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The map label for this gene is odhL [H]

Identifier: 91213586

GI number: 91213586

Start: 4528460

End: 4529878

Strand: Direct

Name: odhL [H]

Synonym: UTI89_C4633

Alternate gene names: 91213586

Gene position: 4528460-4529878 (Clockwise)

Preceding gene: 91213585

Following gene: 91213587

Centisome position: 89.39

GC content: 52.92

Gene sequence:

>1419_bases
ATGAGTACAATTTTTGATGTGGCAGTTATGGGGGGTGGGCCAGGTGGTTACGTAGCGGCACTGCGGGCTGCGCAGAATGG
GCTTTCCGTAGTATGTATTGATGATGGTGTTAATGCACAGGGCGAACCTTCGCCGGGCGGTACGTGTCTCAATGTCGGCT
GTATTCCGTCAAAATCGTTGCTGCAATCTTCCGAGCTGTACGCGCAAGTACAGCATGAGGCAAGCATTCATGGCGTTAAC
GTTGAAGGCGTATCTTTCAATGCTGCCGCGATGATTCAACGTAAAGATGCCATAGTAAGCCGCCTGACAATGGGCATCAG
CTTGCTGTTTAAGAAAAATAAAGTGAAGCACTTGTGCGGCCTCGCAACGTTAGAACGTGCTCAGGATGAGATTTGGCAAT
TACGCGTAAACGATCAACACATTCACGCCCGTAATGTAGTGATTGCAACAGGTTCTCAGCCGCGTCAGCTGCCTGGCGTT
ACCATTGATAATCAACAGATCCTCGACAATCGTGGCGCCCTGGCATTGAGCGAAGTGCCGCCGCGTCTCGGTGTGATCGG
CGCTGGCGTGATTGGCCTTGAACTGGGGTCTGTGTGGAATCGGGTCGGTTCAGATGTCACGCTGCTGGAGATGGCGCCAA
CGTTCTTGCCTGCACTGGAAGCTCGCCTGTCGAACGAAGTCCGTAAAGCAATGATTGCCAGTGGGATGAAAATGCAACTG
GCAGTAGAGATTGAGGCTATTGAACAACGGGATGACGGTGTGCATGTGCGCTGGCGGCAGGGTGAGAAGCGAGAAGAAAG
CCGCTTCGATAAATTAATTCTTGCCATTGGACGTGTACCGCGACTTTCTGGTGTCGATCTCGTGCAACTCGGTCTTGAAG
CCGATAACCGTGGCGGTATTGCCGTCGATAACTTATGCCGTACCGGGAAAGCCGGGCTGTGGGCAATTGGTGATGTCGTG
CGAGGGCCGATGCTGGCGCATAAAGCGATGGCTGAAGGTGTGGTGGTTGCGGATCAAATTGCCGGACTGGCGGTAGAGCC
GATTAATTTTGCGCTGATCCCTTCGGTGATTTACACCCAACCGGAAGTTGCGTGGGTAGGGGAAAACGAGGCCTCACTGA
AAGCCGCTGGCAGAGTCTTTAACAAAGGAAATTCTCTGTTTGCCGGTAACGGTCGCGCCTTAGCATTAGGCCAGGAAGGT
GGGCGTTGCACGTTATACAGCGATAAACACACTGACCGCGTACTTGGCGGAGCGATTGTTGGCCCGCAGGCATCGGAGCT
GATCAATGAAATTGCGCTGGCGATGACTTTTAGCGCCTCTGGCGAGGATATTGCCTGTGCGATCCATGCGCATCCGACCT
TAAGCGAAGTCATCCACGAAGCAGCAATGGCCTTGAATAATAAAGCATTACACGGATAA

Upstream 100 bases:

>100_bases
GCATTATCGATGGTCAGGAAGCCGTGCAGACATTGGTGGCAATACGTGAGCTACTGGAATCACCGGAACAACTGTTGCTG
GATCTTTAAGGAGGGCGCCG

Downstream 100 bases:

>100_bases
CACATACCACTGAGGGAATATATGAATTTACATGAGTATCAGGCCAAATCGTTACTGGCTGGCATGGGGATGCCGTGTCC
AAAAGAGATAGCCATCCAAC

Product: subunit of 2-oxoglutarate dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPSPGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVN
VEGVSFNAAAMIQRKDAIVSRLTMGISLLFKKNKVKHLCGLATLERAQDEIWQLRVNDQHIHARNVVIATGSQPRQLPGV
TIDNQQILDNRGALALSEVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPALEARLSNEVRKAMIASGMKMQL
AVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAIGRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVV
RGPMLAHKAMAEGVVVADQIAGLAVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGRALALGQEG
GRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHAHPTLSEVIHEAAMALNNKALHG

Sequences:

>Translated_472_residues
MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPSPGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVN
VEGVSFNAAAMIQRKDAIVSRLTMGISLLFKKNKVKHLCGLATLERAQDEIWQLRVNDQHIHARNVVIATGSQPRQLPGV
TIDNQQILDNRGALALSEVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPALEARLSNEVRKAMIASGMKMQL
AVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAIGRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVV
RGPMLAHKAMAEGVVVADQIAGLAVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGRALALGQEG
GRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHAHPTLSEVIHEAAMALNNKALHG
>Mature_471_residues
STIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPSPGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVNV
EGVSFNAAAMIQRKDAIVSRLTMGISLLFKKNKVKHLCGLATLERAQDEIWQLRVNDQHIHARNVVIATGSQPRQLPGVT
IDNQQILDNRGALALSEVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPALEARLSNEVRKAMIASGMKMQLA
VEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAIGRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVR
GPMLAHKAMAEGVVVADQIAGLAVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGRALALGQEGG
RCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHAHPTLSEVIHEAAMALNNKALHG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=42.7659574468085, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI50301238, Length=426, Percent_Identity=25.1173708920188, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI291045266, Length=441, Percent_Identity=25.8503401360544, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI22035672, Length=471, Percent_Identity=26.1146496815287, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI33519430, Length=468, Percent_Identity=23.7179487179487, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI33519428, Length=468, Percent_Identity=23.7179487179487, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI33519426, Length=468, Percent_Identity=23.7179487179487, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI148277065, Length=468, Percent_Identity=23.7179487179487, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI148277071, Length=468, Percent_Identity=23.7179487179487, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI291045268, Length=433, Percent_Identity=24.7113163972286, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI1786307, Length=458, Percent_Identity=38.646288209607, Blast_Score=284, Evalue=7e-78,
Organism=Escherichia coli, GI87082354, Length=468, Percent_Identity=26.9230769230769, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1789915, Length=416, Percent_Identity=26.4423076923077, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87081717, Length=469, Percent_Identity=26.4392324093817, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32565766, Length=469, Percent_Identity=42.4307036247335, Blast_Score=347, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI17557007, Length=477, Percent_Identity=25.9958071278826, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=441, Percent_Identity=26.7573696145125, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=458, Percent_Identity=27.7292576419214, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=482, Percent_Identity=26.1410788381743, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17559934, Length=193, Percent_Identity=29.0155440414508, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=39.6265560165975, Blast_Score=333, Evalue=3e-92,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=29.4736842105263, Blast_Score=193, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=26.7665952890792, Blast_Score=150, Evalue=4e-37,
Organism=Drosophila melanogaster, GI21358499, Length=466, Percent_Identity=41.4163090128755, Blast_Score=341, Evalue=7e-94,
Organism=Drosophila melanogaster, GI24640553, Length=485, Percent_Identity=26.5979381443299, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24640549, Length=485, Percent_Identity=26.5979381443299, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24640551, Length=485, Percent_Identity=26.5979381443299, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17737741, Length=478, Percent_Identity=24.4769874476987, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49908; Mature: 49777

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPSPGGTCLNVGCIPSKSL
CCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCHHH
LQSSELYAQVQHEASIHGVNVEGVSFNAAAMIQRKDAIVSRLTMGISLLFKKNKVKHLCG
HHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHH
LATLERAQDEIWQLRVNDQHIHARNVVIATGSQPRQLPGVTIDNQQILDNRGALALSEVP
HHHHHHHHHHHEEEEECCCEEEEEEEEEECCCCCCCCCCEEECCHHHHCCCCCEEECCCC
PRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPALEARLSNEVRKAMIASGMKMQL
CCCCEEECCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
AVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAIGRVPRLSGVDLVQLGLEADNRGGI
EEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCE
AVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGVVVADQIAGLAVEPINFALIPSVIYTQ
EHHHHHHCCCCCEEEHHHHHCCHHHHHHHHHCCEEEEHHHCCEEECCCCEEEECHHHCCC
PEVAWVGENEASLKAAGRVFNKGNSLFAGNGRALALGQEGGRCTLYSDKHTDRVLGGAIV
CCEEEECCCCHHHHHHHHHHCCCCEEEECCCCEEEEECCCCEEEEECCCCCCHHHCCEEE
GPQASELINEIALAMTFSASGEDIACAIHAHPTLSEVIHEAAMALNNKALHG
CCCHHHHHHHHHHEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPSPGGTCLNVGCIPSKSL
CCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCHHH
LQSSELYAQVQHEASIHGVNVEGVSFNAAAMIQRKDAIVSRLTMGISLLFKKNKVKHLCG
HHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHH
LATLERAQDEIWQLRVNDQHIHARNVVIATGSQPRQLPGVTIDNQQILDNRGALALSEVP
HHHHHHHHHHHEEEEECCCEEEEEEEEEECCCCCCCCCCEEECCHHHHCCCCCEEECCCC
PRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPALEARLSNEVRKAMIASGMKMQL
CCCCEEECCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
AVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAIGRVPRLSGVDLVQLGLEADNRGGI
EEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCE
AVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGVVVADQIAGLAVEPINFALIPSVIYTQ
EHHHHHHCCCCCEEEHHHHHCCHHHHHHHHHCCEEEEHHHCCEEECCCCEEEECHHHCCC
PEVAWVGENEASLKAAGRVFNKGNSLFAGNGRALALGQEGGRCTLYSDKHTDRVLGGAIV
CCEEEECCCCHHHHHHHHHHCCCCEEEECCCCEEEEECCCCEEEEECCCCCCHHHCCEEE
GPQASELINEIALAMTFSASGEDIACAIHAHPTLSEVIHEAAMALNNKALHG
CCCHHHHHHHHHHEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]