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The map label for this gene is gacA [H]

Identifier: 91781499

GI number: 91781499

Start: 139700

End: 140356

Strand: Reverse

Name: gacA [H]

Synonym: Bxe_A4347

Alternate gene names: 91781499

Gene position: 140356-139700 (Counterclockwise)

Preceding gene: 91781500

Following gene: 91781498

Centisome position: 2.87

GC content: 63.77

Gene sequence:

>657_bases
ATGAGCCTGCGCATTCTGCTCGTCGATGATCACGCAGTGGTTCGCCAGGGCGTCCGTCAGTTGCTGCTCGACCGCGGCGT
GGCGCGCGAAGTGACCGAAGCGCAAAGCGGTGCCGAAGCGCTCGACGCCGTCGCCCGGTTCAGCTACGACGTGGTGCTGC
TGGACATTTCGCTGCCCGACATGAACGGCGTCGAAGTACTCAAGCGTCTGAAGCGCAAGGCGCCGCGCGTGGCCGTGCTG
ATGTTCTCGATGTACCGCGAGGACCAGTACGCCGTGCGCGCGCTGAAAGCGGGCGCCGCCGGCTACCTGTCGAAAACCGT
CGACGCCACGCAGATGATCGGCGCGCTCCAGCAGGTCGCGGCAGGCCGCAAATACGTGAGCCCGGCGATGGCCGAGGCGC
TCGCGGACTACGTTTCCTTCGACGGCGAACAGATGCCGCACGAAAAGCTCTCCGACCGCGAATATCAGACGCTGTGCATG
CTCGCGTCGGGCAAGCGGCTGACGGATATTGCCGTGGCGCTCTCGCTGTCGGTGAAAACGGTCAGCGTTTATCGCACCCG
TCTGCTCGAAAAGATGAAGCTGCGCAACAACGCCGAACTGACGTTCTACGTGATGAGCAACCGGCTCATCGATCTGAATC
CCGCGATGGCGGGCTGA

Upstream 100 bases:

>100_bases
TCGCGCGGAACCATCGTTCGCGCGCGCTTTGCCTGGGACGCCATGCTGGCGCACGCGCCGCGCGCCCGTCGGCACGCCCT
GCAATCGTGAGCGTGACGTC

Downstream 100 bases:

>100_bases
CCGCTCTCGCCGCCGACGCCTGCCGACGCTGCTCTGTGTGTGCTGAAAAGCTCGCGCCCGGCACGGCCGGTTTGCAGGAG
AGCAATTCCCTCCCGACTGC

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSLRILLVDDHAVVRQGVRQLLLDRGVAREVTEAQSGAEALDAVARFSYDVVLLDISLPDMNGVEVLKRLKRKAPRVAVL
MFSMYREDQYAVRALKAGAAGYLSKTVDATQMIGALQQVAAGRKYVSPAMAEALADYVSFDGEQMPHEKLSDREYQTLCM
LASGKRLTDIAVALSLSVKTVSVYRTRLLEKMKLRNNAELTFYVMSNRLIDLNPAMAG

Sequences:

>Translated_218_residues
MSLRILLVDDHAVVRQGVRQLLLDRGVAREVTEAQSGAEALDAVARFSYDVVLLDISLPDMNGVEVLKRLKRKAPRVAVL
MFSMYREDQYAVRALKAGAAGYLSKTVDATQMIGALQQVAAGRKYVSPAMAEALADYVSFDGEQMPHEKLSDREYQTLCM
LASGKRLTDIAVALSLSVKTVSVYRTRLLEKMKLRNNAELTFYVMSNRLIDLNPAMAG
>Mature_217_residues
SLRILLVDDHAVVRQGVRQLLLDRGVAREVTEAQSGAEALDAVARFSYDVVLLDISLPDMNGVEVLKRLKRKAPRVAVLM
FSMYREDQYAVRALKAGAAGYLSKTVDATQMIGALQQVAAGRKYVSPAMAEALADYVSFDGEQMPHEKLSDREYQTLCML
ASGKRLTDIAVALSLSVKTVSVYRTRLLEKMKLRNNAELTFYVMSNRLIDLNPAMAG

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=32.3809523809524, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=37.8787878787879, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788521, Length=209, Percent_Identity=34.9282296650718, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1788712, Length=206, Percent_Identity=27.1844660194175, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1786747, Length=200, Percent_Identity=30, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787473, Length=212, Percent_Identity=33.9622641509434, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1788546, Length=217, Percent_Identity=24.4239631336406, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=35.7798165137615, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790437, Length=123, Percent_Identity=34.9593495934959, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=32.7731092436975, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790299, Length=109, Percent_Identity=32.1100917431193, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24131; Mature: 24000

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRILLVDDHAVVRQGVRQLLLDRGVAREVTEAQSGAEALDAVARFSYDVVLLDISLPD
CCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCEEEEEEEECCC
MNGVEVLKRLKRKAPRVAVLMFSMYREDQYAVRALKAGAAGYLSKTVDATQMIGALQQVA
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
AGRKYVSPAMAEALADYVSFDGEQMPHEKLSDREYQTLCMLASGKRLTDIAVALSLSVKT
CCCHHCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
VSVYRTRLLEKMKLRNNAELTFYVMSNRLIDLNPAMAG
HHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCCCCC
>Mature Secondary Structure 
SLRILLVDDHAVVRQGVRQLLLDRGVAREVTEAQSGAEALDAVARFSYDVVLLDISLPD
CEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCEEEEEEEECCC
MNGVEVLKRLKRKAPRVAVLMFSMYREDQYAVRALKAGAAGYLSKTVDATQMIGALQQVA
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
AGRKYVSPAMAEALADYVSFDGEQMPHEKLSDREYQTLCMLASGKRLTDIAVALSLSVKT
CCCHHCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
VSVYRTRLLEKMKLRNNAELTFYVMSNRLIDLNPAMAG
HHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]