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The map label for this gene is dnaN [H]

Identifier: 91781386

GI number: 91781386

Start: 2047

End: 3150

Strand: Direct

Name: dnaN [H]

Synonym: Bxe_A4461

Alternate gene names: 91781386

Gene position: 2047-3150 (Clockwise)

Preceding gene: 91781385

Following gene: 91781387

Centisome position: 0.04

GC content: 59.6

Gene sequence:

>1104_bases
ATGCAACTGGTCAAGACCGAACGCGATAACCTCCTCAGGCCGCTGCAAACTGTGAGCGGCATCGTCGAACGCCGCCATAC
GTTGCCGATCCTCGCCAATTTGCTGATTACCAAGAACGGCCCGGACGTGTCGTTCCTGTCCACCGACCTCGAGTTGCAGA
TCACCACGCGTGCCGATTTCGGCGTGGGCGGCGATTCGGTGGCGACCACGGTGGCAGCGAGAAAGCTCCTCGACATTCTG
CGCGCCATGCCCGACGGCCAGGTCACGCTCACGCTGAACGACAAGCGTCTGACCGTGCAATCCGGCAAGAGCCGCTTTGC
GCTGCAAACGCTCGCCGCGGACGAATTCCCGACGGTCGCGCAAGCTAAAGACTACGGCGCGAACCTCGTGGTTCCCCAGA
AGACGTTCCGCCAGTTGCTCGGCATGGTCCATTTTTCGATGGCCCAGCAGGACATCCGCTACTACCTGAACGGCATGCTG
CTGGTAGTGGACGGCGACCAGCTGATGGCGGTCGCGACCGACGGACACCGTCTGGCGTTCTCGTCCATGAAGATCGAAGG
TTCGTTCCAGCGTCAGGAAGTGATCATTCCGCGCAAGACGATTCTGGAACTCCAGCGTCTGCTGGAAGACATCGACGACA
CGCTGAAGATCGACATCGCGCAGACGCAGGTCAAGTTCACGTTCGGCCAGGTCGAACTGGTGTCGAAGCTGGTGGAAGGC
AAATTCCCCGACTTCCAGCGCGTGATTCCGAAGTCGCACAAGAATCAGTTCGTGATCGGCCGTGAAGAACTGCAGCGCTC
GCTGCAACGCGCCGCGATTCTGACCTCGGACAAATTCAAGGGCGTGCGCTGCATCATCGAGCCGGGCCAGTTGAAGATCA
TGTCGACCAACGCCGATCAGGAAGAGGCGCAGGAAGAACTGGAAATCGCATACGACGGCGACAGCGTCGATATCGGGTTC
AACGTCACGTATCTGCTCGACGTGCTCGCGAACCTGAAGGTCGACACGTTGCAGGTGAGCCTGGGCGACGCCAGCTCCAG
CGCGTTGATCACGATTCCCGAGAACGACGAATTCAAGTACGTCGTCATGCCGATGCGCATCTAA

Upstream 100 bases:

>100_bases
CCGTGACAGGCGCTGCACGGCGCCGGCGGGGAGCCACGGCTGCGTGCTGGAAAGCTGTCCTGGCAAGGCGCGCAGGCCGT
TATATCAACGAAGGAACTCT

Downstream 100 bases:

>100_bases
CGCGTCCAGAAACCAGAGAACACACCAAGGGGCGCAGCGCCCCTTTGGCGTTTTTATGGTGATTTGAAAAGTCCCGAGCA
GCAACCTTGCAGCAACGCAG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MQLVKTERDNLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGDSVATTVAARKLLDIL
RAMPDGQVTLTLNDKRLTVQSGKSRFALQTLAADEFPTVAQAKDYGANLVVPQKTFRQLLGMVHFSMAQQDIRYYLNGML
LVVDGDQLMAVATDGHRLAFSSMKIEGSFQRQEVIIPRKTILELQRLLEDIDDTLKIDIAQTQVKFTFGQVELVSKLVEG
KFPDFQRVIPKSHKNQFVIGREELQRSLQRAAILTSDKFKGVRCIIEPGQLKIMSTNADQEEAQEELEIAYDGDSVDIGF
NVTYLLDVLANLKVDTLQVSLGDASSSALITIPENDEFKYVVMPMRI

Sequences:

>Translated_367_residues
MQLVKTERDNLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGDSVATTVAARKLLDIL
RAMPDGQVTLTLNDKRLTVQSGKSRFALQTLAADEFPTVAQAKDYGANLVVPQKTFRQLLGMVHFSMAQQDIRYYLNGML
LVVDGDQLMAVATDGHRLAFSSMKIEGSFQRQEVIIPRKTILELQRLLEDIDDTLKIDIAQTQVKFTFGQVELVSKLVEG
KFPDFQRVIPKSHKNQFVIGREELQRSLQRAAILTSDKFKGVRCIIEPGQLKIMSTNADQEEAQEELEIAYDGDSVDIGF
NVTYLLDVLANLKVDTLQVSLGDASSSALITIPENDEFKYVVMPMRI
>Mature_367_residues
MQLVKTERDNLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGDSVATTVAARKLLDIL
RAMPDGQVTLTLNDKRLTVQSGKSRFALQTLAADEFPTVAQAKDYGANLVVPQKTFRQLLGMVHFSMAQQDIRYYLNGML
LVVDGDQLMAVATDGHRLAFSSMKIEGSFQRQEVIIPRKTILELQRLLEDIDDTLKIDIAQTQVKFTFGQVELVSKLVEG
KFPDFQRVIPKSHKNQFVIGREELQRSLQRAAILTSDKFKGVRCIIEPGQLKIMSTNADQEEAQEELEIAYDGDSVDIGF
NVTYLLDVLANLKVDTLQVSLGDASSSALITIPENDEFKYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=362, Percent_Identity=43.646408839779, Blast_Score=308, Evalue=3e-85,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40955; Mature: 40955

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLVKTERDNLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADF
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCEEEEECCEEEEEEECCCC
GVGGDSVATTVAARKLLDILRAMPDGQVTLTLNDKRLTVQSGKSRFALQTLAADEFPTVA
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCEEEHHHCCCCCCCHH
QAKDYGANLVVPQKTFRQLLGMVHFSMAQQDIRYYLNGMLLVVDGDQLMAVATDGHRLAF
HHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCEEEE
SSMKIEGSFQRQEVIIPRKTILELQRLLEDIDDTLKIDIAQTQVKFTFGQVELVSKLVEG
EEEEECCCCCCCEEECCHHHHHHHHHHHHCCCHHEEEEEEEEEEEEEEHHHHHHHHHHCC
KFPDFQRVIPKSHKNQFVIGREELQRSLQRAAILTSDKFKGVRCIIEPGQLKIMSTNADQ
CCCCHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCH
EEAQEELEIAYDGDSVDIGFNVTYLLDVLANLKVDTLQVSLGDASSSALITIPENDEFKY
HHHHHHEEEEECCCEEEECCHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCCCCCEEE
VVMPMRI
EEEEECC
>Mature Secondary Structure
MQLVKTERDNLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADF
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCEEEEECCEEEEEEECCCC
GVGGDSVATTVAARKLLDILRAMPDGQVTLTLNDKRLTVQSGKSRFALQTLAADEFPTVA
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCEEEHHHCCCCCCCHH
QAKDYGANLVVPQKTFRQLLGMVHFSMAQQDIRYYLNGMLLVVDGDQLMAVATDGHRLAF
HHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCEEEE
SSMKIEGSFQRQEVIIPRKTILELQRLLEDIDDTLKIDIAQTQVKFTFGQVELVSKLVEG
EEEEECCCCCCCEEECCHHHHHHHHHHHHCCCHHEEEEEEEEEEEEEEHHHHHHHHHHCC
KFPDFQRVIPKSHKNQFVIGREELQRSLQRAAILTSDKFKGVRCIIEPGQLKIMSTNADQ
CCCCHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCH
EEAQEELEIAYDGDSVDIGFNVTYLLDVLANLKVDTLQVSLGDASSSALITIPENDEFKY
HHHHHHEEEEECCCEEEECCHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCCCCCEEE
VVMPMRI
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]