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The map label for this gene is hisJ [H]

Identifier: 91781413

GI number: 91781413

Start: 36117

End: 36911

Strand: Direct

Name: hisJ [H]

Synonym: Bxe_A4434

Alternate gene names: 91781413

Gene position: 36117-36911 (Clockwise)

Preceding gene: 91781412

Following gene: 91781415

Centisome position: 0.74

GC content: 62.14

Gene sequence:

>795_bases
ATGAAATTGCTCAAGCCTCTATTGGCACTGGCCTGCGCATTTGCGTCGCTCGCGGCTGCATCTGCCGTGAGCGCCGCCGA
CACCTCGACGCTGCGTTTCGGCCTCGAAGCGCAGTATCCGCCGTTCGAATCGAAGGGGCCGAACGGGGAGTTGCAGGGTC
TGGATATCGACGTCGGCAATGCGGTCTGCGCCGCTGCGCATATGACCTGCAAATGGGTCGAGACTTCGTTCGACGGGCTG
ATTCCCGCGCTGCAGGGCCGCAAGTTCGACGCGATCAATTCGGCGATGAATGCGACCGAACAACGCCGGCAGGCGATTGA
TTTCACCACCGTCGTGTACCGCGTGCCGACGCAGCTGATCGCAAAGCGCGACAGCGGTCTGCTGCCGACAACAGCCTCGC
TGAAGGGCAAACGCGTGGGCGTGTTGCAGTCGTCGATTCAGGAAACCTTCGCCAAGGCGCATTGGGAATCGGCGGGCGTG
ACTGTCGTGCCGTATCAGGACCAGAACCAGGTTTATACGGATCTCGTGGCGGGCCGTCTCGACGCGACGCTCGTGCTGGC
GCCGGCCGGACAGACAGGTTTTCTGTCGAAGCCGAACGGCGCCGGTTACGCCTTCGTCGGCGAGCCGGTGCGCGACGACA
GGATTCTCGGTAGCGGCATTGCATACGGGATTCGCAAGGGCGATACGGCGTTGCGTGATCGCCTGAATGCGGCGATCGCA
AAGGTGCAGGCCGATGGTACGGTGAAGACGCTGGCCACGAAATATCTCGGCAATATCGACGTGACGGCGAAGTGA

Upstream 100 bases:

>100_bases
GCGATGGCTCTATTTTTGACCGCAGTATGATCGTCGCGCCGTCACGCATCAGCGCGCCGCAACACGGCGTGACAATAAAT
CGAAGGGAAAGGGACCTGCT

Downstream 100 bases:

>100_bases
TCCGGGGCGCGTCTGGATGCGCCGCGTTGATCGTGTTGCGGATGATGGGCTGTTGTTCCTGAGTCGGCTGGCTGACGCAG
TGCGTTTGCCGGCTACGGAT

Product: ABC amino acid transporter, periplasmic ligand binding protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKLLKPLLALACAFASLAAASAVSAADTSTLRFGLEAQYPPFESKGPNGELQGLDIDVGNAVCAAAHMTCKWVETSFDGL
IPALQGRKFDAINSAMNATEQRRQAIDFTTVVYRVPTQLIAKRDSGLLPTTASLKGKRVGVLQSSIQETFAKAHWESAGV
TVVPYQDQNQVYTDLVAGRLDATLVLAPAGQTGFLSKPNGAGYAFVGEPVRDDRILGSGIAYGIRKGDTALRDRLNAAIA
KVQADGTVKTLATKYLGNIDVTAK

Sequences:

>Translated_264_residues
MKLLKPLLALACAFASLAAASAVSAADTSTLRFGLEAQYPPFESKGPNGELQGLDIDVGNAVCAAAHMTCKWVETSFDGL
IPALQGRKFDAINSAMNATEQRRQAIDFTTVVYRVPTQLIAKRDSGLLPTTASLKGKRVGVLQSSIQETFAKAHWESAGV
TVVPYQDQNQVYTDLVAGRLDATLVLAPAGQTGFLSKPNGAGYAFVGEPVRDDRILGSGIAYGIRKGDTALRDRLNAAIA
KVQADGTVKTLATKYLGNIDVTAK
>Mature_264_residues
MKLLKPLLALACAFASLAAASAVSAADTSTLRFGLEAQYPPFESKGPNGELQGLDIDVGNAVCAAAHMTCKWVETSFDGL
IPALQGRKFDAINSAMNATEQRRQAIDFTTVVYRVPTQLIAKRDSGLLPTTASLKGKRVGVLQSSIQETFAKAHWESAGV
TVVPYQDQNQVYTDLVAGRLDATLVLAPAGQTGFLSKPNGAGYAFVGEPVRDDRILGSGIAYGIRKGDTALRDRLNAAIA
KVQADGTVKTLATKYLGNIDVTAK

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=44.8275862068966, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1788649, Length=254, Percent_Identity=42.9133858267717, Blast_Score=213, Evalue=8e-57,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=33.469387755102, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787085, Length=244, Percent_Identity=32.3770491803279, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI1788228, Length=261, Percent_Identity=31.8007662835249, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787031, Length=255, Percent_Identity=27.4509803921569, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27917; Mature: 27917

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLKPLLALACAFASLAAASAVSAADTSTLRFGLEAQYPPFESKGPNGELQGLDIDVGN
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEECCC
AVCAAAHMTCKWVETSFDGLIPALQGRKFDAINSAMNATEQRRQAIDFTTVVYRVPTQLI
HHHHHHHHHHEEHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKRDSGLLPTTASLKGKRVGVLQSSIQETFAKAHWESAGVTVVPYQDQNQVYTDLVAGRL
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCC
DATLVLAPAGQTGFLSKPNGAGYAFVGEPVRDDRILGSGIAYGIRKGDTALRDRLNAAIA
CEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEECCCHHHCCCCCCHHHHHHHHHHHH
KVQADGTVKTLATKYLGNIDVTAK
HHCCCCHHHHHHHHHHCCEEEEEC
>Mature Secondary Structure
MKLLKPLLALACAFASLAAASAVSAADTSTLRFGLEAQYPPFESKGPNGELQGLDIDVGN
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEECCC
AVCAAAHMTCKWVETSFDGLIPALQGRKFDAINSAMNATEQRRQAIDFTTVVYRVPTQLI
HHHHHHHHHHEEHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKRDSGLLPTTASLKGKRVGVLQSSIQETFAKAHWESAGVTVVPYQDQNQVYTDLVAGRL
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCC
DATLVLAPAGQTGFLSKPNGAGYAFVGEPVRDDRILGSGIAYGIRKGDTALRDRLNAAIA
CEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEECCCHHHCCCCCCHHHHHHHHHHHH
KVQADGTVKTLATKYLGNIDVTAK
HHCCCCHHHHHHHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]