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The map label for this gene is ascB [H]

Identifier: 91212075

GI number: 91212075

Start: 2999480

End: 3000904

Strand: Direct

Name: ascB [H]

Synonym: UTI89_C3078

Alternate gene names: 91212075

Gene position: 2999480-3000904 (Clockwise)

Preceding gene: 91212074

Following gene: 91212086

Centisome position: 59.21

GC content: 52.49

Gene sequence:

>1425_bases
ATGTCAGTATTTCCGCAAGGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGCGCGTACCGTGAAGGCGG
CAAAGGGCTGACCACCGTCGATATGATCCCGCACGGCGAACATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTC
AGTTGCGCGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATG
GCAGAGATGGGATTCAAGGTTTTTCGCACCTCGATTGCCTGGAGCCGCCTATTTCCGCAGGGCGATGAACTGACGCCCAA
TCAGCAGGGCATTGCTTTTTACCGCGCGGTATTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGTC
ACTTCGACGTGCCGATGCATCTGGTTACTGAATATGGCTCCTGGCGTAACCGCAAGCTGGTAGAGCTTTTCAGCCGCTAC
GCCCGGACCTGCTTTGAAGCATTTGATGGTCTGGTGAAATACTGGCTTACCTTCAATGAAATCAACATTATGTTGCATAG
CCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGGGAAAATCAGGATCAGGTGAAATATCAGGCTGCGCATCACCAGC
TGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGTTGTATGCTGGCGGGCGGT
AACTTCTACCCTTACAGCTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGATCGGGAAAACCTGTTTTTTATCGA
TGTGCAGGCGCGGGGCGCGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCGACAAAGCACCGA
GCGATGATGAGATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATG
AACGCCAACAACTGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAACCCGTATCTGCAAGTGAGCGACTGGGGCTGGGG
TATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGACCGTTATCAGAAGCCACTGTTTCTGGTGGAAAACG
GCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAAATTAACGACGACTATCGCATCAGCTATTTACGCGAACATATC
CGCGCAATGGGCGAAGCGATTGCAGACGGCATTCCGCTGATGGGCTACACCACATGGGGCTGTATTGATTTAGTTTCCGC
CTCTACGGGTGAAATGAGCAAACGCTACGGTTTTGTCTACGTAGACCGTGACGACGCAGGCAACGGCACGCTGACGCGCA
CGCGTAAGAAATCGTTCTGGTGGTATAAAAAAGTGATTGCCAGTAACGGTGAAGATTTAGAGTAA

Upstream 100 bases:

>100_bases
TTGAGGATATTCCTGTTGAGGAAGCGACTGCCGAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTA
AGTCTTAATTGAGGATGAAA

Downstream 100 bases:

>100_bases
GTAACCGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGGCCCGATAAGCGTTGCGCATCGGGCAATCTTGGGT
TTGGCATCAGTCAAAAATCA

Product: cryptic 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSVFPQGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALM
AEMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRAVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRY
ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPSDDEILKNTVDFVSFSYYASRCASAEM
NANNCSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHI
RAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE

Sequences:

>Translated_474_residues
MSVFPQGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALM
AEMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRAVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRY
ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPSDDEILKNTVDFVSFSYYASRCASAEM
NANNCSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHI
RAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
>Mature_473_residues
SVFPQGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMA
EMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRAVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRYA
RTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGGN
FYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPSDDEILKNTVDFVSFSYYASRCASAEMN
ANNCSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHIR
AMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE

Specific function: Can hydrolyze salicin, cellobiose, and probably arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=499, Percent_Identity=30.4609218436874, Blast_Score=182, Evalue=8e-46,
Organism=Homo sapiens, GI32481206, Length=495, Percent_Identity=28.4848484848485, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI13273313, Length=487, Percent_Identity=27.7207392197125, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI24497614, Length=490, Percent_Identity=27.5510204081633, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI28376633, Length=491, Percent_Identity=24.4399185336049, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1789070, Length=474, Percent_Identity=97.4683544303797, Blast_Score=975, Evalue=0.0,
Organism=Escherichia coli, GI2367270, Length=474, Percent_Identity=54.6413502109705, Blast_Score=542, Evalue=1e-155,
Organism=Escherichia coli, GI2367174, Length=478, Percent_Identity=51.255230125523, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17552856, Length=487, Percent_Identity=30.1848049281314, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17539390, Length=499, Percent_Identity=30.4609218436874, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI21356577, Length=499, Percent_Identity=25.250501002004, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53845; Mature: 53714

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFPQGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEF
CCCCCCCCEECCEEECCCCCCCHHCCCCCCEEEEECCCCCCEEEEEECCHHHCCCCCCCC
YPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRAVFEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
CKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRYARTCFEAFDGLVKYWLTFNE
HHHCCCCHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
INIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
EEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPSDDEILK
CCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEECCCCCHHHHHH
NTVDFVSFSYYASRCASAEMNANNCSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMM
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEHHHH
YDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHIRAMGEAIADGIPLMGYTTWG
HHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
HHHHHCCCCCCHHHHCCEEEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SVFPQGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEF
CCCCCCCEECCEEECCCCCCCHHCCCCCCEEEEECCCCCCEEEEEECCHHHCCCCCCCC
YPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRAVFEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
CKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRYARTCFEAFDGLVKYWLTFNE
HHHCCCCHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
INIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
EEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPSDDEILK
CCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEECCCCCHHHHHH
NTVDFVSFSYYASRCASAEMNANNCSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMM
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEHHHH
YDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHIRAMGEAIADGIPLMGYTTWG
HHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
HHHHHCCCCCCHHHHCCEEEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]