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The map label for this gene is 91781570

Identifier: 91781570

GI number: 91781570

Start: 221170

End: 222141

Strand: Direct

Name: 91781570

Synonym: Bxe_A4275

Alternate gene names: NA

Gene position: 221170-222141 (Clockwise)

Preceding gene: 91781568

Following gene: 91781578

Centisome position: 4.52

GC content: 64.09

Gene sequence:

>972_bases
ATGCTTGCGTGGGCTGTCCCACCTCGCGCGGACACATTGGCCTCCTACAATGCAGTGACTCCAGATCACAAGAGAGACGT
CATGGCCTTCGAGAATTTCACGCCCTTTCGCGTCGCCGTGGGCGATGTCGATATTTTTGGAGTGAAGGGCGGAGCGGGCC
CGCCTCTTCTTTTGTTGCACGGTCATCCGCAGTCGCATCTGATCTGGCGGAAGTGCGCTGCGCGACTGGCCAAACATTTC
ACCGTCATCGCCACCGATCTGCGCGGCTACGGCGCATCGGGCAAGCCGCCGAGCGACGCCGAACATACGCCGTATTCCAA
GCGCGTGATGGCGGCCGATCAGGTCGCCGTGATGCGTCATTTCGGCTTCGAACGTTTTCTCGTGTGCGCACACGACCGCG
GCGCACGCGTCGCGCATCGAATGGCGCTCGATCATCCCGACGCCGTCGAGCGTCTGATGCTGCTCGATATCGCGCCGACG
CTCGCAATGTACGAGGCCACCGACCGCACCTTCGCGACGCACTACTTCCACTGGTTCTTCCTCATCCAGCCGGAGCCGCT
GCCCGAGACGCTGATCGGCGCGAACCCGGCGGCTTATGTCGATGCGGTGATGGGCGGCCGTCATGCGGGTCTGGCGCCGT
TCGATCCCGCCGCGCTCGACGCCTATCGCTCGGCGCTCGCCCAACCAGGCGCCGTGCACGCGATGTGCGAGGACTACCGC
GCGTCGGCGAGCATCGACCTGGAGCACGATCGGGCCGATATCGAGCGCGGTCACAAGATCGGCTGCCCGCTGCGCGTGTT
GTGGGGCGACAAGGGCGTGATCGAGAAGTGCTTCGACGCGCTGGCCGAATGGCGTCATGTGGCGCGCGACGTGAGTGGCC
GCGCGCTGCCTTGCGGACACTACATACCCGAAGAAGCATCGGACGAACTGGTCGCCGAAATGCTGTCTTTTTTCGAAGCG
GTCGAGCAGTAG

Upstream 100 bases:

>100_bases
CCATCCGATAATTTAAGAACGATCGTTCGATTATAGGCGATGCGCGGCGCTCGCGTCCGGCCGCTGCGTTTGGAACAGTT
GGAAGGAGTGTTCAGGTTCT

Downstream 100 bases:

>100_bases
GTCCCGTTCGTCCGCTGCGGTCGTGCGTGAAGCCGCGCTCGCCGTCGTTCCGCCGTAGCGGCGTGCCACAATGCTACGGA
TTCCCGAGTAACTGGAATCC

Product: putative hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MLAWAVPPRADTLASYNAVTPDHKRDVMAFENFTPFRVAVGDVDIFGVKGGAGPPLLLLHGHPQSHLIWRKCAARLAKHF
TVIATDLRGYGASGKPPSDAEHTPYSKRVMAADQVAVMRHFGFERFLVCAHDRGARVAHRMALDHPDAVERLMLLDIAPT
LAMYEATDRTFATHYFHWFFLIQPEPLPETLIGANPAAYVDAVMGGRHAGLAPFDPAALDAYRSALAQPGAVHAMCEDYR
ASASIDLEHDRADIERGHKIGCPLRVLWGDKGVIEKCFDALAEWRHVARDVSGRALPCGHYIPEEASDELVAEMLSFFEA
VEQ

Sequences:

