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The map label for this gene is rlmI [H]

Identifier: 91781470

GI number: 91781470

Start: 103667

End: 104869

Strand: Reverse

Name: rlmI [H]

Synonym: Bxe_A4376

Alternate gene names: 91781470

Gene position: 104869-103667 (Counterclockwise)

Preceding gene: 91781471

Following gene: 91781469

Centisome position: 2.14

GC content: 64.92

Gene sequence:

>1203_bases
ATGAATACCGTTACGCTCAAACCGTCGAAAGAAAAATCGCTGCTGCGCCGCCATCCGTGGGTCTACGCCAACGCGATCGA
CCGGATCGACGGCAATCCCGCGCCCGGCGCCACCGTGCTGGTACGCGCACACGATGGCCGCTTTCTCGCGCGCGCGGCCT
ACAGTCCGCATTCGCAGATCCGCGCCCGCGTGTGGAGCTTCGACGAGACCGAGCCGATCGACCACGCGTTCTTCAAACGC
CGCGTGCAGCGCGCGCTCGCGCACCGTCAGACGATGGTTCACGACACCGGCGCGGTGCGGCTGATTTTCGGCGAGGCGGA
CGGCCTGCCCGGCCTGATCGTCGATCACTACGTGGCCGAAGACGAAGGCCAGCGCAGCCAGCTGGTCTGCCAGTTCATGG
CCGCGGGCGTCGAGGCGTGGAAAGAGGCGATCGTCGCGGCGCTGACGGGCGCGACCGGCTGCCCGAATGTGTATGAACGC
TCGGACGTGTCGATTCGCCAGAAGGAAGGGCTCGAACAGATCACCGGCGTGCTGGCGGGCGAACCGCCGTCGGAAGCGCT
GATCGTGAGCGAGAACGACGTGCGGTATCACGTCGATGTGCGCAACGGCCACAAAACCGGCTTTTACGTCGATCAGCGCG
ACAACCGCCCGCTGGTGCAGCAGCTCGCGCAGGATCGCGATATGTTGAACTGCTTCTGCTACACCGGCGGCTTCTCACTG
GCCGCGCTGAAAGGCGGCGCCAAACGGGTGGTGTCGATCGATTCGTCGGGCGACGCGCTGGCGATCGCGCAGCAGAACGT
GGCGGCCAACGGTTTCGACGCCGAACGCGCCACGTGGCTCGACGCCGATGCCTTCAAAACGCTGCGCCGTCTTTACGACG
ACGGCGAGCGCTTCGATCTGGTCGTGCTCGATCCGCCGAAATTCGCGCCGTCGCGTGAACATGTGGACCGCGCGTCGCGC
GCTTATAAGGACATCAATCTGACCGGCCTGAAATTGCTGCGGCCGGGCGGCCTGCTGTTCACCTATTCGTGCTCCGGCGC
GATCGACTCCGAACTGTTTCAGAAGATCATCTCGGGCGCGGCCGCCGACGCGCGCGTGGATGCCCGGATTCTCAAGCGGC
TTGGCGCCGGGATGGATCATCCCTTGCTGACGGCATTTCCGGAAGGCGAGTATCTGAAGGGCCTGCTGTTGCAAATCGCC
TGA

Upstream 100 bases:

>100_bases
ACTGACGCCGCGACGCGAGTCGCGGCGTACGGTCTCCGCCTTCGCTTTACCTGCCGCGCGCGCCGAGCCGCGCGGCCCAT
TGCTCACTCTGACCACCCTG

Downstream 100 bases:

>100_bases
TCGCCCATAATTTGAGGGGTATTTCCCGGCGCCGCCGGCCCGGGCGAAAGCGGGGCCCGTATCCGCGCGCCAGCGCCGGC
CCGGCGAATATGTTTCAATA

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MNTVTLKPSKEKSLLRRHPWVYANAIDRIDGNPAPGATVLVRAHDGRFLARAAYSPHSQIRARVWSFDETEPIDHAFFKR
RVQRALAHRQTMVHDTGAVRLIFGEADGLPGLIVDHYVAEDEGQRSQLVCQFMAAGVEAWKEAIVAALTGATGCPNVYER
SDVSIRQKEGLEQITGVLAGEPPSEALIVSENDVRYHVDVRNGHKTGFYVDQRDNRPLVQQLAQDRDMLNCFCYTGGFSL
AALKGGAKRVVSIDSSGDALAIAQQNVAANGFDAERATWLDADAFKTLRRLYDDGERFDLVVLDPPKFAPSREHVDRASR
AYKDINLTGLKLLRPGGLLFTYSCSGAIDSELFQKIISGAAADARVDARILKRLGAGMDHPLLTAFPEGEYLKGLLLQIA

Sequences:

>Translated_400_residues
MNTVTLKPSKEKSLLRRHPWVYANAIDRIDGNPAPGATVLVRAHDGRFLARAAYSPHSQIRARVWSFDETEPIDHAFFKR
RVQRALAHRQTMVHDTGAVRLIFGEADGLPGLIVDHYVAEDEGQRSQLVCQFMAAGVEAWKEAIVAALTGATGCPNVYER
SDVSIRQKEGLEQITGVLAGEPPSEALIVSENDVRYHVDVRNGHKTGFYVDQRDNRPLVQQLAQDRDMLNCFCYTGGFSL
AALKGGAKRVVSIDSSGDALAIAQQNVAANGFDAERATWLDADAFKTLRRLYDDGERFDLVVLDPPKFAPSREHVDRASR
AYKDINLTGLKLLRPGGLLFTYSCSGAIDSELFQKIISGAAADARVDARILKRLGAGMDHPLLTAFPEGEYLKGLLLQIA
>Mature_400_residues
MNTVTLKPSKEKSLLRRHPWVYANAIDRIDGNPAPGATVLVRAHDGRFLARAAYSPHSQIRARVWSFDETEPIDHAFFKR
RVQRALAHRQTMVHDTGAVRLIFGEADGLPGLIVDHYVAEDEGQRSQLVCQFMAAGVEAWKEAIVAALTGATGCPNVYER
SDVSIRQKEGLEQITGVLAGEPPSEALIVSENDVRYHVDVRNGHKTGFYVDQRDNRPLVQQLAQDRDMLNCFCYTGGFSL
AALKGGAKRVVSIDSSGDALAIAQQNVAANGFDAERATWLDADAFKTLRRLYDDGERFDLVVLDPPKFAPSREHVDRASR
AYKDINLTGLKLLRPGGLLFTYSCSGAIDSELFQKIISGAAADARVDARILKRLGAGMDHPLLTAFPEGEYLKGLLLQIA

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=398, Percent_Identity=45.2261306532663, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1787181, Length=165, Percent_Identity=30.9090909090909, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44010; Mature: 44010

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVTLKPSKEKSLLRRHPWVYANAIDRIDGNPAPGATVLVRAHDGRFLARAAYSPHSQI
CCCEECCCCHHHHHHHHCCCEEECHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCHHHH
RARVWSFDETEPIDHAFFKRRVQRALAHRQTMVHDTGAVRLIFGEADGLPGLIVDHYVAE
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCHHHHHHHCC
DEGQRSQLVCQFMAAGVEAWKEAIVAALTGATGCPNVYERSDVSIRQKEGLEQITGVLAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCC
EPPSEALIVSENDVRYHVDVRNGHKTGFYVDQRDNRPLVQQLAQDRDMLNCFCYTGGFSL
CCCCCEEEEECCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHCCCHHEEEEEEECCCEE
AALKGGAKRVVSIDSSGDALAIAQQNVAANGFDAERATWLDADAFKTLRRLYDDGERFDL
EEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCEECCHHHHHHHHHHHCCCCEEEE
VVLDPPKFAPSREHVDRASRAYKDINLTGLKLLRPGGLLFTYSCSGAIDSELFQKIISGA
EEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHCCC
AADARVDARILKRLGAGMDHPLLTAFPEGEYLKGLLLQIA
CCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MNTVTLKPSKEKSLLRRHPWVYANAIDRIDGNPAPGATVLVRAHDGRFLARAAYSPHSQI
CCCEECCCCHHHHHHHHCCCEEECHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCHHHH
RARVWSFDETEPIDHAFFKRRVQRALAHRQTMVHDTGAVRLIFGEADGLPGLIVDHYVAE
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCHHHHHHHCC
DEGQRSQLVCQFMAAGVEAWKEAIVAALTGATGCPNVYERSDVSIRQKEGLEQITGVLAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCC
EPPSEALIVSENDVRYHVDVRNGHKTGFYVDQRDNRPLVQQLAQDRDMLNCFCYTGGFSL
CCCCCEEEEECCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHCCCHHEEEEEEECCCEE
AALKGGAKRVVSIDSSGDALAIAQQNVAANGFDAERATWLDADAFKTLRRLYDDGERFDL
EEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCEECCHHHHHHHHHHHCCCCEEEE
VVLDPPKFAPSREHVDRASRAYKDINLTGLKLLRPGGLLFTYSCSGAIDSELFQKIISGA
EEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHCCC
AADARVDARILKRLGAGMDHPLLTAFPEGEYLKGLLLQIA
CCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA