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The map label for this gene is dctD [H]

Identifier: 91781651

GI number: 91781651

Start: 301581

End: 302933

Strand: Direct

Name: dctD [H]

Synonym: Bxe_A4195

Alternate gene names: 91781651

Gene position: 301581-302933 (Clockwise)

Preceding gene: 91781650

Following gene: 91781653

Centisome position: 6.16

GC content: 66.22

Gene sequence:

>1353_bases
ATGCTGAATCACGGCCTGCAAGTGCTGTATATCGAAGACGACGAACTGGTGCGGCGCGCCAGCGTGCAGAGTCTGCAACT
GGCGGGCTTCGAGGTGATCGGCCATGCGTCGGTGGAATCGGCGGCGAAGACGATCGGCGCGGACTTCTCCGGCGTGATCG
TCAGCGACATTCGCTTGCCGGGCGCAAGCGGCCTCGACCTGCTTGCGCAATGCCACGAGCGCGCGCCCGACGTGCCGGTG
ATTCTGGTGACGGGCCACGGCGACATTTCAATGGCCGTTCAGGCCATGCGCGACGGCGCGTACGACTTCATCGAGAAGCC
GTTCGCGTCGGAACGTTTGATCGAAACCGTGCGGCGCGCGTTGGAGCGCCGCAAGCTGGTGCTGGAAAACCTCGCGTTGC
GCCGTGAACTGGCGGAGCAGAGTTCGGTGGCGCCGCGGATCATCGGCAGGAGTCCGGCGATCGAACAGGTGCGGCGGTTG
ATTGCCAATATCGCGCCGACGGACGCCTCCGTGCTGATCAACGGCGACACCGGCGCGGGCAAGGAGCTGATCGCGCGCAG
TCTGCATGAACTGTCGCCGCGGCGTGACAAGCCGTTTATCGCAGTGAACTGTGGCGCGCTGCCGGAGCCGATGTTCGAAT
CGGAAATGTTCGGCTACGAGCCGGGCGCATTCACCGGCGCGGCGAAACGGCGGATCGGCAAGCTCGAGCATGCGTCGGGC
GGCACGCTGTTTCTGGACGAAATCGAGAGCATGCCGCTCGCACTACAGGTCAAGCTGCTGCGCGTGCTGCAGGACGGCGT
GCTGGAACGGCTCGGCTCGAATCAGCCGATTCGGGTGAATTGCCGCGTGGTCGCCGCAGCCAAGGGCGACATGGCCGAGC
ACGTCGCGGACGGCTCGTTCCGGCGCGATCTGCTGTACCGGCTGAACGTGGTGACGATTGCGCTGCCGCCGCTCGGTGAG
CGTCGCGAGGACATCGTGCCGCTTTTCGAACACTTCCTGCTGGACGCGGCGGTGCGTTATCAACGCCCGGCGCCGATCCT
CACAGACCGTCAGCGCGCGGGCCTGATGCAGCGCGACTGGCCGGGCAACGTGCGGGAACTGCGCAACGCCGCTGACCGCT
TCGTGCTCGGCGTCGCCGACGATCCCGTCATGTCTTTCGGCGACGACGGCGCGGCCGCGCAGCCTTTGAAAGAGCGCGTC
GAACAATTCGAGCGGGCGGTGATCGCGGAGGCGTTGGAGCAGGCCGGCGGCGTGGTCGCCGTCGCGGCAGACAGGCTGCA
ACTCGGCAAGGCGACGCTCTACGAGAAGATCAAGCGATATCGCCTCGCGGCCAAAGGGGAAGGGGAGCGGTGA

Upstream 100 bases:

>100_bases
GCGGTGCATTATTCGTGTTGACGCTGCGCCGCGCGCGCGTGCAGACGAGCCACACACCGGACCCGCTGACAACGGGCTCC
TGAATCACACGGAAAACGCC

Downstream 100 bases:

>100_bases
ACAAATGAAAACGCCGGCGTTGGGACGTGACGCCGGCGTGTGCGTGAAGCGAGGGCCCTGGAACCAGATTGGAGCGAGCG
GGCCGCGGGCCAGGCCGCGC

Product: two component, sigma-54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLNHGLQVLYIEDDELVRRASVQSLQLAGFEVIGHASVESAAKTIGADFSGVIVSDIRLPGASGLDLLAQCHERAPDVPV
ILVTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRALERRKLVLENLALRRELAEQSSVAPRIIGRSPAIEQVRRL
IANIAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEHASG
GTLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMAEHVADGSFRRDLLYRLNVVTIALPPLGE
RREDIVPLFEHFLLDAAVRYQRPAPILTDRQRAGLMQRDWPGNVRELRNAADRFVLGVADDPVMSFGDDGAAAQPLKERV
EQFERAVIAEALEQAGGVVAVAADRLQLGKATLYEKIKRYRLAAKGEGER

Sequences:

>Translated_450_residues
MLNHGLQVLYIEDDELVRRASVQSLQLAGFEVIGHASVESAAKTIGADFSGVIVSDIRLPGASGLDLLAQCHERAPDVPV
ILVTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRALERRKLVLENLALRRELAEQSSVAPRIIGRSPAIEQVRRL
IANIAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEHASG
GTLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMAEHVADGSFRRDLLYRLNVVTIALPPLGE
RREDIVPLFEHFLLDAAVRYQRPAPILTDRQRAGLMQRDWPGNVRELRNAADRFVLGVADDPVMSFGDDGAAAQPLKERV
EQFERAVIAEALEQAGGVVAVAADRLQLGKATLYEKIKRYRLAAKGEGER
>Mature_450_residues
MLNHGLQVLYIEDDELVRRASVQSLQLAGFEVIGHASVESAAKTIGADFSGVIVSDIRLPGASGLDLLAQCHERAPDVPV
ILVTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRALERRKLVLENLALRRELAEQSSVAPRIIGRSPAIEQVRRL
IANIAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEHASG
GTLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMAEHVADGSFRRDLLYRLNVVTIALPPLGE
RREDIVPLFEHFLLDAAVRYQRPAPILTDRQRAGLMQRDWPGNVRELRNAADRFVLGVADDPVMSFGDDGAAAQPLKERV
EQFERAVIAEALEQAGGVVAVAADRLQLGKATLYEKIKRYRLAAKGEGER

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=450, Percent_Identity=39.1111111111111, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=37.2844827586207, Blast_Score=291, Evalue=8e-80,
Organism=Escherichia coli, GI1790437, Length=456, Percent_Identity=36.8421052631579, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1788905, Length=446, Percent_Identity=36.5470852017937, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI87082117, Length=321, Percent_Identity=41.7445482866044, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=41.25, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1789087, Length=328, Percent_Identity=43.9024390243902, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI87081872, Length=256, Percent_Identity=44.921875, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1786524, Length=356, Percent_Identity=38.2022471910112, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI87082152, Length=317, Percent_Identity=41.0094637223975, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1787583, Length=336, Percent_Identity=35.4166666666667, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI87081858, Length=369, Percent_Identity=29.810298102981, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789828, Length=243, Percent_Identity=35.3909465020576, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49275; Mature: 49275

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNHGLQVLYIEDDELVRRASVQSLQLAGFEVIGHASVESAAKTIGADFSGVIVSDIRLP
CCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCCCCEEEEEEECC
GASGLDLLAQCHERAPDVPVILVTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRA
CCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHH
LERRKLVLENLALRRELAEQSSVAPRIIGRSPAIEQVRRLIANIAPTDASVLINGDTGAG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCC
KELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEHASG
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCC
GTLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMAEHVADGSF
CEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHCCCHH
RRDLLYRLNVVTIALPPLGERREDIVPLFEHFLLDAAVRYQRPAPILTDRQRAGLMQRDW
HHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCC
PGNVRELRNAADRFVLGVADDPVMSFGDDGAAAQPLKERVEQFERAVIAEALEQAGGVVA
CCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEE
VAADRLQLGKATLYEKIKRYRLAAKGEGER
EEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLNHGLQVLYIEDDELVRRASVQSLQLAGFEVIGHASVESAAKTIGADFSGVIVSDIRLP
CCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCCCCEEEEEEECC
GASGLDLLAQCHERAPDVPVILVTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRA
CCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHH
LERRKLVLENLALRRELAEQSSVAPRIIGRSPAIEQVRRLIANIAPTDASVLINGDTGAG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCC
KELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEHASG
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCC
GTLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMAEHVADGSF
CEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHCCCHH
RRDLLYRLNVVTIALPPLGERREDIVPLFEHFLLDAAVRYQRPAPILTDRQRAGLMQRDW
HHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCC
PGNVRELRNAADRFVLGVADDPVMSFGDDGAAAQPLKERVEQFERAVIAEALEQAGGVVA
CCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEE
VAADRLQLGKATLYEKIKRYRLAAKGEGER
EEHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]