>Translated_323_residues
MLAWAVPPRADTLASYNAVTPDHKRDVMAFENFTPFRVAVGDVDIFGVKGGAGPPLLLLHGHPQSHLIWRKCAARLAKHF
TVIATDLRGYGASGKPPSDAEHTPYSKRVMAADQVAVMRHFGFERFLVCAHDRGARVAHRMALDHPDAVERLMLLDIAPT
LAMYEATDRTFATHYFHWFFLIQPEPLPETLIGANPAAYVDAVMGGRHAGLAPFDPAALDAYRSALAQPGAVHAMCEDYR
ASASIDLEHDRADIERGHKIGCPLRVLWGDKGVIEKCFDALAEWRHVARDVSGRALPCGHYIPEEASDELVAEMLSFFEA
VEQ
>Mature_323_residues
MLAWAVPPRADTLASYNAVTPDHKRDVMAFENFTPFRVAVGDVDIFGVKGGAGPPLLLLHGHPQSHLIWRKCAARLAKHF
TVIATDLRGYGASGKPPSDAEHTPYSKRVMAADQVAVMRHFGFERFLVCAHDRGARVAHRMALDHPDAVERLMLLDIAPT
LAMYEATDRTFATHYFHWFFLIQPEPLPETLIGANPAAYVDAVMGGRHAGLAPFDPAALDAYRSALAQPGAVHAMCEDYR
ASASIDLEHDRADIERGHKIGCPLRVLWGDKGVIEKCFDALAEWRHVARDVSGRALPCGHYIPEEASDELVAEMLSFFEA
VEQ

Specific function: Catalyzes the hydrolytic defluorination of fluoroacetate to produce glycolate. Has low activity towards chloroacetate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777840, Length=278, Percent_Identity=27.6978417266187, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI13376164, Length=278, Percent_Identity=27.6978417266187, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI218777837, Length=232, Percent_Identity=25, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17562298, Length=250, Percent_Identity=25.2, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17554012, Length=239, Percent_Identity=23.8493723849372, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.3 [H]

Molecular weight: Translated: 35625; Mature: 35625

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAWAVPPRADTLASYNAVTPDHKRDVMAFENFTPFRVAVGDVDIFGVKGGAGPPLLLLH
CCCCCCCCCCHHHHCCCCCCCCCCCCHHHHCCCCCEEEEECCEEEEEECCCCCCCEEEEE
GHPQSHLIWRKCAARLAKHFTVIATDLRGYGASGKPPSDAEHTPYSKRVMAADQVAVMRH
CCCCHHHHHHHHHHHHHHHHEEEEEHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
FGFERFLVCAHDRGARVAHRMALDHPDAVERLMLLDIAPTLAMYEATDRTFATHYFHWFF
CCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEE
LIQPEPLPETLIGANPAAYVDAVMGGRHAGLAPFDPAALDAYRSALAQPGAVHAMCEDYR
EECCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHC
ASASIDLEHDRADIERGHKIGCPLRVLWGDKGVIEKCFDALAEWRHVARDVSGRALPCGH
CCCEEEECCCHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YIPEEASDELVAEMLSFFEAVEQ
CCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLAWAVPPRADTLASYNAVTPDHKRDVMAFENFTPFRVAVGDVDIFGVKGGAGPPLLLLH
CCCCCCCCCCHHHHCCCCCCCCCCCCHHHHCCCCCEEEEECCEEEEEECCCCCCCEEEEE
GHPQSHLIWRKCAARLAKHFTVIATDLRGYGASGKPPSDAEHTPYSKRVMAADQVAVMRH
CCCCHHHHHHHHHHHHHHHHEEEEEHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
FGFERFLVCAHDRGARVAHRMALDHPDAVERLMLLDIAPTLAMYEATDRTFATHYFHWFF
CCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEE
LIQPEPLPETLIGANPAAYVDAVMGGRHAGLAPFDPAALDAYRSALAQPGAVHAMCEDYR
EECCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHC
ASASIDLEHDRADIERGHKIGCPLRVLWGDKGVIEKCFDALAEWRHVARDVSGRALPCGH
CCCEEEECCCHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YIPEEASDELVAEMLSFFEAVEQ
CCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA