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The map label for this gene is ghrA [H]

Identifier: 91781576

GI number: 91781576

Start: 225734

End: 226675

Strand: Reverse

Name: ghrA [H]

Synonym: Bxe_A4269

Alternate gene names: 91781576

Gene position: 226675-225734 (Counterclockwise)

Preceding gene: 91781577

Following gene: 91781575

Centisome position: 4.63

GC content: 66.99

Gene sequence:

>942_bases
ATGAAAATCCTCTTCTACATGCCGCATGCCGACGCCACCGCGTGGCTGAACGACTTCGCCCGCGCGCTGCCGGCGGCCGA
TCTGCGTGAATGGCAGCCGGGCGACACCGCGCCGGCCGATTTCGCAGTGGTGTGGCGTCCGCCGCGCGAGATGCTGGCCG
GACGCGACGATCTGCACGCCATCTTCAATCTGGGCGCGGGCGTCGACGCGATCCTCGCGCTGGAACACGCGCAACCCGGC
ACGTTGCCGCGCAACGCGCAGTTGATCCGCCTGGAAGACACCGGCATGGCGCCGCAAATGGCCGAGTACGTCGCGCATGC
GGTGCTGCGCTATCTGCGCCGTTTCGACGAGTATCAGACGCTGCAAGCCGAGCGCCGCTGGCAAGTGCTCGAGCCGCATC
CGCGTGAAACCTTTACCGTCGGCGTACTGGGACTTGGCGTGCTCGGCGCGCATGTCGCCCAGGCGGTGGCGGCGTTCGGT
GTGCCGGTGCGCGGCTACAGCCGCAGCCCGCGGCAGATCGACGGCATTCAGACGTTTGCCGGCGAAGCGCAGTTCGACGC
GTTTCTCGACGGCGTGAAAGTGCTGGTGAATCTGCTGCCGCATACGCCGGATACCGGCGACATGCTGAACGCACGCACCT
TCGCGAAACTCGCACGCGGCGCGTATCTGATCAACGTGGCGCGCGGCGGGCATCTGGTCGAGCAGGATCTGCTCGACGCG
CTCGCGAGCGGCCAGCTCACCGCCGCCACGCTCGACGTGTTCCGCGAAGAACCGCTGCCGCCGGATCATCCATTCTGGCG
CGAGCCGCGCATCACGATCACGCCGCACGTGTCCGCGCTGACGTTGCGCGAGGAAAGCGTCGTCCAGGTCGCGCGGAAAA
TCACGGCGCTCGCACGAGGCGATACAGTTGGCGGAGTGGTGAATCTGGAACGCGGATACTGA

Upstream 100 bases:

>100_bases
TCATGCAGCAAGTGTCGTCCGCAGCGTTGCAGCTGTCGCATCGGTCACGCGGTGGCTTCTCCGTCACCCGCCGCGACCTC
GCAGGCGCCTTCCAGACCGT

Downstream 100 bases:

>100_bases
ACACAGCCAATCCCATTGAAGGAGCGCCGCCACGCGCGGCGTCAACGAGTTGCATGGAGACAGATCATGGCCCTACCCCA
GCAAGTGAAAATCGTCGAAG

Product: putative 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKILFYMPHADATAWLNDFARALPAADLREWQPGDTAPADFAVVWRPPREMLAGRDDLHAIFNLGAGVDAILALEHAQPG
TLPRNAQLIRLEDTGMAPQMAEYVAHAVLRYLRRFDEYQTLQAERRWQVLEPHPRETFTVGVLGLGVLGAHVAQAVAAFG
VPVRGYSRSPRQIDGIQTFAGEAQFDAFLDGVKVLVNLLPHTPDTGDMLNARTFAKLARGAYLINVARGGHLVEQDLLDA
LASGQLTAATLDVFREEPLPPDHPFWREPRITITPHVSALTLREESVVQVARKITALARGDTVGGVVNLERGY

Sequences:

>Translated_313_residues
MKILFYMPHADATAWLNDFARALPAADLREWQPGDTAPADFAVVWRPPREMLAGRDDLHAIFNLGAGVDAILALEHAQPG
TLPRNAQLIRLEDTGMAPQMAEYVAHAVLRYLRRFDEYQTLQAERRWQVLEPHPRETFTVGVLGLGVLGAHVAQAVAAFG
VPVRGYSRSPRQIDGIQTFAGEAQFDAFLDGVKVLVNLLPHTPDTGDMLNARTFAKLARGAYLINVARGGHLVEQDLLDA
LASGQLTAATLDVFREEPLPPDHPFWREPRITITPHVSALTLREESVVQVARKITALARGDTVGGVVNLERGY
>Mature_313_residues
MKILFYMPHADATAWLNDFARALPAADLREWQPGDTAPADFAVVWRPPREMLAGRDDLHAIFNLGAGVDAILALEHAQPG
TLPRNAQLIRLEDTGMAPQMAEYVAHAVLRYLRRFDEYQTLQAERRWQVLEPHPRETFTVGVLGLGVLGAHVAQAVAAFG
VPVRGYSRSPRQIDGIQTFAGEAQFDAFLDGVKVLVNLLPHTPDTGDMLNARTFAKLARGAYLINVARGGHLVEQDLLDA
LASGQLTAATLDVFREEPLPPDHPFWREPRITITPHVSALTLREESVVQVARKITALARGDTVGGVVNLERGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=273, Percent_Identity=30.03663003663, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI6912396, Length=256, Percent_Identity=29.6875, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI145580578, Length=269, Percent_Identity=28.6245353159851, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4557499, Length=269, Percent_Identity=28.6245353159851, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI61743967, Length=273, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4557497, Length=273, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI145580575, Length=269, Percent_Identity=28.6245353159851, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI87081824, Length=313, Percent_Identity=49.5207667731629, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI87082289, Length=144, Percent_Identity=31.9444444444444, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=220, Percent_Identity=30.9090909090909, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324055, Length=126, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322116, Length=200, Percent_Identity=27.5, Blast_Score=66, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320925, Length=200, Percent_Identity=27.5, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI28571528, Length=173, Percent_Identity=32.9479768786127, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24585516, Length=146, Percent_Identity=32.1917808219178, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28574286, Length=179, Percent_Identity=31.2849162011173, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921140, Length=184, Percent_Identity=32.0652173913043, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585514, Length=179, Percent_Identity=29.608938547486, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28574282, Length=179, Percent_Identity=29.608938547486, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI45552429, Length=179, Percent_Identity=29.608938547486, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28574284, Length=179, Percent_Identity=29.608938547486, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45551003, Length=179, Percent_Identity=29.608938547486, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34443; Mature: 34443

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILFYMPHADATAWLNDFARALPAADLREWQPGDTAPADFAVVWRPPREMLAGRDDLHA
CEEEEEECCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEECCHHHHHCCCHHHHH
IFNLGAGVDAILALEHAQPGTLPRNAQLIRLEDTGMAPQMAEYVAHAVLRYLRRFDEYQT
HHHCCCCHHHHEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
LQAERRWQVLEPHPRETFTVGVLGLGVLGAHVAQAVAAFGVPVRGYSRSPRQIDGIQTFA
HHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHHC
GEAQFDAFLDGVKVLVNLLPHTPDTGDMLNARTFAKLARGAYLINVARGGHLVEQDLLDA
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHH
LASGQLTAATLDVFREEPLPPDHPFWREPRITITPHVSALTLREESVVQVARKITALARG
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHCC
DTVGGVVNLERGY
CCCCCEEEECCCC
>Mature Secondary Structure
MKILFYMPHADATAWLNDFARALPAADLREWQPGDTAPADFAVVWRPPREMLAGRDDLHA
CEEEEEECCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEECCHHHHHCCCHHHHH
IFNLGAGVDAILALEHAQPGTLPRNAQLIRLEDTGMAPQMAEYVAHAVLRYLRRFDEYQT
HHHCCCCHHHHEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
LQAERRWQVLEPHPRETFTVGVLGLGVLGAHVAQAVAAFGVPVRGYSRSPRQIDGIQTFA
HHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHHC
GEAQFDAFLDGVKVLVNLLPHTPDTGDMLNARTFAKLARGAYLINVARGGHLVEQDLLDA
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHH
LASGQLTAATLDVFREEPLPPDHPFWREPRITITPHVSALTLREESVVQVARKITALARG
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHCC
DTVGGVVNLERGY
CCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA