Click here to switch to the map view.

The map label for this gene is maiA [H]

Identifier: 91781705

GI number: 91781705

Start: 356750

End: 357394

Strand: Direct

Name: maiA [H]

Synonym: Bxe_A4141

Alternate gene names: 91781705

Gene position: 356750-357394 (Clockwise)

Preceding gene: 91781704

Following gene: 91781707

Centisome position: 7.29

GC content: 61.09

Gene sequence:

>645_bases
ATGAAGCTCTATAGCTATTTCCGTAGCTCGGCGTCCTATCGCGTGCGCATTGCGCTGAACGTGAAGAATCTGCCGTACGA
GTACGTGCCGGTGCACCTCGTGCGCGAGGGCGGCGAGCAACTGAAGCCGGAATACCGCAAGGTGAATGTGGACTGCGTCG
TGCCCACGCTCGTCGAAGGCAATGACGTGATGCCGCAGTCGCTCGCGATCATCGAGTATCTGGAAGAAACGCATCCCGAG
CCGCCGCTTCTGCCCAAAGCGCCGGTCGATCGTGCGTATGTGCGGGCGCTGGCGTTGCAGGTGGCGTGCGAGATCCATCC
GCTGAACAATCTGCGCGTGCTGAAGTATCTGAAGCACACGCTGGGTGTCGACGACTATGCGAAGGACGCGTGGTACCGGC
ACTGGGTGGAAGCGGGGTTCGCCACGTTCGAAGCGCATCTGGCCGGCGATTCACGCACCGGCAAGCTGTGTTTCGGCGAT
ACGCCGACAATCGCCGATACCTGTCTGATTCCGCAGGTGTTCAACGCGCAGCGCTTCAAGGTCGACACGGCGAAATTTCC
GACGATCCAGCGCATCTACGATCACGCAATGCAACTCGACGCGTTTGCCCGTGCGGCGCCAGACGTGCAGCCCGACGCCG
AGTAA

Upstream 100 bases:

>100_bases
CGGTGGTGCCGGGCGATCTGATGAAGGGCGGCGTGGACGGGCTCGGCGAATTCAGCGTGCGCGTCGTCTGACGGCTGGTT
TGGGGGGGGAGACAGACAAC

Downstream 100 bases:

>100_bases
GCCGGCCTCGTCCATCGGCGAGGCAGGATGAAAAAAGGGCACTCCGCGGAGTGCCCTTTTTGCTCTCTGAGCCGCAAACG
CCGTGGCGTTCAGCTGCCCA

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKLYSYFRSSASYRVRIALNVKNLPYEYVPVHLVREGGEQLKPEYRKVNVDCVVPTLVEGNDVMPQSLAIIEYLEETHPE
PPLLPKAPVDRAYVRALALQVACEIHPLNNLRVLKYLKHTLGVDDYAKDAWYRHWVEAGFATFEAHLAGDSRTGKLCFGD
TPTIADTCLIPQVFNAQRFKVDTAKFPTIQRIYDHAMQLDAFARAAPDVQPDAE

Sequences:

>Translated_214_residues
MKLYSYFRSSASYRVRIALNVKNLPYEYVPVHLVREGGEQLKPEYRKVNVDCVVPTLVEGNDVMPQSLAIIEYLEETHPE
PPLLPKAPVDRAYVRALALQVACEIHPLNNLRVLKYLKHTLGVDDYAKDAWYRHWVEAGFATFEAHLAGDSRTGKLCFGD
TPTIADTCLIPQVFNAQRFKVDTAKFPTIQRIYDHAMQLDAFARAAPDVQPDAE
>Mature_214_residues
MKLYSYFRSSASYRVRIALNVKNLPYEYVPVHLVREGGEQLKPEYRKVNVDCVVPTLVEGNDVMPQSLAIIEYLEETHPE
PPLLPKAPVDRAYVRALALQVACEIHPLNNLRVLKYLKHTLGVDDYAKDAWYRHWVEAGFATFEAHLAGDSRTGKLCFGD
TPTIADTCLIPQVFNAQRFKVDTAKFPTIQRIYDHAMQLDAFARAAPDVQPDAE

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=211, Percent_Identity=45.4976303317536, Blast_Score=173, Evalue=8e-44,
Organism=Homo sapiens, GI22202622, Length=160, Percent_Identity=44.375, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI194440732, Length=211, Percent_Identity=34.5971563981043, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17551302, Length=211, Percent_Identity=44.5497630331754, Blast_Score=162, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17510461, Length=212, Percent_Identity=44.3396226415094, Blast_Score=155, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17556142, Length=212, Percent_Identity=40.0943396226415, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24645375, Length=211, Percent_Identity=44.5497630331754, Blast_Score=170, Evalue=8e-43,
Organism=Drosophila melanogaster, GI21355859, Length=211, Percent_Identity=44.5497630331754, Blast_Score=169, Evalue=9e-43,
Organism=Drosophila melanogaster, GI45553325, Length=211, Percent_Identity=44.5497630331754, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI21355857, Length=212, Percent_Identity=41.0377358490566, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24289; Mature: 24289

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYSYFRSSASYRVRIALNVKNLPYEYVPVHLVREGGEQLKPEYRKVNVDCVVPTLVEG
CCHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHEECCCHHCCCHHHEEEEEEEEEEEECC
NDVMPQSLAIIEYLEETHPEPPLLPKAPVDRAYVRALALQVACEIHPLNNLRVLKYLKHT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHH
LGVDDYAKDAWYRHWVEAGFATFEAHLAGDSRTGKLCFGDTPTIADTCLIPQVFNAQRFK
CCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHCCCEEE
VDTAKFPTIQRIYDHAMQLDAFARAAPDVQPDAE
ECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLYSYFRSSASYRVRIALNVKNLPYEYVPVHLVREGGEQLKPEYRKVNVDCVVPTLVEG
CCHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHEECCCHHCCCHHHEEEEEEEEEEEECC
NDVMPQSLAIIEYLEETHPEPPLLPKAPVDRAYVRALALQVACEIHPLNNLRVLKYLKHT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHH
LGVDDYAKDAWYRHWVEAGFATFEAHLAGDSRTGKLCFGDTPTIADTCLIPQVFNAQRFK
CCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHCCCEEE
VDTAKFPTIQRIYDHAMQLDAFARAAPDVQPDAE
ECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]