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The map label for this gene is metH [H]

Identifier: 91781603

GI number: 91781603

Start: 254162

End: 256879

Strand: Reverse

Name: metH [H]

Synonym: Bxe_A4243

Alternate gene names: 91781603

Gene position: 256879-254162 (Counterclockwise)

Preceding gene: 91781604

Following gene: 91781601

Centisome position: 5.25

GC content: 62.36

Gene sequence:

>2718_bases
ATGACCGATCACACCTTGCGCCTTGCCGGCCTCGAGCCGTTCAACGTCACGTCCGGGACGCTCTTCATCAACGTGGGCGA
ACGCACCAACGTGACCGGCTCGAAGGCGTTTGCGCGAATGATCCTGAACGACCAGTTCGACGATGCGATCGCGGTCGCGC
GCCAGCAGGTCGAAAACGGCGCGCAGATCATCGACGTCAACATGGACGAAGCGATGCTCGATTCGAAAGCGGCGATGGTG
CGCTTCATGAATCTGATCGCGTCGGAACCGGATATCGCGCGCGTGCCGATCATGATCGACTCGTCGAAGTGGGAAGTGAT
CGAAGCGGGCCTGAAGTGCGTGCAGGGCAAGGCCATCGTCAACTCGATCTCGCTGAAGGAAGGCGAAGAAGCGTTCCGCC
ACCACGCGAACCTGATTCGCCGCTACGGCGCGGCCGCCGTGGTGATGGCCTTCGACGAACAGGGCCAGGCCGACACCTTC
GAGCGCAAGACGCAGATCTGCAAGCGCTCGTATGACTTCCTCGTCAACGAAGTCGGCTTCCCGCCGGAAGACATCATCTT
CGATCCGAACATCTTCGCGATCGCGACCGGCATCGAAGAGCACAACAACTACGCGGTCGACTTCATCAACGCAACGCGCT
GGATCAAGCAGAATCTGCCGTACGCGAAGGTGAGCGGCGGCGTGTCCAACGTGTCGTTCTCGTTTCGCGGCAATGATCCG
GTGCGCGAAGCGATCCACACCGTGTTCCTCTACCACGCGATCCAGGCGGGCATGGACATGGGCATCGTCAACGCGGGCCA
GCTCGGCGTGTATGCCGACCTCGATCCGGAGTTGCGCGAACGCGTGGAAGACGTCGTGCTGAACCGCCGTGAAGACGGCA
CCGACCGGCTGCTCGAAATCGCCGACAAGTTCAAGACCGGCGCCGCGAAGAAGGAAGAGAATCTGGAGTGGCGCAACCAG
CCGGTCGAAAAACGTCTGTCGCATGCGCTCGTGCATGGCATCACCAACTTCATCGTCGAAGACACCGAGGAAGTGCGTGC
GAAGATCGCCGCCGAAGGCGGCCGTCCGATCAATGTGATCGAAGGCCCGCTGATGGACGGCATGAACATCGTCGGCGACC
TGTTCGGGCAGGGCAAGATGTTCCTGCCGCAAGTGGTGAAGTCGGCGCGCGTCATGAAGCAGGCGGTCGCGCATCTGATT
CCGTATATCGAGGAAGAGAAGAAGCAGCTCGCCGAAGCCGGCGCCGACGTGCGTGCGAAGGGCAAGATCGTCATCGCCAC
GGTGAAGGGCGACGTGCACGATATCGGCAAGAACATCGTCTCCGTGGTGCTTCAGTGCAATAACTTCGAAGTGGTCAACA
TGGGCGTGATGGTCTCGTGCAACGACATTCTCGCCAAGGCGAAGGTCGAAGGCGCGGACATCATCGGGCTCTCCGGGTTG
ATCACGCCGAGCCTCGAAGAGATGGCGTACGTCGCATCGGAAATGCAGCGCGACGACTACTTCCGCATCAAGAAGATTCC
GCTGCTGATCGGCGGCGCCACCACCTCGCGCGTGCACACCGCGGTGAAGATCGCGCCGCATTACGAAGGGCCGGTGGTCT
ACGTGCCGGACGCGTCGCGCTCGGTTTCCGTCGCGTCGAGCCTGCTGTCCGACGAGGGCGCGGCGAAGTACCTCGACGAA
CTCAAGTCCGATTACGACCGGATCCGCGACCAGCACGCCAACAAGAAAGCACTGCCTATGGTCACGCTGGCCGAAGCCCG
CGCCAACAAGACCAGGGTCGACTGGGCGAGCTATCAGCCGGTCAAGCCGAAGTTCATCGGCCGCCGCGTGTTCAGGAACT
TCGATCTGGCCGAACTGGCGACCTATATCGACTGGGGTCCGTTCTTCCAGACGTGGGACCTGGCGGGCCCCTATCCGGCC
ATCCTGAACGACGAGATCGTCGGCGAATCGGCGCGGCGCGTGTTCTCGGACGGCAAGTCGATGCTCGCGCGTCTGATCCA
GGGCCGCTGGCTCCAGGCGAACGGCGTGATCGCGCTACTGCCCGCCAACACGGTGAACGACGACGACATCGAAATCTACA
CGGACGAATCGCGCTCGGAAGTCGCGCTCACGTGGCGCAATCTGCGCCAGCAAAGCGTGCGTCCGGTGGTGGACGGCGTG
ATGCGGCCGAACCGGTCGCTCGCTGACTTCATCGCGCCGAAAGACTCGGGCGTGGCCGACTACATCGGCATGTTCGCGGT
GACGGCGGGACTCGGCGTCGACGTGAAGGAAAAGCAGTTCGAAAAAGATCACGACGACTACAGCGCGATCATGCTGAAGG
CGCTCGCCGACCGTTTCGCCGAAGCCTTCGCCGAAGCGCTGCACGCCCGCGTGCGGCGCGACCTGTGGGGTTACGCAAAT
GACGAAACGCTCTCCACCGACGAACTGATCGCGGAAAAGTACCGCGGCATCCGTCCGGCGCCCGGCTATCCGGCCTGCCC
GGATCACCTGGTGAAGCGCGACATGTTCAATGTGCTGCACGCCACCGAAATCGGCATGAGCGTGACCGAATCGCTGGCCA
TGCTGCCGGCGGCGAGCGTCTCCGGCTTCTATCTGGCACATCCGGACAGCACCTATTTCTCGGTCGGCAAGATCGGCCAG
GACCAGCTGGAAGACTACGCGAGGCGCATGTCGCTCTCGAAAACGGATGCGGAGCGAGCCCTCGCGCCTTTGCTGTAA

Upstream 100 bases:

>100_bases
CGCGAACAATGCCCGCACTTCCGCCAATGGTACGACCGCATCGTGACGCTGCCTGCCCTGCAGGCGCAGCAACCCAATAT
TGAATATACGAGCTAACGCC

Downstream 100 bases:

>100_bases
GCGCCGGGCGGCGAGGCGTGGCGGGAAACCCACTATATCGGGCATGAAAGACTGAATATTTGCGGCAACGTAATTTTCGG
CTTTGTAGACTGAGGCGGCT

Product: methionine synthase (B12-dependent)

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 905; Mature: 904

Protein sequence:

>905_residues
MTDHTLRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNDQFDDAIAVARQQVENGAQIIDVNMDEAMLDSKAAMV
RFMNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEEAFRHHANLIRRYGAAAVVMAFDEQGQADTF
ERKTQICKRSYDFLVNEVGFPPEDIIFDPNIFAIATGIEEHNNYAVDFINATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVVLNRREDGTDRLLEIADKFKTGAAKKEENLEWRNQ
PVEKRLSHALVHGITNFIVEDTEEVRAKIAAEGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLI
PYIEEEKKQLAEAGADVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVSCNDILAKAKVEGADIIGLSGL
ITPSLEEMAYVASEMQRDDYFRIKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASSLLSDEGAAKYLDE
LKSDYDRIRDQHANKKALPMVTLAEARANKTRVDWASYQPVKPKFIGRRVFRNFDLAELATYIDWGPFFQTWDLAGPYPA
ILNDEIVGESARRVFSDGKSMLARLIQGRWLQANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNLRQQSVRPVVDGV
MRPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDVKEKQFEKDHDDYSAIMLKALADRFAEAFAEALHARVRRDLWGYAN
DETLSTDELIAEKYRGIRPAPGYPACPDHLVKRDMFNVLHATEIGMSVTESLAMLPAASVSGFYLAHPDSTYFSVGKIGQ
DQLEDYARRMSLSKTDAERALAPLL

Sequences:

>Translated_905_residues
MTDHTLRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNDQFDDAIAVARQQVENGAQIIDVNMDEAMLDSKAAMV
RFMNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEEAFRHHANLIRRYGAAAVVMAFDEQGQADTF
ERKTQICKRSYDFLVNEVGFPPEDIIFDPNIFAIATGIEEHNNYAVDFINATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVVLNRREDGTDRLLEIADKFKTGAAKKEENLEWRNQ
PVEKRLSHALVHGITNFIVEDTEEVRAKIAAEGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLI
PYIEEEKKQLAEAGADVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVSCNDILAKAKVEGADIIGLSGL
ITPSLEEMAYVASEMQRDDYFRIKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASSLLSDEGAAKYLDE
LKSDYDRIRDQHANKKALPMVTLAEARANKTRVDWASYQPVKPKFIGRRVFRNFDLAELATYIDWGPFFQTWDLAGPYPA
ILNDEIVGESARRVFSDGKSMLARLIQGRWLQANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNLRQQSVRPVVDGV
MRPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDVKEKQFEKDHDDYSAIMLKALADRFAEAFAEALHARVRRDLWGYAN
DETLSTDELIAEKYRGIRPAPGYPACPDHLVKRDMFNVLHATEIGMSVTESLAMLPAASVSGFYLAHPDSTYFSVGKIGQ
DQLEDYARRMSLSKTDAERALAPLL
>Mature_904_residues
TDHTLRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNDQFDDAIAVARQQVENGAQIIDVNMDEAMLDSKAAMVR
FMNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEEAFRHHANLIRRYGAAAVVMAFDEQGQADTFE
RKTQICKRSYDFLVNEVGFPPEDIIFDPNIFAIATGIEEHNNYAVDFINATRWIKQNLPYAKVSGGVSNVSFSFRGNDPV
REAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVVLNRREDGTDRLLEIADKFKTGAAKKEENLEWRNQP
VEKRLSHALVHGITNFIVEDTEEVRAKIAAEGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIP
YIEEEKKQLAEAGADVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVSCNDILAKAKVEGADIIGLSGLI
TPSLEEMAYVASEMQRDDYFRIKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASSLLSDEGAAKYLDEL
KSDYDRIRDQHANKKALPMVTLAEARANKTRVDWASYQPVKPKFIGRRVFRNFDLAELATYIDWGPFFQTWDLAGPYPAI
LNDEIVGESARRVFSDGKSMLARLIQGRWLQANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNLRQQSVRPVVDGVM
RPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDVKEKQFEKDHDDYSAIMLKALADRFAEAFAEALHARVRRDLWGYAND
ETLSTDELIAEKYRGIRPAPGYPACPDHLVKRDMFNVLHATEIGMSVTESLAMLPAASVSGFYLAHPDSTYFSVGKIGQD
QLEDYARRMSLSKTDAERALAPLL

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=918, Percent_Identity=53.4858387799564, Blast_Score=961, Evalue=0.0,
Organism=Escherichia coli, GI1790450, Length=905, Percent_Identity=61.4364640883978, Blast_Score=1092, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17535405, Length=912, Percent_Identity=54.1666666666667, Blast_Score=944, Evalue=0.0,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 100208; Mature: 100077

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHTLRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNDQFDDAIAVARQQVENG
CCCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCC
AQIIDVNMDEAMLDSKAAMVRFMNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIV
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHH
NSISLKEGEEAFRHHANLIRRYGAAAVVMAFDEQGQADTFERKTQICKRSYDFLVNEVGF
HHCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PPEDIIFDPNIFAIATGIEEHNNYAVDFINATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
CCHHEEECCCEEEEEECCHHCCCEEEEEHHHHHHHHHCCCCEEECCCCCEEEEEECCCCH
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVVLNRREDGTDRLLEI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHH
ADKFKTGAAKKEENLEWRNQPVEKRLSHALVHGITNFIVEDTEEVRAKIAAEGGRPINVI
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEE
EGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPYIEEEKKQLAEAGADVRAK
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVSCNDILAKAKVEGADIIGLSGL
CCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEHHHHHHHHHCCCCCEEEECCC
ITPSLEEMAYVASEMQRDDYFRIKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASR
CCCCHHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHHEEEEEEECCCCCCEEECCCCCC
SVSVASSLLSDEGAAKYLDELKSDYDRIRDQHANKKALPMVTLAEARANKTRVDWASYQP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCCCCCCCCC
VKPKFIGRRVFRNFDLAELATYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDGKS
CCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHCHHHHHHHHCHHH
MLARLIQGRWLQANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNLRQQSVRPVVDGV
HHHHHHCCCEEECCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
MRPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDVKEKQFEKDHDDYSAIMLKALADRFA
HCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
EAFAEALHARVRRDLWGYANDETLSTDELIAEKYRGIRPAPGYPACPDHLVKRDMFNVLH
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
ATEIGMSVTESLAMLPAASVSGFYLAHPDSTYFSVGKIGQDQLEDYARRMSLSKTDAERA
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHH
LAPLL
HHCCC
>Mature Secondary Structure 
TDHTLRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNDQFDDAIAVARQQVENG
CCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCC
AQIIDVNMDEAMLDSKAAMVRFMNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIV
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHH
NSISLKEGEEAFRHHANLIRRYGAAAVVMAFDEQGQADTFERKTQICKRSYDFLVNEVGF
HHCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PPEDIIFDPNIFAIATGIEEHNNYAVDFINATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
CCHHEEECCCEEEEEECCHHCCCEEEEEHHHHHHHHHCCCCEEECCCCCEEEEEECCCCH
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVVLNRREDGTDRLLEI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHH
ADKFKTGAAKKEENLEWRNQPVEKRLSHALVHGITNFIVEDTEEVRAKIAAEGGRPINVI
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEE
EGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPYIEEEKKQLAEAGADVRAK
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVSCNDILAKAKVEGADIIGLSGL
CCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEHHHHHHHHHCCCCCEEEECCC
ITPSLEEMAYVASEMQRDDYFRIKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASR
CCCCHHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHHEEEEEEECCCCCCEEECCCCCC
SVSVASSLLSDEGAAKYLDELKSDYDRIRDQHANKKALPMVTLAEARANKTRVDWASYQP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCCCCCCCCC
VKPKFIGRRVFRNFDLAELATYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDGKS
CCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHCHHHHHHHHCHHH
MLARLIQGRWLQANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNLRQQSVRPVVDGV
HHHHHHCCCEEECCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
MRPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDVKEKQFEKDHDDYSAIMLKALADRFA
HCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
EAFAEALHARVRRDLWGYANDETLSTDELIAEKYRGIRPAPGYPACPDHLVKRDMFNVLH
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
ATEIGMSVTESLAMLPAASVSGFYLAHPDSTYFSVGKIGQDQLEDYARRMSLSKTDAERA
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHH
LAPLL
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]