDLVYVA
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCEEE
DTASFSLPFAQLGLCPEAASSLLLQRVSGYQAAAEKLLLGEAFDAAEAQRMGFVNRVLPA
CCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
AEVDAFAASQAAKLAALPASSLRVTKSLMKRASYQELQTQMSEEAVHFGKMLLAPEAREA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FKAFFEKRKPDFRQFD
HHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TMDILVERADGVLTIAFNRPDKKNAITAAMYQTMADALVQAHGDAAVRAVLIRGSAGIF
CHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCC
SAGNDLEDFMKAPPMGENAPVFQFLRAISSAEKPVVASVAGPAVGIGTTLLLHCDLVYVA
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCEEE
DTASFSLPFAQLGLCPEAASSLLLQRVSGYQAAAEKLLLGEAFDAAEAQRMGFVNRVLPA
CCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
AEVDAFAASQAAKLAALPASSLRVTKSLMKRASYQELQTQMSEEAVHFGKMLLAPEAREA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FKAFFEKRKPDFRQFD
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA