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The map label for this gene is prfC [H]

Identifier: 91214089

GI number: 91214089

Start: 5033363

End: 5034949

Strand: Direct

Name: prfC [H]

Synonym: UTI89_C5144

Alternate gene names: 91214089

Gene position: 5033363-5034949 (Clockwise)

Preceding gene: 91214087

Following gene: 91214090

Centisome position: 99.36

GC content: 54.25

Gene sequence:

>1587_bases
ATGACAAGCCTTTTGGCCGCAGAGGTAGCGAAAAGAAGAACCTTTGCAATCATTTCTCACCCGGACGCCGGTAAGACTAC
CATCACCGAGAAGGTGCTGCTGTTCGGACAGGCCATTCAGACCGCCGGTACAGTAAAAGGCCGTGGCTCTAACCAGCACG
CTAAGTCGGACTGGATGGAGATGGAAAAGCAGCGTGGGATCTCCATCACTACGTCTGTGATGCAGTTTCCGTATCACGAC
TGCCTGGTTAACTTGCTCGATACCCCGGGGCACGAAGACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTG
CTGCCTGATGGTTATCGACGCCGCAAAAGGTGTAGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGATA
CGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAACTGCTCGATGAAGTTGAGAACGAG
CTGAAAATCGGCTGCGCACCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTACCACCTTTATAAAGA
TGAAACCTATCTCTATCAGAGCGGTAAAGGCCACACCATTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATC
TCGACGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAGCTGGTGAAAGGCGCGTCTAATGAGTTC
GACAAAGAGCTGTTCCTTGCGGGCGAAATCACCCCGGTATTCTTTGGTACTGCGCTGGGTAACTTCGGCGTCGATCATAT
GCTGGATGGCCTGGTGGAGTGGGCGCCTGCGCCGATGCCGCGTCAGACCGATACCCGTACCGTAGAAGCGAGCGAAGATA
AATTTACCGGCTTCGTATTTAAAATTCAGGCCAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGCGTGGTA
TCCGGTAAATATGAAAAAGGCATGAAGCTGCGCCAGGTGCGCACCGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTT
TATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCTGGCGATATCCTTGGTCTGCACAACCACGGCACCATTCAGA
TCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACTTCGCGCCAGAACTGTTCCGTCGTATC
CGCCTGAAAGATCCGCTGAAGCAAAAACAGCTGCTCAAAGGGCTGGTACAGCTTTCCGAAGAGGGCGCGGTGCAGGTGTT
CCGTCCGATCTCCAACAACGATTTGATCGTTGGCGCGGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGA
GCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGGTAGAATGTGCGGACGCGAAGAAA
TTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCAT
GGTCAACCTGCGCCTGGCGCAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAA

Upstream 100 bases:

>100_bases
GATCATGTTCTGCTATGCTCTGATACCTCCAAAATGTACAATCAGCGGCATGAATTTTTCTTTGTACATATGATTGTACA
TAATCGAAGAGACAACAATT

Downstream 100 bases:

>100_bases
TTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTGTCAATTTCCCTTCCTTATTAGCCGCTTACGGAATGTTCTTAAAA
CATTCACTTTTGCTTATGTT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHD
CLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVKGASNEF
DKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVV
SGKYEKGMKLRQVRTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRRI
RLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATARWVECADAKK
FEEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH

Sequences:

>Translated_528_residues
MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHD
CLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVKGASNEF
DKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVV
SGKYEKGMKLRQVRTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRRI
RLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATARWVECADAKK
FEEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH
>Mature_527_residues
TSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDC
LVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENEL
KIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVKGASNEFD
KELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVS
GKYEKGMKLRQVRTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRRIR
LKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATARWVECADAKKF
EEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=498, Percent_Identity=28.1124497991968, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI19923640, Length=519, Percent_Identity=26.2042389210019, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI25306283, Length=402, Percent_Identity=27.8606965174129, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI25306287, Length=491, Percent_Identity=25.8655804480652, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI157426893, Length=169, Percent_Identity=28.4023668639053, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI94966754, Length=166, Percent_Identity=28.9156626506024, Blast_Score=77, Evalue=6e-14,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=100, Blast_Score=1085, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=493, Percent_Identity=26.1663286004057, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI48994988, Length=215, Percent_Identity=30.6976744186047, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=32.0512820512821, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=515, Percent_Identity=26.0194174757282, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=164, Percent_Identity=31.7073170731707, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=152, Percent_Identity=30.2631578947368, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=482, Percent_Identity=28.4232365145228, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=422, Percent_Identity=26.7772511848341, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=24.8407643312102, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=26.5822784810127, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=26.5822784810127, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24582462, Length=494, Percent_Identity=26.1133603238866, Blast_Score=166, Evalue=5e-41,
Organism=Drosophila melanogaster, GI221458488, Length=521, Percent_Identity=24.3761996161228, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=32.7044025157233, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59329; Mature: 59197

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWME
CCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
MEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVED
HHHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHH
RTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENELKIGCAPITWPIGCGKLFKG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHH
VYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVKGASNEF
HHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEASEDKFTGFVF
CCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEEE
KIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVRTAKDVVISDALTFMAGDRSHVEEAYP
EEEECCCCCHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
GDILGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRRIRLKDPLKQKQLLKGLVQLSE
CCEEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
EGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATARWVECADAKK
CCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FEEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH
HHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWME
CHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
MEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVED
HHHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHH
RTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENELKIGCAPITWPIGCGKLFKG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHH
VYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVKGASNEF
HHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEASEDKFTGFVF
CCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEEE
KIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVRTAKDVVISDALTFMAGDRSHVEEAYP
EEEECCCCCHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
GDILGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRRIRLKDPLKQKQLLKGLVQLSE
CCEEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
EGAVQVFRPISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVATARWVECADAKK
CCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FEEFKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTREH
HHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA