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The map label for this gene is pckG [H]

Identifier: 91781424

GI number: 91781424

Start: 53941

End: 55797

Strand: Direct

Name: pckG [H]

Synonym: Bxe_A4422

Alternate gene names: 91781424

Gene position: 53941-55797 (Clockwise)

Preceding gene: 91781422

Following gene: 91781426

Centisome position: 1.1

GC content: 64.4

Gene sequence:

>1857_bases
ATGAACCATCCCTCATTCGAAGGGCAGCCCACTATCGAGGCGCCCGCGTGGGTCAAAAACCGAAAACTGGTCGACTGGGT
GCAGCGCGTCGCCGCCATGACCAAGCCGGACAGAATCGTCTGGTGCGACGGCTCACAGGAGGAATACGACCGGCTTTGCG
CGCAGATGGTCGAGGCGGGCACCCTCAAAAAACTCAACCCCGCGAAGCGTCCCAACTCCTATCTGGCATGGTCTGACCCG
TCCGACGTGGCGCGCGTCGAAGATCGCACCTTCATCTGTTCGCAGCAGCGAGAGGACGCGGGCCCCACCAACAACTGGGT
CGCCCCGGCCGAGATGCGCTCGACGCTCGGCGGCCTGTTCGAAGGCGCCATGCGCGGCCGCACCATGTATGTGGTGCCGT
TTTCGATGGGGCCACTCGGTTCGCCGATTGCGCACATCGGCGTGGAACTGAGCGACAGCCCGTACGTGGTCACCAACATG
CGCATCATGACGCGCATGGGCCGCAAGGTGTACGACGTGCTGGGCGAAGACGGCGAGTTCGTGCCGTGCGTCCACTCCGT
GGGCGCACCGCTCGCGGCGGGCGAAAAAGACGTGCCGTGGCCGTGCAACGCAACCAAATACATCGTCCACTTTCCGGAAT
CGCGCGAAATCTGGAGCTATGGCTCGGGCTACGGCGGCAATGCGCTACTCGGCAAGAAGTGTTTCGCGCTGCGCATCGCC
TCGACTATGGGCCGCGACGAAGGCTGGCTGGCCGAGCACATGCTGATTCTCGGCGTGACCTCGCCGGAAGGCCGCAAGCA
TCATGTGGCGGCGGCGTTTCCGTCGGCGTGCGGCAAGACCAACTTTGCGATGCTGATTCCGCCGGAAGGCCTGAACGGCT
GGAAAATCTCCACGATCGGCGACGATATCGCGTGGATCAAACCGGGCAAGGACGGCCGCCTGTATGCGATCAATCCGGAA
GCCGGCTACTTCGGCGTGGCGCCGGGCACGAGCGAAAAGACCAACTTCAACGCAATGGCCACGTTGAAGGAAAACGTGAT
CTTCACGAACGTCGCGCTGACCGACGACGGCGACGTCTGGTGGGAAGGCATGACCGACGAACCGCCGGCGCACCTGATCG
ACTGGCAGGGCAAGGACTGGACGCCGGCCGGCGCGAAGCAAAGCGGCCGCAAGGCGGCGCACCCGAACGCGCGCTTTACG
GCGCCGGCCTCGCAATGCCCGTCTATCGACGCCGACTGGGAAAACCCGGCGGGCGTCGCGATCGACGCGTTCATCTTCGG
CGGACGCCGTTCCACCACCGTGCCGCTCGTCACCGAAGCGCGCAACTGGGTGGAAGGCGTCTACATGGCGGCGACTATGG
GCTCGGAGACCACCGCCGCCGCCGCCGGCCAGCAGGGCGTGGTGCGCCGCGACCCGTTCGCGATGCTGCCGTTCTGCGGC
TACAACATGAGCGACTACTTCGGTCACTGGCTGAAAACGGGCGAGCGTCTGGAGCAACTCGGCGCGAAGCTGCCGAAAAT
CTTCTGCGTCAACTGGTTTCGCAAGGGCGCGGACGGCAAGTTCGTCTGGCCGGGCTTCGGCGAGAACATGCGGGTGCTGA
GCTGGATGGTCGGCCGGGTCGAAGGCTCTGCTCAAGGTGCGGAACATGCATTCGGCGTGTCGCCTCACTATGAGGACATC
GACTGGAGCGGGCTGAATTTCAGCCGCGAGCAGTTTGAGCAGGTGATTTCCGTGGATGACGCCGCCTGGCGCGACGAACT
CGCCCTGCACGCCGAGCTGTTTGACACATTGCAACACGGCTTGCCGCCGGCGCTAACCCAGGCCAAACGCACACTCGAAG
GAAAACTCGCCGCCTGA

Upstream 100 bases:

>100_bases
GGGTGGCAGGAACCCCGGCGTGAAGAATTGAACGCCTCTCGCATGCAGCGTCGCTGGGCTTGTACCGTTTTTTTATTGCT
TTCAAAACGGAGACAAGTCG

Downstream 100 bases:

>100_bases
TCAGGTGAGTATTCACTTCGCATGAAGAACCGCCTTCGGGCGGTTTTCTTTTGGTGCCGACAGCTTGGCGATTTTTTCTG
ATTCCGCAACGCCAACGCGA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MNHPSFEGQPTIEAPAWVKNRKLVDWVQRVAAMTKPDRIVWCDGSQEEYDRLCAQMVEAGTLKKLNPAKRPNSYLAWSDP
SDVARVEDRTFICSQQREDAGPTNNWVAPAEMRSTLGGLFEGAMRGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVVTNM
RIMTRMGRKVYDVLGEDGEFVPCVHSVGAPLAAGEKDVPWPCNATKYIVHFPESREIWSYGSGYGGNALLGKKCFALRIA
STMGRDEGWLAEHMLILGVTSPEGRKHHVAAAFPSACGKTNFAMLIPPEGLNGWKISTIGDDIAWIKPGKDGRLYAINPE
AGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTDEPPAHLIDWQGKDWTPAGAKQSGRKAAHPNARFT
APASQCPSIDADWENPAGVAIDAFIFGGRRSTTVPLVTEARNWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
YNMSDYFGHWLKTGERLEQLGAKLPKIFCVNWFRKGADGKFVWPGFGENMRVLSWMVGRVEGSAQGAEHAFGVSPHYEDI
DWSGLNFSREQFEQVISVDDAAWRDELALHAELFDTLQHGLPPALTQAKRTLEGKLAA

Sequences:

>Translated_618_residues
MNHPSFEGQPTIEAPAWVKNRKLVDWVQRVAAMTKPDRIVWCDGSQEEYDRLCAQMVEAGTLKKLNPAKRPNSYLAWSDP
SDVARVEDRTFICSQQREDAGPTNNWVAPAEMRSTLGGLFEGAMRGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVVTNM
RIMTRMGRKVYDVLGEDGEFVPCVHSVGAPLAAGEKDVPWPCNATKYIVHFPESREIWSYGSGYGGNALLGKKCFALRIA
STMGRDEGWLAEHMLILGVTSPEGRKHHVAAAFPSACGKTNFAMLIPPEGLNGWKISTIGDDIAWIKPGKDGRLYAINPE
AGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTDEPPAHLIDWQGKDWTPAGAKQSGRKAAHPNARFT
APASQCPSIDADWENPAGVAIDAFIFGGRRSTTVPLVTEARNWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
YNMSDYFGHWLKTGERLEQLGAKLPKIFCVNWFRKGADGKFVWPGFGENMRVLSWMVGRVEGSAQGAEHAFGVSPHYEDI
DWSGLNFSREQFEQVISVDDAAWRDELALHAELFDTLQHGLPPALTQAKRTLEGKLAA
>Mature_618_residues
MNHPSFEGQPTIEAPAWVKNRKLVDWVQRVAAMTKPDRIVWCDGSQEEYDRLCAQMVEAGTLKKLNPAKRPNSYLAWSDP
SDVARVEDRTFICSQQREDAGPTNNWVAPAEMRSTLGGLFEGAMRGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVVTNM
RIMTRMGRKVYDVLGEDGEFVPCVHSVGAPLAAGEKDVPWPCNATKYIVHFPESREIWSYGSGYGGNALLGKKCFALRIA
STMGRDEGWLAEHMLILGVTSPEGRKHHVAAAFPSACGKTNFAMLIPPEGLNGWKISTIGDDIAWIKPGKDGRLYAINPE
AGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTDEPPAHLIDWQGKDWTPAGAKQSGRKAAHPNARFT
APASQCPSIDADWENPAGVAIDAFIFGGRRSTTVPLVTEARNWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
YNMSDYFGHWLKTGERLEQLGAKLPKIFCVNWFRKGADGKFVWPGFGENMRVLSWMVGRVEGSAQGAEHAFGVSPHYEDI
DWSGLNFSREQFEQVISVDDAAWRDELALHAELFDTLQHGLPPALTQAKRTLEGKLAA

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=607, Percent_Identity=50.2471169686985, Blast_Score=586, Evalue=1e-167,
Organism=Homo sapiens, GI66346721, Length=606, Percent_Identity=50.4950495049505, Blast_Score=570, Evalue=1e-162,
Organism=Homo sapiens, GI66346723, Length=371, Percent_Identity=54.4474393530997, Blast_Score=390, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17555492, Length=645, Percent_Identity=48.062015503876, Blast_Score=554, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI71989645, Length=580, Percent_Identity=49.1379310344828, Blast_Score=550, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI71989658, Length=580, Percent_Identity=49.1379310344828, Blast_Score=548, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI32564411, Length=579, Percent_Identity=50.9499136442142, Blast_Score=545, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71989653, Length=576, Percent_Identity=49.3055555555556, Blast_Score=545, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI32564407, Length=531, Percent_Identity=51.4124293785311, Blast_Score=504, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71989649, Length=491, Percent_Identity=51.7311608961303, Blast_Score=489, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI32564409, Length=377, Percent_Identity=48.2758620689655, Blast_Score=321, Evalue=7e-88,
Organism=Caenorhabditis elegans, GI17540956, Length=523, Percent_Identity=25.8126195028681, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24655085, Length=605, Percent_Identity=50.2479338842975, Blast_Score=579, Evalue=1e-165,
Organism=Drosophila melanogaster, GI24655082, Length=607, Percent_Identity=48.9291598023064, Blast_Score=566, Evalue=1e-161,
Organism=Drosophila melanogaster, GI161077226, Length=576, Percent_Identity=50.1736111111111, Blast_Score=546, Evalue=1e-155,
Organism=Drosophila melanogaster, GI24655088, Length=510, Percent_Identity=52.156862745098, Blast_Score=507, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 67868; Mature: 67868

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00505 PEPCK_GTP ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHPSFEGQPTIEAPAWVKNRKLVDWVQRVAAMTKPDRIVWCDGSQEEYDRLCAQMVEAG
CCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCC
TLKKLNPAKRPNSYLAWSDPSDVARVEDRTFICSQQREDAGPTNNWVAPAEMRSTLGGLF
CCCCCCCCCCCCCEEEECCCCHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
EGAMRGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVVTNMRIMTRMGRKVYDVLGEDGEF
HHHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCE
VPCVHSVGAPLAAGEKDVPWPCNATKYIVHFPESREIWSYGSGYGGNALLGKKCFALRIA
EHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCHHCCHHHHHHHH
STMGRDEGWLAEHMLILGVTSPEGRKHHVAAAFPSACGKTNFAMLIPPEGLNGWKISTIG
HHCCCCCCHHEEEEEEEEECCCCCCCEEEEEECHHHCCCCCEEEEECCCCCCCEEEEECC
DDIAWIKPGKDGRLYAINPEAGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVW
CCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEEEECCCCEE
WEGMTDEPPAHLIDWQGKDWTPAGAKQSGRKAAHPNARFTAPASQCPSIDADWENPAGVA
ECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCEE
IDAFIFGGRRSTTVPLVTEARNWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
EEEEEECCCCCCCCEEHHHHHHHHHHEEEEEECCCCHHHHHCCCCCCEECCCCEECCCCC
YNMSDYFGHWLKTGERLEQLGAKLPKIFCVNWFRKGADGKFVWPGFGENMRVLSWMVGRV
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCEECCCCCCCHHHHHHHHHHC
EGSAQGAEHAFGVSPHYEDIDWSGLNFSREQFEQVISVDDAAWRDELALHAELFDTLQHG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
LPPALTQAKRTLEGKLAA
CCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNHPSFEGQPTIEAPAWVKNRKLVDWVQRVAAMTKPDRIVWCDGSQEEYDRLCAQMVEAG
CCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCC
TLKKLNPAKRPNSYLAWSDPSDVARVEDRTFICSQQREDAGPTNNWVAPAEMRSTLGGLF
CCCCCCCCCCCCCEEEECCCCHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
EGAMRGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVVTNMRIMTRMGRKVYDVLGEDGEF
HHHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCE
VPCVHSVGAPLAAGEKDVPWPCNATKYIVHFPESREIWSYGSGYGGNALLGKKCFALRIA
EHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCHHCCHHHHHHHH
STMGRDEGWLAEHMLILGVTSPEGRKHHVAAAFPSACGKTNFAMLIPPEGLNGWKISTIG
HHCCCCCCHHEEEEEEEEECCCCCCCEEEEEECHHHCCCCCEEEEECCCCCCCEEEEECC
DDIAWIKPGKDGRLYAINPEAGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVW
CCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEEEECCCCEE
WEGMTDEPPAHLIDWQGKDWTPAGAKQSGRKAAHPNARFTAPASQCPSIDADWENPAGVA
ECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCEE
IDAFIFGGRRSTTVPLVTEARNWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
EEEEEECCCCCCCCEEHHHHHHHHHHEEEEEECCCCHHHHHCCCCCCEECCCCEECCCCC
YNMSDYFGHWLKTGERLEQLGAKLPKIFCVNWFRKGADGKFVWPGFGENMRVLSWMVGRV
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCEECCCCCCCHHHHHHHHHHC
EGSAQGAEHAFGVSPHYEDIDWSGLNFSREQFEQVISVDDAAWRDELALHAELFDTLQHG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
LPPALTQAKRTLEGKLAA
CCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA