Click here to switch to the map view.

The map label for this gene is sucB [H]

Identifier: 91213585

GI number: 91213585

Start: 4527393

End: 4528448

Strand: Direct

Name: sucB [H]

Synonym: UTI89_C4632

Alternate gene names: 91213585

Gene position: 4527393-4528448 (Clockwise)

Preceding gene: 91213583

Following gene: 91213586

Centisome position: 89.37

GC content: 54.26

Gene sequence:

>1056_bases
GTGATTGCCGAGCTGGAAACCGATAAAGTCATTCTGGAAATACCGGCCCCGCACGATGGCGTGTTAAGCAATATTATCGT
CAGCGAAGGTAGCACGGTCACGTCCGCGCAACTGTTGGCGCATCTTAAGCCGCAAGCTGTCATAGAAGAAACCGTCACAC
CAGTCACCGAAACCCTGGCGATGCCTTCCGCACGGCTGGAGGCACAGCGTAGTGGTGTCGAACTTGCTGACGTTGCGGGG
AGCGGGAGAAATGGACGTATCCTGAAAGAGGATGTACAGCGCGTTACTCCTGCACCAGTCATTCAGCCTGAACGGGTGGC
GGAGATCGCGCCAGCTAAACCCTTGACACCGGGTGCTCGTCAGGAACGTCGTGAGCCGATGTCGCGCTTACGTCAGCGAA
TTGCTGAGCGTCTGCTGGCTTCCCAACAAAATAACGCCATTCTTACCACATTCAACGAAGTAAACATGCAGAGCGTGATG
GATTTACGCACCCGCTGGAAAGATCGTTTTGCCGAGAAGCACGGCGTGAAGCTGGGCTTTATGTCCTTCTTTGTTAAGGC
CGTTACCCGGGCGCTGGAGCGCTTCCCTGTAGTGAATGCCAGCGTTGATGGCAACGAGATTATCTGGCGTGATTATTGCG
ATATCGGCATTGCAGTGAGCAGCAACCGCGGCCTGGTAGTGCCTGTGTTACGCAATGCGCAATCACTCTCTTTGGTGGAA
ATTGAACGGCAAATTGCCGAATACGCCACACAGGCACGCAATGGCAAATTGCCGCTGGAGGCGTTACAGGGCGGCACGTT
CTCTATCACCAACGGCGGTACTTTTGGTTCGATGATGTCAACGCCAATCATTAACCCGCCGCAGTCGGCCATTCTTGGGA
TGCATGCCATCACGCCGCGCCCGGTCGCAGAAAACGGCCAGGTTGTTATTCGTCCCATGATGTACCTTGCTCTCAGCTAT
GACCATCGCATTATCGATGGTCAGGAAGCCGTGCAGACATTGGTGGCAATACGTGAGCTACTGGAATCACCGGAACAACT
GTTGCTGGATCTTTAA

Upstream 100 bases:

>100_bases
TATGATCGAAATTACTGTCCCTGTATTACCGGAATCTGTCACGGAAGGCACGCTGACAACCTGGTGTAAACAAGAAGGCG
AGCACGTAAAACGCGATGAT

Downstream 100 bases:

>100_bases
GGAGGGCGCCGATGAGTACAATTTTTGATGTGGCAGTTATGGGGGGTGGGCCAGGTGGTTACGTAGCGGCACTGCGGGCT
GCGCAGAATGGGCTTTCCGT

Product: dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MIAELETDKVILEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQRSGVELADVAG
SGRNGRILKEDVQRVTPAPVIQPERVAEIAPAKPLTPGARQERREPMSRLRQRIAERLLASQQNNAILTTFNEVNMQSVM
DLRTRWKDRFAEKHGVKLGFMSFFVKAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVE
IERQIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRPVAENGQVVIRPMMYLALSY
DHRIIDGQEAVQTLVAIRELLESPEQLLLDL

Sequences:

>Translated_351_residues
MIAELETDKVILEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQRSGVELADVAG
SGRNGRILKEDVQRVTPAPVIQPERVAEIAPAKPLTPGARQERREPMSRLRQRIAERLLASQQNNAILTTFNEVNMQSVM
DLRTRWKDRFAEKHGVKLGFMSFFVKAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVE
IERQIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRPVAENGQVVIRPMMYLALSY
DHRIIDGQEAVQTLVAIRELLESPEQLLLDL
>Mature_351_residues
MIAELETDKVILEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQRSGVELADVAG
SGRNGRILKEDVQRVTPAPVIQPERVAEIAPAKPLTPGARQERREPMSRLRQRIAERLLASQQNNAILTTFNEVNMQSVM
DLRTRWKDRFAEKHGVKLGFMSFFVKAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVE
IERQIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRPVAENGQVVIRPMMYLALSY
DHRIIDGQEAVQTLVAIRELLESPEQLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=358, Percent_Identity=43.854748603352, Blast_Score=285, Evalue=4e-77,
Organism=Homo sapiens, GI110671329, Length=387, Percent_Identity=28.6821705426357, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI31711992, Length=399, Percent_Identity=28.5714285714286, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI203098816, Length=258, Percent_Identity=31.0077519379845, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI203098753, Length=258, Percent_Identity=31.3953488372093, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.1614906832298, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786946, Length=370, Percent_Identity=50, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1786305, Length=394, Percent_Identity=29.4416243654822, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25146366, Length=370, Percent_Identity=41.3513513513514, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17560088, Length=301, Percent_Identity=31.8936877076412, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=30.4918032786885, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17537937, Length=389, Percent_Identity=25.1928020565553, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=360, Percent_Identity=43.0555555555556, Blast_Score=295, Evalue=6e-81,
Organism=Saccharomyces cerevisiae, GI6324258, Length=419, Percent_Identity=28.8782816229117, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24645909, Length=234, Percent_Identity=53.8461538461538, Blast_Score=267, Evalue=9e-72,
Organism=Drosophila melanogaster, GI18859875, Length=303, Percent_Identity=30.3630363036304, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=31.5789473684211, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=31.5789473684211, Blast_Score=121, Evalue=6e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 38517; Mature: 38517

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAELETDKVILEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLA
CCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
MPSARLEAQRSGVELADVAGSGRNGRILKEDVQRVTPAPVIQPERVAEIAPAKPLTPGAR
CCHHHHHHHHCCCEEEHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHH
QERREPMSRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRTRWKDRFAEKHGVKLGF
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHH
MSFFVKAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVE
HHHHHHHHHHHHHHCCCEECCCCCCEEEEEECCCEEEEEECCCCEEEEECCCCCCHHHHH
IERQIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPR
HHHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHCCCCC
PVAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL
CCCCCCCEEEHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHCHHHHHHCC
>Mature Secondary Structure
MIAELETDKVILEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLA
CCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
MPSARLEAQRSGVELADVAGSGRNGRILKEDVQRVTPAPVIQPERVAEIAPAKPLTPGAR
CCHHHHHHHHCCCEEEHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHH
QERREPMSRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRTRWKDRFAEKHGVKLGF
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHH
MSFFVKAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVE
HHHHHHHHHHHHHHCCCEECCCCCCEEEEEECCCEEEEEECCCCEEEEECCCCCCHHHHH
IERQIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPR
HHHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHCCCCC
PVAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL
CCCCCCCEEEHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]