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The map label for this gene is dctA [H]

Identifier: 91781649

GI number: 91781649

Start: 298227

End: 299507

Strand: Direct

Name: dctA [H]

Synonym: Bxe_A4197

Alternate gene names: 91781649

Gene position: 298227-299507 (Clockwise)

Preceding gene: 91781648

Following gene: 91781650

Centisome position: 6.09

GC content: 62.06

Gene sequence:

>1281_bases
GTGAAGAAACCTATCCACAAAGTGCTGTACGTCCAGGTGATCGTGGCGATCATCATCGGTATCGGGCTCGGGCATTTCTA
CCCGGATCTCGCCGTCGACATGAAGCCGCTCGGCGACGGCTTCATCAAGCTGATCAAGATGGTGATCGGGCCGATCATTT
TCTGTACAGTGGTGACAGGTATTGCCGGCATGGAGGATATGAAGAAAGTCGGCCGGGTGGGCGGCAAGGCGCTGCTGTAT
TTCGAAATCGTCTCGACCTTCGCACTGGTGCTCGGCCTTATCGCCACGCATGTACTGAAGCCGGGCGTCGGCTTCAACAT
CGATCCGGCCACGCTCGACGGCAAGGCCGTCGCCTCGTACGCCGCGAAAGCGCACGGGCAGACCACGGTCGACTTCCTGA
TGCACATCATTCCGGATACGCTCGTCTCGGCGTTCGCGCAGGGCGAGATCCTGCAGATCCTGCTGATCGCGCTGCTGTTC
GGCGCGGTGCTCGCCACGGCCGGTGAGAAGGGCAAGGTCGTGACGGGCTTTATCGACGGGCTCTCGCATGTGCTGTTCGG
CATCGTGCGCATCATCACCAAGCTGGCGCCGATCGGCGCGTTCGGTGCGATGGCCTTCACGATCGGCAAGTACGGTATCG
GCTCGCTGTTGCCGATGCTCAAGCTGATCGGCACGTTCTATCTGACCTCGGTCGTGTTCGTGGTGGTGGTGCTCGGCATC
ATCGCGCGTGCGGTGGGCTTCAACATCCTGCGCTTCGTTGCGTACATCAAGGAAGAGATGCTGATCGTGCTCGGCACGAG
CTCGTCCGAAGCGGCGCTCCCGCAACTGATGCTGAAGCTCGAACGGCTCGGCTGCTCGCGTTCGGTGGTGGGCCTCGTGG
TGCCGACCGGTTACTCGTTCAATCTCGACGGCACCAACATCTATATGACGATGGCCGTGCTGTTCATCGCGCAGGCGACC
AACACCGACCTGACATGGACGCAGCAGCTCACGCTGCTCGCGGTCACCATGCTGACCTCGAAGGGCGCGAGCGGCGTGAC
GGGCGCGGGCTTCATCACGCTGGCGGCGACGCTTGCCGTGGTGCCGACCATTCCGCTGTCGGGCATGGTGCTGATTCTGG
GTATCGACCGCTTCATGAGCGAATGCCGCGCGCTGACCAACATTGTCGGCAACGGTGTGGCGACGGTGGTCGTGTCGGCG
TGGGAAAAGGAACTGGACCGCAGCAAGCTGAACGCCGCCTTGCGCGGCGACGTGGCGATCAAAGAGCCGGCCGGCGTTTA
A

Upstream 100 bases:

>100_bases
CTTGTCGCGGTCTGATGCCGTGCACAGGCGTGACGACTATTCGTTCGCATGATGCGCAGCGTGGCAGGCCATTGAGCTCA
TCCACATCGCAGGAACCATC

Downstream 100 bases:

>100_bases
ACGGCGCATCGGCAACCCGCTCCAGCGCAACCCCGACGGCCGCGGATACCAGCGTATGAAACCGGTGTCCGCGCGCCGAA
CCTCCGTGCCGTCGGCCGAC

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKKPIHKVLYVQVIVAIIIGIGLGHFYPDLAVDMKPLGDGFIKLIKMVIGPIIFCTVVTGIAGMEDMKKVGRVGGKALLY
FEIVSTFALVLGLIATHVLKPGVGFNIDPATLDGKAVASYAAKAHGQTTVDFLMHIIPDTLVSAFAQGEILQILLIALLF
GAVLATAGEKGKVVTGFIDGLSHVLFGIVRIITKLAPIGAFGAMAFTIGKYGIGSLLPMLKLIGTFYLTSVVFVVVVLGI
IARAVGFNILRFVAYIKEEMLIVLGTSSSEAALPQLMLKLERLGCSRSVVGLVVPTGYSFNLDGTNIYMTMAVLFIAQAT
NTDLTWTQQLTLLAVTMLTSKGASGVTGAGFITLAATLAVVPTIPLSGMVLILGIDRFMSECRALTNIVGNGVATVVVSA
WEKELDRSKLNAALRGDVAIKEPAGV

Sequences:

>Translated_426_residues
MKKPIHKVLYVQVIVAIIIGIGLGHFYPDLAVDMKPLGDGFIKLIKMVIGPIIFCTVVTGIAGMEDMKKVGRVGGKALLY
FEIVSTFALVLGLIATHVLKPGVGFNIDPATLDGKAVASYAAKAHGQTTVDFLMHIIPDTLVSAFAQGEILQILLIALLF
GAVLATAGEKGKVVTGFIDGLSHVLFGIVRIITKLAPIGAFGAMAFTIGKYGIGSLLPMLKLIGTFYLTSVVFVVVVLGI
IARAVGFNILRFVAYIKEEMLIVLGTSSSEAALPQLMLKLERLGCSRSVVGLVVPTGYSFNLDGTNIYMTMAVLFIAQAT
NTDLTWTQQLTLLAVTMLTSKGASGVTGAGFITLAATLAVVPTIPLSGMVLILGIDRFMSECRALTNIVGNGVATVVVSA
WEKELDRSKLNAALRGDVAIKEPAGV
>Mature_426_residues
MKKPIHKVLYVQVIVAIIIGIGLGHFYPDLAVDMKPLGDGFIKLIKMVIGPIIFCTVVTGIAGMEDMKKVGRVGGKALLY
FEIVSTFALVLGLIATHVLKPGVGFNIDPATLDGKAVASYAAKAHGQTTVDFLMHIIPDTLVSAFAQGEILQILLIALLF
GAVLATAGEKGKVVTGFIDGLSHVLFGIVRIITKLAPIGAFGAMAFTIGKYGIGSLLPMLKLIGTFYLTSVVFVVVVLGI
IARAVGFNILRFVAYIKEEMLIVLGTSSSEAALPQLMLKLERLGCSRSVVGLVVPTGYSFNLDGTNIYMTMAVLFIAQAT
NTDLTWTQQLTLLAVTMLTSKGASGVTGAGFITLAATLAVVPTIPLSGMVLILGIDRFMSECRALTNIVGNGVATVVVSA
WEKELDRSKLNAALRGDVAIKEPAGV

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=460, Percent_Identity=26.0869565217391, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI4827012, Length=275, Percent_Identity=30.1818181818182, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI66773030, Length=412, Percent_Identity=25, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI21314632, Length=400, Percent_Identity=24.5, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI40254478, Length=460, Percent_Identity=23.9130434782609, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI194239697, Length=456, Percent_Identity=22.5877192982456, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI5032093, Length=397, Percent_Identity=26.448362720403, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI262359914, Length=402, Percent_Identity=25.3731343283582, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI223468564, Length=269, Percent_Identity=27.8810408921933, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI223468566, Length=269, Percent_Identity=27.8810408921933, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI301601644, Length=167, Percent_Identity=27.5449101796407, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789947, Length=425, Percent_Identity=62.1176470588235, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1790514, Length=426, Percent_Identity=37.5586854460094, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1788024, Length=407, Percent_Identity=21.6216216216216, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=399, Percent_Identity=26.3157894736842, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71996953, Length=467, Percent_Identity=25.0535331905782, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71983099, Length=442, Percent_Identity=24.4343891402715, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71983106, Length=442, Percent_Identity=24.4343891402715, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI193206505, Length=476, Percent_Identity=23.9495798319328, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI193206654, Length=480, Percent_Identity=22.0833333333333, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17541374, Length=435, Percent_Identity=22.0689655172414, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583025, Length=420, Percent_Identity=22.6190476190476, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17137668, Length=420, Percent_Identity=22.6190476190476, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24583023, Length=420, Percent_Identity=22.6190476190476, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281360483, Length=470, Percent_Identity=24.0425531914894, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137666, Length=470, Percent_Identity=24.0425531914894, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281360481, Length=470, Percent_Identity=24.0425531914894, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 44910; Mature: 44910

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPIHKVLYVQVIVAIIIGIGLGHFYPDLAVDMKPLGDGFIKLIKMVIGPIIFCTVVTG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IAGMEDMKKVGRVGGKALLYFEIVSTFALVLGLIATHVLKPGVGFNIDPATLDGKAVASY
HCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
AAKAHGQTTVDFLMHIIPDTLVSAFAQGEILQILLIALLFGAVLATAGEKGKVVTGFIDG
HHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
LSHVLFGIVRIITKLAPIGAFGAMAFTIGKYGIGSLLPMLKLIGTFYLTSVVFVVVVLGI
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARAVGFNILRFVAYIKEEMLIVLGTSSSEAALPQLMLKLERLGCSRSVVGLVVPTGYSF
HHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCEEE
NLDGTNIYMTMAVLFIAQATNTDLTWTQQLTLLAVTMLTSKGASGVTGAGFITLAATLAV
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VPTIPLSGMVLILGIDRFMSECRALTNIVGNGVATVVVSAWEKELDRSKLNAALRGDVAI
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEE
KEPAGV
CCCCCC
>Mature Secondary Structure
MKKPIHKVLYVQVIVAIIIGIGLGHFYPDLAVDMKPLGDGFIKLIKMVIGPIIFCTVVTG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IAGMEDMKKVGRVGGKALLYFEIVSTFALVLGLIATHVLKPGVGFNIDPATLDGKAVASY
HCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
AAKAHGQTTVDFLMHIIPDTLVSAFAQGEILQILLIALLFGAVLATAGEKGKVVTGFIDG
HHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
LSHVLFGIVRIITKLAPIGAFGAMAFTIGKYGIGSLLPMLKLIGTFYLTSVVFVVVVLGI
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARAVGFNILRFVAYIKEEMLIVLGTSSSEAALPQLMLKLERLGCSRSVVGLVVPTGYSF
HHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCEEE
NLDGTNIYMTMAVLFIAQATNTDLTWTQQLTLLAVTMLTSKGASGVTGAGFITLAATLAV
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VPTIPLSGMVLILGIDRFMSECRALTNIVGNGVATVVVSAWEKELDRSKLNAALRGDVAI
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEE
KEPAGV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA